Klamath Experiment Station Alfalfa Variety Trial, 1996-97 R.L. Dovel and J. Rainey' A bstract A trial including 28 released and experimental alfalfa varieties was established in August, 1996. Varieties were arranged in a randomized complete block design with four replications. Plots were 5x 20 feet with 5-foot borders and alleyways. The crop was sprinklerirrigated with a solid-set system. Plants were allowed to grow through the first growing season without cutting. Alfalfa was harvested in 1997 when plants reached early bud stage. The crop was harvested using a flail harvester with a 3-foot wide head. Three cuttings were also taken in 1997. There was no significant difference in yield among varieties in the first and second cuttings. There were significant differences in the third cutting, but not in total forage yield. The collection of only one year of data is inadequate to provide an estimate of the relative yield potential of the varieties in this trial. An additional 2 years of data are needed to adequately assess yield potential and to evaluate stand survival in this environment. Introduction Alfalfa is a major forage commodity in the Klamath Basin. It is grown on over 40,000 acres in Klamath County alone. Major markets for Klamath Basin alfalfa are dairies, cattle ranches, and horse farms in Oregon and California. Premium quality hay suitable for the dairy hay market requires timely cutting, good weed control, and an adequate stand. Little difference in forage quality has been seen between alfalfa varieties in the past. Breeding programs are striving to improve alfalfa quality. Varieties reportedly superior in quality are beginning to be marketed; however, management still appears to have more effect on forage quality than varietal differences. Few pests attack alfalfa in the Klamath Basin. The main diseases present are bacterial wilt and phytophthora root rot. Verticillium wilt has not been found in the basin, but it occurs in many surrounding areas. The main insect pest is the alfalfa weevil. Some breeding programs are beginning to select for resistance to this pest. Pest resistances are important variety selection criteria. Winter hardiness is important in selecting a variety for the Klamath Basin. Winter hardiness has been closely linked with fall dormancy ratings; however, less dormant varieties have experienced reasonable stand longevity in recent years, perhaps due to relatively mild winters. Local variety trials are being used to develop empirical winter hardiness and stand persistence measurements to supplement fall dormancy ratings as a '/Associate Professor and Biological Sciences Research Technician III, respectively, Klamath Experiment Station, Klamath Falls, OR. Acknowledgments: We acknowledge gratefully the suppport of these studies from the following companies: ABI Alfalfa; Allied Seed; Barenbrug, USA; Dairyland Seed; DeKalb Seed; Grasslands West; Great Lakes Hybrids; Great Plains Research; Loshe Mill; Northrup King Co.; Pioneer Hi-Bred International; SeedTec International; Union Seed Co.; and W-L Research. 126 Klamath Experiment Station 1997 Klamath Experiment Station Alfalfa Variety Trial, 1996-97 measure of variety hardiness. Forage yield is a function of a complex set of interactions between the alfalfa plant and its environment. Variety trials conducted at Klamath Experiment Station (KES) provide alfalfa producers locally developed data on the yield potential and persistence of new alfalfa varieties. An alfalfa variety trial established at KES in 1996 is the subject of this report. Procedures A trial including 28 released and experimental alfalfa varieties was established in August, 1996. Varieties were arranged in a randomized complete block design with four replications. Soil samples from the field were analyzed and the appropriate