Version: 1
Revision Date: 05/25/2016
Human TBNK Cocktail
Catalog# / Size
391501 / 50 tests
Clone
SK7/5.1H11/B73.1/2D1/SJ25C1 (See other available
formats)
Isotype
Mouse IgG1, κ (all clones)
Ave. Rating
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Description
CD3 (Clone SK7): The CD3/T-cell receptor (TCR) complex is composed of
two CD3ε, one CD3γ, one CD3δ, one CD3ζ (CD247), and a T-cell receptor
(α/β or γ/δ) heterodimer. It is found on all mature T cells, NK T cells, and
some thymocytes. It plays a role in antigen recognition, signal transduction,
and T cell activation.
CD45 (Clone 2D1): It is a type I membrane glycoprotein also known as
leukocyte common antigen (LCA) and T200. It functions as a tyrosine
phosphatase expressed on the plasma membrane of all hematopoietic cells,
except erythrocytes or platelets. CD45 regulates a variety of cellular
processes including cell growth, differentiation, cell cycle, and oncogenic
transformation.
CD56 (Clone 5.1H11): A single transmembrane glycoprotein, also known as
NCAM (neural cell adhesion molecule), Leu-19, or NKH1. It is expressed on
NK and NKT cells, but in other cells and tissues as well, such as large
granular lymphocyte (LGL) leukemias, small-cell lung carcinomas, neuronal
derived tumors, myelomas, myeloid leukemias, brain cerebellum and cortex,
and neuromuscular junctions. CD56 plays a role in homophilic and
heterophilic adhesion via binding to itself or heparan sulfate.
CD16 (Clone B73.1): Also known as low affinity IgG receptor III (FcγRIII), it is
expressed as two distinct forms (CD16a and CD16b). CD16a (FcγRIIIA) is
expressed on the surface of NK cells, activated monocytes, macrophages, a
subset of T cells, and placental trophoblasts in humans. CD16b (FcγRIIIB) is
expressed specifically on neutrophils. CD16 binds aggregated IgG or IgGantigen complex which functions in NK cell activation, phagocytosis, and
antibody-dependent cell-mediated cytotoxicity (ADCC).
CD19 (Clone SJ25C1): Also known as B4, it is a member of the
immunoglobulin superfamily expressed on B cells (from pro-B to blastoid B
cells, absent on plasma cells) and follicular dendritic cells. CD19 is involved
in B cell development, activation, and differentiation. CD19 forms a
complex with CD21 (CR2) and CD81 (TAPA-1), and functions as a BCR coreceptor.
Reactivity
Human
Human peripheral blood was stained with
Human TBNK Cocktail. Middle and bottom
panel are gated on CD45 + lymphocytes.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA
(origin USA).
Preparation
This reagent is a combination of FITC conjugated SK7, PE conjugated 5.1H11, PE conjugated
B73.1, PerCP conjugated 2D1, and APC conjugated SJ25C1.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our
Lookup tool if you have a lot number.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from
prolonged exposure to light. Do not freeze.
Application
FC - Quality tested
Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow
cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 20 µl per
million cells or 20 µl per 100 µl of whole blood.
Application Notes
This cocktail is intended to differentiate and determine the frequencies of T-Cells, B-Cells, NKCells and NKT-Cells in a single test format using either lyse-wash or lyse-no wash human
peripheral blood assays. It may be used as a stand-alone product or as a common marker
backbone to which additional antibodies may be added for specific applications.
Note: Isotype controls are not included. Single color compensation controls are not included.
RRID
AB_2616702 (BioLegend Cat. No. 391501)
Antigen Details
Distribution
T-cells, B-cells, NK-cells and NKT-cells.
Antigen References
1. Tedder T, et al. 1994. Immunol. Today 15:437.
2. Bradbury L, et al. 1993. J. Immunol. 151:2915.
3. Barclay N, et al. 1993. The Leucocyte Antigen FactsBook. Academic Press. San Diego.
4. Beverley P, et al. 1981. Eur. J. Immunol. 11:329.
5. Lanier L, et al. 1986. J. Immunol. 137:2501.
6. Schubert J, et al. 1989. In Leucocyte Typing IV (Knapp W, ed) Oxford University Press Oxford
pp 711.
7. Palmer BE, et al. 2005. J. Immunol. 175:8415.
8. Schachner M, Martini R. 1995. Trends Neurosci. 18:183.
9. Wood KL, et al. 2005. Clin. Immunol. 117:294.
10. Björkström NK, et al. 2008. J. Immonol. 181:4219.
11. Lanier L, et al. 1991. J. Immunol. 146:4421.
12. Hemperly J, et al. 1990. J. Mol. Neurosci. 2:71.
13. Cremer H, et al. 1994. Nature 367:455.
14. Bradstock KF, et al. 1980. J. Natl. Cancer Inst. 65:33.
15. Csiba A, et al. 1984. Br. J. Cancer 50:699.
16. Tchilian EZ, et al. 2001. J. Immunol. 166:1308.
17. Lee MS, et al. 2004. Int. Immunol. 16:1109.
Gene ID
916
4684
2214
2215
5788
930
UniProt
View information about CD3/CD56/CD16/CD45/CD19 on UniProt.org
Documentation
Technical Data Sheet (pdf)
Certificate of Analysis
Safety Data Sheet
Related Protocols
Cell Surface Immunofluorescence Staining Protocol
Related FAQs
There are no FAQs for this product.
Related Categories
Immunology > Human Immunology > Multicolor Reagents > CD3 /CD56 /CD16 /CD45 /CD19 Cocktail >
Antibodies and More > Flow Cytometry Kits > Antibody Cocktails >
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