Klamath Experiment Station Potato Variety Screening Trials, 1997 K.A. Rykbost and J. Maxwell' A bstract The Oregon Potato Variety Development Program evaluates approximately 80,000 single-hill selections annually from breeding programs in Idaho, Colorado, and North Dakota. The Klamath Experiment Station (KES) participates in the overall effort by conducting replicated yield trials at the preliminary, statewide, and western regional levels. In 1997, two additional trials were conducted to evaluate Russet Norkotah, Russet Burbank, and Shepody clones developed at Oregon State University through transgenic techniques. Over 125 selections were included in these trials at KES in 1997. Two Oregon selections will be released in early 1998. Umatilla Russet (A082611-7) is a processing russet that has performed very well for French fry production in the Columbia Basin of Oregon and Washington. Over 400 acres of A082611-7 seed were certified in 1997. Russet Legend (CO083008-1) has performed well for French fry production in the Treasure Valley of Eastern Malheur County. Oregon, and Southwestern Idaho, and for fresh market in Eastern Idaho. Over 160 acres of CO083008-1 seed passed certification in 1997. Several other Oregon selections in advanced stages of evaluation appear to be candidates for release. Three russet-skinned selections and two round-white selections from the statewide trial will be ad- vanced to regional trials in 1998. Most of the entries in the 1997 statewide trial exceeded the standard varieties in yield, quality, or both. Several of the OSU transgenic clones are resistant to potato virus Y and were not significantly different than standard varieties in yield or quality. The transgenic selections will be considered in a separate report. Introduction Considerable progress has been made in the past 10 years improving potato varieties. Russet Burbank, the long-time industry standard for fresh market and French fry production, requires high inputs and is vulnerable to several physiological disorders. It is no longer the dominant variety in the Klamath Basin or the Treasure Valley. New varieties have also captured a significant portion of the Columbia Basin acreage; however, the newer varieties replacing Russet Burbank also have weaknesses. Traditional breeding programs continue to produce selections with superior quality for specific markets. Recently, through genetic engineering techniques, standard varieties have been altered to incorporate disease or pest resistance or to improve other characteristics. A third area being pursued is line selections within a variety. Examples from all of these methods were included in material evaluated in the Oregon statewide program in '/ Superintendent/Professor and Biological Sciences Research Technician III, respectively, Klamath Experiment Station. Klamath Falls, OR. Acknowledgments: Financial support for this program from the Oregon Potato Commission; the Cooperative State Research. Education, and Extension Service (CSREES); and the USDA Agricultural Research Service (ARS) is gratefully recognized. 12 Klamath Experiment Station 1997 Klamath Experiment Station Potato Variety Screening Trials, 1997 1997. This report summarizes the performance of selections in the Oregon and regional programs at Klamath Falls. Procedures All trials were conducted on a Poe fine sandy loam soil in a 2-year rotation with spring cereals. Nematodes and related diseases were controlled with 18 gallons per acre (gpa) of soil fumigant Telone II applied at about 16-inch depth on 18-inch centers with a ripper on October 22, 1996. All seed lots were hand cut to approximately 1.5 to 2.5 ounces (oz) per seedpiece, treated with thiophanate-methyl (Tops 2.5), at 1.0 pound per hundred pounds (lb/cwt), and suberized at about 55 °F and 95 percent relative humidity for 10 to 14 days before planting. Potatoes were planted at 8.7-inch spacing in 32-inch rows with an assisted-feed, two-row planter in all trials. Insecticide Disyston was applied in the seed furrow at 3.0 lb active ingredient (a.i.)/acre at planting. Fertilizer was banded on both sides of rows at 1000 lb/acre of 16-8-8-14. Weeds were controlled with Eptam applied at 3.0 lb a.i./ acre with a conventional ground sprayer and incorporated immediately with a rolling cultivator on May 28, and Matrix applied at 1.5 oz (0.0234 lb a.i.)