NicoMitroCurriculumVitae
NICOMITRO,Ph.D.
CURRICULUMVITAE
PERSONALINFORMATION:
Nationality: Birthdate: WorkAddress:
Italian
May16,1976
DepartmentofPharmacologicalandBiomolecularSciences–DiSFeB,
“Giovanni Galli” Laboratory of Biochemistry of Metabolism and Mass
Spectrometry, Università degli Studi di Milano, Via Balzaretti 9, 20133,
Milan,Italy.
[email protected]
E-mail:
Researcheruniqueidentifier(s):ORCIDID0000-0002-5000-3619;ScopusAuthorID6506384818.
URLforwebsite:http://www.unimi.it/chiedove/ENG/schedaPersonaXML.jsp?matricola=15651
CURRENTPOSITION
2015–Todate
Associate Professor of Biochemistry, Department of Pharmacological and
BiomolecularSciences,UniversitàdegliStudidiMilano,Italy.
PREVIOUSPOSITIONS
2011–2015
Assistant Professor of Biochemistry, Department of Pharmacological and
BiomolecularSciences,UniversitàdegliStudidiMilano,Italy.
2008–2011
Principal Investigator of the Giovanni Armenise-Harvard Foundation Career
Development Award, Department of Pharmacological and Biomolecular
Sciences,UniversitàdegliStudidiMilano,Italy.
EDUCATIONANDRESEARCHTRAINING
2006–2008
Post-doctoral fellow, Department of Chemical Physiology and The Skaggs
Institute for Chemical Biology, The Scripps Research Institute, La Jolla, CA,
USA.Supervisor:Dr.EnriqueSaez
2005–2006
Post-doctoral fellow, Genomics Institute of the Novartis Research
Foundation,SanDiego,CA,USA.Supervisor:Dr.EnriqueSaez
2001–2006
Ph.D. in Experimental medicine: atherosclerosis, School of Medicine,
UniversitàdegliStudidiSiena,Italy.Supervisor:Prof.MaurizioCrestani.
1995–2001
Degree in Pharmaceutical Biotechnology, School of Pharmacy, Università
degliStudidiMilano,Italy.
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NicoMitroCurriculumVitae
PUBLICATIONS.
Bibliometricparameters(accordingtoWebofScience)
Totalpublicationsinpeer-reviewedjournal:57
Researcharticles:42;Reviews:15;Bookchapters:3
H-index:23
Citations(excludingself-citations):1725
Rankofpublicationsinquartile:Q1:70.2%;Q2:14.9%;Q3:6.4%;Q4:8.5%.
Averageimpactfactoroffirst/correspondingpublications(excludingreviews):7.6
Selectedpublicationsinchronologicalorder
1. Mauro C, Smith J, Cucchi D, Coe D, Fu H, Bonacina F, Baragetti A, Cermenati G, Caruso D,
Mitro N, Catapano AL, Ammirati E, Longhi MP, Okkenhaug K, Norata GD, Marelli-Berg FM.
“Obesity-Induced Metabolic Stress Leads to Biased Effector Memory CD4+ T Cell
DifferentiationviaPI3Kp110δ-Akt-MediatedSignals”.CellMetabolism.2017Mar7;25(3):593609.
2. CermenatiG,GiattiS,AudanoM,PesaresiM,SpezzanoR,CarusoD,MitroN*andMelcangi
RC*. “Diabetes alters myelin lipid profile in rat cerebral cortex: Protective effects of
dihydroprogesterone”. Journal of Steroid Biochememistry and Molecular Biology. 2017
Apr;168:60-70.*Bothcorrespondingauthors.
3. Romano S, Mitro N, Diviccaro S, Spezzano R, Audano M, Garcia-Segura LM, Caruso D,
Melcangi RC. “Short-term effects of diabetes on neurosteroidogenesis in the rat
hippocampus”.JournalofSteroidBiochememistryandMolecularBiology.2017Mar;167:135143.
4. Ferrari A, Fiorino E, Longo R, Barilla S, Mitro N, Cermenati G, Giudici M, Caruso D, Mai A,
Guerrini U, De Fabiani E, Crestani M. “Attenuation of diet-induced obesity and induction of
whitefatbrowningwithachemicalinhibitorofhistonedeacetylases”.InternationalJournalof
Obesity(Lond).2017Feb;41(2):289-298.
5. S. DellaTorre, N. Mitro, R. Fontana, M. Gomaraschi, E. Favari, C. Recordati, F. Lolli, F.
Quagliarini,C.Meda,C.Ohlsson,M.Crestani,N.H.Uhlenhaut,L.CalabresiandA.Maggi.“An
EssentialRoleforLiverERainCouplingHepaticMetabolismtotheReproductiveCycle”.Cell
Reports2016,2016Apr12;15(2):360-71.
6. Dinamarca MC, Guzzetti F, Karpova A, Lim D, Mitro N, Musardo S, Mellone M, Marcello E,
StanicJ,SamaddarT,BurguièreA,CaldarelliA,GenazzaniAA,PerroyJ,FagniL,CanonicoPL,
KreutzMR,GardoniF,DiLucaM.“Ringfingerprotein10isanovelsynaptonuclearmessenger
encodingactivationofNMDAreceptorsinhippocampus”.Elife2016Mar15;5.pii:e12430.
