Klamath Experiment Station Effect of Seedpiece Size on Performance of Three Potato Varieties, 1997 K.A. Rykbost', K. Locket, and J. Maxwell' A bstract Seedpiece size is an important factor in the vigor and productivity of potato crops. Varieties with limited numbers of eyes, such as Shepody, require care in seed cutting to avoid seedpieces with no eyes. Weak plants or plants that are slow to emerge are often associated with small seedpieces. A survey was conducted of cut seed lots from commercial potato operations in the Klamath Basin. Survey results showed poor uniformity in seedpiece size and a trend for undersize seedpieces in most seed lots examined. Experiments were conducted in 1995, 1996, and 1997 to evaluate the effects of seedpiece size on the performance of Russet Burbank. Russet Norkotah, and Century Russet varieties. Weighed seed tubers were four-cut to obtain seedpiece sizes of 3/4,1-1/4,1-3/4, 2-1/4, and 2-3/4 oz. Small seedpieces resulted in delayed emergence and reduced stem numbers in all varieties. Effects of seedpiece size on yield and tuber size distribution were greatest for Century Russet and least for Russet Norkotah but were different for all varieties over the three-year study. Increasing seedpiece size increased yield of U.S. No. I s and total yield but reduced tuber size. An economic analysis. based on crop values for fresh market use, found optimum seedpiece size of 2-1/4 oz for Russet Burbank and 1-3/4 oz for Russet Norkotah and Century Russet. Manipulation of seedpiece size offers a means to obtain tuber size distribution appropriate for specific market requirements. Introduction Observations of cut seed from numerous potato grower operations in several production regions in the U.S. and Canada have consistently shown poor uniformity of seedpiece size and a trend for use of small seedpieces. With the introduction of new varieties that typically have fewer eyes than Russet Burbank, missing plants because of seedpieces with no eyes has become more common, particularly in the Shepody variety. Examination of small and weak plants in commercial fields frequently shows that weak plants originate from a small seedpiece. This study was initiated in 1994 to evaluate local seed cutting practices and determine the response of crops to a range of seedpiece sizes under local conditions. The 1994 study was limited to the Russet Burbank variety. A uniform experimental design was established in 1995 to expand the study to include Russet Norkotah and Century Russet. This report summarizes the four-year project. '/ Superintendent/Professor and Biological Sciences Research Technician III, respectively. Klamath Experiment Station, Klamath Falls, OR. 2 / Klamath County Cooperative Extension Agent. Klamath Falls, OR. Acknowledgments: Funding for this project from the Oregon Potato Commission, and cooperation from several local potato growers is gratefully recognized. 42 Klamath Experiment Station 1997 Klamath Experiment Station Effect of Seedpiece Size on Performance of Three Potato Varieties, 1997 Procedures Commercial Seed Cutting Samples of 40 to 50 lb of cut seed were obtained from 18 commercial seed lots in the Klamath Basin in 1994 and 1995. Each lot was sorted to seedpiece sizes of <1.0 oz, 1.0 to 1.5 oz, 1.5 to 2.0 oz, 2.0 to 2.5 oz, and > 2.5 oz. The weight and number of seedpieces in each size fraction was recorded. Seed lots evaluated represented nearly equal numbers of mechanical and hand cutting operations and Russet Burbank and Russet Norkotah varieties. Potato Response to Seed Size Seed tubers of Russet Burbank, Russet Norkotah, and Century Russet varieties were obtained from local seed growers each year. All seed lots were generation H or generation III with very little virus content, based on winter