Int. J. Mol. Sci. 2016, 17, 951; doi:10.3390/ijms17060951
S1 of S10
Supplementary Materials: Duplication and
Remolding of tRNA Genes in the Mitochondrial
Genome of Reduvius tenebrosus
(Hemiptera: Reduviidae)
Pei Jiang, Hu Li, Fan Song, Yao Cai, Jianyun Wang, Jinpeng Liu and Wanzhi Cai
Figure S1. Sequence matching between the non-coding regions (NCR) parts and ND2 and control
region (CR). The identical bases between two sequences are in navy blue and the white and sky blue
ones indicate different bases between them.
Table S1. Organization of Reduvius tenebrosus mitochondrial (mt) genome. “F” indicates genes
encoded on the majority strand and “R” indicates genes annotated on the minority strand. “T-” stands
for the incomplete stop codon (a single T).
Gene
Direction
trnI1
trnM
ND2
trnW
trnC
trnY
COI
F
F
F
F
R
R
F
Location
1
107
176
1176
1233
1303
1372
64
175
1177
1240
1294
1368
2905
Size
Anticodon
64
69
1002
65
62
66
1534
GAT
CAT
Codon
Start
Codon
Stop
ATC
TAA
ATG
T-
TCA
GCA
GTA
Intergenic
Nucleotides
0
42
0
−2
−8
8
3
Int. J. Mol. Sci. 2016, 17, 951; doi:10.3390/ijms17060951
S2 of S10
Table S1. Cont.
Gene
Direction
trnL2
COII
trnK
trnD
ATP8
ATP6
COIII
trnG
ND3
trnA
trnR
trnN
trnS1
trnE
trnF
ND5
trnH
ND4
ND4L
trnT
trnP
ND6
CytB
trnS2
ND1
trnL1
lrRNA
trnV
srRNA
control region
trnQ
trnI2
NCR
F
F
F
F
F
F
F
F
F
F
F
F
F
F
R
R
R
R
R
F
R
F
F
F
R
R
R
R
R
F
R
F
F
Location
2906
2971
3672
3742
3805
3957
4627
5414
5476
5829
5891
6058
6124
6194
6257
6324
8035
8097
9417
9716
9777
9845
10,345
11,480
11,696
12,614
12,679
13,934
14,002
14,786
16,037
16,105
16,174
2970
3654
3741
3804
3963
4640
5413
5475
5829
5888
5954
6124
6192
6258
6323
8034
8096
9423
9713
9776
9842
10,345
11,481
11,550
12,613
12,678
13,933
14,001
14,785
16,036
16,105
16,173
17,090
Size
Anticodon
65
684
70
63
159
684
787
62
354
60
64
67
69
65
67
1711
62
1327
297
61
66
501
1137
71
918
65
1255
68
784
1251
69
69
917
TAA
Codon
Start
Codon
Stop
ATT
TAA
ATT
ATG
ATG
TAA
TAG
T-
ATA
TAG
ATT
T-
ATG
GTG
TTAA
ATT
ATG
TAA
TAA
GTG
TAG
CTT
GTC
TCC
TGC
TCG
GTT
GCT
TTC
GAA
GTG
TGT
TGG
TGA
TAG
TAC
TTG
GAT
Intergenic
Nucleotides
0
0
17
0
0
−7
−14
0
0
−1
2
103
−1
1
−2
0
0
0
−7
2
0
2
−1
−2
145
0
0
0
0
0
0
−1
0
Table S2. Base composition in R. tenebrosus mt genome.
