Plastic Labware
PAA – Cell Culture and More
PAA is specialized in the manufacture and worldwide distribution
of cell culture products for research, development, diagnostic and
biopharmaceutical production. For more than 20 years we
accompany our customers in their daily laboratory routine and
provide them with high quality animal and human sera, media,
reagents and buffer solutions. Our products are manufactured in
three facilities based in Austria, Australia and Canada.
In parallel with our investment in facilities and capabilities we
have also committed considerable funds into new product development to satisfy the requests of our research based customers.
We have expanded our product range with new and innovative
media and several new reagents.
Beside the excellent product quality, our main aim is to provide
outstanding customer service. In consequence of this we expanded our product portfolio with disposables such as plastic labware
to enable our customers to purchase their laboratory equipment
conveniently from one source.
The plastic labware is designed by leading manufacturers, using
high precision injection molding, polymer technology and surface
chemistry. Quality characteristics such as the absence of endo­
toxins, non-cytotoxicity as well as exceptional optical clearance
and good cell performance are the basic principles of our cell
culture products.
For the area of cell imaging we provide highly innovative products
for cell based assays, fluorescence microscopy and high content
screening. They feature high plate planarity, low background
interference and high light transmission for challenging experiments.
Our company is known for its focus on high throughput laboratories as well as the support of smaller research groups. Therefore,
we offer our new product line in different purchase units to
satisfy the individual needs of our customers.
Quality Control
The basic requirement for the reproducibility and comparability
for cell and tissue culture in the field of in vitro research is ­first
class quality standard.
Sterility
Sterilization is a process in which surfaces of materials and
objects are freed from living organisms. As a 100 % security can
not be guaranteed, a reduction is required, so that a SAL-value
(Sterility Assurance Level) of 10-6 is reached. This means that in
a million equally treated units of the sterilized products only one
augmentable micro-organism should be included. This SALvalue can be achieved by gamma irradiation, beta irradiation
and ethylene oxide sterilization.
RNA/RNases and DNA/DNases
DNases and RNases belong to a group of enzymes that destroy
nucleic acids. They protect living organisms against the invasion
of foreign DNA and foreign RNA by hydrolyzing the phosphodi­
ester bond between the 5'-phosphate group of a nucleotide and
the 3'-hydroxyl group nearby. DNA/DNases and RNA/RNases have
a great potential for transmission so that protection during working processes is necessary.
Pyrogens
Pyrogens are high molecular weight substances of polymerous
structure which might effect cell growth and proliferation. They
can either be an endotoxin or an exotoxin. Endotoxins are natural
compounds of the cell wall of gram-negative bacteria and potentially toxic. The biological activity of endotoxin is associated with
the lipopolysaccharides (LPS) of the bacterial membrane.
As pyrogens are heat stable and cannot be destroyed by gamma
irradiation it is necessary to test for their absence. The Limulus
Amoebocyte Lysate Assay (LAL assay) is a very sensitive method
for the detection of the presence and quantity of bacterial endotoxins. The LAL test complies with the FDA guidelines.
Cytotoxicity
Cytotoxicity describes the ability of distinct chemical substances
to damage cells or tissues. Especially for reliable toxicological
studies in cell culture research the quality of culture vessels is of
high relevance. It must be guaranteed that they are free of cytotoxic substances to prevent false positive results. The cytotoxicity
is determined by using mammalian cells according to EN ISO
10993.
Materials
Most of our plastic labware is made of either polystyrene or polypropylene. Polystyrene (PS) is a high quality polymer with an
excellent optical clarity. This feature facilitates the optical control
of cells cultivated in PS flasks, wells and dishes. PS is also applicable for the manu­facturing of serological pipettes and tubes where
precise mea-surements are necessary.
Polypropylene (PP) is a linear hydrocarbon polymer used for many
different applications. PP is characterized by a high chemical resis­
tance to many chemical solvents, bases and acids. It shows thermal stability in a temperature range of −80 °C to +121 °C and is
therefore autoclavable. Due to its high mechanical resistance PP
is used for products which are exposed to pressure occurring e.g.
at high speed centrifugation.
Surface Treatment
Our plastic labware used for the cultivation of adherent cells is
plasma treated. This surface technology is based on the use of an
ionized gas at a certain pressure to increase the surface energy.
As a result the polystyrene surface of the cell culture vessels is
more hydrophilic and allows optimal cell attachment.
PAA – The Cell Culture Company | www.paa.com 3
Table of Contents
Cell Culture
Molecular Biology
Cell Culture Flasks . . . . . . . . . . . . . . . . . . . . . . . . . 8
Cell Culture Dishes . . . . . . . . . . . . . . . . . . . . . . . . . 9
Cell Scrapers . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10
Cell Lifter . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10
Multiwell Tissue Culture Plates . . . . . . . . . . . . . . . 11
96 Well Microplates for Colorimetric Assays . . . . . 12
Cell Culture Plates with Inserts . . . . . . . . . . . . . . . 13
C-Chip Neubauer Improved / Malassez . . . . . . . . . . 14
Tray Plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15
Square Dish . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15
Center Well Culture Dish . . . . . . . . . . . . . . . . . . . 16
Confocal Dishes . . . . . . . . . . . . . . . . . . . . . . . . . . 16
Coverslips . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17
Serological Pipettes . . . . . . . . . . . . . . . . . . . . . . . . .
Petri Dishes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Blood Separation Tubes . . . . . . . . . . . . . . . . . . . . .
Conical Centrifuge Tubes . . . . . . . . . . . . . . . . . . . .
2-Position Tubes . . . . . . . . . . . . . . . . . . . . . . . . . . .
Test Tubes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Microtubes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
PCR Tubes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Pipette Tips . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Filter Pipette Tips . . . . . . . . . . . . . . . . . . . . . . . . . Dispenser / Dispenser Tips . . . . . . . . . . . . . . . . . . . Multiwell Plates . . . . . . . . . . . . . . . . . . . . . . . . . . .
Tray Plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Square Dishes . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Erlenmeyer Flasks . . . . . . . . . . . . . . . . . . . . . . . . . .
Loops & Needles . . . . . . . . . . . . . . . . . . . . . . . . . . .
Spreader . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
4 PAA – The Cell Culture Company | www.paa.com
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21
22
23
24
24
25
25
26
27
28
30
31
31
32
33
33
Immunoplates
96 Well Immunoplates (ELISA Plates) . . . . . . . . . . .
Strip Immunoplates . . . . . . . . . . . . . . . . . . . . . . . . .
Black & White Immunoplates . . . . . . . . . . . . . . . . .
384 Well Immunoplates . . . . . . . . . . . . . . . . . . . . .
Handling & Storage
36
36
37
37
Cell Imaging
Imaging Plates CG, with Glass Bottom . . . . . . . . . .
Imaging Plates FC, with Fluorocarbon Film Bottom .
Imaging Dishes CG (Cover Glass) . . . . . . . . . . . . . .
Imaging Dish Stage Frames . . . . . . . . . . . . . . . . . . .
Imaging Chambers / Imaging Chambers CG . . . . . .
Imaging Chamber Accessories . . . . . . . . . . . . . . . .
Cell Culture Slides . . . . . . . . . . . . . . . . . . . . . . . . . .
Slide Tray Plates . . . . . . . . . . . . . . . . . . . . . . . . . . .
CytoCapture . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
41
42
43
43
44
45
46
47
48
Cryotubes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Inserts for Cryotubes . . . . . . . . . . . . . . . . . . . . . . . .
Working Rack for Cryotubes . . . . . . . . . . . . . . . . . .
Cryo Boxes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Storage Boxes . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Storage Boxes with Hinged Cover . . . . . . . . . . . . . .
Conical Centrifuge Tube Racks . . . . . . . . . . . . . . . .
Microtube Racks . . . . . . . . . . . . . . . . . . . . . . . . . . .
PCR Tube Racks . . . . . . . . . . . . . . . . . . . . . . . . . . .
Reservoirs . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Square Bottles . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Autoclave Bags . . . . . . . . . . . . . . . . . . . . . . . . . . . .
54
55
55
56
57
58
59
60
61
62
63
64
Appendix
Alphabetical Index . . . . . . . . . . . . . . . . . . . . . . . . . 68
Material Properties . . . . . . . . . . . . . . . . . . . . . . . . 70
Chemical Resistance of Various Materials . . . . . . . . 71
PAA – The Cell Culture Company | www.paa.com 5
Cell culture is a technique which enables the cultivation of higher organisms. It can serve as
a simplified model to understand the regulation and the biochemistry of eukaryotic cells.
Therefore, PAA offers high quality consumables for performing different kinds of cell culture
procedures. Quality characteristics such as the absence of endotoxins, non-cytotoxicity as
well as excellent optical clearance and good cell performance are the basic principles of our
products.
6 PAA – The Cell Culture Company | www.paa.com
Cell Culture
Cell Culture Flasks . . . . . . . . . . . . . . . . . . . . . . . . . . . 8
Cell Culture Dishes . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
Cell Scrapers . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10
Cell Lifter . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10
Multiwell Tissue Culture Plates . . . . . . . . . . . . . . . . . 11
96 Well Microplates for Colorimetric Assays . . . . . . . 12
Cell Culture Plates with Inserts . . . . . . . . . . . . . . . . . 13
C-Chip Neubauer Improved / Malassez . . . . . . . . . . . . 14
Tray Plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15
Square Dish . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15
Center Well Culture Dish . . . . . . . . . . . . . . . . . . . . . 16
Confocal Dishes . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16
Coverslips . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17
PAA – The Cell Culture Company | www.paa.com 7
Cell Culture
In research cell culture flasks are routinely used for the cultivation of eukaryotic cells. They are first class products for adherent
cells as well as for suspension cultures and applicable for different requirements.
PAA offers standard and filter cap cell culture flasks of excellent
optical clarity. The filter caps are equipped with a high quality
membrane with a pore size of 0.22 µm allowing consistent gas
exchange. Cell culture flasks with green caps are suitable for
adherent cells, providing a surface treated zone for optimal cell
adhesion and growth. Flasks with white caps meet the requirements of suspension cells.
The design of the flasks provides flexibility in media volume and
saves incubator space. In addition, it facilitates the accessibility
with cell scrapers and serological pipettes in an optimal way. The
cell culture flasks are easy to stack and stand firmly in the incubator. The angled neck of the high profile flask reduces the risk
of wetting the inside of the cap with medium and thus provides
additional protection against contamination.
Immunoplates
Molecular Biology
Cell Culture
Cell Culture Flasks
Cell Imaging
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Handling & Storage
Cat. No.
PAA70025X
PAA70075X
PAA71175X
PAA70125X
PAA70175X
PAA72175X
PAA70325X
PAA70375X
PAA74175X
PAA70225X
PAA70275X
PAA73175X
ith standard caps or filter caps available
W
For adherent and suspension cells available
Plasma treated for adherent cells (flasks with green cap)
Sterilized
Angled, wide neck for easy access
Volume graduations on both sides
Large field of inscription for clear identification
Non-pyrogenic
Non-cytotoxic
Material
Cap Type
Cap Colour
Growth
Area (cm2)
Working
Volume (ml)
Surface
Treated
Sterilized
Pieces / Bag
Purchase
Unit (pcs)
PS
PS
PS
PS
PS
PS
PS
PS
PS
PS
PS
PS
Filter
Filter
Filter
Standard
Standard
Standard
Filter
Filter
Filter
Standard
Standard
Standard
Green
Green
Green
Green
Green
Green
White
White
White
White
White
White
25
75
175
25
75
175
–
–
–
–
–
–
7
25
50
7
25
50
7
25
50
7
25
50
+
+
+
+
+
+
–
–
–
–
–
–
+
+
+
+
+
+
+
+
+
+
+
+
5
5
5
5
5
5
5
5
5
5
5
5
200
100
40
200
100
40
200
100
40
200
100
40
8 PAA – The Cell Culture Company | www.paa.com
Cell Culture
PAA20035X
PAA20060X
PAA20101X
PAA20151X
Material
PS
PS
PS
PS
External
Internal
Growth
Working
External Surface
Pieces / Purchase
Sterilized
Dimension (mm) Dimension (mm) Area (cm2) Volume (ml)
Grip
Treated
Bag
Unit (pcs)
35 x 10
60 x 15
100 x 20
150 x 20
34.3 x 9.3
52.7 x 12.6
88.0 x 16.0
138.5 x 15.4
9.4
21.5
60.8
148.0
3.0
5.0
12.5
35.0
+
+
+
−
+
+
+
+
+
+
+
+
10
10
10
10
Molecular Biology
500
500
200
120
Cell Imaging
Cat. No.
Available in four different sizes (Ø 35, 60, 100, 150 mm)
E asy grip for simplified aseptic handling
Optically clear for microscopy
Optimal gas exchange due to ventilation cams in the lid
Plasma treated for optimal growth conditions
Sterilized
For adherent and suspension cells
Safe and stable storage due to raised stacking rings
Non-pyrogenic
Non-cytotoxic
For a successful cell culture ...
... the use of high quality products is inevitable. PAA is a
reliable partner, offering a broad range of animal and
human sera, standard and cell type specific media, buffer
solutions and reagents such as antibiotics, detachment
solutions, growth supplements and others. Our online
catalogue provides you with detailed information at:
www.paa.com/products/cell_culture_products.html
PAA – The Cell Culture Company | www.paa.com 9
Handling & Storage
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Immunoplates
Cell culture dishes are applicable as alternative to cell culture
flasks and appropriate for different requirements.
PAA offers cell culture dishes of excellent optical clarity. High
quality surface treatment of the growth area enables constant
results. Easy stackability ensures a firm stand in the incubator.
A raised ring around the circumference of the dish provides a
secure grip of dish and lid. This reduces the risk of contamination due to unintentional removal of the lid. Cams inside the lid
allow constant ventilation in a closed system.
Cell Culture
Cell Culture Dishes
Cell Culture
Cell scrapers and lifters allow an efficient and gentle removal of
adherent cells from the cultivation surface. They can be used as
an alternative or in addition to enzymatic detachment solutions.
The cell scrapers are designed for optimal contact to the culture
vessel and appropriate to flasks, dishes and plates. Even in culture
containers with poorly accessible surfaces the free rotatability of
the blade enables easy and complete scraping of attached cells.
The thin and flexible blades of the scrapers are made of polyethylene, a material that does not affect the cells.
The cell lifter is especially suitable for gentle harvesting of cells
and tissues from dishes and plates. The one piece design with a
small and a big blade on one handle facilitates working with
diverse culture vessels.
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Cell Scrapers
Cat. No.