fertilizer applied before planting. Seed was drilled to a depth of 1/4 inch at a rate of 20 lb/acre using a modified Kincaid drill. Plots were 5x 30 feet with 5-foot borders and alleyways. The crop was sprinkler-irrigated with a solid-set system. Plants were allowed to grow through the first growing season without cutting. Alfalfa was harvested in subsequent years when plants reached early bud stage. The crop was harvested using a flail harvester with a 3-foot wide head. All yields are reported on a dry weight basis. Three cuttings were also taken in 1997. Results and Discussion cutting, but not in total forage yield. Failure to distinguish between varieties was not a result of high variability. Coefficients of variation were 10,11, 8, and 6 percent for first, second, and third cuttings, and total yield, respectively. In the first cutting, forage production ranged from 2.90 ton/acre for Excalibur II to 2.33 ton/acre for 5246. This is a difference from lowest to highest of just over one-half ton/acre. A similar range in yields was seen in the second and third harvests as well, 0.42 and 0.54 ton/acre, respectively. Differences in forage quality were not detected in the first harvest year despite low variation as indicated by coefficients of variation (CVs) (Table 2). The selection of an appropriate alfalfa variety should be based on other factors in addition to yield and quality. Disease and pest resistance as well as winter hardiness are two very important factors. Varieties selected for the Klamath Basin should be resistant to bacterial wilt, phytophthora root rot, verticillium wilt, and in some areas, stem nematode. Resistance to root knot nematode may also be a factor when in rotation with potatoes. In the past, fall dormancy ratings of 2 to 3 have been recommended for the Klamath Basin; however, recent varieties in groups 4 and even 5 have persisted over a 4-year period. Fall dormancy and pest resistance ratings of varieties included in this trial are provided in Table 3. The collection of only one year of data is inadequate to provide an estimate of the relative yield potential of the varieties in this trial. An additional 2 years of data are needed to adequately assess yield potential and to evaluate stand survival in this environment. There was no significant difference in yield among varieties in the first and second cuttings in 1997 (Table 1). There were significant (P = 0.05) differences in the third Klamath Experiment Station 1997 127 Klamath Experiment Station Table 1. Alfalfa forage yield of 28 varieties planted at the KES, Klamath Falls, OR, 1996. Entry Variety Company Cut 1 Cut 2 Cut 3 Total Rank ton / A Rushmore Aspen Innovator +Z Affinity + Z ABI 9352 Novartis Seeds Eureka Seeds America's Alfalfa America's Alfalfa America's Alfalfa 2.64 2.74 2.70 2.52 2.44 1.47 1.51 1.57 1.60 1.58 1.99 1.81 2.05 1.97 1.96 6.10 6.06 6.32 6.09 5.98 20 23 13 21 24 6 7 8 9 10 LM-331 H 154 LM 459 Accord DK127 Loshe Mill Loshe Mill Loshe Mill Union Seed/Chemgro Dekalb Genetics Corp. 2.72 2.54 2.41 2.77 2.75 1.44 1.65 1.79 1.70 1.58 1.96 1.71 1.94 1.86 1.92 6.12 5.90 6.14 6.33 6.25 19 27 18 10 15 11 12 13 14 15 5396 5246 Extend Charger Webfoot MPR Pioneer Hi-Breed Int Pioneer Hi-Breed Int Grasslands West Grasslands West Great Lakes Hybrids 2.72 2.33 2.85 2.74 2.88 1.68 1.42 1.44 1.60 1.47 1.98 1.95 2.03 1.88 2.03 6.38 5.70 6.32 6.22 6.38 5 28 14 16 7 16 17 18 19 20 Excalibur Magnum III Oneida VR Vernal Vernema Allied Seed Dairyland Public Public Public 2.90 2.77 2.72 2.8 2.58 1.69 1.61 1.72 1.44 1.55 1.98 1.95 2.01 1.94 1.83 6.57 6.33 6.45 6.18 5.96 1 11 2 17 25 21 22 23 24 25 W45 HayGrazer WL 252 HQ Blazer Blazer XL Public Great Plains Research WL Research Inc. Croplan Genetics Croplan Genetics 2.48 2.69 2.79 2.67 2.55 1.44 1.54 1.59 1.56 1.53 1.99 2.10 2.06 2.18 1.99 5.91 6.33 6.44 6.41 6.07 26 12 3 4 22 26 27 28 Baralfa 54 Baralfa 32 IQ Ranger Barenbrug, USA Barenbrug, USA Public 2.61 2.72 2.62 1.84 1.63 1.51 1.90 2.02 2.25 6.35 6.37 6.38 9 8 6 2.67 10 NS 1.58 11 NS 1.97 8 0.22 6.22 6 NS 1 2 3 4 5 - Mean CV (%) LSD (P = 0.05) 128 Klamath Experiment Station 1997 Klamath Experiment Station Table 2. 