greenhouse tests. Tubers were individually sized to 3, 5, 7, 9, and 11 oz, within a tolerance of one-quarter ounce, to obtain appropriate size from four-cut pieces to plant four replications of one-row plots with 3/4, 1-1/4, 1-3/4, 2-1/4, and 2-3/4 oz seedpieces. Russet Burbank plots were 30 hills at 12-inch seed spacing in 32-inch rows. Russet Norkotah and Century Russet plots were 42 hills at 8.7-inch seed spacing. Eight (Russet Burbank) or 11 (Russet Norkotah and Century Russet) tubers were used for each plot with two bud-end pieces discarded in each case to ensure unifoi iii numbers of bud-end and stem-end pieces for all plots. Each year, cut seed was treated with Tops 2.5 fungicide immediately after cutting and stored at approximately 55 °F and 95 percent relative humidity for about 10 days before planting to allow suberization. Plots were arranged in a split-plot design with variety as the main plot and seedpiece size as the split-plot. Potatoes were planted with a tworow, assisted-feed planter on June 13, 1995, May 24, 1996, and May 20, 1997. Standard cultural practices included fertilizer rates of 1,000 lb/acre of 15-15-15, banded at planting; weed control with standard rates of Eptam and Matrix and approximately 20 inches of irrigation water applied with solid-set sprinklers. Crop protection measures included fumigation with Telone II at 18 gpa, insect control with Di-syston, applied in the seed furrow at planting and two applications of Monitor, and fungal disease control with applications of Bravo, Ridomil-MZ, Mancozeb, and Kocide at labelled rates. Vines were desiccated with Diquat applied on September 26, 1995, September 6, 1996 and September 2, 1997. Potatoes were harvested with a one-row, diggerbagger on October 10, 1995, September 27, 1996, and September 26, 1997. All tubers from each plot were stored at about 55 °F until graded about three weeks after harvest. Emergence data were recorded at 22 days after planting each year and at least once more when all plants were emerged. Stem numbers were determined for 10 consecutive plants in each plot after vine desiccation. Tubers were graded to USDA grade standards. Specific gravity was determined by the weight-in-air, weight-in-water method using 10 lb samples of U.S. No. I s in the 8- to 12-oz size fraction. Ten large tubers from each plot were cut lengthwise to inspect for internal defects. Economic interpretations were based on the following assumptions: 1. Seed costs including cutting and treating Klamath Experiment Station 1997 43 Klamath Experiment Station Effect of Seedpiece Size on Performance of Three Potato Varieties, 1997 estimated to be $12/cwt. 2. Seed use based on 10-inch spacing (Russet Burbank) or 8-inch spacing (Russet Norkotah and Century Russet) in 36-inch rows with no misses or multiple drops. 3. U.S. No. ls valued at $5.00/cwt for 4-8 oz; $10.00/cwt for 8-12 oz; $8.00/cwt for > 12 oz. 4. U.S. No. 2s valued at $3.00/cwt. 5. Culls and Bs (< 4 oz) valued at $1.00/cwt. All data were subjected to analysis of variance using MSUSTAT software. Plant emergence data were analyzed using the arcsin transformation. Crop value data was calculated for each plot and was included in the statistical analysis. Analyses of data over years used a splitsplit-plot design with years as the main plot, variety as the split-plot, and seedpiece size as the split-split-plot. Results and Discussion Commercial Seed Cutting Six cut seed lots from commercial potato growers were examined in 1994, and 12 lots were evaluated in 1995 (Table 1). Seedpiece size distribution was highly variable within and between seed lots. One seed lot had an average weight of only 1.14 oz/seedpiece and 75 percent of seedpieces less than 1.5 oz on a number basis. At the other extreme, one lot