PAA90020X
PAA90030X
PAA90040X
Adjustable blade for optimal flexibility (cell scraper)
Two blades for several well formats (cell lifter)
Sterilized
Non-pyrogenic
Single packaged
Cell Lifter
Material
(handle)
Material
(blade)
Blade Width (cm)
Total Length (cm)
Sterilized
Pieces / Bag
Purchase Unit (pcs)
PP
PP
PP
PE
PE
PP
1.3
2.0
1.2 / 2.3
23.7
30.0
21.8
+
+
+
1
1
1
100
100
100
Handling & Storage
Cell Imaging
Immunoplates
Molecular Biology
Cell Culture
Cell Scrapers & Cell Lifter
10 PAA – The Cell Culture Company | www.paa.com
Cell Culture
the inner side of the lid allows safer sample handling. Thus the
risk of contamination from sample material being carried over is
minimized. An alphanumeric well coding ensures easy sample
identification. A plasma treated or non-treated version can be
selected for each multiwell plate (except 4 well plate) depending
on the experiments.
U-bottom
> Round bottom shape
> No sharp corners for easy
pipetting and cleaning
> Suitable for +/− analyses
Cat. No.
PAA30004
PAA30004X
PAA30006X
PAA30012X
PAA30024X
PAA30048X
PAA30096X
PAA34096X
2
F-bottom
> Flat bottom shape
> For precise optical
measurements
> For microscopic applications
Cell Imaging
1
2
Optically clear for microscopy
Lid with condensation rings (except PAA30004, PAA30004X)
Ergonomic grip for simplified aseptic handling
Optimal gas exchange due to ventilation cams in the lid
Stackable and space saving design
For adherent and suspension cells
Sterilized
Non-pyrogenic
96 well with U- or F-bottom
Well
External
Well Dimension Growth
Working
Surface
Pieces /
Material
Sterilized
Format
Dimension (mm)
Ø (mm)
Area (cm²) Volume (ml) Treated
Bag
4F
4F
6F
12 F
24 F
48 F
96 F
96 U
PS
PS
PS
PS
PS
PS
PS
PS
66.0 x 66.0
66.0 x 66.0
85.4 x 127.6
85.4 x 127.6
85.4 x 127.6
85.4 x 127.6
85.4 x 127.6
85.4 x 127.6
15.8
15.8
35.0
22.1
15.6
9.75
6.4
6.4
2.0
2.0
9.6
3.8
1.9
0.75
0.32
0.32
1.0
1.0
3.0
2.0
1.0
0.5
0.2
0.2
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
4
4
1
1
1
1
1
1
Purchase
Unit (pcs)
4
120
50
50
50
50
50
50
PAA32006X
6F
PS
85.4 x 127.6
35.0
9.6
3.0
–
+
1
50
PAA32012X
PAA32024X
PAA32048X
PAA32096X
12 F
24 F
48 F
96 F
PS
PS
PS
PS
85.4 x 127.6
85.4 x 127.6
85.4 x 127.6
85.4 x 127.6
22.1
15.6
9.75
6.4
3.8
1.9
0.75
0.32
2.0
1.0
0.5
0.2
–
–
–
–
+
+
+
+
1
1
1
1
50
50
50
50
PAA34296X
96 U
PS
85.4 x 127.6
6.4
0.32
0.2
–
+
1
50
PAA – The Cell Culture Company | www.paa.com 11
Handling & Storage
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Immunoplates
Molecular Biology
Multiwell tissue culture plates are used in all areas of cell culture
and molecular biology.
PAA provides multiwell plates in several sizes and for different
applications. All of them are cell culture tested and designed for
reduced cross contamination risk. The arrangement of each well
standing on its own and corresponding condensation rings on
Cell Culture
Multiwell Tissue Culture Plates
Cell Culture
Colorimetric assays, e.g. fluorescence or luminescence measurements are more and more common in cell culture research. To
avoid negative side effects such as background interference,
autofluorescence or cross talk the use of black or white pigmented microplates is recommended.
PAA provides two different high quality 96 well microplates,
suitable also for various high throughput assays. High signal-tonoise ratio with low background is a characteristic feature of
these plates.
The 96 well microplates are cell culture tested and designed for
reduced cross contamination risk. The arrangement of each well
standing on its own and corresponding condensation rings on
the inner side of the lid allow safe sample handling. Thus the risk
of contamination from sample material being carried over is
minimized. An alphanumeric well coding ensures easy sample
identification.
Immunoplates
Molecular Biology
Cell Culture
96 Well Microplates for Colorimetric Assays
Cell Imaging
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Cat. No.
Material
Plate /
Lid Color
External
Dimension
(mm)
PS
PS
PS
PS
White / Trans.
White / Trans.
Black / Black
Black / Black
85.4 x 127.6
85.4 x 127.6
85.4 x 127.6
85.4 x 127.6
Handling & Storage
PAA30196
PAA30196X
PAA30296
PAA30296X
Available in white and black
High signal-to-noise ratio
Low background interference
Plasma treated for optimal growth conditions
Non-transparent flat bottom
Sterilized
Single packaged
12 PAA – The Cell Culture Company | www.paa.com
Well
Growth Total Vol. / Surface
Pieces / Purchase
Dimension
Sterilized
Area (cm²) Well (µl) Treated
Bag Unit (pcs)
Ø (mm)
6.3
6.3
6.3
6.3
0.32
0.32
0.32
0.32
400
400
400
400
+
+
+
+
+
+
+
+
1
1
1
1
1
50
1
50
Cell Culture
cise pore diameter and a consistent pore size in experiments.
The inserts are available with pores of 0.4 µm, 3.0 µm and
8.0 µm diameter. Due to their transparency and thinness the
inserts with PET membrane are suitable for microscopic works.
The PC membrane features excellent staining, inertness and
ensures low background interference.
Immunoplates
Molecular Biology
Cell culture inserts are used in a variety of applications including
invasion, migration, transport, drug uptake, cellular polarization, chemotaxis, co-culture experiments and toxicity studies.
PAA offers cell culture inserts with PC or PET membrane that
closely mimic an in vivo environment, resulting in improved cell
attachment, growth and differentiation of various cell types. The
special manufacturing process of the membrane ensures a pre-
Cell Culture
Cell Culture Plates with Inserts
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Cat. No.
PAA35006X
PAA35106X
PAA35206X
PAA35024X
PAA35124X
PAA35224X
PAA37006X
PAA37106X
PAA37206X
PAA37024X
PAA37124X
PAA37224X
Membrane
Membrane
Material Pore Size (µm)
PC
PC
PC
PC
PC
PC
PET
PET
PET
PET
PET
PET
0.4
3.0
8.0
0.4
3.0
8.0
0.4
3.0
8.0
0.4
3.0
8.0
Membrane
Diameter (mm)
Growth
Area (cm2)
24.0
24.0
24.0
6.5
6.5
6.5
24.0
24.0
24.0
6.5
6.5
6.5
4.52
4.52
4.52
0.33
0.33
0.33
4.52
4.52
4.52
0.33
0.33
0.33
Cell Imaging
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Convenient usage
Consistent pore size
Compatible with automated systems
Packaged with multiwell plate
(one plate per bag)
Distance from membrane to the bottom
of the well: 0.8 – 1 mm
Nominal membrane thickness: 7 – 10 µm
Pore Size: 0.4, 3.0 or 8.0 μm
Membrane material: PC or PET
Sterilized
Working
Sterilized
Volume (ml)
Well
Format
Inserts /
Plate
Purchase
Unit (plates)
1.5 – 2.5
1.5 – 2.5
1.5 – 2.5
0.2 – 0.35
0.2 – 0.35
0.2 – 0.35
1.5 – 2.5
1.5 – 2.5
1.5 – 2.5
0.2 – 0.35
0.2 – 0.35
0.2 – 0.35
6 well
6 well
6 well
24 well
24 well
24 well
6 well
6 well
6 well
24 well
24 well
24 well
6
6
6
12
12
12
6
6
6
12
12
12
4
4
4
4
4
4
4
4
4
4
4
4
+
+
+
+
+
+
+
+
+
+
+
+
PAA – The Cell Culture Company | www.paa.com 13
Handling & Storage
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Cell Culture
Hemocytometers are used in daily routine in cell culture, microbiology and haematology. The preparation of traditional glass
chambers is a time-consuming and not reliably reproducible
process that often ends with broken cover slips.
PAA offers with the C-Chip Neubauer Improved and Malassez
two disposable plastic hemocytometers for manual cell counting.
They are very easy to use and the precise volume control leads
to highly reproducible results. Two counting chambers are fitted
with a clear grid pattern rich in contrast. The productivity is
increased by eliminating cleaning steps. Redundancy of the
cleaning step also reduces the risk of exposure to potentially
infectious material.
Immunoplates
Molecular Biology
Cell Culture
C-Chip Neubauer Improved / Malassez
Handling & Storage
Cell Imaging
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Neubauer Improved
Easy to use
Precise quantification
High reproducibility
No need for cover slips
No need for cleaning and reuse
Time and cost saving
Quartz grade optical plastic
Clear grid pattern
Very light and nearly unbreakable
Single packaged
Malassez
Grid
Chambers
per Slide
PAA404040X
Malassez
2
200 µm
20 µl
25 x 75 x 1.6
1
50
PAA505050X
Neubauer Improved
2
100 µm
10 µl
25 x 75 x 1.6
1
50
Cat. No.
14 PAA – The Cell Culture Company | www.paa.com
Chamber Depth Chamber Volume Dimensions (mm) Pieces / Bag
Purchase Unit
(pcs)
Cell Culture
Cat. No.
Material
PAA30001X
PS
External
Dimension (mm)
Optically clear
High plate planarity
Surface treated for tissue culture
Sterilized
Single packaged
Internal
Dimension (mm)
127.6 x 85.6 x 16.2 109.5 x 73.5 x 11.6
Growth
Area (cm2)
Surface
Treated
Sterilized
Pieces /
Bag
Purchase
Unit (pcs)
80
+
+
1
50
Square Dish
Square dishes are commonly used in cell culture applications,
especially for mammalian cell culture. PAA offers a tissue culture
treated square dish made of optically clear polystyrene. It is
designed and tested for standard HTS systems for automated
colony picking. The large surface area and the gridded bottom
are useful for tracing of individual cell colonies. The grid consists
of thirty-six squares with alphanumerical coding.
Large surface area
Optically clear
High plate planarity
Available with grids for determination
of plating efficiency
> Surface treated for tissue culture
> Alphanumerical coding
> Sterilized
Cat. No.
PAA10125X
Cell Imaging
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Molecular Biology
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Immunoplates
surface area compared to round type dishes and easy handling
for any application. The external dimension of this single well
plate is identical to standard multiwell plates.
Material
External
Dimension (mm)
Internal
Dimension (mm)
Growth
Area (cm2)
Surface
Treated
Sterilized
Pieces /
Bag
Purchase
Unit (pcs)
PS
125 x 125 x 20
118.7 x 118.7 x 16.5
139
+
+
5
60
PAA – The Cell Culture Company | www.paa.com 15
Handling & Storage
Tray plates are highly suitable for multiple cell culture applications.
PAA provides a tray plate that can be used for mammalian cell
culture. The rectangular shape of the tray dish provides a larger
Cell Culture
Tray Plate
Cell Culture
For the research of in vitro fertilization on animal cells PAA offers
a convenient center well culture dish.
The 60 mm x 15 mm dish features a 20 mm center well for
oocyte culture and insemination, surrounded by a larger moat
for humidification. The center well holds 3 ml of medium while
Cat. No.
For research only
Optical grade polystyrene
Easy grip for secure handling
Surface treated for tissue culture
Sterilized
Non-pyrogenic
Non-cytotoxic
Material
External
Dimension (mm)
Internal
Dimension (mm)
Surface
Treated
Sterilized
Pieces /
Bag
Purchase
Unit (pcs)
PS
60 x 15
20
+
+
10
500
PAA20260X
Confocal Dishes
The in vitro cultivation of cells under laboratory conditions is
common practice for a huge variety of applications. The range
of cell types is almost limitless, e.g. plant and insect cells as well
as avian and mammalian cells.
PAA offers confocal dishes made of polystyrene with coverglass
bottom or removable coverglass insert. The dishes facilitate
preparation of cells for microscopic analyses. They are suitable
for use in high resolution and inverted microscopy, fluorescence
imaging in living cells, confocal microscopy, phase contrast
microscopy and micro-manipulation or anywhere where highest
image quality is desired. A raised ring around the circumference
of the dish provides a secure grip of dish and lid, reducing the
risk of contamination due to unintentional removal of the lid.
Cell Imaging
Immunoplates
the outer well holds 10 ml. The center well culture dishes are
made of optical grade polystyrene for clear visualization by
microscope. The easy grip provides convenient and secure handling. The dishes are tissue culture treated and sterile.
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Molecular Biology
Cell Culture
Center Well Culture Dish
> Facilitated preparation of cells for
microscopic analyses
> Optically clear with low background
fluorescence
> Easy grip for secure handling
> Sterilized
> Hole size Ø: 13 mm
> Coverglass size: 22 x 22 mm
> Coverglass thickness: 0.13 ~ 0.16 mm
Handling & Storage
Bottom Type
Cat. No.
PAA100350
PAA100350X
PAA200350
PAA200350X
PAA100351
PAA100351X
Insert Type
Material
External
Dimension (mm)
Type
PS
PS
PS
PS
PS
PS
35 x 10
35 x 10
35 x 10
35 x 10
35 x 10
35 x 10
Coverglass bottom
Coverglass bottom
Coverglass bottom
Coverglass bottom
Coverglass insert
Coverglass insert
16 PAA – The Cell Culture Company | www.paa.com
Growth
Working
Surface
Pieces / Purchase
Sterilized
Area (cm2) Volume (ml) Treated
Bag
Unit (pcs)
9.4
9.4
9.4
9.4
9.4
9.4
3.0
3.0
3.0
3.0
3.0
3.0
–
–
+
+
–
–
+
+
+
+
+
+
5
5
5
5
10
10
5
100
5
50
10
500
Cell Culture
>
>
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crystal clear and features excellent chemical resistance. In contrast to glass, Aclar™ coverslips are UV light transmissive and
resistant to breakage. The material provides easy cell attachment
and growth.
Great microscopic properties
Resistant to breakage
Excellent chemical resistance
Optimal cell attachment and growth
Facilitates staining and fixation of cells
110 µm thickness
Non autoclavable
Sterilized
Cat. No.
Material
Dimension (mm)
Sterilized
Pieces / Bag
Purchase Unit (pcs)
PAA20009X
PAA20012X
PAA20018X
PAA20025X
Aclar™
Aclar™
Aclar™
Aclar™
9 x 0.11
12 x 0.11
18 x 0.11
25 x 0.11
+
+
+
+
100
100
100
100
200
200
200
200
Molecular Biology
PAA offers disposable coverslips made of Aclar™, suitable for
staining cultured cells and high magnification microscopy.