Feature
Whole genome
Protein-coding genes
First codon position
Second codon position
Third codon position
Protein-coding genes-J
First codon postion
Second codon position
Third codon position
Protein-coding genes-N
First codon position
Second codon postion
Third codon position
Control region
Total
17,090
11,064
3688
3688
3688
6819
2273
2273
2273
4245
1415
1415
1415
1251
%T
27.5
38.2
31.9
45.9
36.7
30.6
24.3
43.6
23.9
50.3
44.1
49.5
57.3
28.9
%C
20.4
17.8
15.9
19.3
18.1
22
19.4
21.4
25
11.1
10.2
15.9
7.1
21.7
%A
39.7
27.6
31.5
18.8
32.5
32.8
35.5
20.1
42.8
19.2
25.1
16.7
15.9
37.2
%G
12.5
16.5
20.7
16.1
12.6
14.6
20.7
15
8.2
19.4
20.6
17.9
19.7
12.2
%A+T
67.2
65.8
63.4
64.7
69.2
63.4
59.8
63.7
66.7
69.5
69.2
66.2
73.2
66.1
%G+C
32.8
34.2
36.6
35.3
30.8
36.6
40.2
36.3
33.3
30.5
30.8
33.8
26.8
33.9
AT-Skew
0.18
−0.16
−0.01
−0.42
−0.06
0.03
0.19
−0.37
0.28
−0.45
−0.27
−0.50
−0.57
0.13
GC-Skew
−0.24
−0.04
0.13
−0.09
−0.18
−0.20
0.03
−0.18
−0.51
0.27
0.34
0.06
0.47
-0.28
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S3 of S10
Table S3. Structural features and codon usage for isoleucine (Ile) of sequenced assassin bug mt genomes.
Subfamily
Species
Rearragement
Length (bp)
AT %
Triatominae
Salyavatinae
Harpactorinae
Ectrichodiinae
Peiratinae
Peiratinae
Peiratinae
Peiratinae
Peiratinae
Peiratinae
Stenopodinae
Tribelocephalinae
Reduviinae
Triatoma dimidiata
Valentia hoffmanni
Agriosphodrus dohrni
Brontostoma colossus
Sirthenea flavipes
Peirates arcuatus
Peirates fulvescens
Peirates atromaculatus
Peirates turpis
Peirates lepturoides
Oncocephalus breviscutum
Opistoplatys sp.
Reduvius tenebrosus
none
none
none
A-R-N→R-A-R-N
none
none
none
none
none
none
none
none
I-Q-M→Q-I-I-M
17,019
15,625
16,470
16,625
15,961
16,176
15,702
16,151
15,703
15,932
15,948
15,615
17,090
69.5%
73.7%
72.2%
73.4%
71.8%
71.3%
71.9%
71.0%
72.0%
72.4%
74.4%
75.6%
67.2%
ATT
281 *
325
313
328
328
343 *
318
309
312
329
367 *
383 *
251 *
Codons for Ile
ATC ATT + ATC
81 *
362
41
366
48
361
56
384
51
379
42 *
385
53
371
61
370
58
370
59
388
24 *
391
38 *
421
114 *
365
GenBank Accession
NC_002609
NC_012823
NC_015842
NC_024745
NC_020143
NC_024264
KF913537
KF913539
KF913540
KF913541
NC_022816
KC887533
present study
Values with an asterisk signify that relative frequency of codons for ATT vs. ATC is significantly (p < 0.05) different from the averaged among 13 assassin bugs.
Int. J. Mol. Sci. 2016, 17, 951; doi:10.3390/ijms17060951
S4 of S10
Table S4. Codon usage of protein-coding genes in the R. tenebrosus mt genome.
Amino Acid
Codon
N
RSCU
N+
RSCU
N−
RSCU
Phe (F)
UUU
UUC
UUA
UUG
CUU
CUC
CUA
CUG
AUU
AUC
AUA
AUG
GUU
GUC
GUA
GUG
UCU
UCC
UCA
UCG
CCU
CCC
CCA
CCG
ACU
ACC
ACA
ACG
GCU
GCC
GCA
GCG
UAU
UAC
235
98
186
103
76
41
104
27
251
114
184
66
89
24
66
28
106
48
71
7
60
39
36
5
69
24
74
10
49
38
65
10
108
56
1.41
0.59
2.08
1.15
0.85
0.46
1.16
0.30
1.38
0.62
1.47
0.53
1.72
0.46
1.28
0.54
2.25
1.02
1.51
0.15
1.71
1.11
1.03
0.14
1.56
0.54
1.67
0.23
1.21
0.94
1.60
0.25
1.32
0.68
78
83
87
12
32
39
94
22
155
101
147
22
30
17
57
15
23
39
63
2
31
36
35
5
35
23
73
8
16
31
62
4
31
46
0.97
1.03
1.83
0.25
0.67
0.82
1.97
0.46
1.21
0.79
1.74
0.26
1.01
0.57
1.92
0.50
0.88
1.50
2.42
0.08
1.16
1.35
1.31
0.19
1.01
0.66
2.10
0.23
0.57
1.10
2.19
0.14
0.81
1.19
157
15
99
91
44
2
10
5
96
13
37
44
59
7
9
13
83
9
8
5
29
3
1
0
34
1
1
2
33
7
3
6
77
10
1.83
0.17
2.37
2.18
1.05
0.05
0.24
0.12
1.76
0.24
0.91
1.09
2.68
0.32
0.41
0.59
3.88
0.42
0.37
0.23
3.52
0.36
0.12
0.00
3.58
0.11
0.11
0.21
2.69
0.57
0.24
0.49
1.77
0.23
Leu (L)
Ile (I)
Met (M)
Val(V)
Ser (S)
Pro (P)
Thr (T)
Ala (A)
Tyr (Y)
Int. J. Mol. Sci. 2016, 17, 951; doi:10.3390/ijms17060951
S5 of S10
Table S4. Cont.