had an average weight of 2.54 oz/seedpiece, less than 3 percent under 1.5 oz, and 78 percent greater than 2.0 oz/ seedpiece. Russet Burbank seed lots were cut slightly smaller than Russet Norkotah lots; however, this can be accounted for by the seed cutting method. Clearly, seed lots that were hand cut had fewer small seedpieces and more large pieces. Uniformity of size was not much better in the hand cut lots. Only three seed lots had 60 percent or 44 Klamath Experiment Station 1997 more pieces in the 1.5- to 2.5-oz size fractions. Three of the hand-cut seed lots had excessive numbers of seedpieces over 2.5 oz, while five machine-cut seed lots had over 50 percent of seedpieces less than 1.5 oz. Potato Response to Seed Size The response of Russet Burbank to seedpiece size in the 1994 study was very similar to results obtained in 1995-97, when three varieties were evaluated. The 1994 findings have been reported earlier and are not included in this analysis and report. Effects of seedpiece size on plant emergence were consistent between varieties and over years (Table 2). Russet Burbank emerged slightly earlier than the other varieties. In all varieties, emergence was delayed by small seedpieces. Final stands were over 95 percent in all cases except the 3/4 oz seed size for Century Russet in 1995 (90 percent) and 1997 (88 percent). Differences in canopy vigor were visually evident in all varieties through the first one-half of the vegetative growth phase. Plants from large seedpieces achieved row closure 10 days to 2 weeks earlier than plants from the smallest seedpieces. Stem numbers accounted for much of the differences in plant size. Stem numbers were slightly higher in Century Russet than other varieties and were higher for large seedpieces in each year (Table 2). Favorable weather throughout the 1997 growing season resulted in very high yields. Average total No. I yields of 453. 480, and 604 cwt/acre for Russet Burbank, Russet Norkotah, and Century Russet, respectively, were 42, 70 and 28 percent greater than means for the previous two years. The response to seedpiece Klamath Experiment Station Effect of Seedpiece Size on Performance of Three Potato Varieties, 1997 size was similar for all varieties. The interaction between variety and seedpiece size was not significant for any yield parameters in 1997. As in previous years, Century Russet experienced the largest yield response and Russet Norkotah was least responsive to seed size (Table 3). Total No. 1 yields were at least 80 cwt/acre higher for 1-3/4 oz than for 3/4 oz seedpieces in all varieties. Century Russet achieved maximum yield of No. ls at 1-3/4 oz seed size. Russet Burbank and Russet Norkotah produced maximum No. 1 yields at 2-3/4 oz. Seedpiece size greatly influenced tuber size distribution in all varieties. As seed size increased, yields of small No. is and Bs increased rapidly, while yield of No. ls > 12 oz declined. Effects of variety and seedpiece size on yields over three years are summarized in Table 4. The statistical significance of experimental variables and interactions are shown in Table 5. Yield differences between years were significant for all parameters except culls. Significance was found for all parameters between varieties. Russet Burbank produced similar yields of No. 1s, but higher yields of No. 2s and culls than Russet Norkotah. Century Russet achieved higher No. 1 yields in all size fractions. The variety-by-year interaction was also significant for all parameters. This waslargely due to a much greater difference in yields for Russet Norkotah than the other varieties over years. Russet Norkotah experienced very low yields and little response to seedpiece size in 1995. Earlier-than-normal vine death suggested some form of the early dying