Aclar™ is a polychlorotrifluoroethylene (PCTFE) material useful
for both tissue culture and electron microscopic purposes. It is
Cell Culture
Coverslips
Cell Imaging
Immunoplates
Aclar™ is a trademark of Honeywell International Inc.
FACS analysis (Fluorescence Activated Cell Sorting) is a specialized form of flow cytometry, where cells can be sorted one cell at a time. A major problem in eukaryotic cell
culture is the clump formation of cells. AccuMax™ is a ready to use enzymatic dissociation solution containing proteolytic, collagenolytic and DNase enzymes. The raw
material is derived from crustaceans and hence a potential contamination risk is reduced to a minimum. Read more about AccuMax™ at
www.paa.com/cell_culture_products/reagents/detachment/accumax.html
AccuMax™ is a trademark of ICT
PAA – The Cell Culture Company | www.paa.com 17
Handling & Storage
AccuMax™ – the basis for accurate cell counting
Molecular biology is the study of biological activity on a molecular level. It includes the interaction between DNA, RNA and proteins and its regulation. PAA provides plastic consumables
such as microtubes, pipette tips and serological pipettes that are essential for many different
applications.
18 PAA – The Cell Culture Company | www.paa.com
Molecular Biology
Serological Pipettes . . . . . . . . . . . . . . . . . . . . . . . . . . .
Petri Dishes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Blood Separation Tubes . . . . . . . . . . . . . . . . . . . . . . .
Conical Centrifuge Tubes . . . . . . . . . . . . . . . . . . . . . .
2-Position Tubes . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Test Tubes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Microtubes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
PCR Tubes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Pipette Tips . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Filter Pipette Tips . . . . . . . . . . . . . . . . . . . . . . . . . . . Dispenser / Dispenser Tips . . . . . . . . . . . . . . . . . . . . . Multiwell Plates . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Tray Plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Square Dishes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Erlenmeyer Flasks . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Loops & Needles . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Spreader . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
20
21
22
23
24
24
25
25
26
27
28
30
31
31
32
33
33
PAA – The Cell Culture Company | www.paa.com 19
Molecular Biology
Serological pipettes are an essential tool for precise volume
measurement. In contrast to glass, plastic pipettes provide the
advantage of constant sterility due to individual packaging.
PAA offers disposable serological pipettes that are single
wrapped in plastic/paper packaging with peel-off function for
aseptic pipette removal. The serological pipettes are also availa-
ble in bulk packages, both sterile and non-sterile. High grade
polystyrene provides maximum transparency. To avoid suction of
liquid into the pipetting device all pipettes are supplied with a
cotton plug. A color code, following international standards,
facilitates the identifi­cation of the pipette size. The slim pipette
tip and the small diameter allow outstanding accessibility.
Handling & Storage
Cell Imaging
Immunoplates
Molecular Biology
Cell Culture
Serological Pipettes
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Cat. No.
PAA92001X
PAA92002X
PAA92005X
PAA92010X
PAA92025X
PAA92050X
PAA93005X
PAA93010X
PAA93025X
PAA94005X
PAA94010X
PAA94025X
High optical clarity
Maximum accuracy
Drop free pipetting
Color coded label (international code)
Increased volume from negative graduations
Black colored graduation
Sterilized
Non-pyrogenic
Single packaged
Volume (ml)
Color Code
Material
Sterilized
Pieces / Bag
Purchase Unit (pcs)
1
2
5
10
25
50
5
10
25
5
10
25
Yellow
Green
Blue
Orange
Red
Violet
Blue
Orange
Red
Blue
Orange
Red
PS
PS
PS
PS
PS
PS
PS
PS
PS
PS
PS
PS
+
+
+
+
+
+
+
+
+
–
–
–
1
1
1
1
1
1
25
25
10
25
25
10
500
500
200
200
150
100
500
400
150
500
400
150
20 PAA – The Cell Culture Company | www.paa.com
Molecular Biology
of dish and lid, reducing the risk of contamination due to unintentional removal of the lid. Cams inside the lid allow constant
ventilation in a closed system.
For special applications PAA offers a Bl-plate with a divided area
for handling two types of microbes and a gridded bottom dish
that facilitates the counting of colonies. Furthermore, a contact
plate is available that is designed to allow a raised convex surface, when filled with agar, for the detection and counting of
microbes from environmental surfaces.
Immunoplates
Molecular Biology
PAA provides a large range (35 mm to 150 mm) of petri dishes
for various laboratory purposes. They are primarily useful to
grow bacteria, fungi and other microorganisms, but also applicable for seed germination and small animal observations. In
addition, they are suitable for other day-to-day laboratory practices such as storing samples.
All of the petri dishes are produced with optically clear high
quality polymers. Easy stackability ensures a firm stand. A raised
ring around the circumference of the dish provides a secure grip
Cell Culture
Petri Dishes
Material
External
Dimension (mm)
PAA10035X
PS
35 x 10
34.3 x 9.3
PAA10060X
PS
52.7 x 12.6
PAA10061X
PS
–
PAA10090X
PAA10091X
PAA10092X
PAA10095X
PAA10100X
PAA10101X
PAA10150X
PAA10151X
PS
PS
PS
PS
PS
PS
PS
PS
60 x 15
60 x 15
(Contact plate)
90 x 15
90 x 20
90 x 15 (BI-plate)
90 x 15 (Grid)
100 x 15
100 x 20
150 x 20
150 x 25
85.2 x 12.6
86.2 x 17.6
–
85.2 x 12.6
96.2 x 13.4
88.0 x 16.0
138.5 x 15.4
138.5 x 23.2
Cat. No.
Internal
Dimension (mm)
Growth
Area (cm2)
Cell Imaging
Optically clear for microscopy
Optimal gas exchange due to ventilation cams in the lid
Easy grip for simplified aseptic handling (PAA10035X, PAA10060X, PAA10101X)
Divided area for handling two types of microbes (PAA10092X)
Easy detection and counting with the contact plate (PAA10061X)
Safe and stable storage due to raised stacking rings
Sterilized
User-friendly packaging
External
Grip
Sterilized
Pieces /
Bag
Purchase
Unit (pcs)
9.4
+
+
20
500
21.5
+
+
20
500
–
–
+
20
500
57.5
58.0
58.0
57.5
72.3
60.8
148.0
148.0
−
–
–
–
–
+
–
–
+
+
+
+
+
+
+
+
10
10
20
10
10
10
10
5
500
200
500
200
500
200
120
120
Dishes for cell culture purposes can be found on page 9.
PAA – The Cell Culture Company | www.paa.com 21
Handling & Storage
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Molecular Biology
The isolation of cells or subcellular particles is the first step for
diagnostics or investigations in cell functionality or gene expression. The enrichment of cells from complex biological mixtures
such as blood and other body fluids requires the use of special
separation media. The blood sample has to be poured carefully
over the separation medium in a time consumptive and
­laborious procedure.
To simplify this process PAA provides a centrifuge tube with an
incorporated porous barrier. This feature prevents a mixture of
the sample material with the separation medium and a recontamination of the enriched cell fraction with unwanted blood
components after centrifugation.
Handling & Storage
Cell Imaging
Immunoplates
Molecular Biology
Cell Culture
Blood Separation Tubes
>
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Cat. No.
PAA535710
PAA535710X
Simplified filling
Rapid separation
Precise and easy harvesting
No recontamination with unwanted blood components
Centrifugation up to 840 x g
Sterilized
Material of barrier: PE
Volume (ml) Material Tube / Cap
50
50
PP / HDPE
PP / HDPE
External Dimension
(mm)
Sterilized
30 x 115
30 x 115
+
+
Separation of lymphocytes – easily done with LSM 1077
For the separation of lymphocytes or other cells out of blood special separation
media are required. The media are used for the production of density gradients for
the separation of blood components, which sediment during centrifugation due to
gravity. PAA offers the ready to use separation solution LSM 1077 based on ficoll
with a density of 1.077 g/ml. Optimal osmotic conditions and a physiological pH level
assure a separation without any loss of viability of the cells. For more information visit
www.paa.com/cell_culture_products/reagents/lymphocyteseparation.html
22 PAA – The Cell Culture Company | www.paa.com
Pieces / Bag Purchase Unit (pcs)
25
25
25
500
Molecular Biology
tubes withstand centrifugal forces up to 3000 x g respectively
9000 x g. For high speed centrifugation a specially designed
40 ml tube is available. For convenient handling PAA also offers
a skirted, self-standing 50 ml tube.
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Available in 15, 40 and 50 ml size
Excellent optical clarity and chemical resistance
Sterile packaged in bag or rack
Large field of inscription
Capable for freezing
For high speed centrifugation purposes
(PAA50040 and PAA50040X)
> Skirted, self-standing type (PAA50250 and PAA50250X)
> Non-pyrogenic
Immunoplates
Molecular Biology
Conical centrifuge tubes are used for different applications in
laboratories. PAA provides high strength and non toxic conical
tubes in three different sizes to meet the various demands of
scientists. They are made of polypropylene or polystyrene and
sterile packaged in bags or already sorted in racks. The conical
Cell Culture
Conical Centrifuge Tubes
Volume (ml)
Material
Tube / Cap
Max. Centrifuge
Capacity (x g)
External
Dimension (mm)
Sterilized
Pieces /
Bag
Purchase
Unit (pcs)
15
15
40
40
50
50
15
15
50
50
PP / HDPE
PP / HDPE
PP / HDPE
PP / HDPE
PP / HDPE
PP / HDPE
PS / HDPE
PS / HDPE
PP / HDPE
PP / HDPE
5.500
5.500
20.000
20.000
9.000
9.000
3.000
3.000
3.000
3.000
17 x 120
17 x 120
30 x 115
30 x 115
30 x 115
30 x 115
17 x 120
17 x 120
30 x 116
30 x 116
+
+
+
+
+
+
+
+
+
+
50
50
10
10
25
25
50
50
25
25
50
500
10
100
25
500
50
500
25
500
Volume (ml)
Material
Tube / Cap
Max. Centrifuge
Capacity (x g)
External
Dimension (mm)
Sterilized
Pieces /
Rack
Purchase
Unit (pcs)
15
15
50
50
15
15
PP / HDPE
PP / HDPE
PP / HDPE
PP / HDPE
PS / HDPE
PS / HDPE
5.500
5.500
9.000
9.000
3.000
3.000
17 x 120
17 x 120
30 x 115
30 x 115
17 x 120
17 x 120
+
+
+
+
+
+
25
25
25
25
25
25
25
500
25
300
25
500
PAA50015
PAA50015X
PAA50040
PAA50040X
PAA50050
PAA50050X
PAA51015
PAA51015X
PAA50250
PAA50250X
Tubes provided in plastic rack
Cat. No.
PAA50115
PAA50115X
PAA50150
PAA50150X
PAA51115
PAA51115X
PAA – The Cell Culture Company | www.paa.com 23
Handling & Storage
Cat. No.
Cell Imaging
Packaged loosely in bag
Molecular Biology
Cell Culture
2-Position Tubes
PAA offers 2-position tubes in two sizes to meet a wide range
of different demands. Depending on the position of the snap
cap an airtight closure or ventilation of the tube is achieved. The
polypropylene tubes provide high chemical and thermal stability
and are suitable for centrifugation at higher speed. If optical
clearance is required we recommend the use of our polystyrene
tubes.
Available in two sizes
High chemical and thermal stability
Two position snap cap
Suitable for centrifugation
In polypropylene (PP) and polystyrene (PS) available
Sterilized
Molecular Biology
>
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Immunoplates
Cat. No.
PAA40005
PAA40005X
PAA40014
PAA40014X
PAA41005
PAA41005X
PAA41014
PAA41014X
Volume (ml)
Material Tube / Cap
External Dimension (mm)
5
5
14
14
5
5
14
14
PS / HDPE
PS / HDPE
PS / HDPE
PS / HDPE
PP / HDPE
PP / HDPE
PP / HDPE
PP / HDPE
12 x 75
12 x 75
17 x 100
17 x 100
12 x 75
12 x 75
17 x 100
17 x 100
Sterilized Pieces / Bag
+
+
+
+
+
+
+
+
25
25
25
25
25
25
25
25
Purchase Unit (pcs)
25
500
25
500
25
500
25
500
In medicinal and biological research flow cytometry has broad
applications especially in transplantation, hematology, oncology
and genetics. It allows counting, examining and sorting of
microscopic particles suspended in a stream of fluid. For sample
preparation PAA offers polystyrene test tubes of high optical
clarity.
Handling & Storage
Cell Imaging
Test Tubes
Cat. No.
PAA010220
Volume (ml) Material External Dimension (mm) Internal Dimension (mm) Sterilized Pieces / Bag Purchase Unit (pcs)
5
PS
24 PAA – The Cell Culture Company | www.paa.com
12 x 75
10 (+/− 0.1)
−
1000
1000
Molecular Biology
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Molecular Biology
Microtubes are used in every field of research and diagnostics.
PAA provides microtubes made of high quality and high strength
polypropylene to meet the demand of each application. Smooth
opening and tight sealing as well as high chemical resistance are
their main features. The high mechanical resistance permits high
speed centrifugation.
Cell Culture
Microtubes, 1.5 ml and 2.0 ml
Temperature range -80 °C to +121 °C
Smooth opening and tight sealing
High chemical resistance
Autoclavable
Centrifugation up to 11.000 x g
Amber color for storage of light sensitive material
(PAA62015 / PAA62015X)
Color
Material
Sterilized
Pieces / Bag
Purchase Unit (pcs)
1.5
1.5
1.5
1.5
1.5
1.5
1.5
1.5
1.5
2.0
Transparent
Transparent
Transparent
Yellow
Green
Red
Blue
Amber
Amber
Transparent
PP
PP
PP
PP
PP
PP
PP
PP
PP
PP
+
+
−
−
−
−
−
–
–
–
250
250
1000
1000
1000
1000
1000
500
500
1000
250
5000
1000
1000
1000
1000
1000
500
5000
1000
PCR Tubes, 0.2 ml and 0.5 ml
The polymerase chain reaction is a well-established technique to
amplify DNA molecules. For this application PAA provides PCR
tubes that are suitable for most major brands of thermocyclers.
Due to their even and ultrathin well surface the PCR tubes are
excellent for homogeneous and efficient heat transfer.
>H
igh quality reaction tubes with uniformly thin wall
for quick and equal temperature exchange
> Applicable for standard thermocycler types
> Free of DNA
> Free of RNases and DNases
> Autoclavable
Cat. No.