/acre on June 23 and incorporated with 0.5 inches of irrigation water. Irrigation was applied twice weekly with solid-set sprinklers arranged on 40-foot by 48-foot spacing. Total crop water, including irrigation and rainfall, was approximately 24 inches. All trials were planted on May 19. The preliminary-yield trial included 5 standard varieties and 67 numbered selections in 20-hill plots with 2 replications. Five standard varieties and 19 numbered selections were included in the statewide trial. The regional trial included 3 standard varieties, 10 numbered selections, and 5 line selections of Russet Norkotah. All trials were arranged in a randomized complete block design and, except in the preliminary trial, included 30hills/plot with 4 replications. Fungicides, applied aerially, included Bravo at 1.0 lb a.i./acre on July 5, Ridomil-MZ at 1.5 lb a.i./acre on July 17, Kocide at 2.0 lb a.i./ acre on August 9, and Bravo at 2.0 lb a.i./acre on August 29. Monitor was applied with the fungicides at 0.75 lb a.i./acre on July 17 and August 29. Vines were desiccated with Diquat applied with a ground sprayer at 1.0 pint/acre on September 2 and September 7 for the western regional trial and September 7 and September 11 for preliminary and statewide trials. Kocide was _ . applied with the Diquat at 2.0 lb a.i./acre on September 7. Vines were shredded with a rotobeater one day before harvest. Potatoes were harvested with a one-row digger-bagger on September 29 (western regional), September 30 (preliminary trial), and October 1 (statewide trial). All tubers from each plot were stored at about 55 °F and 95 percent relative humidity until tubers were graded in late October. External tuber characteristics were noted for each replication during grading. Ten large (usually over 10 oz) tubers from each plot were cut lengthwise and inspected for internal defects. Air and water weights were used to determine specific gravity on samples of approximately 10 lb of U.S. No. ls in the 8- to 12-oz size. USDA grade standards were followed to separate B size (< 4 oz), U.S. No. ls (4 to 12 oz and > 12 oz), U.S. No. 2s, and culls Klamath Experiment Station 1997 13 Klamath Experiment Station Potato Variety Screening Trials, 1997 categories. U.S. No. 1 yields were not adjusted for external blemishes such as rhizoctonia or scab or for internal defects such as hollow heart or brown center. Samples of 6- to 12-oz tubers were saved from one replication of each selection in all trials to evaluate culinary quality. French fry tests were conducted to determine fry color on entries in preliminary, statewide, and western regional trials. Samples from the western regional and statewide trials were also evaluated for boiling, oven baking, and microwave preparation methods. The culinary tests were designed to detect serious deficiencies such as severe sloughing, off- flavors, aftercooking darkening, or poor texture. Tests were conducted in early November. Because the preliminary trial only included two replications, data were not subjected to statistical analyses. In other trials, yield, grade, and specific gravity data were statistically analyzed using MSTAT software. Only a portion of the data collected is presented in this report. Decisions on the disposition of selections at all levels of evaluation are based on data accumulated at all trial locations. Preliminary and statewide trial data from four Oregon trial sites (Hermiston, Ontario, Powell Butte, and Klamath Falls) are compiled and reviewed by members of the Oregon Variety Development Committee. Cooperators at Aberdeen, Idaho compile tri-state and western regional trial data, which is reviewed at annual committee meetings attended by up to 30 cooperators and industry representatives. Results and Discussion The 1997 growing season was generally very favorable for the potato crop at Klamath Falls. The only frost affecting trials occurred on 14 Klamath Experiment Station 1997 June 22 when plants were just emerging. The crop was protected with sprinkler irrigation and no damage to emerged plants was observed. Plants of many of the selections were unusually vigorous. Vines were difficult to desiccate at season-end. Yields were high to very high in many selections. Tuber size was larger than usual and selections with a tendency for malformations exhibited knobs, growth cracks, and skin abnormalities. For example, Russet Burbank had over 20 percent No.2s and culls in the preliminary, statewide, and regional trials. In each of these trials, Russet Burbank ranked