complex affected Russet Norkotah in 1995. In 1996 and 1997, the response of Russet Norkotah to seedpiece size was similar to the responses observed for Russet Burbank and Century Russet. Effects of seedpiece size on yields were significant for all parameters except yields of No. 2s and culls over years. Total No. 1 yields increased by 38 and 44 cwt/acre as seed size increased from 3/4 oz to 1-1/4 and 13/4 oz. Further increases in seedpiece size resulted in little yield increase but reduced tuber size. Differences in response to seedpiece size between varieties were not significant except for yield of 4- to 8-oz No. ls and total yield. Seedpiece size did not affect specific gravity or internal defects in any variety or year. Specific gravity was always measured in tubers within 8 to 12 oz. As seedpiece size altered tuber size distribution, there may have been minor affects on specific gravity because of changes in tuber size distribution. Large tubers were inspected for internal defects. Hollow heart and brown center were observed in less than 10 percent of tubers examined each year. Seedpiece size did not affect the incidence of defects in tubers examined. Evaluation of effects of seedpiece size on economics of potato production requires an assessment of increased seed costs for use of larger seedpieces. Based on the assumptions previously stated, seed costs are shown in Table 6. Seed costs are less for Russet Burbank because of lower plant populations used for this variety. The values assigned for yield components are the approximate values for fresh-market russets in the Klamath Basin in the late fall of 1997. Typical Klamath Basin Russet Norkotah crops were returning about $4.00/cwt to growers at these prices. - Klamath Experiment Station 1997 45 Klamath Experiment Station Effect of Seedpiece Size on Performance of Three Potato Varieties, 1997 Averaged over three years, the incremental increase in gross crop value for Russet Burbank was about $200/acre for seed size increases up to 2-1/4 oz (Table 6). Gross value declined by $200/acre for the last size increase. Russet Norkotah experienced increases of $150 and $70/acre for the first incremental increases in seedpiece size and little change among the three largest sizes. Century Russet had incremental increases in gross value of about $500, $300, and S 100/acre to the 2-1/4 oz size and no change for the final incremental increase in seed size. When crop values are adjusted for the change in seed costs associated with variable seedpiece size, the varieties responded differently. Russet Burbank achieved economically optimum production at 2-1/4 oz seed size. The increase in value from 3/4 to 2-1/4 oz was $480/acre. Russet Norkotah was not economically responsive. The largest seed size produced the lowest returns. Century Russet reached maximum returns at 1-3/4 oz seed size. The increase in value from 3/4 to 1-3/4 oz was $620/acre for Century Russet. Conclusions This study has confirmed the need for better quality control in seed-cutting operations. Significant economic benefits have been demonstrated for use of seedpieces in the range of 1-3/4 to 2-1/4 oz. Findings also show tuber size to meet specific market requirements can be manipulated by using smaller seedpieces when large tubers are desirable, and larger seedpieces when small tubers are at a premium. An example would be use of large seedpieces to produce higher yields of creamer size tubers for redskinned or specialty varieties. 