PAA670305X
PAA01051
Volume (ml)
Color
Material
Sterilized
Pieces / Bag
Purchase Unit (pcs)
0.2
0.5
Transparent
Transparent
PP
PP
−
−
1000
1000
1000
1000
PAA – The Cell Culture Company | www.paa.com 25
Cell Imaging
PAA60115
PAA60115X
PAA01021T
PAA01021Y
PAA01021G
PAA01021R
PAA01021B
PAA62015
PAA62015X
PAA01031
Volume (ml)
Handling & Storage
Cat. No.
Immunoplates
2.0 ml Microtube
Molecular Biology
Cell Culture
Pipette Tips
Pipette tips are essential tools in molecular biology and chemistry research and diagnostic. PAA offers pipette tips made of
high quality polypropylene in different sizes for various purposes. A special ionization process allows dust free tip production.
The precise tips provide high quality in any detail, e.g. excellent
volumetric accuracy and optimum handling due to the straight
Molecular Biology
>
>
>
>
shape. This results in easy use when fitting or removing from the
pipette. They are compatible with common pipette brands. The
pipette tips cover the range of 0.1 µl to 10 ml and are available
in bags, sterile multiracks or space-saving multitrays, where
contamination free transfer to a rack is possible.
Minimum fluid retention
Excellent transparency
Compatible with most common pipettes
Autoclavable
Multitray
Cell Imaging
Immunoplates
Multibox
(PAA010200)
with MultiTray
Multirack
Cat. No.
Sample
Volume (µl)
Color
Packaging
Sterilized
Pack Size
(pcs / pack)
Purchase
Unit (pcs)
PAA010101
PAA010102X
PAA010103X
PAA010104
PAA010111
PAA010112X
PAA010113X
PAA010114X
PAA010121
PAA010122X
PAA010123X
PAA010124
PAA010131
PAA010132X
PAA010133X
PAA010134X
0.1 – 10
0.1 – 10
0.1 – 10
0.1 – 10
0.1 – 10
0.1 – 10
0.1 – 10
0.1 – 10
1 – 200
1 – 200
1 – 200
1 – 200
200 – 1000
200 – 1000
200 – 1000
200 – 1000
Transparent
Transparent
Transparent
Transparent
Transparent
Transparent
Transparent
Transparent
Yellow
Yellow
Yellow
Yellow
Blue
Blue
Blue
Blue
Bag
Multirack
Multirack
MultiTray
Bag
Multirack
Multirack
MultiTray
Bag
Multirack
Multirack
MultiTray
Bag
Multirack
Multirack
MultiTray
–
–
+
–
–
–
+
–
–
–
+
–
–
–
+
–
1000
96
96
10 x 96
1000
96
96
2 x 96
1000
96
96
10 x 96
1000
60
60
2 x 60
1000
10 x 96
10 x 96
10 x 96
1000
10 x 96
10 x 96
10 x 96
1000
10 x 96
10 x 96
10 x 96
1000
10 x 60
10 x 60
16 x 60
PAA010141
2 ml – 10 ml
Transparent
Bag
–
100
100
Material
Packaging
Sterile
Purchase Unit (pcs)
PP
Box only
–
1
Handling & Storage
Multibox for all MultiTray Pipette Tips
Cat. No.
PAA010200
26 PAA – The Cell Culture Company | www.paa.com
Molecular Biology
> Free of RNase and DNase
> Minimized fluid retention
> Compatible with many major pipette brands
Color
Packaging
Sterilized
Pack Size
(pcs / pack)
Purchase
Unit (pcs)
PAA010153X
0.1 – 10
Transparent
Rack
+
96
10 x 96
PAA010163X
1 – 20
Transparent
Rack
+
96
10 x 96
PAA010173X
1 – 100
Transparent
Rack
+
96
10 x 96
PAA010183X
1 – 200
Transparent
Rack
+
96
10 x 96
PAA010193X
100 – 1000
Transparent
Rack
+
96
10 x 96
Cell Imaging
Sample
Volume (µl)
Handling & Storage
Cat. No.
Molecular Biology
They are compatible with many common pipette brands. The
sterile filter tips cover a range from 0.1 µl to 1 ml and are available in racks.
Immunoplates
PAA offers also high-quality pipette tips with hydrophobic HDPE
filters providing an aerosol barrier and protection against contamination of pipettes and samples in the aspiration process.
Cell Culture
Filter Pipette Tips
PAA – The Cell Culture Company | www.paa.com 27
Molecular Biology
Dispenser and dispenser tips are implemented in the daily laboratory routine where serial working steps are required. PAA
offers a dispenser for working with dispenser tips. The total
Molecular Biology
Cell Culture
Dispenser
volume, depending on the size of the tips, can be stored and
after adjustment of the dispenser the precise volume can be
released in single steps with a minimum aliquot volume of 1 µl.
Five easy-to-select settings
control the aliquot volume
Dispensing lever designed
for smooth dispensing
Designed for both left and
right hand operation
Filling knob for easy
filling of the tip
Lock knob for safe
mounting of the tip
Easy insertion of
dispenser tip
Cat. No.
Purchase Unit (pcs)
Handling & Storage
Cell Imaging
Immunoplates
PAA020100
1
Dispenser Tips & Dispenser Tips Professional
PAA’s dispenser tips and dispenser tips professional are made of
high quality polypropylene and cover a range of 0.05 ml to 50 ml
for various purposes.
>
>
>
>
While working with 25 ml and 50 ml tips, the use of the adapter is necessary. The dispenser tips as well as the adapter are
povided sterile.
Maximum accuracy
Free of DNase and RNase
Non-pyrogenic
Individually packed
Dispenser tips are compatible with PAA’s dispenser and:
ripette™
HandyStep™
HandyStep™ electronic
Multipette™ 4780
Distriman™
Dispenser tips professional are compatible with:
ripette™
ripette™ genX
HandyStep™
HandyStep™ electronic
Multipette™ 4780
Multipette™ plus (display will be inactive)
ripette™ is a trademark of Ritter GmbH
HandyStep™ is a trademark of Brand GmbH + Co. KG
Multipette™ is a trademark of Eppendorf AG
Distriman™ is a trademark of Gilson Inc.
28 PAA – The Cell Culture Company | www.paa.com
Molecular Biology
Min. Volume (µl)
Max. Volume (ml)
Sterilized
Pieces / Bag
Purchase Unit (pcs)
1
0.05
+
1
100
100
PAA020111X
10
0.5
+
1
PAA020112X
25
1.25
+
1
100
PAA020113X
50
2.5
+
1
100
PAA020114X
100
5.0
+
1
100
PAA020115X
250
12.5
+
1
100
500
25.0
+
1
25
1000
50.0
+
1
25
+
3
3
Pieces / Bag
Purchase Unit (pcs)
100
PAA020116X
Adapter necessary
PAA020117X
Adapter necessary
PAA020120X
Adapter
Dispenser Tips Professional
Cat. No.
Min. Volume (µl)
Max. Volume (ml)
Sterilized
PAA020210X
1
0.1
+
1
PAA020211X
2
0.2
+
1
100
PAA020212X
5
0.5
+
1
100
10
1.0
+
1
100
25
2.5
+
1
100
PAA020215X
50
5.0
+
1
100
PAA020216X
100
10.0
+
1
100
500
25.0
+
1
25
1000
50.0
+
1
25
Handling & Storage
PAA020217X
Adapter included
PAA020218X
Adapter included
Cell Imaging
PAA020213X
PAA020214X
Molecular Biology
PAA020110X
Immunoplates
Cat. No.
Cell Culture
Dispenser Tips
PAA – The Cell Culture Company | www.paa.com 29
Molecular Biology
Multiwell plates are used in various areas of molecular biology.
PAA provides multiwell plates in several sizes and for different
applications. The arrangement of each well standing on its own
and corresponding condensation rings on the inner side of the
lid allows safer sample handling. Thus the risk of contamination
from sample material being carried over is minimized.
An alphanumeric well coding ensures easy sample identification.
Immunoplates
Molecular Biology
Cell Culture
Multiwell Plates
Cell Imaging
>
>
>
>
>
1
1
2
U-bottom
> Round bottom shape
> No sharp corners for easy
pipetting and cleaning
> Suitable for +/− analyses
2
F-bottom
> Flat bottom shape
> For precise optical
measurements
> For microscopic applications
Well
Format
Material
External
Dimension (mm)
Well Dimension
Ø (mm)
PAA31006X
6F
PS
85.4 x 127.6
35.0
9.6
3.0
1
50
PAA31012X
PAA31024X
PAA31048X
PAA31096X
12 F
24 F
48 F
96 F
PS
PS
PS
PS
85.4 x 127.6
85.4 x 127.6
85.4 x 127.6
85.4 x 127.6
22.1
15.6
9.75
6.4
3.8
1.9
0.75
0.32
2.0
1.0
0.5
0.2
1
1
1
10
50
50
50
100
PAA34196X
96 U
PS
85.4 x 127.6
6.4
0.32
0.2
10
100
Cat. No.
Handling & Storage
Optically clear for microscopy
Lid with condensation rings
Safe grip for simplified handling
Stackable and space saving design
96 well with U- or F-bottom
Multiwell Plates for cell culture purposes can be found on page 11.
30 PAA – The Cell Culture Company | www.paa.com
Growth Area
(cm²)
Working
Volume (ml)
Pieces /
Bag
Purchase Unit
(pcs)
Molecular Biology
Tray Plates are suitable for a multitude of applications. PAA provides a tray plate that can be used in microbiology.
The rectangular shape of the tray dish provides a larger surface
area compared to round type dishes and thus enables easy
handling for any application. The external dimension of this tray
plate is identical to standard multiwell plates and thus allows
using standard HTS systems for automated application.
Cell Culture
Tray Plate
External
Dimension (mm)
Internal
Dimension (mm)
Growth
Area (cm2)
Sterilized
Pieces /
Bag
Purchase
Unit (pcs)
PS
127.6 x 85.6 x 16.2
109.5 x 73.5 x 11.6
80
+
10
100
Square Dishes
Square dishes are commonly used in microbiology applications.
They are designed for phage typing, agar diffusion assays, susceptibility testing, plate counts as well as traced colony picking.
The large surface area and the gridded bottom are useful for
tracing the location of individual colonies. PAA offers square
dishes made of polystyrene. The grid consists of thirty-six
squares.
Cat. No.
PAA11125X
PAA10245X
PAA11245X
Cell Imaging
> Optically clear
> High plate planarity
> Available with grids for cloning or determination of
plating efficiency
> Alphanumerical coding
> Sterilized
Immunoplates
PAA31001X
Material
Material
External
Dimension (mm)
Square Size (mm)
Growth
Area (cm2)
Sterilized
Pieces /
Bag
Purchase
Unit (pcs)
PS
PS
PS
125 x 125 x 20
245 x 245 x 20
245 x 245 x 28
18 x 18
35 x 35
35 x 35
139
500
500
+
+
+
10
5
5
240
20
20
PAA – The Cell Culture Company | www.paa.com 31
Handling & Storage
Cat. No.
Molecular Biology
> Clear grade polystyrene
> Useful for colony counting or colony picking
> Sterilized
Molecular Biology
Erlenmeyer flasks are one of the most commonly used tools for
all purposes in laboratories, e.g. pH titrations, shaker culture
applications such as microbial or plant cultures, as well as liquid
handling and storage. They feature a conical flat broad bottom
and a cylindrical neck.
PAA offers Erlenmeyer flasks suitable for many types of suspension cultures. They are made of autoclavable polycarbonate. The
flasks have a clear graduation on the side to indicate the approximate volume of the content. They are provided with a plastic
cap and available in the sizes of 250 ml, 500 ml and 1000 ml.
> Appropriate for shaker culture applications, liquid handling
or storage
> Suitable for many types of suspension cultures
(E. coli, plant callus, etc.)
> Half way closing enables air ventilation;
full closing seals tightly
> Breakage resistant
> Autoclavable
Immunoplates
Molecular Biology
Cell Culture
Erlenmeyer Flasks
PAA73250
PAA73250X
PAA73500
PAA73500X
PAA73000
PAA73000X
Handling & Storage
Cell Imaging
Cat. No.
32 PAA – The Cell Culture Company | www.paa.com
Material (Flask / Cap)
Volume (ml)
Purchase Unit (pcs)
PC / PP
PC / PP
PC / PP
PC / PP
PC / PP
PC / PP
250
250
500
500
1000
1000
1
8
1
8
1
4
Molecular Biology
es. Made of flexible polypropylene, the smooth loop surface
allows easy streaking as well as easy handling for inoculation.
Sterilization of the loops & needles reduces the contamination
risk and renders flaming unnecessary. This saves valuable time
for waiting of glass or metal instruments to cool down.
>
>
>
>
>
Cat. No.
Material
Inoculation Volume (µl)
Length (mm)
Sterilized
Pieces / Bag
Purchase Unit (pcs)
PP
PP
1
10
195
195
+
+
10
10
500
500
Immunoplates
PAA90001X
PAA90010X
For inoculation of microbes
Dual purpose: with loop and needle
Choice of inoculation volume (1 or 10 µl)
Flexible PP material
Sterilized
Molecular Biology
Loops & needles are convenient tools for picking isolated colonies within mixed cultures growing on plated medium. They are
also highly suitable for making stab inoculations into agar slants
or tubes with solid culture medium.
PAA provides two different sizes of loops for individual purpos-
Spreader
5 mm
Cat. No.
PAA90050X
>
>
>
>
>
Cell Imaging
140 mm
Sterilization of the spreader reduces the contamination risk and
renders flaming unnecessary. This saves valuable time for waiting of glass or metal instruments to cool down. The resistant
material enables autoclaving and reuse.
Breakage resistant
Immediate use without flaming
Spreading of microbe sample at petri dishes
Autoclavable
Sterilized
Material
Sterilized
Pieces / Bag
Purchase Unit (pcs)
PP
+
10
500
PAA – The Cell Culture Company | www.paa.com 33
Handling & Storage
PAA provides a convenient and cost saving cell spreader made
of polypropylene for different purposes in mammalian and bacterial culture work. The L-shaped design provides fast and even
sample distribution in culture dishes. Unlike glass alternatives,
plastic spreaders are non-breakable and do not pose a safety
hazard.
30 mm
Cell Culture
Loops & Needles
Colorimetric techniques, such as ELISA or general binding assays are used as a diagnostic tool
as well as a quality control check. PAA provides different types of immunoplates for qualitative and quantitative analyses. They feature a maxi-, medi- or unibinding surface to meet
every demand. The maxibinding surface is modified to provide a high binding capacity for
large molecules with both hydrophobic and hydrophilic regions, such as immunoglobulins
(antibody ELISA). The medibinding surface is well suited for smaller molecules with hydrophilic and hydrophobic properties (antibody / antigen ELISA). The unibinding surface is
designed for binding molecules with mainly hydrophobic regions (antigen ELISA).
