2D chloroquine agarose control gels
negatively
supercoiled pRS316
topoIb relaxed pRS316
0.4% agarose 1XTBE
ΔLK0
0.4% agarose 1XTBE
0.5µg/ml chloroquine
linearised
circles
2D analysis of
topoIb relaxed
pRS316 nicked
0.4% agarose 1XTBE
0.5µg/ml chloroquine
1.2% agarose 1XTBE
1µg/ml chloroquine
1.2% agarose 1XTBE
1µg/ml chloroquine
2D analysis of
negatively
supercoiled
nicked
pRS316 circles
circles
ΔLK0
linearised
circles
All gels above stained with EtBr post resolution and photographed
Adapted from figure 2C of Baxter et al
cdc20td top2td pRS316
Release into
+20 mins
Nocodazole
arrested
nicked
circles
ΔLK0
linearised
circles
Cartoon representation
of monomer topoisomers
nicked
circles
ΔLK0
linearised
circles
Blots probed with pRS316 sequences
nicked
circles
ΔLK0
(+)
supercoiled
topoisomers
More (+)
supercoiled
highly (-)
supercoiled
topoisomers