1997 alfalfa forage quality as measured by crude protein (CP), acid detergent fiber (ADF), neutral detergent fiber (NDF), total digestible nutrients (TDN), and relative feed value (RFV), of 28 varieties planted at KES, Klamath Falls, OR, 1996. RFV Variety Company CP ADF NDF TDN 1 2 3 4 5 Rushmore Aspen Innovator +Z Affinity + Z ABI 9352 Novartis Seeds Eureka Seeds America's Alfalfa America's Alfalfa America's Alfalfa 20.8 23.0 22.6 22.8 23.3 36.7 34.0 33.9 32.9 32.8 45.5 43.0 43.4 44.0 43.0 54.2 57.3 57.4 59.0 58.7 123.5 135.4 135.1 134.3 137.4 6 7 8 9 10 LM-331 H 154 LM 459 Accord DK127 Loshe Mill Loshe Mill Loshe Mill Union Seed/Chemgro Dekalb Genetics Corp. 21.9 21.4 21.0 22.8 24.1 35.4 35.3 36.0 33.6 32.3 45.0 45.8 45.8 42.4 41.2 55.7 55.8 55.0 57.8 59.2 127.2 125.2 124.4 138.9 144.6 Pioneer Hi-Breed Int Pioneer Hi-Breed Int Grasslands West Grasslands West Great Lakes Hybrids 22.5 22.5 21.9 21.3 21.7 33.5 33.6 35.1 35.8 34.0 43.2 43.8 44.8 44.9 44.4 57.8 57.7 56.1 55.2 57.3 135.6 133.4 128.2 126.6 131.3 11 5396 12 5246 13 Extend 14 Charger 15 Webfoot MPR 16 17 18 19 20 Excalibur II Magnum III Oneida VR Vernal Vernema Allied Seed Dairyland Public Public Public 22.5 22.6 22.5 22.7 21.3 34.2 33.6 33.3 33.6 35.4 43.6 42.7 42.4 42.6 44.4 57.0 57.8 58.0 57.7 55.7 133.3 137.2 138.3 137.2 128.9 21 22 23 24 25 W45 HayGrazer WL 252 HQ Blazer Blazer XL Public Great Plains Research WL Research Inc. Croplan Genetics Croplan Genetics 21.2 22.9 23.4 22.0 23.6 35.7 33.7 32.6 34.4 32.7 45.4 43.0 42.4 44.6 42.3 55.3 57.6 58.9 56.8 58.8 125.7 136.5 139.8 130.0 139.6 Barenbrug, USA Barenbrug, USA Public 23.1 21.9 21.6 33.1 34.7 35.1 42.1 45.0 44.1 58.2 56.4 56.0 140.3 128.9 130.7 22.3 8 NS 34.2 7 NS 43.7 6 NS 57.1 5 NS 133.0 9 NS 26 Baralfa 54 27 Baralfa 32 IQ 28 Ranger Mean CV (%) LSD (P = 0.05) Klamath Experiment Station 1997 129 Klamath Experiment Station Table 3. Alfalfa variety fall dormancy rating (1-D); and resistance to bacterial wilt (BW), verticillium wilt (VW), fusarium wilt (FW), anthracnose (AN), phytophthera root rot (PRR), spotted alfalfa aphid (SAA), peas aphid (PA), blue alfalfa aphid (BAA), stem nematode (SN), aphanomyces root rot race 1 (APH), Southern root knot nematode (SKRN), and Northern root knot nematode (NKRN). Data taken from Certified Seed Council's "Fall Dormancy and Pest Resistance Ratings for Alfalfa Varieties", 1997/98 Edition unless otherwise stated. FD BW VW FW AN PRR SAA PA Rushmore Aspen Innovator +Z Affinity + Z ABI 9352 1 4 4 3 4 5 HR HR HR HR R R R HR HR HR HR HR HR HR HR HR HR HR HR HR HR HR HR HR R HR HR MR HR HR R R MR LM-331 1 H-154 LM 459 Accord DK127 4 5 5 4 3 R R R MR HR HR MR R R HR HR HR HR R R HR HR R R R HR HR MR HR HR HR MR HR HR HR HR 5396 1 5246 Extend 3 3 3 HR R HR HR R R R R HR R HR HR R R HR R R R R Charger 1 Webfoot MPR 3 4 HR HR R HR HR HR HR HR HR HR Excalibur II 1 Magnum III Oneida VR Vernal Vernema 4 3 2 4 HR R R R MR R MR HR HR R HR MR HR MR MR HR R MR W45 1 HayGrazer WL 252 HQ Blazer Blazer XL 5 4 2 3 3 MR HR HR HR R LR R HR LR R HR HR HR R HR R HR LR HR R HR MR HR Baralfa 54' 5 R R HR HR Baralfa 32 IQ / 3 HR R HR HR Ranger' 3 LR Variety 1 MR / Data based on company or breeder information. 130 Klamath Experiment Station 1997 SN APH S MR R R R R HR R R R HR R R MR R MR MR HR R MR R R R R R MR MR R LR HR R R R HR MR R R MR SRKN NRKN R HR R R MR R R HR MR MR MR LR MR R BAA LR MR HR R R MR R R HR R R HR R HR HR HR MR R HR HR R R R MR HR R MR R R HR MR
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