Amino Acid
Codon
N
RSCU
N+
RSCU
N−
RSCU
Stop (*)
UAA
UAG
CAU
CAC
CAA
CAG
AAU
AAC
AAA
AAG
GAU
GAC
GAA
GAG
UGU
UGC
UGA
UGG
CGU
CGC
CGA
CGG
AGU
AGC
AGA
AGG
GGU
GGC
GGA
GGG
6
3
37
38
44
25
71
61
54
37
54
24
53
30
38
18
74
29
17
4
26
8
36
15
82
14
59
27
79
67
0.99
1.01
1.28
0.72
1.08
0.92
1.19
0.81
1.38
0.62
1.28
0.72
1.36
0.64
1.44
0.56
0.68
0.16
1.03
0.32
0.76
0.32
3.26
0.56
1.02
0.47
1.36
1.16
26
35
41
11
35
58
51
18
26
21
47
10
5
13
58
13
3
3
24
5
9
9
61
2
9
15
73
38
0.85
1.15
1.58
0.42
0.75
1.25
1.48
0.52
1.11
0.89
1.65
0.35
0.56
1.44
1.63
0.37
0.34
0.34
2.74
0.57
0.35
0.35
2.35
0.08
0.27
0.44
2.16
1.13
11
3
3
14
36
3
3
19
28
3
6
20
33
5
16
16
14
1
2
3
27
6
21
12
50
12
6
29
1.57
0.43
0.35
1.65
1.85
0.15
0.27
1.73
1.81
0.19
0.46
1.54
1.74
0.26
1.00
1.00
2.80
0.20
0.40
0.60
1.26
0.28
0.98
0.56
2.06
0.49
0.25
1.20
His (H)
Gln (Q)
Asn (N)
Lys (K)
Asp (D)
Glu (E)
Cys (C)
Trp (W)
Arg (R)
Ser (S)
Gly (G)
N, the number of codons used in protein-coding genes; RSCU, relative synonymous codon usage.
Values in bold type stand for the most commonly used codon for amino acid. Underlined stand for
the cognate codon of tRNA for each amino acid.
Int. J. Mol. Sci. 2016, 17, 951; doi:10.3390/ijms17060951
S6 of S10
Table S5. Codon usage of protein-coding genes in 13 sequenced assassin bug mt genomes.
Species
Triatoma dimidiata
Valentia hoffmanni
Agriosphodrus dohrni
Brontostoma colossus
Sirthenea flavipes
Peirates arcuatus
Peirates fulvescens
Peirates atromaculatus
Peirates turpis
Peirates lepturoides
Oncocephalus breviscutum
Opistoplatys sp.
Reduvius tenebrosus
Average
Species
T. dimidiata
V. hoffmanni
A. dohrni
B. colossus
S. flavipes
P. arcuatus
P.fulvescens
P. atromaculatus
P. turpis
P. lepturoides
O. breviscutum
Opistoplatys sp.
