Molecular Biology Lab.
Hung, Chih-Hsin
Associate Professor
Phone: +886-7-6577711 ext 3414
Fax: +886-7-6578945
E-mail: [email protected]
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Multidrug-Resistant Microbial Infection and Therapy Study
Outbreak of multidrug resistance bacterium infections has become the
major trouble problem among the human being, livestock farm and fish farm.
Therapeutic methods by using antibiotics are difficult to control these microbial
infections. Multiple drug-resistant bacterium of Acinetobacter baumannii,
Pseudomonas aeruginosa, Klebsiella pneumonia, Staphylococcus aureus cause
serious nosocomial infections in intensive care units in every area of the world.
New drugs discovery is more delay speed than the bacterial mutation rate,
therefore the new therapeutic processes develop is emergent.
The
therapeutic methods of phage therapy is an old one, because of the antibiotics
resistant of bacterium are increased and the old method is remind again. In our
laboratory have prepared many obligate virulent phages for combating these
multi-drug resistant bacterium. Besides phage, we also study the lysin proteins,
bacteriocin and compound extraction from herbs to investigate the proper
agent for treating bacterium infections.
Biomarker Investigation for Osteoarthritis Diagnosis
Articular cartilage degeneration is a complex heterogenous disease. The
imbalance in cartilage synthesis and degradation is major to the development of
articular cartilage degeneration. The diagnosis of OA is generally base on
clinical and radiographic change. Other form of imaging used to measure
cartilage tissue directly is MRI (Magnetic resonance imaging), however, the
limitation of the radiographs for detecting cartilage destruction is that
significant cartilage degradation has occurred. Some biomarkers derived from
bone, cartilage and synovium are selected to screen the articular cartilage
degenerative progression, but most are major in detecting the specimens from
serum or synovial fluid. To avoid collecting the specimens by invasion to
patients’ bodies, we will develop the rapid detection system to exam the urinary
samples from articular cartilage degenerative patients.
IIm
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Protein 2-D system equipments (Bio-Rad x1),
SDS-PAGE and protein transfer equipments (Bio-Rad x3),
Ultra centrifuge (Hitachi x1),
PCR machine (x3),
agarose gel electroporesis tank (many),
Low-temperature and high-speed centrifuge (Kubota 3780x2),
High speed centrifuge (Kubota 3300Tx1),
CCD digital camera for DNA gel (x1),
Autoclave (x3),
Sonicator (x1),
Incubator (x3),
Oven (x1),
Chemical hood (x1),
Protein purification system (x1),
Laminar flow (P2 level) (Length 120 cm x1, 150 cmx1),
Whole wave spectrophotometer (x1),
4oC Refrigerator (2 door x1, 3 doorx3),
-20 oC Refrigerator (x2),
-80 oC Refrigerator (x1),
Eletroporator (1 for cell, 1 for bacterium),
Dark room (x1)
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Ph.D. program: 1 person
Graduate student: 3 persons
Undergraduate student: 4 persons
SSeelleecctteedd ppaappeerrss ((ffrroom
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1. Shen G. H., J. L. Wang, F. S. Wen, K. M. Chang, C. F. Kuo, C. H. Lin, H. R. Luo and C. H. Hung*
(2012) Isolation and Characterization of φkm18p, a Novel Lytic Phage with Therapeutic
Potential Against Extensively Drug Resistant Acinetobacter baumannii. PLoS ONE (Accepted).
2. Hung C. H., N Tsao, Y. F. Zeng, S. L. Lu, C. N. Chuan, Y. S. Lin, J. J. Wu and C. F. Kuo. (2012)
Synergistic effects of streptolysin S and streptococcal pyrogenic exotoxin B on the mouse
model of group A streptococcal infection, Med Microbiol Immunol. 201:357-369.
3. Hung C. H., C. F. Kuo,C.-H. Wang, C. M.Wu, and N. Tsao. (2011) Experimental Phage Therapy
4.
5.
6.
7.
in Treating Klebsiella pneumoniae-Mediated Liver Abscesses and Bacteremia in Mice.
Antimicrob. Agents Chemother. 55:1358-1365.
Wang S. H., C. Y. Shen, T. C. Weng, P. H. Lin, J. J. Yang, I. F. Chen, S. M. Kuo, S. J. Chang, Y. K Tu,
Y. H. Kao and C. H. Hung* (2010) Detection of Cartilage Oligomeric Matrix Protein Using a
Quartz Crystal Microbalance. Sensors 10:11633-11643.
Wang C. P., W. C. Hung, T. H. Yu, C. A. Chiu, L. F. Lu, F. M. Chung, C. H. Hung, S. J. Shin, H. J.
Chen, Y. J. Lee. (2010) Genetic variation in the G-50T polymorphism of the cytochrome P450
epoxygenase CYP2J2 gene and the risk of younger onset type 2 diabetes among Chinese
population: potential interaction with body mass index and family history. Exp. Clin.
Endocrinol. Diabetes 118:346-52.
Shen, G. H., C. H. Hung, S. T. Hu, B. D. Wu, C. F. Lin, C. H. Chen, K. M. Wu, and J. H. Chen.(2009)
Combining PCR-Restriction Enzyme Analysis with Phenotypic Characters for Mycobacteria
Identification in Taiwan. Int. J. Tuberc. Lung Dis. 13:472-479..
Shen G. H., C. H. Chen, C. H. Hung, K. M. Wu, Y. Y. Liu, M. Y. Chen, J. H. Chen. (2009)
Combining the Capilia TB assay with smear morphology for rapid identification of
Mycobacterium tuberculosis complex. Int. J. Tuberc. Lung Dis.13:371-376.
(Update on 2012 / 10/ 01)
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