very low in yield of No.ls. Preliminary Yield Trial Most of the entries in this trial were selections from crosses made at Aberdeen, Idaho, in 1992 and 1993. Several entries had excessive vine growth, and immature skins at harvest which resulted in serious skinning damage. Yields were generally very high but several of the selections, including Russet Burbank and Shepody, had many off-type, rough, or growthcracked tubers. Russet Burbank ranked 26th in total yield but only 60th out of 72 entries in No. I yield. Russet Norkotah produced 115 cwt/acre less total yield, but 33 cwt/acre more No. 1 yield than Russet Burbank. The conditions of this trial provided a good opportunity to detect weaknesses in tuber type and uniformity. Similar conditions and results were experienced at Powell Butte, the other short-season trial location. After reviewing data from all locations, 11 clones were selected for further evaluation (Table 1). All of these are russet-skinned lines. Several selections had adequate dry matter content and fry color for processing as French fries. Ranking in No. 1 yields across four locations ranged from Klamath Experiment Station Potato Variety Screening Trials, 1997 1st for A093317-5, which produced the highest No. I yield at three locations, to 32nd for A092281-3. None of the clones saved were equal to Russet Norkotah in tuber type and appearance. Entries selected from this trial were advanced to the statewide trial. Statewide Trial Crop development in this trial was similar to conditions experienced in the preliminary trial. Most selections had vigorous vine growth at the time of top killing (Table 2). All selections had later vine maturity than Russet Norkotah. Hollow heart incidence was quite high in Atlantic and three numbered selections, although in most cases the tubers involved were very large, particularly in A085165-1. The performance of standard varieties was similar to results observed in the preliminary trial. Russet Burbank produced a high total yield, but it had many No. 2s and culls (Table 3). Although Russet Norkotah had 130 cwt/acre less total yield than Russet Burbank, No. 1 yield was 45 cwt/acre higher. At KES and across all locations, Russet Burbank ranked 23rd out of 24 entries in yield of No. ls. The overall quality and performance of entries in this trial was very good. Most of the Russet-skinned selections were attractive in appearance, had relatively good tuber type, produced high yields, and had fewer external and internal defects than Russet Burbank. The two round white chipping selections, A091812-1 and A091812-2, ranked 1st and 2nd, respectively, in No. 1 yields across all locations. Only three selections from this trial were discarded from the program. The official release of A082611-7 as Umatilla Russet and C0083008-1 as Russet Legend is planned for early 1998. Both selections have good processing quality. Russet Legend is considered acceptable for fresh market by growers in Eastern Idaho. A085165-1, an attractive fresh market russet, completed three years of evaluation in the regional trial in 1996, was increased in greenhouse culture in 1997, and will be supplied to seed growers for rapid increase and commercial evaluation. It resembles Russet Norkotah, which was the male parent, in appearance, and typically exceeds Norkotah in No. 1 yield by 100 cwt/acre or more. The russet selection A087277-6 was included in the western regional trial for the first time in 1997. It will remain in the 1998 regional trial as a dual purpose line, suitable for processing and fresh market use. Another dual purpose russet, A089128-4, was included in the 1997 tristate trial and will advance to the regional trial in 1998. A090014-1 and A088103-3 are being advanced to the tri-state trial and are considered dual purpose, attractive russets. A090319-1 was probably the most attractive russet-skinned selection in this trial. It has very nice yellow flesh color. This selection will be entered in the regional specialty variety trial in 1998. It produced lower yields than most other clones in both 1996 and 1997. The chipping selections, A091812-1 and A091812-2, will advance to the regional chipping trial in 1998. Both lines produce very high yields. Late vine maturity and attached stolons were noted in both selections at KES in 1997. Six additional selections will be retained in the statewide trial in 1998 (Table 3). Western Regional Trial Three Texas and two