R. tenebrosus
Average
UCU
130
108
110
108
129
117
121
123
124
123
125
105
106
118
UUU
250
292
282
276
283
278
269
276
273
289
287
313
235
277
UCC
26
28
22
22
18
27
18
18
16
12
20
23
48
23
Phe
UUC
85
55
71
82
62
64
75
69
71
54
61
43
98
68
Ser
UCA
76
101
88
85
77
77
83
84
85
94
86
80
71
84
%
0.25
0.16
0.20
0.23
0.18
0.19
0.22
0.20
0.21
0.16
0.18
0.12
0.29
0.20
UCG
3
2
2
9
6
8
8
5
6
2
6
4
7
5
UUA
219
323
298
295
264
275
331
330
332
315
325
367
186
297
%
0.12
0.13
0.11
0.14
0.10
0.15
0.11
0.10
0.10
0.06
0.11
0.13
0.24
0.12
UUG
95
52
51
74
68
56
61
60
60
49
45
38
103
62
CCU
67
69
54
63
53
52
63
63
62
74
70
71
60
63
CUU
89
82
78
54
67
76
56
60
60
75
84
57
76
70
CCC
21
19
22
20
25
33
25
26
27
17
8
16
39
23
Leu
CUC
31
9
22
10
17
15
22
17
17
12
9
10
41
18
Pro
CCA
41
45
47
51
53
50
50
53
52
50
56
41
36
48
CUA
81
53
55
68
82
68
46
50
46
54
35
33
104
60
CCG
4
0
2
5
8
4
3
3
3
3
2
7
5
4
%
0.19
0.14
0.19
0.18
0.24
0.27
0.20
0.20
0.21
0.14
0.07
0.17
0.31
0.19
CUG
10
7
17
10
11
18
10
9
12
9
6
2
27
11
ACU
77
76
97
61
76
79
65
66
66
77
71
77
69
74
%
0.26
0.13
0.17
0.18
0.19
0.18
0.18
0.16
0.17
0.14
0.12
0.10
0.32
0.18
AUU
281
325
313
328
328
343
318
309
312
329
367
383
251
322
ACC
37
16
25
23
15
21
22
20
20
16
17
22
24
21
Ile
AUC
81
41
48
56
51
42
53
61
58
59
24
38
114
56
Thr
ACA
69
92
80
101
89
87
93
96
95
84
91
58
74
85
%
0.22
0.11
0.13
0.15
0.13
0.11
0.14
0.16
0.16
0.15
0.06
0.09
0.31
0.15
ACG
5
2
5
7
6
5
3
1
2
1
5
5
10
4
AUA
193
250
222
247
217
209
216
214
214
221
251
255
184
223
%
0.22
0.10
0.14
0.16
0.11
0.14
0.14
0.11
0.12
0.10
0.12
0.17
0.19
0.14
Met
AUG
58
31
39
43
64
50
49
51
58
47
22
29
66
47
GCU
60
65
56
48
66
46
42
41
42
55
66
53
49
53
%
0.23
0.11
0.15
0.15
0.23
0.19
0.18
0.19
0.21
0.18
0.08
0.10
0.26
0.17
GCC
35
15
23
12
20
29
21
21
19
19
15
26
38
23
GUU
113
92
83
90
91
91
106
102
103
106
94
71
89
95
Ala
GCA
61
60
67
55
54
68
79
78
78
82
49
54
65
65
GUC
21
9
19
13
20
15
11
15
15
12
5
11
24
15
GCG
4
2
5
3
4
3
5
5
5
4
6
4
10
5
Val
GUA
76
86
82
58
78
78
74
75
74
71
74
68
66
74
%
0.24
0.12
0.19
0.13
0.17
0.22
0.18
0.18
0.17
0.14
0.15
0.22
0.30
0.19
UAU
130
138
137
145
136
139
138
140
140
140
161
171
108
140
GUG
19
9
19
7
16
15
17
17
17
7
13
11
28
15
Tyr
UAC
37
22
38
29
33
28
24
23
23
24
11
14
56
28
%
0.17
0.09
0.19
0.12
0.18
0.15
0.13
0.15
0.15
0.10
0.10
0.14
0.25
0.15
%
0.22
0.14
0.22
0.17
0.20
0.17
0.15
0.14
0.14
0.15
0.06
0.08
0.34
0.17
Int. J. Mol. Sci. 2016, 17, 951; doi:10.3390/ijms17060951
Species
T. dimidiata
V. hoffmanni
A. dohrni
B. colossus
S. flavipes
P. arcuatus
P.fulvescens
P. atromaculatus
P. turpis
P. lepturoides
O. breviscutum
Opistoplatys sp.
