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Technical Data Sheet 910
Page 1 of 1
Warthin-Starry Stain Kit
Description
Identifies Spirochetes and some other bacteria such as H. pylori and two causative agents of cat scratch disease, Bartonella henselae and Afipia felis.
Warthin-Starry Stain relies on the ability of certain bacteria to bind silver ions from solution. Subsequent addition of a reducing agent then converts
this bound silver to visible metallic silver. In the Warthin-Starry stain, tissue is sensitized prior to application of the silver complex. An aqueous silver
nitrate solution combined with the reducing agent, hydroquinone, is applied and a silver diamine complex is generated.
Staining Procedure
1. Attach slides to automatic stainer, deparaffinize, hydrate to water.
2. Rinse in distilled water, 2 changes.
3. Place slides in 1% silver nitrate solution for 45 seconds in microwave. Let stand for 1 minute at room temperature.
(Note: alternatively, in a laboratory microwave, heat at 80% power, 60ºC for 5 minutes, no standing time required).
4. Use 5 gm of Gelatin, provided, to 100 ml of distilled water to make a 5% gelatin solution. Stir until dissolved. Use in step 5 for the developer
solution. Store remainer at room temperature until needed again.
5. Preheat for 45 seconds in microwave in separate flasks: 2% silver nitrate, 5% gelatin, 0.15% hydroquinone. Preheat empty flask in microwave
with these.
6. Mix developer solution in order given: Use warm empty flask:
2% silver nitrate 1.5 ml
5% gelatin 3.75 ml
0.15% hydroqinone 2.0 ml
(Note: Prepared developer solution (7.25ml) can test approximately 48 slides - using 150µl of developer solution per slide)
7. When step 5 is completed, remove slides from silver solution. Do not rinse. Place slides horizontally on a slide rack and cover with developer.
Allow sections to develop until they are light yellow to golden brown, approximately 1 minute or less. (Note: developing step can also be carried
out in a laboratory microwave at 80% power set for 60ºC for 20 seconds).
8. Rinse thoroughly in 50 ml tap water which is preheated to approximately 56ºC in the microwave (450 watts) for 45 seconds.
9. Rinse in distilled water.
10. Attach to automatic stainer, dehydrate and clear. Mount in a xylene soluble mounting medium.
Results
Spirochetes - Black
Background - pale yellow to light brown
Adapted for the Jefferson Regional Medical Center Histo-Path Laboratory
Cat #.DescriptionSize
25093-1
Warthin-Starry Stain Kit
1 kit
To ORDER
In The U.S. Call:
In The U.S. Fax:
1(800) 523-2575 • (215) 343-6484
1(800) 343-3291 • (215) 343-0214
In Germany Call:
In Germany Fax:
+(49) 6221-765767
+(49) 6221-764620
In Asia Call:
In Asia Fax:
(886) 2 8712 0600
(886) 2 8712 2677
Order online anytime at www.polysciences.com
Should any of our materials fail to perform to our specifications, we will be pleased to provide replacements or return the purchase price. We solicit your inquiries concerning all needs for life sciences work. The information given
in this bulletin is to the best of our knowledge accurate, but no warranty is expressed or implied. It is the user’s responsibility to determine the suitability for their own use of the products described herein, and since conditions
of use are beyond our control, we disclaim all liability with respect to the use of any material supplied by us. Nothing contained herein shall be construed as a recommendation to use any product or to practice any process in
violation of any law or any government regulation.
© 2014 Polysciences, Inc. Control No. TIV-1-MK-2a Rev. #3 02.20.2014