7. G.Cermenati,M.Audano,S.Giatti,V.Carozzi, C.Porretta-Serapiglia,E.Pettinato,C.Ferri,M.
D’Antonio,E.DeFabiani,M.Crestani,S.Scurati,E.Saez,I.Azcoitia,G.Cavaletti,L.M.GarciaSegura, R. C. Melcangi, D. Caruso and N. Mitro. “Lack of Sterol Regulatory Element Binding
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8.
9.
Factor-1c imposes glial fatty acid utilization leading to peripheral neuropathy”. Cell
Metabolism,2015Apr7;21(4):571-83.
Di Gregorio E., Borroni B., Giorgio E., Lacerenza D., Ferrero M., Lo Buono N., Ragusa N.,
Mancini C., Gaussen M., Calcia A., Mitro N., Hoxha E., Mura I., Coviello D.A., Moon Y.A.,
Tesson C., Vaula G., Couarch P., Orsi L., Duregon E., Papotti M.G., Deleuze J.F., Imbert J.,
CostanziC.,PadovaniA.,GiuntiP.,Maillet-VioudM.,DurrA.,BriceA.,TempiaF.,FunaroA.,
BocconeL.,CarusoD.,StevaninG.,BruscoA.“ELOVL5MutationsCauseSpinocerebellarAtaxia
38”.AmericanJournalofHumanGenetics,2014Aug7;95(2):209-17.
Galmozzi A.*, Mitro N.*, Ferrari A., Gers E., Gilardi F., Godio C., Cermenati G., Gualerzi A.,
DonettiE.,RotiliD.,ValenteS.,GuerriniU.,CarusoD.,MaiA.,SaezE.,DeFabianiE.,Crestani
M.“InhibitionofclassIhistonedeacetylasesunveilsamitochondrialsignatureandenhances
oxidative metabolism in skeletal muscle and adipose tissue”. Diabetes. 2013 Mar;62(3):73242.*Equallycontributed.
10. Cermenati G., Abbiati F., Cermenati S., Brioschi E., Volonterio A., Cavaletti G., Saez E., De
Fabiani E., Crestani M., Garcia-Segura L.M., Melcangi R.C., Caruso D, Mitro N. “Diabetesinducedmyelinabnormalitiesareassociatedwithanalteredlipidpattern:protectiveeffects
ofLXRactivation”.TheJournalofLipidResearch,2012Feb;53(2):300-10.
11. CermenatiG.,GiattiS.,CavalettiG.,BianchiR.,MaschiO.,PesaresiM.,AbbiatiF.,Volonterio
A., Saez E., Caruso D., Melcangi R.C., Mitro N. “Activation of the Liver X Receptor increases
neuroactive steroid levels and protects from diabetes-induced peripheral neuropathy”. The
JournalofNeuroscience,2010Sep.8,30(36):11896–11901.
12. Wu C., Delano D.L., Mitro N., Su S.V., Janes J., McClurg P., Batalov S., Welch G.L., Zhang J.,
OrthA.P.,WalkerJ.R.,GlynneR.J.,CookeM.P.,TakahashiJ.S.,ShimomuraK.,KohsakaA.,Bass
J.,SaezE.,WiltshireT.,SuA.I.“GenesetenrichmentineQTLdataidentifiesnovelannotations
andpathwayregulators”.PLoSGenetics,2008May9;4(5):e1000070.
13. SironiL.*,MitroN.*,CiminoM.,GelosaP.,GuerriniU.,TremoliE.andSaezE.“Treatmentwith
LXRagonistsafterfocalcerebralischemiapreventsbraindamage”.FEBSLetters,2008Oct.15;
582(23-24):3396-400.*Equallycontributed.
14. N.Mitro,P.A.Mak,L.Vargas,C.Godio,E.Hampton,V.Molteni,A.KreuschandE.Saez.“The
nuclearreceptorLXRisaglucosesensor”.Nature,2007Jan.11;445(7124):219-223.
15. Mitro N., Vargas L., Romeo R., Koder A. and Saez E. “T0901317 is a potent PXR ligand:
ImplicationsforthebiologyascribedtoLXR”.FEBSLetters,2007May1;581(9):1721-6.
16. Waki H., Park K.W., Mitro N., Pei L,, Damoiseaux R., Wilpitz D.C., Reue K., Saez E. and
Tontonoz P. “The small molecule harmine is an antidiabetic cell-type-specific regulator of
PPARgammaexpression”.CellMetabolism,2007May;5(5):357-70.
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NicoMitroCurriculumVitae
17. N.Mitro,C.Godio,E.DeFabiani,E.Scotti,A.Galmozzi,F.Gilardi,D.Caruso,A.B.VigilChacon,
andM.Crestani.“Insightsintheregulationofcholesterol7α-hydroxylasegenerevealatarget
formodulatingbileacidsynthesis”.Hepatology,2007Sep.;46(3):885-97.
18. E.DeFabiani*,N.Mitro*,F.Gilardi,D.Caruso,G.GalliandM.Crestani.“Coordinatedcontrol
of cholesterol catabolism to bile acids and of gluconeogenesis via a novel mechanism of
transcriptionregulationlinkedtothefasted-to-fedcycle”.TheJournalofBiologicalChemistry,
2003Oct.3;278(40):39124-39132.*Equallycontributed.