R. tenebrosus
Average
CAU
49
58
55
55
46
59
49
47
48
51
57
57
37
51
Species
T. dimidiata
V. hoffmanni
A. dohrni
B. colossus
S. flavipes
P. arcuatus
P.fulvescens
P. atromaculatus
P. turpis
P. lepturoides
O. breviscutum
Opistoplatys sp.
R. tenebrosus
Average
His
CAC
30
21
24
22
31
17
30
30
29
27
17
13
38
25
UGA
77
90
83
78
88
85
89
86
86
90
91
81
74
84
%
0.38
0.27
0.30
0.29
0.40
0.22
0.38
0.39
0.38
0.35
0.23
0.19
0.51
0.33
CAA
48
57
47
47
47
47
44
47
47
47
55
56
44
49
Trp
UGG
23
11
22
16
14
18
16
17
17
13
12
17
29
17
%
0.23
0.11
0.21
0.17
0.14
0.17
0.15
0.17
0.17
0.13
0.12
0.17
0.28
0.17
S7 of S10
Gln
CAG
9
5
16
9
10
11
10
8
8
8
9
8
25
10
CGU
18
19
16
14
20
19
16
16
16
15
19
25
17
18
%
0.16
0.08
0.25
0.16
0.18
0.19
0.19
0.15
0.15
0.15
0.14
0.13
0.36
0.18
AAU
105
130
130
128
115
123
117
117
117
125
144
169
71
122
CGC
1
1
2
4
1
5
2
2
2
2
1
2
4
2
Asn
AAC
44
29
40
38
42
28
34
34
34
26
17
20
61
34
Arg
CGA
33
29
32
25
30
28
32
31
31
32
30
21
26
29
%
0.30
0.18
0.24
0.23
0.27
0.19
0.23
0.23
0.23
0.17
0.11
0.11
0.46
0.22
CGG
4
4
2
6
3
2
4
5
5
5
5
5
8
4
AAA
50
69
69
92
68
65
75
71
71
59
84
81
54
70
%
0.09
0.09
0.08
0.20
0.07
0.13
0.11
0.13
0.13
0.13
0.11
0.13
0.22
0.12
Lys
AAG
35
24
39
15
32
24
25
29
31
39
17
15
37
28
AGU
27
28
35
34
33
28
33
31
31
31
35
33
36
32
%
0.41
0.26
0.36
0.14
0.32
0.27
0.25
0.29
0.30
0.40
0.17
0.16
0.41
0.29
AGC
7
10
9
9
8
6
4
6
6
5
4
4
15
7
GAU
48
54
54
54
52
56
57
56
57
64
62
59
54
56
Ser
AGA
92
91
82
72
85
92
93
94
94
89
87
72
82
87
Asp
GAC
25
13
21
15
23
19
18
19
18
12
6
8
24
17
AGG
9
4
11
8
3
14
5
5
3
7
3
4
14
7
%
0.34
0.19
0.28
0.22
0.31
0.25
0.24
0.25
0.24
0.16
0.09
0.12
0.31
0.23
%
0.12
0.11
0.15
0.14
0.09
0.14
0.07
0.08
0.07
0.09
0.05
0.07
0.20
0.11
%: The ratios of codons ending in G or C vs. total codons of each amino acid.
GAA
61
63
70
58
68
75
72
72
72
71
81
71
53
68
GGU
72
67
68
69
74
66
81
80
78
79
88
63
59
73
Glu
GAG
26
20
21
12
20
16
16
19
17
17
12
15
30
19
GGC
20
8
14
19
7
9
12
12
13
10
0
13
27
13
%
0.30
0.24
0.23
0.17
0.23
0.18
0.18
0.21
0.19
0.19
0.13
0.17
0.36
0.21
Gly
GGA
109
115
76
111
106
115
96
97
98
102
120
110
79
103
UGU
41
47
53
56
34
49
52
51
51
46
46
46
38
47
GGG
27
33
45
31
34
39
34
34
33
34
19
34
67
36
Cys
UGC
14
10
1
6
18
12
8
9
9
12
6
6
18
10
%
0.21
0.18
0.29
0.22
0.19
0.21
0.21
0.21
0.21
0.20
0.08
0.21
0.41
0.22
%
0.25
0.18
0.02
0.10
0.35
0.20
0.13
0.15
0.15
0.21
0.12
0.12
0.32
0.17
Int. J. Mol. Sci. 2016, 17, 951; doi:10.3390/ijms17060951
S8 of S10
Table S6. Start and stop codons of protein-coding genes in sequenced assassin bug mt genomes.