34 PAA – The Cell Culture Company | www.paa.com
Immunoplates
96 Well Immunoplates (ELISA Plates) . . . . . . . . . . . . .
Strip Immunoplates . . . . . . . . . . . . . . . . . . . . . . . . . . .
Black & White Immunoplates . . . . . . . . . . . . . . . . . . .
384 Well Microplates . . . . . . . . . . . . . . . . . . . . . . . . .
36
36
37
37
PAA – The Cell Culture Company | www.paa.com 35
Immunoplates
Immunoplates are used in diagnostics and immunological
research. They are suitable for colorimetric techniques, such as
ELISA (Enzyme-linked Immunosorbent Assay) and provide
reliable performance in binding assays when consistent coating
of wells is required.
Cat. No.
Cell Imaging
Immunoplates
PAA32296X
PAA32396X
Handling & Storage
PAA offers ELISA plates with a maxi- or medibinding surface,
resulting in high binding capacity for proteins and other molecules with both hydrophilic and hydrophobic regions. The
arrangement of each well standing on its own and raised rims
allow safe sample handling. Therefore, the risk of contamination
from sample material being carried over is minimized. An alphanumeric coding ensures easy sample identification.
> Developed for immuno assays (ELISA)
and general binding assays
> Flat bottom type enables precise optical measurements
> Alphanumerical coding
> Designed for easy handling and automation
> Available with maxi- or medibinding surface
Molecular Biology
Cell Culture
96 Well Immunoplates (ELISA Plates)
Material
Well Format
Surface Type
External Dimension
(mm)
Total Volume
(µl)
Pieces /
Bag
Purchase Unit
(pcs)
PS
PS
96 F
96 F
Maxibinding
Medibinding
85.4 x 127.6
85.4 x 127.6
400
400
10
10
100
100
Strip Immunoplates
For greater flexibility in diagnostics PAA offers strip immunoplates with maxi- or medibinding surface. One strip consists of
8 wells and 12 strips fit into one frame, thus forming a standard
96 well plate.
The wells of PAA38096X and PAA38196X are stabilized with a
polystyrene bar and thus fixed for easy and safe handling.
With PAA38296X and PAA38396X the individual wells can be
easily broken off separately. The number of performing tests can
thus be precisely adjusted to the number of samples. The frame
itself provides individual 96 wells for safe fixation of the wells.
An alphanumerical coding ensures easy sample identification for
both products.
> Developed for immuno assays (ELISA)
and general binding assays
> Flat bottom type enables precise optical measurements
> Raised rims on the wells
> Alphanumerical coding
> Designed for easy handling and automation
> Available with maxi- or medibinding surface
Strip Type
Well Type
Cat. No.
PAA38096X
PAA38196X
PAA38296X
PAA38396X
Material
Configuration
Surface Type
External Dimension
(mm)
Total Volume
(µl)
Pieces /
Bag
Purchase Unit
(pcs)
PS
PS
PS
PS
Strip Type
Strip Type
Well Type
Well Type
Maxibinding
Medibinding
Maxibinding
Medibinding
85.4 x 127.6
85.4 x 127.6
85.4 x 127.6
85.4 x 127.6
400
400
400
400
10
10
10
10
100
100
100
100
36 PAA – The Cell Culture Company | www.paa.com
Immunoplates
PAA provides black and white high quality 96 well immunoplates suitable for various high throughput assays. High signalto-noise ratio with low background is a characteristic feature of
these plates.
The surface of the plates is modified with both hydrophilic and
hydrophobic regions, thus showing a maximum binding capacity.
Well Format
Plate / Lid
Surface Type
PS
PS
96 F
96 F
White / Trans.
Black / Black
Maxibinding
Maxibinding
External Dimension Total Volume
(mm)
(µl)
85.4 x 127.6
85.4 x 127.6
400
400
Pieces /
Bag
Purchase Unit
(pcs)
10
10
100
100
384 Well Immunoplates
High throughput assays and automated systems are used for the
economic handling of a huge number of samples. To minimize
the working volume, 384 well microplates are recommended.
PAA offers high quality 384 well imunooplates in transparent,
black or white, each with a transparent lid. High signal-to-noise
ratio with low background is a characteristic feature of these
plates. Alphanumerical well coding ensures easy sample identification. The plates are available with maxibinding surface for
molecules with both hydrophobic and hydrophilic properties as
well as with unibinding surface for rather hydrophobic molecules.
>
>
>
>
>
>
>
Cat. No.
PAA31384X
PAA32384X
PAA33384X
PAA34384X
PAA35384X
PAA36384X
HTS compatible
For colorimetric, fluorescence and luminescence assays
Low Background
High signal-to-noise ratio
Transparent, black or white
Available with maxi- and unibinding surface
Provided with transparent lid
Material
Well Format
Plate / Lid
Surface Type
PS
PS
PS
PS
PS
PS
384 F
384 F
384 F
384 F
384 F
384 F
Clear / Trans.
White / Trans.
Black / Trans.
Clear / Trans.
White / Trans.
Black / Trans.
Unibinding
Unibinding
Unibinding
Maxibinding
Maxibinding
Maxibinding
External Dimension Total Volume
(mm)
(µl)
85.4 x 127.6
85.4 x 127.6
85.4 x 127.6
85.4 x 127.6
85.4 x 127.6
85.4 x 127.6
120
120
120
120
120
120
Pieces /
Bag
Purchase Unit
(pcs)
10
10
10
10
10
10
40
40
40
40
40
40
PAA – The Cell Culture Company | www.paa.com 37
Immunoplates
PAA31396X
PAA31496X
Material
Cell Imaging
Cat. No.
Molecular Biology
> Available in white and black
> HTS compatible
> Suitable for colorimetric assay, fluorescence assay,
luminescence assay
> High signal-to-noise ratio
> Low background interference
> Maxibinding surface for high binding capacity
> Provided with lid
Handling & Storage
Colorimetric assays, e.g. fluorescence or luminescence measurements are more and more common in cell culture research. To
avoid negative side effects such as background interference,
autofluorescence or cross talk the use of black or white pigmented microplates is recommended.
Cell Culture
Black & White Immunoplates (Fluoro- & Luminoplates)
Cell based imaging techniques show a rapid growth in different areas of research and development. The requirements are higher than for standard applications. PAA provides innovative products for cell based assays, fluorescence microscopy and high content ­screening. They
feature high plate planarity, low background ­interference and high light transmission for
challenging experiments.
38 PAA – The Cell Culture Company | www.paa.com
Cell Imaging
Imaging Plates CG, with Glass Bottom . . . . . . . . . . . .
Imaging Plates FC, with Fluorocarbon Film Bottom . . .
Imaging Dishes CG (Cover Glass) . . . . . . . . . . . . . . . .
Imaging Dish Stage Frames . . . . . . . . . . . . . . . . . . . . .
Imaging Chambers / Imaging Chambers CG . . . . . . . .
Imaging Chamber Accessories . . . . . . . . . . . . . . . . . .
Cell Culture Slides . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Slide Tray Plates . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
CytoCapture . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
41
42
43
43
44
45
46
47
48
PAA – The Cell Culture Company | www.paa.com 39
Cell Imaging
Molecular Biology
Cell Culture
Cell Imaging
Surface treatment by methods of micro- and nanopatterning
becomes more and more important in many applications of the
life science sector. PAA provides polymer and glass products
designed to support challenging cell based imaging and spectro-
Detection technologies
> Laser Scanning Microscopy
> Live Cell Imaging
> Differential Interference Contrast Microscopy
> Total Internal Reflection Microscopy
> Fluorescence Correlation Spectroscopy
> Slide based technologies, slide and array manipulation
Immunoplates
Special assays
> Intracellular imaging
> Control of gaseous partial pressure in cellular
microenvironment
> Phototoxicity studies (UVA and UVB)
> Horizontal manipulation and maintenance of microscopy
slides, array slides (e.g. Comet-Assay or hybridization
technologies)
Special cell culture requirements
> Cells with high energy and oxygen requirements
> Cells with sensitive adhesion
> Primary cells derived from solid tissues
Technology compatibility
Fluorescence Intensity
Fluorescence Correlation Spectroscopy*
Confocal Microscopy* and Spinning Disc Confocal Microscopy
Phase Contrast Microscopy
Differential Interference Contrast
Hoffmann's Modulation Contrast
Total Internal Reflection Microscopy
FRET, FRAP, FLIM, TRF, FP
Laser Scanning Cytometry
*Bottom flatness of high importance
PS plate
CG
FC
+
(+)
(+)
+
−
+
−
+/−
+
+
+
+
+
+
+
+
+
+
+
+
+
+
−
+
−
+
+
PS = Polystyrene
CG = Cover Glass
FC = Fluorocarbon
The pictures below show adherent cells cultivated in Imaging
Plates FC, stained and analyzed under the microscope. The gas
permeable highly light transmitting bottom material (fluorocarbon) enables a special combination of excellent imaging fea-
tures and support of cell physiology. Without the need for cover
slips outstanding results can be achieved in fluorescence analysis
and imaging directly from the multiwell plate.
Fluorescent Phalloidin-TRITC (red) marking F-actin filaments and
Hoechst 33342 stain (blue) marking the nucleus in HeLa cells (40x)
Phase contrast image of HeLa cells (40x)
Handling & Storage
Cell Imaging
metry tasks. General applications are among others: Cell migration, target identification, early and secondary toxicology, functional genomics and high throughput screening.
40 PAA – The Cell Culture Company | www.paa.com
Cell Imaging
Low evaporation lids reduce edge effects. The anticondensation
treatment of the lid minimizes condensation droplet formation.
The external dimensions are in compliance with the recommendations of the Society for Biomolecular Screening (SBS).
Therefore, the plates are suitable for most standard automated
microscopes and imaging readers.
Immunoplates
Molecular Biology
PAA offers black polymer plates (24 and 96 well) with a bottom
made of borosilicate glass. The extra thin glass bottom (145 µm)
combined with an excellent planarity allows imaging techniques
with high resolution. Advanced surface treatment ensures optimal cell adhesion, distribution and spreading.
Cell Culture
Imaging Plates CG, with Glass Bottom
Applications
> Total Internal Reflection Fluorescence (TIRF)
> Laser Scanning Confocal Microscopy
> Differential Interference Contrast (DIC)
> Fluorescence Correlation Spectroscopy (FCS)
Well
Format
Bottom
Material
Inner Well
Diameter (mm)
Suggested Working Volume (µl)
Total
Volume (µl)
Surface
Treated
Sterilized
Pieces /
Bag
Purchase
Unit (pcs)
PAA21315231
24
Borosilicate
glass
13.2
500 – 1000
1880
+
+
1
1
PAA21315231X
24
Borosilicate
glass
13.2
500 – 1000
1880
+
+
1
20
PAA21315241
96
Borosilicate
glass
6.0
100 – 200
428
+
+
1
1
PAA21315241X
96
Borosilicate
glass
6.0
100 – 200
428
+
+
1
20
Cat. No.
PAA – The Cell Culture Company | www.paa.com 41
Handling & Storage
Cell Imaging
Features
> Excellent plate planarity (inner well flatness ≤ 10 µm)
> Extra thin glass bottom (145 µm)
> Imaging with high resolution
> No further coating necessary
> Optimal cell adhesion
> No cross contamination due to anti-condensation
treatment of lid
> Temperature range −20 °C to +50 °C
> Sterile
> Single packaged
Cell Imaging
Due to a very low inner well flatness and well to well flatness
fluorocarbon imaging plates now open up new dimensions of
imaging techniques.
PAA offers black polymer plates (24 and 96 well) with a bottom
made of high performance fluorocarbon film. The bottom of the
plates is 25 µm thin. The fluorocarbon film is highly light transmitting and gas permeable thus combining excellent imaging
features and support of cell physiology. Advanced surface treatment ensures optimal cell adhesion, distribution and spreading.
The 96 well version is designed as low profile plate to support
the use of immersion objectives for the entire plate. In addition,
low evaporation lids reduce edge effects. The anticondensation
treatment of the lid minimizes droplet formation.
The external dimensions are in compliance with the recommendations of the Society for Biomolecular Screening (SBS) and
therefore suitable for the majority of standard automated microscopes and imaging readers.
Immunoplates
Molecular Biology
Cell Culture
Imaging Plates FC, with Fluorocarbon Film Bottom
Handling & Storage
Cell Imaging
Applications
> Highly metabolically active cells
> Life cell imaging
> High resolution imaging
> Sensitive fluorescence dyes
Features
> Excellent plate planarity (inner well flatness ≤ 10 µm)
> Extra thin gas permeable bottom (25 µm)
> High performance fluorocarbon film bottom
> Excellent light transmission
> Low intrinsic fluorescence
> No further coating necessary
> Temperature range −20 °C to +50 °C
> Sterile
> Single packaged
Cat. No.
PAA21313231
PAA21313231X
PAA21313241
PAA21313241X
Well
Format
Bottom
Material
24
24
96
96
Fluorocarbon
Fluorocarbon
Fluorocarbon
Fluorocarbon
Inner Well
Suggested Working
Total
Surface
Pieces /
Sterilized
Diameter (mm)
Volume (µl)
Volume (µl) Treated
Bag
42 PAA – The Cell Culture Company | www.paa.com
13.2
13.2
6.0
6.0
500 – 1000
500 – 1000
100 – 200
100 – 200
1880
1880
428
428
+
+
+
+
+
+
+
+
1
1
1
1
Purchase
Unit (pcs)
1
20
1
20
Cell Imaging
easy positioning of the dish and (re-)localisation of the region of
interest if combined with our imaging dish stage frames. These
are available in two variants: as single dish frame that holds one
dish and can be used on a plane microscope or with inserts for
slides and as double dish frame that can hold two imaging
dishes. A stage insert for multiwell plates is necessary to use this
variant. The dishes are packed in bags of six with a tray of polystyrene which can be used for better and safer transport of the
dishes in the laboratory.
Applications
> Laser Scanning Confocal Microscopy
> Total Internal Reflection Fluorescence (TIRF)
> Differential Interference Contrast (DIC)
> Fluorescence Correlation Spectroscopy (FCS)
> Low Intensity Fluorescence
Features
> Extra thin glass bottom
> Imaging with high resolution
> Surface treatment to support
cell spreading and adhesion
> Easy grip for secure handling
> Imaging area Ø: 18 mm
> Temperature range -20°C to +50°C
> Packaged in bags of six with a tray
Cell Imaging
Immunoplates
Molecular Biology
PAA offers imaging dishes with a bottom made of cover glass
for imaging purposes which are highly demanding. The material
of this bottom is borosilicate glass and provides an excellent
planarity and focus control for imaging techniques with high
resolution. The 35 mm dishes are available with 145 µm or
175 µm glass thickness. For better microscopic orientation the
175 µm glass version is also offered with engraved micro grid.
