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The Molecular Imager FX
Family of Imagers
™
The Future of Molecular Imaging
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Molecular Imager
FX Systems
Shown here, the Molecular
Imager FX™ Pro Plus multiimager
with the external laser module.
The screen displays a three-color
(two-dye) western blot analyzed
by Quantity One® software.
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SYPRO Ruby
SYPRO Red
SYPRO Orange
The Molecular Imager FX Family
A Versatile Family of Products
The Molecular Imager FX family includes four imaging products:
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Molecular Imager FX Pro Plus multiimager system
Molecular Imager FX™ Pro fluorescent imager
Molecular Imager FX system
Personal Molecular Imager FX™ system
These products’ combination of technological innovations
supports a wide range of imaging applications, including:
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Single- and multicolor fluorescence detection by direct laser
excitation (FX Pro, FX Pro Plus, and FX)
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Radioisotopic imaging using your choice of phosphor screen
(FX Pro Plus, FX, and Personal Molecular Imager FX)
Ultimate Flexibility
Molecular Imager FX instruments give you a choice of the best
technologies for the application — storage phosphor imaging
for rapid, quantitative detection
of radioisotopes, and laser
excitation for rapid and
accurate detection of
fluorescent samples.
External Laser Options
For applications requiring additional laser lines, an optional
external laser module (housing up to three lasers) can be added
for unparalleled fluorescence flexibility. This field upgrade
guarantees that you can change research focus or take advantage
of developments in fluorescent dye technology. All laser
combinations are driven by user-friendly Quantity One software.
Simply choose the application, and the software automatically
selects the optimal hardware (laser and filter) settings. You can
also create custom applications. After an image has been
acquired, quantitate it with powerful Quantity One analysis tools.
User-accessible
filters
Multipurpose sample tray
Shown here is the Molecular
Imager FX system with
optional external laser.
External laser port
Sidemount module
High-resolution
scanhead
Photomultiplier tube
Internal dualwavelength laser
Emission
filter cubes
Excitation
filters
Sample
tray
External
laser port
Optional
external laser
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SYBR Green I
FITC and Texas Red dual fluorescence
Ethidium bromide
Optional external
or sidemount laser.
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Optional external
or sidemount laser.
Molecular Imager FX Pro Plus
MultiImager System
Molecular Imager FX Pro
Fluorescent Imager
This multiimaging system not only detects a wide range of
fluorophores but also can be used with storage phosphor
technology to quantitate radioisotopes. It has 50 µm resolution
and a 5 order of magnitude linear dynamic range.
Optimally suited for the field of proteomics, this fluorescence
scanner has 50 µm resolution and a 5 order of magnitude
linear dynamic range.
Single- and multicolor fluorescence via direct laser excitation
Fluorescence imaging (high sensitivity and high resolution)
Radioisotopic imaging — quantitates 32P, 33P, 35S, 14C, and 3H
Greater dynamic range: 5 orders vs. 3 for X-ray film
Accommodates most commercially available phosphor screens*
(20 x 25 cm and 35 x 43 cm), and large gels up to 35 x 43 cm
Optional external 488 nm and 635 nm lasers
External laser module (housing up to three lasers)
Seamless integration with PDQuest™ 2-D analysis software
16-bit digital resolution
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Fluorescence-only scanner (high sensitivity and high resolution)
Single- and multicolor fluorescence via direct laser excitation
Accommodates large gels up to 35 x 43 cm
Optional external 488 nm and 635 nm lasers
External laser module (housing up to 3 lasers)
Seamless integration with PDQuest 2-D analysis software
Radioisotopes
Fluorescence
Chemifluorescence
Radioisotopes
Fluorescence
Chemifluorescence
Recommended
Not recommended
Fluorescence Imaging
Molecular Imager FX systems permit the detection of
almost any fluorescent dye. The internal and external
laser options allow optimal excitation of single- or
multicolor fluorescent samples. Software-controlled
filter wheels accommodate eight filter slots, allowing
detection of combinations of dyes.
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Cy3/Cy5 FITC western blot
Dual fluorescence high-density array
Radiant® Red fluorescent RNA stain
Optional external
or sidemount laser.
