Physics
MONDAY, APRIL 6TH
Dr.AdamAbate
UniversityofCalifornia,SanFrancisco
4:00PMinTH411
Refreshmentsservedat3:50PM
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Droplet-based microfluidics for ultrahigh-throughput
single-cell biology
Abstract:
Mylabdevelopsmethodsforanalyzing,sorting,andengineeringsinglecellsusingdropletbasedmicro luidics.Inthispresentation,Iwilldescribewaysinwhichweareusing
thistoevolvenewenzymesand,bycombiningitwithnext-generationsequencing(NGS),
tomodelenzymesequence-functionrelationships.Byapplyingthistechniquetocell-free
extracts and automating with robotics, we are performing in vitro automated, continuousevolutiontoengineernewbiosyntheticpathways.Usingrelatedtechniques,wehave
developedanewmethodforsortingcellswecallPCR-ActivatedCellSorting(PACS)that
allowsenrichmentofrarecellsoutofaheterogeneouspopulationbasedoninfectionby
speci icviruses,theexpressionofspeci icgenes,orthepresenceofuniquemicroRNAs.
BypairingPACSwithsequencingofcellulargenomesandtranscriptomes,wecancorrelatethepresenceofthetargetsequenceswithhostcellphenotypes.Weareapplyingthis
to intratumor heterogeneity and latency of HIV infection in patients undergoing antiretroviral therapy. All of these projects leverage the unparalleled ability of dropletbasedmicro luidicsforperformingmillionsofsinglecellreactions.