Colorado line selections of Russet Norkotah were included in Klamath Experiment Station 1997 15 Klamath Experiment Station Potato Variety Screening Trials, 1997 this trial. All had later vine maturity than the standard Russet Norkotah entry (Table 4). The Colorado selections CORN-3 and CORN-8 and the Texas selection TXNS 223 were entered in the regional trial for the first time in 1997. In 1996 trials, TXNS 112 and TXNS 278 were only slightly later in vine maturity than the standard Russet Norkotah across seven locations. The 1997 KES data shows much later vine maturity for CORN-3 and TXNS 278. All Norkotah line selections were equal to or higher than the standard Russet Norkotah in specific gravity. CORN-3 had a high incidence of hollow heart. This selection produced very large tubers compared to the other Russet Norkotah selections. The relative yields of Russet Burbank and Russet Norkotah standards were the same as observed in preliminary and statewide trials (Table 5). Russet Burbank had the lowest yield of No. ls and the highest yield of No. 2s and culls of all entries. CORN-3 achieved the highest No. I yield, but tuber size was excessive. CORN-3 and CORN-8 produced significantly higher No. 1 yields than the standard Russet Norkotah. TXNS 278 was the lowest yielding Norkotah line selection. A8495-1 was not an official trial entry in 1997. It has completed three years of evaluation in the regional trial and is being evaluated commercially. This selection has looked very promising as a fresh market selection for the Klamath Basin. It has not produced high yields in KES trials but has very attractive tubers and excellent culinary quality. In this trial, A8495-1 exceeded Russet Burbank in No. 1 yields by 52 cwt/acre. All other selections produced higher No. 1 yields than A8495- l in the 1997 KES regional trial. Three entries; A82360-7, C085026-4, and TXAV657-27Ru, have completed three 16 Klamath Experiment Station 1997 years in regional trials. Two selections, A8792-1 and C087009-4, were discarded. All other selections in the 1997 trial will be continued in the program. The Colorado and Texas strain selections of Russet Norkotah have undergone exten- _ si ve seed increase and will constitute a significant portion of 1998 acreage in these states. Averaged over nine regional trial sites in 1997, all of these strains produced higher No. 1 yields than the standard Norkotah by 20 to 80 cwt/acre. Conclusions The quality of advanced selections in the Oregon and regional potato variety development programs is excellent. Oregon will formalize releases for Russet Legend and Umatilla Russet in 1998. Idaho plans to release a round white chipping selection, ND01496-1, which was selected from the Oregon program; a red-skinned selection, A82705-1R as Idarose; and two russeted selections, A81473-2 and A8495-1, which will be named Classic Russet, in 1998. Colorado plans to release a russet selection, AC83064-6, and a red-skinned selection, DT6063-1R in 1998. Releases planned for the near future in Texas include a round white chipping selection, ATX85404-8, and a russet selection, TXAV657-27Ru. Texas and Colorado continue moving forward with development of Russet Norkotah strain selections. Based on evaluation of the russet skinned selections at KES, A8495-1 may be the best candidate as a fresh market russet for the local industry. Klamath Experiment Station Table 1. Tuber yield and specific gravity of entries selected from the Preliminary Yield Trial for further evaluation, Klamath Falls, OR, 1997. Variety/ selection Yield U.S. No. ls 4-12 oz > 12 oz total Bs Yield No. 2s culls total Specific gravity cwt/A Russet Burbank Ranger Russet Shepody Russet Norkotah Atlantic 332 307 159 283 397 59 180 279 141 121 391 487 438 424 518 77 55 17 39 92 28 51 0 14 0 102 13 65 7 22 598 606 519 483 632 1.085 1.086 1.082 1.069 1.095 C0093031-3 A092130-2 A092246-3 AO92252-1 A092260-8 303 389 435 295 408 337 196 130 344 150 640 585 565 639 558 24 52 68 33 59 0 0 6 23 15 5 30 0 6 36 669 667 639 701 668 1.080 1.083 1.081 1.086 1.083 A092270-4 A092281-3 A092303-3 A092304-1 A092378-1 A093317-5 333 438 391 347 264 488 119 101 127 46 195 183 452 539 518 393 459 671 50 39 34 103 33 42 0 12 7 4 0 0 4 2 72 14 23 10 506 592 631 514 515 723 1.088 1.090 1.100 1.082 1.082 1.083 Mean' 348 169 517 51 10 26 604 1.085 1/ Mean for standard varieties and clones selected only. Klamath Experiment Station 1997 17 Klamath Experiment Station