Gene
ND2
COI
COII
ATP8
ATP6
COIII
ND3
ND5
ND4
ND4L
ND6
CytB
ND1
T. dimidiata
ATC/TAG
ATG/TATA/TATA/TAA
ATG/TAA
ATG/TAATA/TAGTG/TAATG/TAA
ATG/TAA
ATA/TAA
ATG/TATA/TAA
V. hoffmanni
ATG/TAA
ATG/TATA/TATC/TAA
ATG/TAG
ATG/TATT/TATT/TATG/TAA
ATT/TAA
ATA/TAA
ATG/TAG
GTG/TAA
A. dohrni
ATT/TAA
ATG/TATC/TAA
ATT/TAA
ATG/TAA
ATG/TATA/TAA
ATG/TATG/TAA
ATG/TAA
ATG/TAA
ATG/TAA
GTG/TAA
S. flavipes
ATT/TAA
ATG/TAA
ATA/TAA
ATA/TAA
ATG/TAG
ATG/TATA/TATT/TATG/TAA
GTG/TAA
ATT/TAA
ATG/TAG
GTG/T-
B. colossus
ATT/TATG/TATT/TATT/TAA
ATG/TAA
ATG/TAA
ATA/TAA
ATT/T
ATG/TAA
ATT/TAA
ATA/TAA
ATG/TATT/TAA
P. arcuatus
ATG/TAA
ATG/TATC/TATT/TAA
ATG/TAA
ATG/TATT/TAA
ATT/TAA
ATG/TAA
ATG/TAA
ATA/TAA
ATG/TAA
ATG/TAG
Start/Stop Codon
Opistoplatys sp.
O. breviscutum
ATT/TATT/TAA
ATG/TATG/TAA
ATG/TATA/TATA/TAA
ATA/TAA
ATG/TAG
ATG/TAA
ATG/TATG/TATT/TAATT/TAA
ATT/TATT/TATG/TAG
ATG/TAA
GTG/TAA
ATT/TAG
ATG/TAA
ATA/TAA
ATA/TATG/TAG
ATG/TAG
ATA/TAA
R. tenebrosus
ATC/TAA
ATG/TATT/TAA
ATT/TAA
ATG/TAG
ATG/TATA/TAG
ATT/TATG/TGTG/TAA
ATT/TAA
ATG/TAA
GTG/TAG
Unconventional start codons were highlighted by grey color.
P. fulvescens
ATG/TAA
ATG/TATC/TATA/TAA
ATG/TAG
ATG/TATT/TATT/TATG/TAA
ATG/TAA
ATT/TAA
ATG/TAA
ATA/TAA
P. atromaculatus
ATG/TAA
ATG/TATC/TATA/TAA
ATG/TAG
ATG/TATT/TATT/TATG/TAA
ATG/TAA
ATT/TAA
ATG/TAA
ATA/TAA
P. turpis
ATG/TAA
ATG/TATC/TATA/TAA
ATG/TAG
ATG/TATT/TATT/TATG/TAA
ATG/TAA
ATT/TAA
ATG/TAA
ATA/TAA
P. lepturoides
ATG/TAA
ATG/TATC/TATT/TAA
ATG/TAG
ATG/TATT/TATT/TATG/TAA
ATG/TAA
ATC/TAA
ATG/TAA
ATA/TAA
Int. J. Mol. Sci. 2016, 17, 951; doi:10.3390/ijms17060951
S9 of S10
Table S7. Primer pairs used in this study.
