Cell types
Fut8+/+ mice
Fut8-/- mice
Leukocytes (µl-1)
6040 ± 381
4730 ± 213
Neutrophils (µl-1)
1080 ± 121
1050 ± 162
Lymphocytes (µl-1)
4850 ± 246
3390 ± 189
Monocytes (µl-1)
30 ± 11
120 ± 37
Eosinophils (µl-1)
70 ± 23
170 ± 43
Basophils (µl-1)
10 ± 3
0±0
Supplemental Figure 1. Total and differential blood leukocyte counts
in Fut8-/- mice compared with respective counts in Fut8+/+ mice
30.6
7.2
5.3
Fut8+/+ SPL
4.3
CD11b
3.8
IgD
Fut8-/- SPL
CD45R
17.8
8.1
3.7
Splenic cell population (%)
CD19
IgM
CD5
Fut8+/+ SPL
Fut8-/- SPL
**
30
20
**
*
10
0 CD45R+CD19+
cell
IgM+IgD+
cell
CD5+
cell
CD11b+
cell
Supplemental Figure 2. Comparison of SPLs compositions between Fut8+/+ and Fut8-/- mice with OVA immunization.
FACS analysis of the proportion of CD19+CD45R+, IgD+IgM+, CD11b+ and CD5+ cells. Data were representative of the
mean ± SD of 5 per genotype (**P < 0.01). Numbers indicate the percentage of the total SPLs within this quadrant,
and 10,000 events were acquired for each analysis.
A
Nucleic acid and amino acid sequence of IgG2a VH
gag guc cag cug cag gag ucu gga gag aca guc agg auc ucc ugc aag gcu ucu ggg uau
E V Q L Q E S G E T V R I S C K A S G Y
acc uuc aca acu gcu gga aug cag ugg gug caa aag aug cca gga aag ggu uug aag ugg
T F T T A G M Q W V Q K M P G K G L K W
auu ggc ugg aua aac acc cac ucu gga gug cca aca uau gca gaa gac uuc aag gga cgg
I G W I N T H S G V P T Y A E D F K G R
uuu gcc uuc ucu uug gaa acc ucu gcc agc acu gca uau uua cag aua agc aac cuc aaa
F A F S L E T S A S T A Y L Q I S N L K
aau gag gac acg gcu acg uau uuc ugu gcg aga uuc ggt agu agc uac uac ugg uac uuc
N E D T A T Y F C A R F G S S Y Y W Y F
gau guc ugg ggc gca ggg acc aug guc acc guc ucc uca
D V W G A G T M V T V S S
B
Nucleic acid and amino acid sequence of IgG2a VL
ccg uuu cag cuc cag cuu ggu ccc ccc ucc gaa cgu gua agc ucc cua aug ugc cag guu
P F Q L Q L G P P S E R V S S L M C Q V
gca gca ucc ucc ucc ucc aca gga ugg aug uug agg gug aag ucu guc cca gac cca cug
A A S S S S T G W M L R V K S V P D P L
cca cug aac cug gca ggg acc cca gau ucu agg uug gau aca aga aug agg agu cug ggu
P L N L A G T P D S R L D T R M R S L G
ggc ugu ccu ggu uuc ugu ugg uuc cag ugc aua cua cca gau gua cug aca cuu uug cug
G C P G F C W F Q C I L P D V L T L L L
gcc cug uau gag aug gug gcc cuc ugc ccc aga gau aca gcu aag gaa gca gga gac ugg
A L Y E M V A L C P R D T A K E A G D W
gug agc aca aug uca
V S T M S
Supplemental Figure 3. No glycosylation sites in VH and VL of IgG2a in the 3-83 cells.
The primers for VH were 5’-SAGGTSCAGCTGMAGGAGTCWGG-3’ and 5’-TGAGGAGACGGTGACCATGGTCCC-3’.
The primers for VL were 5’-CCGTTTCAGCTCCAGCTTGGTCCC-3’ and 5’-GACATTGTGCTCACCCAGTCTCCA-3’.
The cDNA sequence was analyzed by Takara Biotechnology (Dalian) Co. Ltd.