46 Klamath Experiment Station 1997 Klamath Experiment Station Table 1. Seedpiece size distribution in 18 commercial cut seed lots evaluated at 'Klamath Falls, OR, in 1994 and 1995. Variety Cutting method Average weight < 1.0 oz 1.0-1.5 oz Seedpiece size 1.5-2.0 oz 2.0-2.5 oz > 2.5 oz % (number basis) OZ R. Burbank R. Burbank R. Burbank R. Burbank R. Burbank R. Burbank R. Burbank R. Burbank Machine Machine Machine Machine Machine Machine Hand Hand 1.50 1.14 1.53 2.05 1.56 1.64 2.32 1.65 16.5 46.0 19.9 3.9 13.2 24.7 0.0 8.7 29.7 28.8 30.2 17.3 34.4 24.3 5.1 30.4 26.1 17.2 33.0 29.0 35.9 23.3 24.0 40.9 19.5 7.0 14.8 29.8 13.9 13.8 39.8 16.6 8.2 1.0 2.1 20.0 2.6 14.4 31.1 3.4 R. Norkotah R. Norkotah R. Norkotah R. Norkotah R. Norkotah R. Norkotah R. Norkotah R. Norkotah R. Norkotah R. Norkotah Machine Machine Machine Machine Hand Hand Hand Hand Hand Hand 1.49 1.42 1.47 1.56 2.54 1.82 1.65 2.30 1.77 1.58 29.5 30.9 21.9 19.7 0.0 4.5 4.5 1.9 5.9 13.8 20.5 24.2 30.2 29.2 2.6 25.0 34.3 6.9 14.1 24.0 20.2 23.3 32.3 29.5 19.5 35.9 38.6 15.9 47.8 37.6 15.9 14.0 12.3 15.0 32.2 24.4 18.3 33.7 20.5 18.5 13.9 7.6 3.3 6.6 45.7 10.2 4.3 41.6 11.7 6.1 R. Burbank R. Norkotah Mean Mean 1.67 1.76 16.5 13.3 25.0 21.1 28.7 30.0 19.4 20.5 10.4 15.1 Mean Mean Machine Hand 1.54 1.95 22.6 4.9 26.8 17.8 27.0 32.5 15.6 25.5 8.0 19.3 Klamath Experiment Station 1997 47 Klamath Experiment Station Table 2. Effect of seedpiece size on percent emergence 22 days after planting and number of stems per plant for Russet Burbank, Russet Norkotah, and Century Russet potatoes grown at Klamath Falls, OR, 1995-97. Variety Seed size (oz) 1995 Emergence (%) 1996 1997 mean 1995 Stems/plant mean 1996 1997 Russet Burbank 3/4 1-1/4 1-3/4 2-1/4 2-3/4 82 97 96 96 100 91 94 100 98 99 44 52 59 60 61 72 81 85 85 87 1.90 2.45 2.65 - 3.05 3.23 2.05 2.45 2.55 3.20 3.45 1.58 1.85 2.18 2.58 2.58 1.84 2.25 2.46 2.94 3.09 Russet Norkotah 3/4 1-1/4 1-3/4 2-1/4 2-3/4 46 62 70 79 74 50 67 67 64 77 25 38 51 53 67 40 56 63 65 73 2.08 2.63 3.00 3.28 3.40 2.28 2.40 2.63 3.30 3.38 1.28 1.93 2.18 2.48 2.53 1.88 2.32 2.60 3.02 3.10 Century Russet 3/4 1-1/4 1-3/4 2-1/4 2-3/4 38 54 76 79 79 64 65 81 77 78 14 20 21 30 38 39 46 59 62 65 2.15 2.73 3.48 3.65 3.73 2.10 2.83 2.95 3.78 3.53 1.70 2.30 2.98 3.10 3.43 1.98 2.62 3.14 3.51 3.60 Variety main effect: Russet Burbank Russet Norkotah Century Russet 94 66 65 96 65 73 55 47 25 82 59 54 2.66 2.88 3.15 2.74 2.80 3.04 2.15 2.08 2.70 2.52 2.59 2.96 CV (%) LSD (P 0.05) 21 10 12 6 24 NS 18 10 18 0.42 20 0.40 26 0.46 13 0.13 55 71 81 85 84 68 75 83 80 85 28 37 44 48 55 50 61 69 71 75 2.04 2.60 3.04 3.33 3.45 2.14 2.56 2.71 3.43 3.45 1.52 2.03 2.45 2.72 2.85 1.90 2.40 2.73 3.16 3.25 10 6 8 5 19 7 18 7 11 0.27 12 0.30 12 0.22 13 0.17 Seed size effect: 3/4 1-1/4 1-3/4 2-1/4 2-3/4 CV (c7c) LSD (P = 0.05) 48 Klamath Experiment Station 1997 Klamath Experiment Station Table 3. Effect of seedpiece size on yield, grade, and tuber size distribution of Russet Burbank, Russet Norkotah, and Century Russet potatoes grown at Klamath Falls, OR, 1997. Variety Seed size 4 - 8 oz Yield U.S. No. is 8-12 oz > 12 oz total Bs Yield No. 2s culls total $/A cwt/A OZ Gross value R. Burbank 3/4 1-1/4 1-3/4 2-1/4 2-3/4 94 91 174 200 197 131 136 173 181 204 178 135 139 113 118 404 363 485 495 519 25 30 42 -57 62 76 59 81 63 65 42 42 60 37 39 547 494 668 651 686 3510 3150 4050 4000 4270 R. Norkotah 3/4 1-1/4 1-3/4 2-1/4 2-3/4 95 149 165 180 229 115 168 194 211 191 211 138 148 115 92 420 455 506 506 512 13 31 37 56 65 16 15 13 11 8 4 8 3 6 4 453 508 559 578 588 3370 3610 4020 4020 3880 Century R. 3/4 1-1/4 1-3/4 2-1/4 2-3/4 136 201 220 233 236 158 208 254 213 252 246 182 192 168 124 540 591 666 614 611 31 46 39 54 66 27 19 11 7 20 8 5 4 9 6 605 660 720 684 703 4340 4640 5250 4720 4820 151 164 205 165 176 217 137 141 182 453 480 604 43 40 47 69 13 17 44 5 7 609 537 674 3800 3780 4750 23 30 26 37 42 NS 20 81 37 NS 55 14 173 25 17 79 690 108 147 186 205 221 135 171 207 202 216 212 152 159 132 111 454 469 552 538 547 23 35 39 55 64 40 31 35 27 31 18 19 22 17 16 535 554 649 637 