Macroscopic orientation is supported by N-O-S-W marks. A grip
zone around the dish allows secure handling and enables an
Cell Culture
Imaging Dishes CG (Cover Glass) & Stage Frames
Cat. No.
PAA12305160
PAA12305160X
PAA12305161X
PAA12306160
PAA12306160X
PAA12306161X
PAA12307160
PAA12307160X
PAA12307161X
Glass Thickness (µm)
µGrid
Sterilized
Pieces / Bag
Purchase Unit (pcs)
145
145
145
175
175
175
175
175
175
−
–
−
−
−
−
+
+
+
+
+
+
+
+
+
+
+
+
6
6
6
6
6
6
6
6
6
6
30
168
6
30
168
6
30
168
Frame Type
Outer Dimensions (mm)
Sterilized
Pieces / Bag
Purchase Unit (pcs)
Single
Double
52 x 76
127.76 x 85.48
–
–
1
1
1
1
Imaging Dish Stage Frame
Cat. No.
PAA12300001
PAA12300002
PAA – The Cell Culture Company | www.paa.com 43
Handling & Storage
Imaging Dishes CG
Cell Imaging
For convenient handling PAA offers Imaging Chambers that
allow culturing, staining and fixation of cells inside the same
vessel. They are available with cover glass (CG) or microscope
slides as bottom structure. Both types are available in 1 well,
2 well, 4 well and 8 well format. Imaging Chambers CG are
specially designed for high resolution live cell microscopy techniques. Imaging Chamber with microscope slides can be used
with long distance objectives.
PAA`s Imaging Chambers and lids are made of high performance polymers with better chemical resistance and temperature
stability than the commonly used polystyrene. The chambers are
sealed against the glass bottoms by biologically inert silicone
adhesive. At the end of the experiments the glasses can be
easily detached from the polymer chambers with minimal glue
residue.
Immunoplates
Molecular Biology
Cell Culture
Imaging Chambers / Imaging Chambers CG
Applications
> Immunohistology
> Total Internal Reflection Fluorescence (TIRF)
> Fluorescence Resonance Energy Transfer (FRET)
> Differential Interference Contrast (DIC)
> Fluorescence In-Situ Hybridization (FISH)
> Long-term storage
Microscope Slide
Handling & Storage
Cell Imaging
Cover Glass
Cat. No.
PAA14220010X
PAA14220011X
PAA14220020X
PAA14220021X
PAA14220040X
PAA14220041X
PAA14220080X
PAA14220081X
PAA14110010X
PAA14110011X
PAA14110020X
PAA14110021X
PAA14110040X
PAA14110041X
PAA14110080X
PAA14110081X
Features
> Easy removal of growth chamber
> Biologically inert glue
> Great chemical resistance (e.g. acetone)
> High temperature stability up to +90 °C
> Outstanding cell adhesion due to plasma treatment
Format
Bottom Type
1 well
Cover Glass
10.84
2.0
+
4
2 well
Cover Glass
5.04
1.0
+
4
4 well
Cover Glass
2.14
0.5
+
4
8 well
Cover Glass
0.88
0.3
+
4
1 well
Microscope Slide
10.84
2.0
+
4
2 well
Microscope Slide
5.04
1.0
+
4
4 well
Microscope Slide
2.14
0.5
+
4
8 well
Microscope Slide
0.88
0.3
+
4
44 PAA – The Cell Culture Company | www.paa.com
Growth Area (cm2) Working Volume (ml) Sterilized Pieces / Bag Purchase Unit (pcs)
16
80
16
80
16
80
16
80
16
80
16
80
16
80
16
80
Cell Imaging
For the extension of the Imaging Chambers CG to full length of
standard microscope slides PAA offers an optional adapter. This
allows the usage of Imaging Chambers CG with regular microscope stage inserts for microscope slides. The adapter is made
of stainless steel and can be reused.
Imaging Chamber DIC Lid
Imaging Chamber Adapter
Sterilized
Pieces / Bag
+
–
4
1
Purchase Unit (pcs)
4
1
Cell Imaging
Produkt
PAA14220001
PAA14220002
Handling & Storage
Cat. No.
Immunoplates
Molecular Biology
PAA provides a special lid with borosilicate glass insert as alternative to the standard polymer lid in order to improve the results
during differential interference contrast (DIC) microscopy. The
lids are sterilized and packed in bags of four. Cell Culture
Imaging Chamber Accessories
PAA – The Cell Culture Company | www.paa.com 45
Cell Imaging
PAA offers 4 well and 8 well cell culture slides that enable culturing, staining and fixation of cells in the same vessel. The chambers can easily be detached from the microscope slide bottom
without any glue residues. In addition to cell culture slides with
a transparent chamber, they are also available with black or
white chamber for colorimetric assays. The cell culture slides are
packed in trays of six pieces, sealed individually. Thus, sterility is
preserved when only a single slide is needed.
Cell Imaging
Immunoplates
Molecular Biology
Cell Culture
Cell Culture Slides
Features
> Easy separation of chamber and slide
> No glue residues
> Leak-proof
> Black or white chamber for colorimetric assays
> Sterilized
> Non-pyrogenic
Cat. No.
Chamber Color
Growth Area
(cm2)
Working
Volume (ml)
Sterilized
Pieces /
Bag
Purchase
Unit (pcs)
4 well
4 well
4 well
8 well
8 well
8 well
Clear
Black
White
Clear
Black
White
2.13
2.13
2.13
0.98
0.98
0.98
0.5 – 1.3
0.5 – 1.3
0.5 – 1.3
0.2 – 0.6
0.2 – 0.6
0.2 – 0.6
+
+
+
+
+
+
6
6
6
6
6
6
12
12
12
12
12
12
Handling & Storage
PAA30104X
PAA30114X
PAA30124X
PAA30108X
PAA30118X
PAA30128X
Chamber
Format
46 PAA – The Cell Culture Company | www.paa.com
Cell Imaging
site for the cultivation of cells. Polypropylene (PP) on the other
hand features a better chemical resistance towards reagents
such as acetone. The plates can handle up to four slides in parallel.
PAA11200145
PAA11200145X
PAA11201145
PAA11201145X
PAA11400145
PAA11400145X
Material
Single Chamber
Width / Length (mm)
Surface Treated
Sterilized
Pieces / Bag
Purchase Unit (pcs)
PS
PS
PS
PS
PP
PP
28 x 78
28 x 78
28 x 78
28 x 78
28 x 78
28 x 78
−
−
+
+
−
−
+
+
+
+
−
−
10
10
10
10
10
10
10
40
10
40
10
40
Innovative product line for Cytogenetics
Cytogenetics is the study of chromosomes and the related diseases caused by
numerical and structural chromosome abnormalities. Chromosome studies are
important diagnostic procedures in prenatal and postnatal diagnosis. PAA offers a
product line including special media, serum and reagents for the cultivation of
amniocytes, chorionic villi cells and lymphocytes. These products support you to
achieve reliable results for further medical interventions. Read more about PAA´s
Cytogenetic Products at
www.paa.com/cell_culture_products/cytogenetics.html
PAA – The Cell Culture Company | www.paa.com 47
Cell Imaging
Cat. No.
Easy handling
Easy access zone for slide removal
Transparent PS or chemical resistant PP
For most standard slides
Parallel testing
Stackable
Handling & Storage
>
>
>
>
>
>
Immunoplates
Molecular Biology
Slide Tray Plates are a convenient solution if you have to handle
your microscope slides in horizontal orientation, e.g. for cell
staining, chromosome preparation or Comet-Assay analysis. The
PAA slide tray plates are available in two different materials.
Polystyrene (PS) is crystal clear and sterile which is the prerequi-
Cell Culture
Slide Tray Plates
Cell Imaging
It is very challenging to trace non adherent cells under the
microscope due to the prevailing fluid dynamics. For experiments that require the separation and arrest of living cells or
spheroids PAA offers a variety of microscopy labware with
microscale cavities. These CytoCapture structures promote the
spontaneous sedimentation of cells and protect them against
fluid movements. Media exchange as well as staining or fixation
can be performed while the cells reside within their cavities.
CytoCapture products are also well-suitable for 3D cultivation
and imaging of individual adherent cells.
Cell Imaging
Immunoplates
Molecular Biology
Cell Culture
CytoCapture
Imaging Dish CG
Microscope Slide
Applications
> Time lapse analysis of suspension cells
> Life cell staining
> Culture and analysis of spheroids
> 3D cultivation of adherent cells
> Immunohistochemistry
Features
> Spontaneous sedimentation of cells in single cavities
> Maintenance of cell location during treatments
> Well selected glass bottoms for high resolution microscopy
Available combinations of cavity geometry and labware format:
Labware Format
Imaging Dish CG
Microscope Slide
Imaging Chamber
Small hexagonal
+
+
+
Square
+
+
+
Big hexagonal
+
–
–
Big hexagonal
Low cell adhesion
+
–
+
Cavity geometry
Handling & Storage
Imaging Chamber
Cavity geometry
Best suitable for:
Small hexagonal
Capturing suspension cells with a diameter below 15 µm or cell nuclei
Square
Capturing larger suspension cells and for 3D guided cultivation of adherent cells
Big hexagonal
Clustering up to 100 adherent or suspension cells per microcavity
Big hexagonal
Low cell adhesion
Controlled formation and investigation of cellular spheroids
CytoCapture is a joint development of Molecular Cytomics Inc. and zell-kontakt GmbH.
48 PAA – The Cell Culture Company | www.paa.com
Cell Imaging
such as media exchange, life staining of cells or intracellular
structures and immunohistochemistry. The single cells can be
monitored at different time intervals to investigate the response
to specific experimental procedures.
>
>
>
>
Cat. No.
PAA17116600
PAA17116600X
PAA17117700
PAA17117700X
PAA17118800
PAA17118800X
Hexagonal structure for capturing single cells
Available in three different labware types
Suitable for suspension cells < 15 µm
Cavity size:
20 µm diameter, 10 µm depth, 3 µm wall thickness
Labware
Type
Cavity
Geometry
Cavity Width
(µm)
Working Volume
Low Cell
Adhesion
Sterilized
Pieces /
Bag
Purchase Unit
(pcs)
Dish
Dish
Slide
Slide
Chamber
Chamber
Hexagonal
Hexagonal
Hexagonal
Hexagonal
Hexagonal
Hexagonal
Ø 20
Ø 20
Ø 20
Ø 20
Ø 20
Ø 20
50 µl (1.5 ml)*
50 µl (1.5 ml)*
10 µl
10 µl
300 µl
300 µl
–
–
–
–
–
–
+
+
+
+
+
+
1
1
4
4
4
4
1
10
4
20
4
16
* Seed cells in 50 µl and fill up to 1.5 ml.
PAA – The Cell Culture Company | www.paa.com 49
Handling & Storage
30 µm
Cell Imaging
Immunoplates
Molecular Biology
For convenient imaging of suspension cells PAA offers
CytoCapture products with small hexagonal microscale cavities.
This geometry is best suitable for trapping small individual cells
and protecting them against fluid movements during treatments
Cell Culture
CytoCapture: Small Hexagonal Cavities
Cell Imaging
For the capture and imaging of larger single cells PAA offers
CytoCapture products with square cavities. This type of geometry is especially designed for 3D guided cultivation of adherent
cells (e.g. epithelial cells). The upright growth of the cells allows
a more natural cell development with both apical and basolateral membrane sides. The microstructure assisted control of cell
morphology is a great basis for complex cell-cell interaction
studies or co-culture experiments.
Immunoplates
Molecular Biology
Cell Culture
CytoCapture: Square Cavities
Cell Imaging
>
>
>
>
Handling & Storage
Cat. No.
PAA17226600
PAA17226600X
PAA17227700
PAA17227700X
PAA17228800
PAA17228800X
Square structure for capturing single cells
Available in three different labware types
Suitable for adherent and suspension cells
Cavity size: 40 µm side length, 15 µm depth, 3 µm wall thickness
Labware
Type
Cavity
Geometry
Cavity Width
(µm)
Working Volume
Low Cell
Adhesion
Sterilized
Pieces /
Bag
Purchase Unit
(pcs)
Dish
Dish
Slide
Slide
Chamber
Chamber
Square
Square
Square
Square
Square
Square
40 x 40
40 x 40
40 x 40
40 x 40
40 x 40
40 x 40
50 µl (1.5 ml)*
50 µl (1.5 ml)*
10 µl
10 µl
300 µl
300 µl
–
–
–
–
–
–
+
+
+
+
+
+
1
1
4
4
4
4
1
10
4
20
4
16
* Seed cells in 50 µl and fill up to 1.5 ml.
50 PAA – The Cell Culture Company | www.paa.com
Cell Imaging
aggregate and spheroid formation even of adherent cells.
With the CytoCapture products it is possible to change media,
add substances and manipulate cells or spheroids without interference in their location. Thus, the region of interest can be kept
under control allowing also time dependent experiments.
Cat. No.
PAA17336600
PAA17336600X
PAA17446600
PAA17446600X
PAA17448800
PAA17448800X
PAA17556600
PAA17556600X
PAA17558800
PAA17558800X
Labware
Type
Cavity
Geometry
Cavity Width
(µm)
Working Volume
Low Cell
Adhesion
Sterilized
Pieces /
Bag
Purchase Unit
(pcs)
Dish
Dish
Dish
Dish
Chamber
Chamber
Dish
Dish
Chamber
Chamber
Hexagonal
Hexagonal
Hexagonal
Hexagonal
Hexagonal
Hexagonal
Hexagonal
Hexagonal
Hexagonal
Hexagonal
Ø 250
Ø 250
Ø 250
Ø 250
Ø 250
Ø 250
Ø 500
Ø 500
Ø 500
Ø 500
50 µl (1.5 ml)*
50 µl (1.5 ml)*
50 µl (1.5 ml)*
50 µl (1.5 ml)*
300 µl
300 µl
50 µl (1.5 ml)*
50 µl (1.5 ml)*
300 µl
300 µl
–
–
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
1
1
1
1
4
4
1
1
4
4
1
10
1
10
4
16
1
10
4
16
* Seed cells in 50 µl and fill up to 1.5 ml.