PRESENTANDFUTURESCIENTIFICACTIVITIES
Thegeneralresearchgoalofmygroupistoidentifythebiochemicalpathwaysalteredin
age-relatedpathologies.Byintegratinghigh-throughputgenomicscreenings,transcriptomicsand
metabolomics,weaimtofindkeybiochemicalregulatorsthatcanbeinnovativeattractivetargets
todevelopinterventions.Thefollowingaretheongoingresearchlinesinmylaboratory:
1.Diabetesandperipheralneuropathy.
Diabetes is now one of the most common diseases; in fact, over the past 20 years the
number of people affected this pathology has increased exponentially in developed and
developingcountries,becauseofchangesindietaryhabitsandlifestyle.Peripheralneuropathyis
a common complication of diabetes, which severely compromises the quality of life of patients.
Diabeticperipheralneuropathy(DPN)isassociatedwithdeleteriouschangesinperipheralnerves,
suchasmyelindamageanddecreaseinnerveconductionvelocity.
Changes in myelin fatty acid composition have been associated with peripheral
neuropathy,butthespecificroleofperipheralnervefattyacidsynthesisinmyelinformationand
functionispoorlyunderstood.WehavefoundthatmicelackingSREBP-1chavebluntedperipheral
nerve fatty acid synthesis that results in development of peripheral neuropathy. SREBP-1c null
micedevelopedRemakbundlealterationsandhypermyelinationofsmallcaliberfibersthatimpair
nerve function. Peripheral nerves lacking SREBP-1c showed decreased fatty acid synthesis and
glycolytic flux, but increased fatty acid catabolism and mitochondrial function. These metabolic
alterationsweretheresultoflocalaccumulationoftwoendogenousPPARαligands,1-palmitoyl-2oleyl-sn-glycerol-3-phosphatidylcholine and 1-stearoyl-2-oleyl-sn-glycerol-3-phosphatidylcholine.
Treatment with a PPARα antagonist rescued the neuropathy of SREBP-1c null mice. The
integration of genetics, transcriptomics and metabolomics reveals the importance of peripheral
nervefattyacidsynthesistosustainmyelinstructureandfunction(CellMetabolism,2015).
Currently, we are investigating the genome-wide occupancy of SREBP-1c in a diabetic
setting, by means of chromatin immunoprecipitation coupled to DNA sequencing (ChIPseq). The
data obtained by these analyses will be complemented with gene expression profile and
metabolomics.Sincetheintegrationoftranscriptional,regulatoryandmetabolomicdatahasbeen
proven to be more effective than enrichment analyses in the identification of key pathways
altered in a set of biological conditions this research line will probably unravel new markers
associatedtothedevelopmentofdiabetes-inducedperipheralneuropathy.
2.Identificationofnewmitochondrialregulators.
Mitochondria are organelles in human and animal cells where many vital biochemical
reactions take place. They use reducing metabolites and coenzymes, such as NADH and FADH2,
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which are derived from metabolic processes, to execute oxidative phosphorylation (OxPHOS)
throughtheelectrontransportchain(ETC)andgenerateATPtosupportvariouscellularfunctions.
At the same time, reactive oxygen species (ROS), by-products of mitochondrial respiration, are
producedandmaydamagevariouscomponentsincells.Thus,mitochondriaareessentialforthe
maintenanceofnormalphysiologicalfunctionsoftissuecells.
Mitochondrial diseases are a group of disorders caused by dysfunctional mitochondria.
However, dysfunctional mitochondria are also associated with different human diseases such as
neurodegenerative and cardiovascular disorders, obesity, diabetes, cancer, and aging.
Mitochondrial disorders may originate with mutations, acquired or inherited, in mitochondrial
DNA(mtDNA)orinnucleargenesthatcodeformitochondrialcomponents.Theresultingimpaired
ETC and OxPHOS leads to a decrease in cellular energy production (ATP), which is the most
importantandcommoncauseofmitochondrialdysfunctionanddisorders.
Inlinewiththefindingsfromothergroups(Diabetes,2013),werecentlyproposedtheinductionof
mitochondrialbiogenesisasastrategyforthetreatmentofOxPHOSdisordersinamousemodel.
Boosting mitochondrial mass and/or function should increase remaining OxPHOS activity unless
thedefectiveproteinactsasadominant-negative.Otherwise,thisapproachshouldenhancethe
cellularATPsyntheticcapacityandpresumablyimprovecellularenergydeficitsandenhancerepair
mechanisms.
In order to identify genes that modulate mitochondrial function/number, we
overexpressed a cDNA library of 16.000 genes in Hek-293 cells. Individual cDNAs were cotransfected with a luciferase reporter to measure the activity of Tfam promoter, a protein
essentialformitochondrialbiogenesis.Factorsknowntoinfluencemitochondrialnumber/function
appeartomodulateTfamexpressionlevels;evaluationofTfampromoteractivitycanserveasa
markertoidentifygeneticpathwaysthatregulatemitochondrialbiogenesis.
