U.S. Corporate Headquarters
400 Valley Road
Warrington, PA 18976
1(800) 523-2575 / (215) 343-6484
Fax: 1(800)343-3291
Email: [email protected]
www.polysciences.com
Polysciences Europe GmbH
Handelsstrasse 3 D-69214
Eppelheim, Germany
+(49) 6221-765767
Fax: +(49) 6221-764620
Email: [email protected]
Technical Data Sheet 847
Page of 1
20X 10mM Citrate Buffer Kit, pH 6.0
for antigen retrieval FFPE, and resin
DESCRIPTION
Formalin fixation forms protein cross-links that can mask the antigenic sites in tissue specimens, thereby creating weak or
false negative staining for immunohistochemical detection of certain proteins. There are currently several antigen retrieval
methods commonly used and the protocols below only intended as a general guide. Polysciences, Inc. provides AR buffer
kits, that include rinses and pH adjustment reagents for the 20X concentration kit. These kits are recommended for formalin
fixed paraffin embedded tissues.
. Place slides in 500ml of buffer (use a microwavable container)
2. Microwave on high for 15 minutes without a lid or cover with vented cling-film
and heat at 90ºC for 10 minutes (buffer must not boil)
3. Let sections stand for 15 minutes to cool
4. Wash in Tris Buffered Saline (Cat. #24082) and proceed with staining protocol
STORAGE AND HANDLING
Shipment
Room temperature
Storage
Room temperature
Safety
Refer to MSDS for details
All values are considered typical based on tests believed to be accurate. Polysciences, Inc. may change the data as appropriate.
Ordering Information
Cat. #
Description
25079
20X 10mM Citrate Buffer Kit, pH 6.0
(for antigen retrieval FFPE and resin)
To ORDER
In The U.S. Call:
In The U.S. Fax:
1(800) 523-2575 • (215) 343-6484
1(800) 343-3291 • (215) 343-0214
In Germany Call:
In Germany Fax:
+(49) 6221-765767
+(49) 6221-764620
Order online anytime at www.polysciences.com
Should any of our materials fail to perform to our specifications, we will be pleased to provide replacements or return the purchase price. We solicit your inquiries concerning all needs for life sciences work. The information given in this
bulletin is to the best of our knowledge accurate, but no warranty is expressed or implied. It is the user’s responsibility to determine the suitability for their own use of the products described herein, and since conditions of use are beyond
our control, we disclaim all liability with respect to the use of any material supplied by us. Nothing contained herein shall be construed as a recommendation to use any product or to practice any process in violation of any law or any
government regulation.
Technical Data Sheet 847
© Polysciences, Inc. - REV: 06.02.10