PAA – The Cell Culture Company | www.paa.com 51
Cell Imaging
Hexagonal structure for capturing up to 100 cells
Suitable for adherent and suspension cells
Spontaneous sedimentation and clustering of cells in cavities
Low adhesion surface especially for spheroid cultivation
Enables uniform formation of embryoid bodies
Cavity sizes: 250 µm diameter, 100 µm depth, 5 µm wall thickness
500 µm diameter, 100 µm depth, 10 µm wall thickness
Handling & Storage
>
>
>
>
>
>
Immunoplates
Molecular Biology
Investigation of cell-cell interactions between suspension cells is
a particular challenge. PAA offers CytoCapture products with
big hexagonal cavities to facilitate experiments where a controlled
clustering and analysis of cells is required. These are also available
with low adhesion surface, which contributes to an enforced
Cell Culture
CytoCapture: Big Hexagonal Cavities
DNA, RNA and protein containing material must be properly handled and stored. The same
goes for freezing of mammalian cells and their long term storage in liquid nitrogen. Therefore,
PAA ­provides high quality racks and storage boxes also for use in cryo conservation to meet
different kinds of applications.
52 PAA – The Cell Culture Company | www.paa.com
Handling & Storage
Cryotubes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Inserts for Cryotubes . . . . . . . . . . . . . . . . . . . . . . . . . .
Working Rack for Cryotubes . . . . . . . . . . . . . . . . . . . .
Cryo Boxes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Storage Boxes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Storage Boxes with Hinged Cover . . . . . . . . . . . . . . . .
Conical Centrifuge Tube Racks . . . . . . . . . . . . . . . . . .
Microtube Racks . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
PCR Tube Racks . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Reservoirs . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Square Bottles . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Autoclave Bags . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
54
55
55
56
57
58
59
60
61
62
63
64
PAA – The Cell Culture Company | www.paa.com 53
Handling & Storage
Cryotubes
>
>
>
>
>
Safe storage of biological material
White printed graduation and large writing area
Colored inserts available for easy sample identification
External thread
Easy to handle in one-hand operation
within the working rack
> Free of DNase / RNase
> Sterile
> Non-pyrogenic
Cat. No.
PAA535001
PAA535001X
PAA535002
PAA535002X
Material
Volume (ml)
Pieces / Bag
Purchase Unit (pcs)
PP
PP
PP
PP
1.2
1.2
2.0
2.0
100
100
100
100
100
1000
100
1000
Handling & Storage
Cell Imaging
Immunoplates
Molecular Biology
Cell Culture
Cryotubes are commonly used for storage of cells, sera, blood
components, bacteriological cultures and other fluids at low
temperature.
The cryotubes of PAA are made of clear high grade polypropylene. Using the same material for both vial and lid as well as the
exclusion of an O-ring reduce the risk of explosion during thawing to a minimum. The round bottom shape facilitates complete
transfer of the cell material to the cell culture flask or dish. The
starfoot base of the tubes enables easy handling within a working rack. The cryotubes are suitable for storage in refrigerators
or in the gas phase of liquid nitrogen as the material is stable at
temperatures down to -196 °C.
Excellent cooling and freezing conditions with PAA’s Cell Preservation Products
There are two common methods of cell preservation. Hypothermic preservation at temperatures above freezing allows short term storage of tissues and organs. Cryogenic
preservation at temperatures below freezing is used for the establishment of cell banks
to ensure reproducible results in research by using stable cell strains. All PAA cell preservation products provide outstanding cooling and freezing conditions with maximum viability and recovery after warming. Learn more about PAA´s Cell Preservation Products at
www.paa.com/cell_culture_products/cellpreservation.html
54 PAA – The Cell Culture Company | www.paa.com
Handling & Storage
PAA535050
PAA535050B
PAA535050W
PAA535050Y
PAA535050V
PAA535050R
Color
5 colors
(Blue, White, Yellow, Violet, Red)
Blue
White
Yellow
Violet
Red
Material
Pieces / Bag
Purchase Unit (pcs)
PP
100
5 x 100
PP
PP
PP
PP
PP
100
100
100
100
100
100
100
100
100
100
Working Rack for Cryotubes
Molecular Biology
Cat. No.
Immunoplates
For easy sample identification PAA provides fitting cap inserts in
five different colors.
Cell Culture
Inserts for Cryotubes
Cat. No.
PAA535040
Material
Array (holes)
Purchase Unit (pcs)
PC
4 x 10
1
PAA – The Cell Culture Company | www.paa.com 55
Handling & Storage
Cell Imaging
PAA offers an appropriate support rack suitable for cryotubes
with a starfoot base. This system eases the handling of the tubes
with one hand. The rack provides space for up to 40 cryotubes.
Handling & Storage
The cryo boxes are made of clear polycarbonate. They are
designed for the handling and storage of 1.2 ml and 2 ml cryotubes to be used at temperatures ranging from -196 °C to
+121 °C. The numbering on the transparent lid of the box
allows a quick and easy inventory control. The boxes are available in three different colors (yellow, blue, red) and in two different sizes (25 and 81 positions).
Immunoplates
Molecular Biology
Cell Culture
Cryo Boxes
Temperature range: -196 °C to +121 °C
Enhanced draw off of liquid nitrogen
Clear numbered lid
Two different sizes
Three different colors
Material: clear polycarbonate (PC)
Cell Imaging
>
>
>
>
>
>
Handling & Storage
Cat. No.
PAA80025Y
PAA80025B
PAA80025R
PAA80081Y
PAA80081B
PAA80081R
Color
Material
Yellow
Blue
Red
Yellow
Blue
Red
PC
PC
PC
PC
PC
PC
56 PAA – The Cell Culture Company | www.paa.com
External Dimension (mm)
76 x 76 x 53
76 x 76 x 53
76 x 76 x 53
132 x 132 x 53
132 x 132 x 53
132 x 132 x 53
Array (holes)
Purchase Unit (pcs)
5x5
5x5
5x5
9x9
9x9
9x9
1
1
1
1
1
1
Handling & Storage
They are intended for use in a temperature range of -80 °C to
+121 °C. The storage boxes are available with different lids and
colors.
Molecular Biology
The PAA storage boxes are made of polypropylene and prove to
be an excellent option for storage and handling of 1.2 ml and
2 ml cryotubes and 1.5 ml microtubes in the laboratory.
Cell Culture
Storage Boxes
Storage Box with alphanumerical coding on lid and bottom
For the storage of 1.2 and 2 ml cryotubes, 1.5 ml microtubes
PAA535055B
PAA535055G
PAA535055R
PAA535055Y
PAA535055T
Color
Material
External Dimension (mm)
Array (holes)
Purchase Unit (pcs)
Blue
Green
Red
Yellow
Transparent
PP
PP
PP
PP
PP
130 x 130 x 54
130 x 130 x 54
130 x 130 x 54
130 x 130 x 54
130 x 130 x 54
9x9
9x9
9x9
9x9
9x9
1
1
1
1
1
Cell Imaging
Cat. No.
Immunoplates
> Temperature range -80 °C to +121 °C
> For 1.2 ml and 2 ml cryotubes, 1.5 ml microtubes
> Different colors available
Storage Box with numerical coding on inlay
For the storage of 1.2 ml and 2 ml cryotubes, 1.5 ml microtubes
Cat. No.
PAA81081
PAA81081X
Color
Material
External Dimension (mm)
Array (holes)
Purchase Unit (pcs)
Transparent
Transparent
PP
PP
130 x 130 x 51
130 x 130 x 51
9x9
9x9
1
40
PAA – The Cell Culture Company | www.paa.com 57
Handling & Storage
> Temperature range -80°C to +121 °C
> For 1.2 ml and 2 ml cryotubes, 1.5 ml microtubes
Handling & Storage
The PAA storage boxes with hinged cover are made of polypropylene and prove to be an excellent option for storage and
handling of 1.2 ml cryotubes and 1.5 ml microtubes in the labo-
ratory. They are intended for use in a temperature range of
-80 °C to +121 °C. The storage boxes are available with different lids and colors. The boxes can be sterilized by autoclaving.
Storage Box with alphanumerical coding on hinged cover
For the storage of 1.2 ml cryotubes and 1.5 ml microtubes
>
>
>
>
Cat. No.
PAA80100
PAA80100X
PAA81100G
PAA81100V
PAA81100O
PAA81100P
PAA81100B
Temperature range -80 °C to +121 °C
Autoclavable
For 1.2 ml cryotubes and 1.5 ml microtubes
Different colors available
Color
Material
External Dimension (mm)
Array (holes)
Purchase Unit (pcs)
Transparent
Transparent
Green
Violet
Orange
Pink
Blue
PP
PP
PP
PP
PP
PP
PP
130 x 146 x 55
130 x 146 x 55
130 x 146 x 55
130 x 146 x 55
130 x 146 x 55
130 x 146 x 55
130 x 146 x 55
10 x 10
10 x 10
10 x 10
10 x 10
10 x 10
10 x 10
10 x 10
1
30
1
1
1
1
1
Handling & Storage
Cell Imaging
Immunoplates
Molecular Biology
Cell Culture
Storage Boxes with Hinged Cover
58 PAA – The Cell Culture Company | www.paa.com
Handling & Storage
50 ml). The folded racks are a cost saving alternative to stainless
wire racks or other plastic racks. They are easy to assemble and
made of polypropylene.
Handling and storage of 25 conical tubes
Special position circles ensure safe stand
Autoclavable
Three colors available
Color
Material
Tube Size (ml)
Description
Purchase Unit (pcs)
PAA52015R
PAA52015B
PAA52015G
PAA52050R
PAA52050B
PAA52050G
Red
Blue
Green
Red
Blue
Green
PP
PP
PP
PP
PP
PP
15
15
15
40, 50
40, 50
40, 50
25 holes
25 holes
25 holes
25 holes
25 holes
25 holes
1
1
1
1
1
1
Handling & Storage
Cat. No.
Cell Imaging
>
>
>
>
Immunoplates
Molecular Biology
In the daily working routine conical centrifuge tubes are often
used in the laboratory. PAA offers conical tube racks that are
suitable for handling and storage of 25 conical tubes (15, 40 or
Cell Culture
Conical Centrifuge Tube Racks
PAA – The Cell Culture Company | www.paa.com 59
Handling & Storage
Microtube racks are excellent tools for the preparation of samples. PAA offers two different types of racks. The classical one is
made for handling and storage of up to 80 microtubes. The
double sided one alternatively provides space for 0.2 ml microtubes. Both racks are provided with a numerical coding for easy
tube identification.
Immunoplates
Molecular Biology
Cell Culture
Microtube Racks
> For handling and storage of up to 80 microtubes
> 5 different colors
Cat. No.
Color
Material
Array (holes)
Purchase Unit (pcs)
PAA61080W
PAA61080R
PAA61080B
PAA61080G
PAA61080V
White
Red
Blue
Green
Violet
PP
PP
PP
PP
PP
5 x 16
5 x 16
5 x 16
5 x 16
5 x 16
1
1
1
1
1
Handling & Storage
Cell Imaging
Double sided Microtube Rack
For handling of either 60 microtubes 0.2 ml or 80 microtubes 1.5 ml
>
>
>
>
Cat. No.
PAA010302B
PAA010302G
PAA010302R
PAA010302Y
60 PAA – The Cell Culture Company | www.paa.com
Two usable sides
For 60 microtubes 0.2 ml or 80 microtubes 1.5 ml
Autoclavable
4 different colors
Color
Material
Array (holes)
Purchase Unit (pcs)
Blue
Green
Red
Yellow
PP
PP
PP
PP
5 x 16 or 4 x 15
5 x 16 or 4 x 15
5 x 16 or 4 x 15
5 x 16 or 4 x 15
1
1
1
1
Handling & Storage
preparation and reactions of small volumes. With its lid and the
stack­able design it is also useful for storage of samples at room
temperature or in the refrigerator. Made of polypropylene our
PCR tube rack is fully autoclavable.
For 0.2 ml microtubes
Suitable for PCR strips and plates
With lid
Stackable
Autoclavable
5 different colors
Color
Material
Array (holes)
Purchase Unit (pcs)
PAA80096W
PAA80096R
PAA80096B
PAA80096G
PAA80096V
White
Red
Blue
Green
Violet
PP
PP
PP
PP
PP
8 x 12
8 x 12
8 x 12
8 x 12
8 x 12
1
1
1
1
1
Handling & Storage
Cat. No.
Cell Imaging
>
>
>
>
>
>
Immunoplates
Molecular Biology
The polymerase chain reaction (PCR) and its variants are standard techniques widely used in the field of molecular biology.
Usually PCR reactions are prepared in 0.2 ml microtubes and
carried out in a universal PCR cycler with adequate positions.
The PCR tube rack of PAA is an outstanding tool for handling,
Cell Culture
PCR Tube Racks
PAA – The Cell Culture Company | www.paa.com 61
Handling & Storage
Various experiments in the laboratory require serial pipetting
steps. For more convenience PAA offers disposable pipetting
reservoirs made of white polystyrene. The reservoirs are de-
signed for the use with single or multichannel pipettes. In order
to avoid contaminations they are supplied sterile and individually
packaged.
Immunoplates
Molecular Biology
Cell Culture
Reservoirs
Cat. No.
PAA22050X
PAA21002X
PAA21008X
PAA21012X
Suitable for single and multi channel pipettes
Notched volume graduation (PAA22050X)
Individually packed
Sterilized
Wells
Total Volume (ml)
Material
Sterilized
Pieces / Bag
Purchase Unit (pcs)
1
2
8
12
50
25
7
4
PS
PS
PS
PS
+
+
+
+
1
1
1
1
50
50
100
100
Handling & Storage
Cell Imaging
>
>
>
>
62 PAA – The Cell Culture Company | www.paa.com
Handling & Storage
The bottles are extremely lightweight, shatter-resistant and provide excellent gas barrier properties. The bottles are delivered in
brickpacks. Two or four brickpacks are double bagged and then
packed in master boxes.
Immunoplates
Molecular Biology
PAA offers a sterile square PET bottle in three different sizes for
various applications. The bottles meet current biological testing
standards. They are suitable for preserving and transporting
sensitive liquids for biopharmaceutical applications.
Cell Culture
Square Bottles
Cat. No.
PAA56125X
PAA56500X
PAA56000X
Available in sizes of 125, 500 and 1000 ml
Chemically and biologically inert
Shatter-resistant and lightweight
Gas impermeable
Clearly transparent
Sterilized
Cell Imaging
>
>
>
>
>
>
Volume (ml)
Material
Sterilized
Pieces / Brickpack
Brickpacks / Box
Purchase Unit (pcs)
125
500
1000
PET
PET
PET
+
+
+
24
24
12
4
2
2
96
48
24
PAA – The Cell Culture Company | www.paa.com 63
Handling & Storage
Brickpack
Handling & Storage
Immunoplates
able up to 134 °C and available as standard as well as highly
transparent bags.