Molecular Imager FX System
Personal Molecular Imager
FX System
The unique FX is the family member that offers the fullest
range of imaging features in a single product. It has 50 µm
resolution and a 5 order of magnitude linear dynamic range for
fluorescence and radioisotopic imaging. You can also image
and accurately quantitate chemiluminescent samples.
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The Personal Molecular Imager is a dedicated isotope imaging
system. It offers radioisotopic imaging via storage phosphor
technology and a 635 nm red diode laser. It has 50 µm resolution
and a 5 order of magnitude linear dynamic range.
Fluorescence imaging (high sensitivity and high resolution)
Radioisotopic imaging — quantitates 32P, 33P, 35S, 14C, and 3H
Greater dynamic range: 5 orders vs. 3 for X-ray film
Accommodates most commercially available phosphor screens*
(20 x 25 cm and 35 x 43 cm)
532 nm/1,064 nm dual-line laser
External laser module (housing up to 3 lasers)
Optional external 488 nm and 635 nm lasers
Seamless integration with PDQuest 2-D analysis software
16-bit digital resolution
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Quantitates 32P, 33P, 35S, 14C, and 3H
Sensitivity at least 10 times greater than X-ray film
Reduced exposure times — 10 to 20 times faster than film!
Immediate digital image — no film or darkroom needed
Accommodates most commercially available phosphor screens*
(20 x 25 cm and 35 x 43 cm)
Radioisotopes
Fluorescence
Chemifluorescence
Radioisotopes
* Compatible phosphor screens include
those manufactured by Kodak, Fuji,
and Molecular Dynamics.
Fluorescence
Chemifluorescence
Storage Phosphor Imaging
The storage phosphor imaging technology of the FX family offers isotopic imaging
with unsurpassed sensitivity, exceptional resolution, and time-saving exposures that
are up to 10 times faster than traditional film techniques. In addition, the products
incorporating this technology offer superior quantitation over a large dynamic range
and can analyze a wide selection of research isotopes.
14
C-labeled rat
32
P-labeled DNA
35
S-labeled proteins
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Specifications
Molecular
Imager
FX Pro Plus
Molecular
Imager
FX Pro
Molecular
Imager
FX
Personal
Molecular
Imager FX
System type
Fluorescent/storage
phosphor multiimager
Fluorescent scanner
Fluorescent/storage
phosphor multiimager
Storage phosphor imager
Excitation
20 mW 532 nm (green),
15 mW 488 nm (blue),
10 mW 635 nm (red) laser
20 mW 532 nm,
15 mW 488 nm,
10 mW 635 nm
20 mW 532/1,064 nm (green/far-red),
15 mW 488 nm,
10 mW 635 nm
10 mW 635 nm
2.5 line pairs/mm for 14C,
1.5 line pairs/mm for 32P
0.15 dpm/mm2 for 32P,
0.95 dpm/mm2 for 14C
N/A
2.5 line pairs/mm for 14C,
1.5 line pairs/mm for 32P
0.15 dpm/mm2 for 32P,
0.95 dpm/mm2 for 14C
2.5 line pairs/mm for 14C,
1.5 line pairs/mm for 32P
0.15 dpm/mm2 for 32P,
0.95 dpm/mm2 for 14C
Radioactivity
Resolution
Detection
N/A
Standard
emission filters
390 nm BP, 526 nm SP,
530 nm BP, 555 nm LP,
640 nm BP
526 nm SP, 530 nm BP,
555 nm LP, 640 nm BP
390 nm BP, 526 nm SP,
530 nm BP, 555 nm LP,
640 nm BP, IR blocking filter
N/A
Optional
emission filters
605 nm BP, 690 nm BP