Thegenomichighthroughputscreening(HTS)yielded440clonesabletoinduceorreduceTfam
promoteractivity.AllhitsfromthecDNAscreenwereconfirmedinHek-293cellsbyFACSanalysis
of cells transfected with a single cDNA and stained with markers for mitochondrial density and
function,respectively(secondaryscreening).Positive(126)andnegative(32)hitswereconfirmed
to modulate mitochondrial function/density better than the positive (Pgc-1α) and negative
(p160MybBP)controls.Outofthe126positiveand32negativehits,72and22hits,respectively,
have never been associated with mitochondrial biology. We characterized the positive hit, zinc
finger CCCH-type containing 10 (Zc3h10). Zc3h10 is a functionally unannotated protein that has
neverbeenassociatedtomitochondrialbiology.Togainfurtherinsightsintoitsfunctionalrole,we
investigated Zc3h10 in C2C12 cells, a biological system characterized by physiological
mitochondrial biogenesis (i.e., myoblasts differentiating to myotubes). By using transcriptomics,
metabolomics and RNA immunoprecipitation coupled to sequencing we found that Zc3h10
regulates mitochondrial function and impacts skeletal muscle differentiation by specifically
modulating iron metabolism, a fundamental ion for mitochondrial function (manuscript in
preparation).
Currently, we are generating Zc3h10 conditional knock out in skeletal muscle to prove in
vivoitsroleasnovelmitochondrialregulatorandtoevaluateifitrepresentsapre-clinicalmodelof
mitochondrialdysfunction.
My future research activities will be focused on the understanding of the molecular,
biochemical and metabolic pathways altered in age-related pathologies. In particular, we will
focusourattentionondiabetesanditscomplicationsandonthebiologyofmitochondriathatare
strongly affected during age and age associated diseases (i.e. neurodegenerative diseases and
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others).Mylaboratoryhasextensiveknowledgeinbiochemistry,geneexpressionregulationand
metabolomics;therefore,itrepresentsauniquecombinationtosolvescientificquestionsrelated
tophysiologyandpathophysiology.
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PARTECIPATIONTONATIONALANDINTERNATIONALMEETINGS:
Dr.Mitroparticipatedtomorethan40nationalandinternationalmeetings.
Invitedspeakertointernationalmeetings:
1.
“Bile acids: from “simple” detergents to key regulators of lipid metabolism”. 6th
International Symposium on Global Risk of Coronary Heart Disease and Stroke: Assessment,
PreventionandTreatment,Firenze,12-15June2002.
2.
“Functionalinteractionbetweenhepatocytenuclearfactor-4(HNF-4)andNF-kBregulates
thetranscriptionofcholesterol7α-hydroxylasegene(CYP7A1)”.NuclearReceptorsmeeting2002,
Stockholm,25-28August2002.
3.
“A novel mechanism of transcription regulation in bile acid synthesis and in
gluconeogenesis via dissociation of nuclear receptor/coactivator complex”. EMBO Conference –
BiologyofNuclearReceptors,VillefranchesurMer,4-7June2003.
4.
“Bile acid-mediated dissociation of HNF-4/coactivator complex determines for the
repression of key genes in cholesterol and glucose metabolism”. Keystone Symposia Nuclear
Receptors:OrphanBrothers,Keystone,February28-March4,2004.
5.
“Bileacidsrepresscholesterol7alpha-hydroxylasegenetranscriptionbyrecruitinghistone
deacetylase”.15thInternationalSymposiumonDrugsAffectingLipidMetabolism,Venezia,24-27
October2004.
6.
“Fromnuclearreceptorstochromatin:discoveryofhistonedeacetylasesasnewtargetsof
hypolipidemicdrugs”.MonteCarlo,19-23March2005.
7.
“Identification of histone deacetylase 7 as a new target for treatment of
hypercholesterolemia”.XVIInternationalSymposiumonAtherosclerosis,Rome,June18-22,2006.
8.
“The nuclear receptor LXR is a glucose sensor”. The Meeting of LA area Receptor labs
(MoLAR),CityofHope,Duarte,CA,USA,20April2007.
9.
“The nuclear receptor LXR is a glucose sensor”. Keystone Symposia on Molecular and
Cellular Biology. Nuclear Receptors: Orphan Brothers, Whistler, British Columbia (Canada), 30
March–4April2008.
10. “LiverXReceptoractivationaffectstestosteroneanditsmetabolitelevelsinsciaticnerves
of adult male rats”. EMBO conference on Nuclear Receptors, Cavtat/Dubrovnik (Croazia),
September25-29,2009.
11. “Changing the binding mode to peroxisome proliferator activated receptor (PPAR)
alpha/gamma: a new ligand with improved antidiabetic and antiobesity properties”. American
SocietyforBiochemistryandMolecularBiology(ASBMB)AnnualMeeting,Anaheim,CA,USA,April
24-28,2010.
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12. “Liver x receptors activation ameliorate diabetic peripheral neuropathy increasing locally
neuroactivesteroidlevels".6thInternationalMeeting:SteroidsandNervousSystem,Torino,Italy,
February19-23,2011.
13. “Liver X Receptor activation protects from diabetic neuropathy by restoring fatty acid
biosynthesis"36thFEBSmeeting,Torino,Italy,June25-30,2011.