Table 2. Characteristics of entries in the Oregon Statewide Trial, Klamath Falls, OR, 1997. Variety/ Percent Vigor Vine Specific selection stand rating' maturity2 gravity Russet Burbank Ranger Russet Shepody Russet Norkotah Atlantic 93 92 93 93 87 3.5 4.0 3.0 3.3 3.8 3.3 3.0 3.5 2.0 3.3 1.088 1.079 1.075 1.069 1-.092 8 0 5 8 20 A082611-7 C0083008-1 A085165-1 A087277-6 A089128-4 95 98 96 96 98 3.0 2.8 2.3 3.5 3.5 3.3 3.8 3.5 3.8 3.5 1.084 1.087 1.078 1.085 1.094 5 11 18 3 3 A090014-1 C0090319-1 A088103-3 A091522-4 A091812-1 95 99 98 96 96 3.5 2.8 3.3 2.3 3.0 3.5 4.0 4.3 3.8 4.0 1.085 1.082 1.081 1.079 1.086 0 3 18 5 3 A091812-2 A092007-2 A092016-2 A092016-3 A092017-6 97 96 94 96 98 4.3 3.5 2.5 2.5 2.8 3.8 4.0 3.0 3.5 4.3 1.085 1.084 1.076 1.079 1.083 3 3 0 3 0 A092019-13 A092023-3 A092056-7 A092173-2 97 96 94 92 2.0 3.5 2.0 3.3 4.3 3.0 3.8 4.5 1.084 1.08 1.081 1.079 8 5 0 35 Mean LSD (P = 0.05) 95 3.1 3.6 1.082 0.005 7 1 / Vigor rating: 1 a small, weak plant to 5, a large, robust plant. 2 / Vine maturity: 1 for early to 5 for late. 3 / H.H. & B.C.: hollow heart plus brown center in 10 large tubers/sample. 18 Klamath Experiment Station 1997 Percent H.H. & B.C.' Klamath Experiment Station Table 3. Tuber yield by grade for entries in the Oregon Statewide Trial, Klamath Falls, OR, 1997. Variety/ Selection Yield U.S. No. is 4-12 oz > 12 oz total Bs Yield No. 2s culls total cwt/A Russet Burbank Ranger Russet Shepody Russet Norkotah Atlantic 240 255 204 284 359 89 152 185 90 131 329 407 389 374 490 61 29 18 44 37 57 17 49 4 17 108 8 27 4 21 555 461 482 425 565 A082611-7 00083008-1* A085165-1* A087277-6' A089128-4* 300 217 284 365 372 122 262 253 148 115 421 479 537 513 487 60 15 28 42 38 25 14 15 12 44 18 12 8 11 12 523 520 588 578 580 A090014-1* A090319-1* A088103-3* A091522-4 A091812-1* 310 323 358 295 422 121 87 147 318 172 431 410 505 613 594 50 49 50 19 49 14 5 19 10 15 3 3 13 21 5 498 467 587 663 663 A091812-2* A092007-2' A092016-2 A092016-3* A092017-6* 502 318 299 367 256 159 l 32 113 70 248 661 450 412 437 504 61 35 42 46 37 9 13 39 14 19 14 5 12 15 6 745 502 505 512 564 A092019-13' A092023-3* A092056-7 A092173-2' 357 348 264 280 91 118 41 230 447 466 305 510 32 42 40 30 21 38 15 52 21 8 3 30 520 553 363 622 Mean CV (%) LSD (P= 0.05) 316 18 82 150 36 76 465 17 113 40 36 20 22 82 26 16 88 20 543 15 112 * Retained for further evaluation Klamath Experiment Station 1997 19 Klamath Experiment Station Table 4. Characteristics of entries in the Western Regional Trial, Klamath Falls, OR, 1997. Variety/ Percent Vigor Vine Specific selection stand rating maturity2 gravity H.H. & B.C. ; 98 98 93 97 98 3.5 3.8 2.3 2.8 3.8 4.3 3.8 2.3 4.5 4.5 1.084 1.085 1.070 1.086 1.090 l I 8 8 0 20 96 92 100 89 98 3.5 3.3 2.0 4.8 3.3 3.3 3.5 3.0 3.3 4.0 1.094 1.090 1.082 1.087 1.076 8 0 0 26 23 CORN-8 NDD840-1 TX1385-I2RU TXAV657-27RU TXNS 112 99 97 99 96 96 2.5 2.0 3.0 3.3 3.8 2.8 4.3 2.8 3.5 3.0 1.074 1.073 1.076 1.074 1.069 8 23 8 3 13 TXNS 2 23 TXNS 278 A8495-1 98 98 98 3.5 2.5 1.0 3.5 4.0 3.5 1.071 1.070 1.082 13 8 3 Mean LSD (P = 0.05) 97 3.0 3.6 1.079 10 Russet Burbank Ranger Russet Russet Norkotah A82360-7 A8792-I AC87084-3 A087277-6 C085026-4 C0087009-4 CORN-3 0.006 1/ Vigor rating: l is a small. weak plant to 5, a large, robust plant. 2/ Vine maturity: 1 for early to 5 for late. 3 / H.H. & B.C.: hollow heart plus brown center in 10 large tubers/sample. 20 Klamath Experiment Station 1997 Percent Klamath Experiment Station Table 5. Tuber yield by grade for entries in the Western Regional Trial, Klamath Falls, OR, 1997. Variety/ selection Yield U.S. No. ls 4-12 oz > 12 oz total Yield No. 2s culls total cwt/A Russet Burbank Ranger Russet Russet Norkotah A82360-7 A8792-] 284 349 261 347 290 56 155 164 165 224 339 504 425 512 513 75 63 41 46 35 83 58 5 34 33 63 11 0 73 83 560 635 471 663 663 AC87084-3 A087277-6 C085026-4 C0087009-4 CORN-3 398 345 264 364 326 152 167 192 53 256 550 512 456 418 582 46 45 30 102 34 19 32 24 35 30 3 7 2 7 12 617 596 512 561 658 CORN-8 NDD840-1 TX1385-12RU TXAV657-27RU TXNS112 296 267 299 283 347 254 152 176 216 150 550 419 475 499 497 35 61 52 32 53 31 36 26 41 24 4 1 8 21 8 619 517 560 593 581 TXNS 223 TXNS 278 A8495-1 . 401 304 263 86 120 129 487 424 391 58 61 34 10 10 8 5 1 9 561 496 442 Mean CV (7c ) LSD (P= 0.05) 316 17 74 159 39 89 475 16 109 50 35 25 30 74 31 18 84 21 572 14 111 Klamath Experiment Station 1997 21
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