605 nm BP, 690 nm BP
605 nm BP, 690 nm BP
N/A
Fluorescence
Wavelengths
Resolution
500 –700 nm
5 line pairs/mm
500 –700 nm
5 line pairs/mm
500 –700 nm
5 line pairs/mm
N/A
N/A
Photomultiplier tubes
1
1
1
1
Dynamic range
5 orders of magnitude
(1:100,000)
5 orders of magnitude
(1:100,000)
5 orders of magnitude
(1:100,000)
5 orders of magnitude
(1:100,000)
Sample size
35 x 43 cm
35 x 43 cm
35 x 43 cm
35 x 43 cm
Scan resolution
50, 100, 200, 800 µm
50, 100, 200, 800 µm
50, 100, 200, 800 µm
50, 100, 200, 800 µm
Operating system
Windows NT/98/2000/Me
or Macintosh OS 9
Windows NT/98/2000/Me
or Macintosh OS 9
Windows NT/98/2000/Me
or Macintosh OS 9
Windows NT/98/2000/Me
or Macintosh OS 9
Digital resolution
16-bit
16-bit
16-bit
16-bit
Uniformity
±5%
±5%
±5%
±5%
Scan time
1.5 – 34 min
1.5 – 34 min
1.5 – 34 min
1.5– 34 min
Dimensions (W x D x H)
57 x 68 x 30 cm
57 x 68 x 30 cm
57 x 68 x 30 cm
57 x 68 x 30 cm
Weight (uncrated)
31 kg (68 lb)
31 kg (68 lb)
31 kg (68 lb)
31 kg (68 lb)
Ordering Information
Catalog #
170-7856
170-7857
170-7850
170-7851
170-9400
170-9401
170-7892
170-7890
170-7891
Description
Molecular Imager FX Pro Fluorescent Imager, PC, 110/240 V, 35 x 43 cm scanning
format, includes Quantity One software, sample tray, SCSI cable, instructions
Molecular Imager FX Pro Fluorescent Imager, Mac
Molecular Imager FX Pro Plus MultiImager System, PC, 100/240 V, 35 x 43 cm
scanning format, includes Quantity One software, sample tray, SCSI cable,
instructions
Molecular Imager FX Pro Plus MultiImager System, Mac
Personal Molecular Imager FX System, PC, 100/240 V, 35 x 43 scanning format,
includes Quantity One software, sample tray, SCSI cable, instructions
Personal Molecular Imager FX System, Mac
External Lasers, 488 nm and 635 nm
External Laser, 488 nm
635 nm Sidemount Laser
Cy is a trademark of Amersham Biosciences. Mac and Macintosh are
trademarks of Apple Computer. SYBR, SYPRO, and Texas Red are
trademarks of Molecular Probes, Inc. Windows and Windows NT, 98,
2000, and Me are trademarks of Microsoft Corp.
Molecular Imager FX systems are covered by the following patents:
US patents 4,812,660, 4,822,520, and 4,830,875 (exclusively licensed
to Bio-Rad Laboratories); US patent 5,266,803 (issued to Bio-Rad);
and patents pending.
Bio-Rad
Laboratories, Inc.
Web site www.bio-rad.com USA (800) 4BIORAD Australia 02 9914 2800 Austria (01)-877 89 01 Belgium 09-385 55 11 Brazil 55 21 2527 3454
Canada (905) 712-2771 China (86-21) 63052255 Czech Republic + 420 2 41 43 05 32 Denmark 44 52 10 00 Finland 09 804 22 00
France 01 47 95 69 65 Germany 089 318 84-0 Hong Kong 852-2789-3300 Hungary 36 1 455 8800 India (91-124)-6398112/113/114, 6450092/93
Israel 03 951 4127 Italy 39 02 216091 Japan 03-5811-6270 Korea 82-2-3473-4460 Latin America 305-894-5950 Mexico 55-52-00-05-20
The Netherlands 0318-540666 New Zealand 64 9 415 2280 Norway 23 38 41 30 Poland + 48 22 331 99 99 Portugal 351-21-472-7700
Russia 7 095 721 1404 Singapore 65-6415 3188 South Africa 00 27 11 4428508 Spain 34 91 590 5200 Sweden 08 555 12700
Switzerland 061 717-9555 Taiwan (8862) 2578-7189/2578-7241 United Kingdom 020 8328 2000
Life Science
Group
Bulletin 2737
US/EG
Rev B
03-861
1203
Sig 1103