14. “LXR-mediatedlipogenesisprotectsperipheralnervesfromtypeIdiabetes-inducedmyelin
alterations”.EMBOConferenceonNuclearReceptors:FromMolecularMechanismtoHealthand
Disease,Sitges,Barcelona(Spain),September16-20.
15. “Identification of novel regulators of mitochondrial function by functional genomic
screening" International Summer School Program on Chemical and genomics-based strategies in
thediscoveryofnoveldrugtargets,Bologna,Italy25-29June2012.
16. “LXR-mediated lipogenesis protects peripheral nerves from diabetes-induced myelin
alterations”.The35thEuropeanLipoproteinClub(ELC),Tutzing(Germany),September10-13.
17. “Gain of function genomic screening to unveil signaling pathways that control
mitochondrial biogenesis”. International Summer School Program on Chemical and genomicsbasedstrategiesinthediscoveryofnoveldrugtargets,Bologna,Italy23-27June2013.
18. “High throughput screening approach to identify new mitochondrial regulators”.
International Summer School Program on Chemical and genomics-based strategies in the
discoveryofnoveldrugtargets,Bologna,Italy24-27June2014.
19. “A new pathway to exert protective effects on diabetes peripheral neuropathy: role of
neuroactive steroids on myelin lipid profile” 8th International Meeting: Steroids and Nervous
System,Torino,Italy,February14-18,2015.
20. “The lipogenic regulator Sterol Regulatory Element Binding Factor-1c is required to
maintain peripheral nerve structure and function”. European Society for Neurochemistry's
ConferenceMolecularMechanismsofRegulationintheNervousSystem,June14-17,2015Tartu,
Estonia.
21. “Integrationoftranscriptomicsandmetabolomicsforthestudyofperipheralneuropathy”,
9°MSPharmaDay,May25-27,2016SantaPalomba,Roma,Italy.
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“Identificationandcharacterizationofanovelmitochondrialregulator”.GiovanniArmenise
HarvardFoundation16thAnnualSymposium:FromMolecularMechanismstoPrecisionMedicine,
June26-29,2016Gubbio,Italy.
Invitedorselectedspeakerinnationalmeetings:
1.
“Bile acid and tumor necrosis factor-α signalings negatively affect the transcription of
cholesterol7alpha-hydroxylasegene(CYP7A1)byconvergingtohepatocytenuclearfactor-4(HNF8
NicoMitroCurriculumVitae
4)”.2001MeetingoftheItalianSocietyforBiochemistryandMolecularBiology(SIB),LombardyLiguria-Piedmontsections,Pavia,May18,2001.
2.
“Role of Liver X Receptor (LXR) in gene expression regulation mediated by oxysterols in
oxidizedLDLandinatheroscleroticplaque”.1stStudydayon“EndotheliumandAtherosclerosis”,
Milano,October6,2001.
3.
“Expressionprofileofgenesinvolvedincholesterolmetabolism”.1stMeetingontheReal
TimePCR,Milano,June5,2002.
4.
“Receptor/Coactivator dissociation detemines the inhibitory effect of bile acids on the
transcriptionofcholesterol7-alphahydroxylase(CYP7A1)gene”.2ndStudydayon“Endothelium
andAtherosclerosis”,Milano,October5,2002.
5.
“Expressionprofileofgenesinvolvedincholesterolmetabolism”.2ndMeetingontheReal
TimePCR,Milano,June26,2003.
6.
“Molecularmechanismsofthegluconeogenesisandofcholesterolcatabolismastargetfor
newtherapiesfordiabetesandhypercholesterolemia”.3rdStudydayon"GlobalCardiovascular
risk:roleofmetabolicsyndrome”.Milano,October4,2003.
7.
“HNF-4α /Coactivator dissociation detemines the inhibitory effect of bile acids on the
transcription of genes involved in glucose and cholesterol metabolism”. 2004 Meeting of the
ItalianSocietyforBiochemistryandMolecularBiology(SIB),Lombardy-Liguria-Piedmontsections,
Novara,May14,2004.
8.
“From gene transcription to therapy: discovery of novel molecular target for effective
treatmentofhypercholesterolemia”.49thNationalMeetingoftheItalianSocietyforBiochemistry
andMolecularBiology(SIB),Riccione,September28–October1,2004.
9.
“Regulation of hepatic lipid and carbohydrate metabolism by the Liver X Receptor”. 53°
National Meeting of the Italian Society for Biochemistry and Molecular Biology (SIB), Riccione,
September26-28,2008.
10. “GlucoseandlipidmetabolismregulationbyLiverXReceptor(LXR)”.8thStudydayofthe
Italian Society for the Study of Atherosclerosis (SISA) " Base and clinical research in the
cardiovasculararea”,Milano,October4,2008.
11. “GlucoseandlipidmetabolismregulationbyLiverXReceptor(LXR)”.RegionalMeetingof
ItalianSocietyfortheStudyofAtherosclerosis(SISA)"Nutritionandcardiovascularpathologies–
prevention:fromnutrientstodrugs”,Moltrasio(Como),March6-7,2009.