No. Fragment
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
21
Primer ID
TM-J210
TW-N1284
N2-J586
C1-N1738
F-1738
R-2130
C1-J2183
TL2-N3014
C1-J2756
C2-N3665
F-3665
R-4552
F-4552
R-4792
F-4792
R-6172
TN-J6172
N5-N7211
F-7211
R-7572
N5-J7572
N4-N8727
N4-J8641
N4L-J9629
F-9629
R-11335
CB-J11335
N1-N12067
N1-J11876
N1-N12595
N1-J12261
LR-N13000
LR-J12888
LR-N13889
LR-J13342
SR-N14220
SR-J14197
SR-N14745
F-14745
R-15114
F-15114
R-210
Nucleotide Sequence (5′–3′)
AATTAAGCTATTAGGTTCATACCC
TTAACTTTGAAGGTTAATAGTTT
CCATTCCATTTYTGATTTCC
TTTATTCGTGGAAATGCTATGTC
TTCAGCCACCCTATCCCTA
CGAGTGTCTACATCCATACCA
CAACATTTATTTTGATTTTTTGG
TCCAATGCACTAATCTGCCATATT
ACATTTTTTCCTCAACATTT
CCACAAATTTCTGAACACTG
TCTAACCCAAAGTCAGCCC
GGTCAAATGCTATGATGCC
TGAAAGGACTAAGTTACCG
TATGTGGTTGATGGAGGA
ACAAGCCCTCTTTATCAC
AGGTTATGGATTCTGGGT
AGAGGCAATTTATTGTTAATAA
TTAAGGCTTTATTATTTATATGTGC
GGAAGACCACACAAGGAAAT
TTATTGGGAGGGACTCTGC
AAACGGAAACTGAGCTCTCTTAGT
AAATCTTTAATTGCCTATTCTTC
CCAGAAGAACACAAACCATG
GTTTGTGAGGGTGCAATAGG
CGCACTCAAACGCTCAGGCT
CTACTGTGAGAAGTTGCCCTAC
CATATTCAACCAGAATGATA
AATCGTTCTCCATTTGATTTTGC
CGAGGTAAAGTACCACGTACTCA
GTTGGATTTCTAACTTTATTRGARCG
TACCTCATAAGAAATAGTTTGAGC
TTACCTTAGGGATAACAGCGTAA
CCGGTCTGAACTCAGATCATGTA
ATTTATTGTACCTTTTGTATCAG
CCTTTGCACAGTCAAAATACTGC
TTATGCACACATCGCCCGTC
GTAAAYCTACTTTGTTACGACTT
GTGCCAGCAAYCGCGGTTATAC
ATTAATGAGAGTGACGGGCGAT
TATGTGGTTGATGGAGGAGTAC
ATGTCCTACTATCGTCAC
GGGATGAATGCAATTAGG
Reference
[49]
[49]
[49]
[49]
Present study
Present study
[49]
[49]
[49]
[49]
Present study
Present study
Present study
Present study
Present study
Present study
[49]
[49]
Present study
Present study
[49]
[49]
[49]
[49]
Present study
Present study
[49]
[49]
[49]
[49]
[49]
[49]
[49]
[49]
[49]
[49]
[49]
[49]
Present study
Present study
Present study
Present study
Int. J. Mol. Sci. 2016, 17, 951; doi:10.3390/ijms17060951
S10 of S10
Table S8. Taxa used in this study.
Order
Infroorder
Superfamily
Family
Species
GenBank
Accession
Pentatomoidea
Cydnidae
Pentatomidae
Macroscytus subaeneus
Nezara viridula
NC_012457
NC_011755
Reduvioidea
Reduviidae
Miroidea
Miridae
Agriosphodrus dohrni
Oncocephalus breviscutum
Sirthenea flayipes
Triatoma dimidiata
Valentia hoffmanni
Brontostoma colossus
Peirates lepturoides
Peirates arcuatus
Reduvius tenebrosus
Adelphocoris fasciaticollis
Apolygus lucorum
Adelphocoris nigritylus
Lygus hesperus
Lygus lineolaris
Nesidiocoris tenuis
Corythucha ciliata
Pseudacysta perseae
Alloeorhynchus bakeri
Gorpis annulatus
Gorpis humeralis
Himacerus apterus
Nabis apicalis
Orius niger
NC_015842
NC_022816
HQ645959
NC_002609
NC_012823
NC_024745
NC_026672
NC_024264
Present study
NC_023796
NC_023083
NC_027144
NC_024641
NC_021975
NC_022677
NC_022922
KM278221
HM235722
JF907591
NC_019593
JF927831
JF907590
EU427341
Outgroup
Ingroup
Hemiptera
Cimicomorpha
Tingidae
Naboidea
Nabidae
Cimicoidea
Anthocoridae