In addition to the autoclave bags PAA offers a convenient
holder, which can be used on the bench and eases the handling
with contaminated biohazard material. This plastic coated
holder has three non-slip rubber feet and is suitable for bag size
200 x 300 mm.
Working with biohazardous materials requires correct
handling. In addition, the contaminated laboratory disposals like
cell culture flasks or pipette tips have to be destroyed in an
appropriate way before final removal.
PAA offers autoclave bags made of particular strong polypropylene film to use during daily laboratory routine. For autoclaving the bags should not be closed completely. They are autoclav-
> PP bags made of particularly strong film
(film thickness 50 µm) with a wide bottom seam
> For disposal of contaminated laboratory items
> Autoclavable up to +134 °C
> For autoclaving, the bag should be closed slightly
> Two different types available
Molecular Biology
Cell Culture
Autoclave Bags
Width x Length (mm)
Volume (l)
Max. Autoclave
Temperature
Pieces / Bag
PAA010711
200 x 300
2.5
134 °C
100
PAA010712
600 x 800
60.0
134 °C
500
PAA010721
200 x 300
2.5
121 °C
100
PAA010722
600 x 800
60.0
121 °C
500
Cat. No.
Purchase Unit (pcs)
100
(spender box)
500
100
(spender box)
500
Autoclave Bag Holder
Suitable for PAA010711 and PAA010721
PAA010700
Handling & Storage
Cell Imaging
Cat. No.
64 PAA – The Cell Culture Company | www.paa.com
Purchase Unit (pcs)
1
PAA – The Cell Culture Company | www.paa.com 65
66 PAA – The Cell Culture Company | www.paa.com
Appendix
Alphabetical Index . . . . . . . . . . . . . . . . . . . . . . . . . . . 68
Material Properties . . . . . . . . . . . . . . . . . . . . . . . . . . 70
Chemical Resistance of Various Materials . . . . . . . . . . 71
PAA – The Cell Culture Company | www.paa.com 67
Appendix
Alphabetical Index
Product Name
Page
2-Position Tubes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24
96 Well Immunoplates (ELISA Plate) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 36
96 Well Microplates for Colorimetric Assays . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12
384 Well Immunoplates . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37
A
Autoclave Bag Holder . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 64
B
Black & White Immunoplates . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37
Autoclave Bags . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 64
Blood Separation Tubes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22
C-Chip Neubauer Improved / Malassez . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14
Cell Culture Dishes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
Cell Culture Flasks . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8
Cell Culture Plates with Inserts . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13
Cell Culture Slides . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 46
Cell Lifter . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10
Cell Scrapers . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10
C
Center Well Culture Dish . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16
Confocal Dishes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16
Conical Centrifuge Tubes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23
Conical Centrifuge Tube Racks . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 59
Coverslips . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17
Cryo Boxes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 56
Cryotubes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 54
CytoCapture . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 48
D
E
F
Dispenser . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 28
Dispenser Tips . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 28
ELISA Plates (96 Well Immunoplates) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 36
Erlenmeyer Flasks . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32
Filter Pipette Tips . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27
68 PAA – The Cell Culture Company | www.paa.com
Appendix
Alphabetical Index
Product Name
Page
Imaging Chamber Accessories . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 45
Imaging Chambers . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 44
Imaging Chambers CG . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 44
I
Imaging Dishes CG (Cover Glass) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 43
Imaging Dishe Stage Frames . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 43
Imaging Plates CG, with Glass Bottom . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 41
Imaging Plates FC, with Fluorocarbon Film Bottom . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 42
Inserts for Cryotubes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 55
L
Loops & Needles . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33
Microtube Racks . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 60
Microtubes, 1.5 ml . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25
M
Microtubes, 2.0 ml . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25
Multiwell Plates . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30
Multiwell Tissue Culture Plates . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11
PCR Tubes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25
P
PCR Tube Racks . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 61
Petri Dishes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21
Pipette Tips . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26
R
Reservoirs . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 62
Serological Pipettes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20
Slide Tray Plates . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 47
Spreader . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33
S
Square Bottles . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 63
Square Dishes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15, 31
Storage Boxes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 57, 58
Strip Immunoplates . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 36
T
W
Test Tubes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24
Tray Plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15, 31
Working Rack for Cryotubes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 55
PAA – The Cell Culture Company | www.paa.com 69
Appendix
Material Properties
Letter symbol
Heat
resistance
Low-temperature
resist. (tested at)
Polypropylene
Polystyrene
Polyethylene
PP
PS
HDPE (High Density PE)
Permanent loading:
100 – 110 °C (212 – 230 °F)
Short-term max:
120 – 140 °C (248 – 284 °F)
Suitable for use in autoclaves:
121 °C (250 °F)
Permanent loading:
60 – 70 °C (140 – 158 °F)
Short-term max:
75 – 80 °C (167 – 176 °F)
Unsuitable for use in autoclaves
Permanent loading:
70 – 80 °C (158 – 176 °F)
Short-term max:
80 – 100 °C (176 – 212 °F)
Unsuitable for use in autoclaves
-192 °C (-314 °F)
-30 °C (-22 °F)
-40 °C (-40 °F)
Flammability
combustible
combustible
combustible
Density
0.90 g / cm
1.05 g / cm
0.95 g / cm3
Moisture
absorption
< 0.1 %
< 0.1 %
< 0.1 %
Optical
properties
translucent
shiny surface
transparent, shiny surface
with 90 %
transparency index
(at 400 – 800 nm)
translucent to
opaque
3
3
General
mechanical
properties
High fracture strength;
dimensionally stable; high
stiffness, strong and hard
Low fracture ductility; low warm
strength; excellent electrical insulation properties; not suitable for
use at high centrifugal loads
Rather low tensile strength
and surface hardness; high
ductility at soft and harder
levels; low resistance to stress
cracking; water repellent;
susceptible to electrostatic
charging
General
chemical
properties
> High resistance to aqueous solutions of inorganic salts, acids,
and alkalis, as well as to organic
solvents up to 60 °C
> Stable in alcohols, esters and
ketones
> Aromatic and halogenated
hydrocarbons, oxidizing substances such as concentrated
nitric acid and, at higher temperatures, fats, oils and waxes
all lead to swelling of PP
>H
igh resistance to salt and
caustic solutions, non-oxidizing
acids, alkalis and alcohols.
> P etroleum spirit, etheric oils,
strongly oxidizing reagents and
aromatics attack PS and give
rise to stress cracking
> Exhibits high resistance;
­diluted acids, alkalis,
­alcohol, oil and salt
solutions do not attack PE
> Concentrated oxidizing
acids such as nitric acid,
and halogens lead to
­disintegration
Disposal
PE / PP / PS are pure hydrocarbon compounds and therefore neutral to the environment.
By regulated incineration no harmful substances are produced.
Standards
The HDPE / PP / PE material used fulfills the requirements of Pharmacopoeia (USP)
Class 6 and Class 1 medical devices according to Directive 93/42.
70 PAA – The Cell Culture Company | www.paa.com
Appendix
Chemical Resistance of Various Materials
The Chemical Resistance Chart below is a general guide only. Many factors can affect the chemical resistance of a given product,
therefore you should test under your own conditions and always follow correct laboratory safety procedures.
Caution: Do not store strong oxidizing agents in plastic labware!
Resistance test conditions at +20 °C; the second to those at +50 °C.
Chemicals
High Density PE
HDPE
Polypropylene
PP
Polycarbonate
PC
Polystyrene
PS
1,2 Dichloroethane
1,2,4-Trichlorobenzene
1,4-Dioxane
2,2,4-Trimethylpentane
2,4 Dichlorophenol
2-Butanol
2-Methoxyethanol
2-Propanol
Acetaldehyde
Acetamide, Sat.
Acetic Acid, 5%
Acetic Acid, 50%
Acetic Acid, Glacial
Acetic Anhydride
Acetone
Acetonitrile
Acetophenone
Acrylonitrile
Adipic Acid
Allyl Alcohol
Aluminum Hydroxide
Amino Acids
Ammonia
Ammonia, 25%
Ammonium Glycolate
Ammonium Hydroxide, 30%
Ammonium Hydroxide, 5%
Ammonium Oxalate
Ammonium Salts
Amyl Alcohol
Amyl Chloride
Aniline
Aqua Regia
Arsenic Acid
Benzaldehyde
Benzenamine
Benzene
Benzoic Acid, Sat.
Benzyl Acetate
Benzyl Alcohol
Boric Acid
Bromine
Bromobenzene
Bromoform
Butadiene
Butyl Acetate
Butyl Chloride
Butyric Acid
No damage after 30 days of constant exposure
Little or no damage after 30 days of constant exposure
Some effect after 7 days of constant exposure
Immediate damage may occur; not recommended for continuous use
- Not measured
PAA – The Cell Culture Company | www.paa.com 71
Appendix
Chemicals
High Density PE
HDPE
Calcium Hydroxide
Calcium Hypochlorite
Carbazole
Carbon Disulphide
Carbon Tetrachloride
Cellosolve Acetate
Chlorine Water
Chlorine, 10% (Moist)
Chlorine, 10% in air
Chlorine, wet gas
Chloroacetic Acid
Chlorobenzene
Chloroform
Chromic Acid, 10%
Chromic Acid, 20%
Chromic Acid, 50%
Chromic:Surfuric Acid Mixture, 96%
Citric Acid, 10%
Cresol
Cyclohexane
Cyclohexanone
Cyclopentane
Decahydronaphtalene
Diacetone
Diacetone Alcohol
Dibutylphthalate
-
Diethyl Benzene
Diethyl Ether
Diethyl Ketone
Diethyl Malonate
Diethylamine
Diethylene Dioxide
Diethylene Glycol
Diethylene Glycol Ethyl Ether
Dimethyl Acetamide
Dimethyl Formamide
Dimethylsulphoxide (DMSO)
Dioxane
Dipropylene Glycol
Ethanol, 40%
Ether
Ethyl Acetate
Ethyl Alcohol (Absolute)
Ethyl Alcohol, 40%
Ethyl Alcohol, 96%
Ethyl Benzene
Ethyl Benzoate
Ethyl Butyrate
Ethyl Chloride
Ethyl Chloride, Liquid
Ethyl Cyanoacetate
Ethyl Lactate
Ethylene Chloride
Ethylene Glycol
Ethylene Glycol Monomethyl Ether
Ethylene Oxide Gas
Ethylene Oxide, 100%
No damage after 30 days of constant exposure
Little or no damage after 30 days of constant exposure
Some effect after 7 days of constant exposure
Immediate damage may occur; not recommended for continuous use
- Not measured
72 PAA – The Cell Culture Company | www.paa.com
Polypropylene
PP
Polycarbonate
PC
Polystyrene
PS
Appendix
Chemicals
High Density PE
HDPE
Polypropylene
PP
Polycarbonate
PC
Polystyrene
PS
Fatty Acids
Fluorine
Formaldehyde, 10%
Formaldehyde, 40%
Formalin, 10%
Formalin, 40%
Formic Acid
Formic Acid, 100%
Formic Acid, 3%
Formic Acid, 50%
Formic Acid, 85%
Freon TF
Glutaraldehyde
Glycerine (Glycerol)
Hexane
Hydrazine
Hydrobromic Acid, 4%
Hydrobromic Acid, 48%
Hydrobromic Acid, 69%
-
Hydrochloric Acid, 20%
Hydrochloric Acid, 35%
Hydrochloric Acid, 5%
Hydrogen Peroxide, 3%
Hydrogen Peroxide, 30%
Hydrogen Peroxide, 90%
Isobutanol
Isopropanol, 100%
Isopropyl Acetate
Isopropyl Benzene
Isopropyl Ether
Lactic Acid, 3%
Lactic Acid, 85%
Lodine Crystals
Mercury
Methanol, 100%
Methoxyethyl Oleate
Methyl Acetate
Methyl Ethyl Ketone
Methyl Isobutyl Ketone (MIBK)
Methyl Propyl Ketone
Methylene Chloride
Methyl-t-Butyl Ether
n-Amyl Acetate
n-Butanol
n-Butyl Acetate
n-Decane
n-Heptane
Nitric Acid, 10%
Nitric Acid, 20%
Nitric Acid, 50%
Nitric Acid, 70%
Nitrobenzene
Nitromethane
n-Octane
o-Dichlorobenzene
Oil, Mineral
Oxalic Acid, 10%
Ozone
No damage after 30 days of constant exposure
Little or no damage after 30 days of constant exposure
Some effect after 7 days of constant exposure
Immediate damage may occur; not recommended for continuous use
- Not measured
PAA – The Cell Culture Company | www.paa.com 73
Appendix
Chemicals
High Density PE
HDPE
p-Chloroacetophenone
p-Dichlorobenzene
Perchloric Acid
Perchloric Acid, 70%
Perchloroethylene
Phenol, 100%
Phenol, 50%
Phenol, Crystals
Phenol, Liquid
Phosphoric Acid, 5%
Phosphoric Acid, 85%
Picric Acid
Potassium Hydroxide, 1%
Potassium Hydroxide, 30%
Potassium Hydroxide, Concentrated
Potassium Permanganate
Propane Gas
Proprionic Acid
Propylene Glycol
Propylene Oxide
Pyridine
Resorcinol, 5%
Resorcinol, Sat.
Salicylaldehyde
Salicylic Acid, Sat.
Salt Solutions, Metallic
Silicone Oil
Silver Nitrate
Sodium Dichromate
Sodium Hydroxide, 50%
Sodium Hydroxide, 1%
Sodium Hydroxide, 10%
Sodium Hypochlorite, 15%
Stearic Acid
Sulfur dioxide
Sulfur Dioxide, wet or dry
Sulfur Salts
Sulfuric Acid, 96%
Sulfuric Acid, 20%
Sulfuric Acid, 30%
Sulfuric Acid, 6%
Sulfuric Acid, 60%
Sulfuric Acid, 98%
Tartaric Acid
Tetrahydrofuran
Thionyl Chloride
Tincture of Iodine
Toluene
Tributyl Citrate
Trichloroacetic Acid (TCA)
Trichloroethane
Trichloroethylene
Triethylene Glycol
Tripropylene Glycol
Tris Buffer, Solution
Urea
Xylene
No damage after 30 days of constant exposure
Little or no damage after 30 days of constant exposure
Some effect after 7 days of constant exposure
Immediate damage may occur; not recommended for continuous use
- Not measured
74 PAA – The Cell Culture Company | www.paa.com
Polypropylene
PP
Polycarbonate
PC
Polystyrene
PS
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