12. “From crystal structure to biological activity: design and characterization of a novel dual
peroxysome proliferator activated receptor alpha/gamma agonist with potent antidiabetic and
antiobesityactivity”.54thNationalMeetingoftheItalianSocietyofBiochemistryandMolecular
Biology(SIB),Catania,September23–27,2009.
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13. “Antidiabetic and antiobesity activity of a novel dual peroxysome proliferator activated
receptor alpha/gamma ligand”. 23rd National Congress of the Italian Society for the Study of
Atherosclerosis(SISA),Roma,November25-28,2009.
14. “Liver X Receptor activation reduces the severity of peripheral neuropathy by diabetes
increasing locally neuroactive steroid levels”. Italian group of neuroendocrine sciences (GISNe),
Milano,May7-8,2010.
15. “LiverXReceptorsactivationamelioratesdiabeticperipheralneuropathyincreasinglocally
neuroactive steroid levels”. 2010 Meeting of the Italian Society for Biochemistry and Molecular
Biology(SIB),Lombardy-Liguria-Piedmontsections,Varese,May28,2010.
16. “RoleofLXRindiabetescomplications”.DiabetesandHDL:RoleofnuclearreceptorsLXR.
S.ElisabettaCenter,UniversityofParmaCampus,Parma,June25,2010.
17. “RoleofLiverXReceptors(LXRs)inthedevelopmentofatherosclerosisintype2diabetes”.
55thNationalMeetingoftheItalianSocietyofBiochemistryandMolecularBiology(SIB),Milano,
September14-17,2010.
18. “Integratedapproachesforthefunctionalvalidationofpotentialmitochondrialregulators”.
56thNationalMeetingoftheItalianSocietyofBiochemistryandMolecularBiology(SIB),Chieti,26
-29settembre2012.
19. “Neuroactive Steroids and Diabetic Neuropathy”. 15th National meeting of the Italian
SocietyofNeuroscience(SINS),Roma,October3-5,2013.
20. From power to dysfunction: a new mitochondrial regulator for diabetes? Spring Meeting
SISA,SIIA,SIMI,Roma,April7-8,2017.
SCIENTIFICPARTECIPATIONININTERNATIONALANDNATIONALRESEARCHPROJECTS,SELECTED
FORFUNDINGBASEDONCALLSTHATINVOLVEDCOMPETITIVEPEERREVIEW
Dr. Mitro has contributed to obtain several funded projects as principal investigator and
coordinatorasdetailedbelow:
2007-2008 American Heart Association post-doctoral fellowship. “The oxysterol receptor LXR
asalinkbetweenatherosclerosisandhyperglycemia.”
2008-2011 Giovanni Armenise-Harvard Foundation Career Development Award.
“Transcriptionallinkbetweendiabetesandatherosclerosis.”
2011-2013 Giovanni Armenise-Harvard Foundation Career Development Award. “Role of LXR
onsciaticnervemyelinlipidcompositionindiabetes.”
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2014-2017 Italian Ministry of Health – Young Researcher Call 2011. “Investigating the
adaptive/maladaptive balance in ER-stress response: insights from a CMT1B neuropathy mouse
model”.
2014-2017 Italian Ministry of Health – Young Researcher Call 2011. “Dissecting the role of
Peroxisome Proliferator Activated Receptor gamma coactivator-1alpha (PGC-1α) and adipose
triglyceridelipase(ATGL)inParkinson”.
2015-2018 Cariplo Foundation, Young Biomedicine Researcher Call 2014. “Impact of blunted
fattyacidsynthesisonthedevelopmentofdiabeticperipheralneuropathy:decipheringtheroleof
thelipogenicfactorSREBP-1c”.
Dr.Mitroalsoparticipatedinthefollowingfundedprojects:
2001-2004 CEE Project, V Framework Programme. “Studies on the role of orphan nuclear
receptors on cholesterol catabolism as new pharmacological targets for cardiovascular diseases
andsearchforligandsmodulatingtheiractivityNORThQLG1-CT2001-01513.”Coordinator:Prof.
MaurizioCrestani.
2002 Italian Ministry of the Education, University and Research (MIUR) –FIRST CALL. “Oxidized
cholesterol products: modulation of their synthesis by physiological stimuli and by natural
antioxidantsandeffectsongeneexpression”.Coordinator:Prof.GiovanniGalli.
2002-2004 ItalianMinistryoftheEducation,UniversityandResearch(MIUR)–PRINCALL20022004. “Regulation of the cholesterol 7alpha-hydroxylase (CYP7A1) gene by bile acids: role of
transcriptional coactivators and chromatin in experimental animal models”. Coordinator: Dr.ssa
EmmaDeFabiani.
2004 Italian Ministry of the Education, University and Research (MIUR) –FIRST CALL.
“Transcriptional regulation in hepatic metabolism and in vascular wall from physiological stimuli
andnutrients”.Coordinator:Prof.DonatellaCaruso.
2004-2006 ItalianMinistryoftheEducation,UniversityandResearch(MIUR)–PRINCALL20042006.“Globalgenomicapproachtostudythebileacidregulationonrelevantgenesinmetabolic
syndrome”.CoordinatorProf.MaurizioCrestani.