659 3740 3800 4440 4250 4320 17 25 18 27 26 33 11 46 27 10 34 10 82 NS 10 50 12 Variety main effect: R. Burbank R. Norkotah Century R. CV (%) LSD (P = 0.05) 22 Seed size effect s 3/4 1-1/4 1-3/4 2-1/4 2-3/4 CV (9-) LSD (P = 0.05) 390 Klamath Experiment Station 1997 49 Klamath Experiment Station Table 4. Mean effect of seedpiece size on yield, grade, and tuber size distribution over three years for Russet Burbank. Russet Norkotah, and Century Russet potatoes grown at Klamath Falls, OR, l 995-97. Variety Seed size 4-8 oz Yield U.S. No. ls 8-12 oz > 12 oz oz total Bs Yield No. 2s culls total cwt/A R. Burbank 3/4 1-1/4 1-3/4 2-1/4 2-3/4 93 129 162 185 178 112 126 144 145 146 93 81 79 82 56 298 336 385 412 380 30 37 . 48 62 70 42 34 38 38 36 32 23 25 20 36 401 431 495 533 522 R. Norkotah 3/4 1-1/4 1-3/4 2-1/4 2-3/4 100 133 148 156 172 110 130 135 141 139 112 82 82 67 64 321 345 363 363 376 20 38 45 52 60 10 11 10 10 8 10 11 7 10 3 361 403 426 435 448 Century R. 3/4 1-1/4 1-3/4 2-1/4 2-3/4 131 177 214 223 266 138 189 209 209 203 154 126 118 126 97 423 491 540 557 566 28 49 51 57 79 15 10 7 8 12 11 8 10 9 9 476 558 608 631 665 149 142 202 135 131 189 78 81 124 362 354 515 49 43 52 38 10 10 27 8 10 476 415 588 16 10 20 11 40 14 13 20 32 6 51 4 97 5 11 20 108 139 175 188 205 120 143 163 165 163 120 103 93 92 72 347 385 429 444 441 26 40 48 57 70 22 19 18 19 18 18 14 14 13 16 413 458 510 533 545 16 12 20 14 40 18 13 25 32 7 51 NS 97 NS 11 25 Variety main effect: R. Burbank R. Norkotah Century R. CV (%) LSD (P = 0 05) Seed size effect: 3/4 1-1/4 1-3/4 2-1/4 2-3/4 CV (%) LSD (P= 0.05) 50 Klamath Experiment Station 1997 Klamath Experiment Station Table 5. Statistical summary of effects of seedpiece size, three varieties, and year on yield components and gross crop value of potatoes grown at Klamath Falls, OR, 1995-97. Yield U.S. No. Is 8-12 oz > 12 oz Factor 4-8 oz Year ** ** Variety ** ** Year x Variety ** Size ** Year x Size total Gross value NS ** ** ** ** ** ** ** ** ** ** ** NS NS ** ** ** NS NS NS NS NS NS NS NS NS NS NS Yield No. 2s culls total Bs ** ** •* ** ** ** ** ** ** ** ** ** NS ** ** Variety x Size ** NS NS Year x Variety x Size ** NS NS NS NS: not significant. * Significant at P= 0.05. ** Significant at P= 0.01. Klamath Experiment Station 1997 51 Klamath Experiment Station Table 6. Effect of seedpiece size on gross crop value, seed costs, and gross value less seed costs for Russet Burbank. Russet Norkotah, and Century Russet potatoes grown at Klamath Falls, OR, 1995-97. Variety Seed size Gross crop value 1995 1996 1997 mean Seed cost Gross value less seed cost 1995 1996 1997 mean $/A OZ Russet Burbank 3/4 1-1/4 1-3/4 2-1/4 2-3/4 1910 2240 2590 3070 2390 2080 2770 2530 2570 2370 3510 3150 4050 4000 4270 2500 2720 3060 3210 3010 120 200 280 350 430 1790 2040 2310 2720 1960 1960 2570 2250 2220 1940 3390 2950 3770 3650 3840 2380 2520 2780 2860 2580 Russet Norkotah 3/4 1-1/4 1-3/4 2-1/4 2-3/4 1770 1760 1610 1960 1880 2510 2730 2820 2450 2810 3370 3610 4020 4020 3880 2550 2700 2820 2810 2860 150 250 340 440 540 1 620 1510 1270 1520 1340 2360 2480 2480 2010 2270 3220 3360 3680 3580 3340 2400 2450 2480 2370 2320 Century Russet 3/4 1-1/4 1-3/4 2-1/4 2-3/4 2940 3990 4050 4900 4650 2760 2960 3160 3210 3300 4340 4640 5250 4720 4820 3350 3860 4180 4280 4260 150 250 340 440 540 2790 3740 3710 4460 4110 2610 2710 2820 2770 2760 4190 4390 4910 4280 4280 3200 3610 3840 3840 3720 Mean 3/4 1-1/4 1-3/4 2-1/4 2-3/4 2210 2660 2780 3310 2970 2450 2820 2840 2740 2830 3740 3800 4440 4250 4320 2800 3090 3350 3430 3370 NC NC NC NC NC NC NC NC NC NC NC NC NC NC NC NC NC NC NC NC NC NC NC NC NC 340 310 390 210 NC NC NC NC NC LSD (P = 0.05) NC: Not calculated. 52 Klamath Experiment Station 1997
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