2004-2006 TELETHON Foundation Project. “Inhibition of histone deacetylase as a novel
approachforthetherapyofmonogenicfamilialhypercholesterolemiaandthepreventionofthe
associatedprematurecoronaryarterydisease.”Coordinator:Prof.MaurizioCrestani.
2005-2007 ItalianMinistryoftheEducation,UniversityandResearch(MIUR)–PRINCALL20052007.“Design,synthesisandpharmacologicalevaluationofanti-atheroscleroticandhypolipidemic
drugs.”Coordinator:Prof.ErmannoValoti.
2005-2007 ItalianMinistryoftheEducation,UniversityandResearch(MIUR)–PRINCALL20052007. “Clamidie and Spirochete infections: effects on hepatic lipid metabolism and role of
macrophageactivation.”Coordinator:Dr.MicheleMortarino.
11
NicoMitroCurriculumVitae
2006-2010 VI Framework Programme. “Application-oriented studies on regulatory networks
involved in lipid homeostasis and atherosclerosis.” SOUTH LSHM-CT 2006-037498. Coordinator:
Prof.MaurizioCrestani.
2008-2012 Cariplo Foundation – Biomedicine Call. “Histone Deacetylases in the
pathophysiologyoflipidmetabolism:multidisciplinaryapproachesincellularandanimalmodels”.
Coordinator:Prof.MaurizioCrestani.
2012-2015 Cariplo Foundation – Biomedicine Call. “Diabetic peripheral neuropathy:
relationshipsbetweenneuroactivesteroidsandmyelinlipidsynthesis”.Coordinator:Prof.Roberto
C.Melcangi.
2013-2018 VII Framework Programme. “Health and the Understanding of Metabolism, Aging
andNutrition”.Coordinator:Prof.MaurizioCrestani.
2014-2017 TELETHONFoundationProject.“Translatingmolecularpathologyintoatherapeutic
strategyinSCA38,anewlyidentifiedformofspinocerebellarataxia”Coordinator:Prof.Donatella
Caruso.
PATENTS:
Dr.Mitroisco-inventorandownerofthefollowingpatents:
1. "New synthetic ligands of PPARalpha and gamma for the therapy of diabetes and
atherosclerosis"(2004).Patentnumber:MI2004A000405.
2. "A new hypocholesterolemic approach mediated by the upregulation of cholesterol 7-alpha
hydroxylasegenetranscription"(2005).Patentnumber:WO2005105066.
REVIEWERFORMANUSCRIPTANDPROJECTEVALUATION
Dr.Mitroisad-hocreviewerforthefollowinginternationaljournals:
Journal of Endocrinological Investigation, Frontiers in Neuroscience, Journal of Agricultural and
Food Chemistry, British Journal of Nutrition, Cell Biochemistry & Function, Journal of
Neuroendocrinology,PharmacologicalResearch,Endocrine,PLOSOne,NeurobiologyofDisease,
FEBSLetters,MolecularandCellularBiology,JournalofLipidResearch,BiochimicaetBiophysica
Acta(BBA)-MolecularandCellBiologyofLipids,NatureCommunications.
Dr.Mitroisalsoad-hocreviewerforthefollowingfundingagencies:
Italian Ministry of the Education, University and Research (MIUR), Diabetes UK and French
NationalResearchAgency.
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NicoMitroCurriculumVitae
SCIENTIFICAWARD
CareerAwards
10/2003
Best Young Researcher for Atherosclerosis. Italian Society for the Study of
Atherosclerosis(SISA).
05/2006
Best Researcher of the Year of the Genomics Institute of the Novartis Research
Foundation.
10/2008
Best Young Researcher “Giovanni Galli” Prize for the Study of Atherosclerosis.
ItalianSocietyfortheStudyofAtherosclerosis(SISA).
10/2009
GalenoPrizeasBestYoungInvestigator.
06/2015
Young Scientist Lecture Award at the European Society for Neurochemistry's
Conference Molecular Mechanisms of Regulation in the Nervous System
June14-17,2015inTartu,Estonia.
BestAbstractAward
9/2004
Best Poster at the 49th National Meeting of the Italian Society for Biochemistry
andMolecularBiology(SIB),Riccione,September28–October1,2004.
10/2004
“Young Researcher Award” at the 15th International Symposium on Drugs
AffectingLipidMetabolism(DALM),Venezia,October24-27,2004.
11/2009
Astrazeneca Award as best oral presentation at 23rd National Congress of the
Italian Society for the Study of Atherosclerosis (SISA). Roma, November 25-28
novembre2009.
09/2010
Bestposteratthe55thNationalMeetingoftheItalianSocietyofBiochemistryand
MolecularBiology(SIB),Milano,September14-17,2010.
Fellowship
02/2010
FellowshipfromtheItalianSocietyofBiochemistryandMolecularBiology(SIB)to
spentabriefresearchperiodatTheScrippsResearchInstitute,LaJolla,CA,USA.
04/2010
Postdoctoral travel fellowship from the American Society for Biochemistry and
MolecularBiology(ASBMB)toattendtheannualmeeting.
11/2010
TravelfellowshiptoattendtheannualmeetingoftheItalianSocietyfortheStudy
ofAtherosclerosis(SISA).
13
NicoMitroCurriculumVitae
MEMBEROFSCIENTIFICSOCIETIES
Dr.Mitroiscurrentlyamemberofthefollowingscientificsocieties:
From2001 Co-FounderoftheNationalAssociationofItalianBiotechnologists(ANBI)
From2001 ItalianSocietyfortheStudyofAtherosclerosis(SISA)
From2004 ItalianSocietyofBiochemistryandMolecularBiology(SIB)
From2004 FederationofEuropeanBiochemicalSocieties(FEBS)
From2012 EuropeanAssociationfortheStudyofDiabetes(EASD)
From2013 ItalianSocietyofNeuroscience(SINS)
From2013 FederationofEuropeanNeuroscienceSocieties(FENS)
From2015 EuropeanSocietyforNeurochemistry(ESN)
From2015 InternationalSocietyforNeurochemistry(ISN)
INSTITUTIONALANDORGANIZINGACTIVITIES
From 2013 Dr. Mitro is a Member of the Doctoral School Committee in Biochemical Sciences of
theUniversityofMilan.
In 2005, Dr. Mitro has been member of the local organizing committee of the 3rd International
SymposiumonPPARsEfficacyandSafety:frombasicsciencetoclinicalapplications,MonteCarlo,
March19-23.
From 2012 Dr. Mitro is a member of the local organizing committee of the Neurosteroids and
NervousSystemInternationalmeeting.
In 2013 Dr. Mitro has been member of the Scientific Committee of the 54th International
ConferenceontheBioscienceofLipids(ICBL),Bari,September17-21.
From 2015 Dr. Mitro is a co-organizer of the Milan area meeting for young biochemists in
GargnanosulGarda,Brescia,Italy.
14
NicoMitroCurriculumVitae
TEACHINGANDTUTORINGACTIVITY
2001-2005and2008-Present
Seminars, lessons and laboratory trainer in Biochemistry,
Applied Biochemistry and Molecular Biology, School of
Pharmacy,UniversityofMilan.
2011-Present
ProfessorofAppliedBiochemistryforthedegreeinchemistry
andpharmaceuticaltechnologyoftheUniversityofMilan.
2014-2015
Professor of Molecular Mechanism and regulation of
Biotransformationsforthedegreeinenvironmentalchemical
andtoxicologicalsafetyandscience.
2015-Present
Professor of Biochemistry for the degree in science and
technologiesofherbalmedicineoftheUniversityofMilan.
2001-2005and2008-Present
Tutor of more than 10 students for their experimental
graduationthesis,UniversityofMilan.
2014-Present
Tutor of a Ph.D. student in Biochemical Sciences of the
UniversityofMilan.
Milan,April13,2017
Signature
15
NICO MITRO, Ph.D.
DIPARTIMENTO DI SCIENZE FARMACOLOGICHE E BIOMOLECOLARI-DiSFeB
UNIVERSITÀ DEGLI STUDI DI MILANO
VIA BALZARETTI, 9
20133, MILANO, ITALY
TEL. (0039) 02-50318253
FAX. (0039) 02-50318391
April 20,2017
To: Dr. Natalia N. Nalivaeva, PhD, DSc
ESN Secretary
Dear Natalia,
Given the nominations sustaining my candidacy to the ESN council, the present letter is to confirm
my willingness to be elected as ESN council member.
Best regards,
Nico Mitro, Ph.D.
Associate Professor of Biochemistry
Dipartimento di Scienze Farmacologiche e Biomolecolari, DiSFeB
Università degli Studi di Milano
1
Natalia Nalivaeva
From:
Sent:
To:
Subject:
Attachments:
Anthony Turner [Professor FBS]
14 April 2017 16:12
Natalia Nalivaeva
nomination of Nico Mitro for ESN Council
CV Nico Mitro .pdf
Dear Natasha, I am formally nominating Dr Nico Mitro as a candidate for ESN Council in the forthcoming election. I believe he is a very suitable candidate having been a previous recipient of an ESN Young Investigator Lectureship Award and someone who has shown a real commitment to ESN activities in recent years. His CV is attached to this letter and he has confirmed to me that he is willing to stand as an ESN Councillor. With best wishes, Tony 1
Natalia Nalivaeva
From:
Sent:
To:
Subject:
Attachments:
donatella <[email protected]>
19 April 2017 16:07
Natalia Nalivaeva
ESN nomination
CV Nico Mitro .pdf
Dear Natalia, you will find enclosed the CV of Nico Mitro. Although young, I think that Nico will be a good candidate for the ESN Council, as you can see from his curriculum . Thank you for the consideration, with best regards Donatella Caruso ‐‐ Donatella Caruso, Ph.D. Full Professor of Biochemistry President of the Mass Spectrometry Division SCI Lab. of Biochemistry & Molecular Biology of Metabolism ‐ Mass Spectrometry "Giovanni Galli" Dept. of Pharmacological and Biomolecular Sciences Via Balzaretti, 9 ‐ 20133 Milan, Italy Tel +39‐02‐50318323‐8344
http://www.researcherid.com/rid/A‐3332‐2014 ‐‐‐ Questa e‐mail è stata controllata per individuare virus con Avast antivirus. https://www.avast.com/antivirus 1