Enclosure 1:
Waste Management Laboratory
Responsibility
All lab users are responsible for the safe and responsible collection of biohazardous and other waste
substances from the laboratory. The assigned Principal Investigator (VM (1)) is responsible for the
upkeep and maintenance or the waste bins, regularly checking to ensure conformance with the
procedures below, or handling spills. The VM is also responsible for proper registration of the
biological waste disposal in a logbook (see form F013; appendix A01).
(1) Abbreviation of “Verantwoordelijk Medewerker” in Dutch, see front page for current VM
Safety precautions
Users are required to wear at least lab coats when handling biological or chemical waste.
Document SCR-G003 contains more detailed instruction about safety precautions for working with
biohazardous samples (ML-1 Site Specifications and Handling Regulations) [1].
The VM is responsible to investigate all incidents and accidents initially, and when deemed necessary
involve the Bio Safety Manager (BVF (2)), who will take appropriate preventative and corrective
measures when required. The accident and incident form (see SCR-F012; appendix A02) is filled out
in all cases that require investigation by the VM, and needs to be followed, filled in and filed.
(2) Abbreviation of “Biologische Veiligheids Functionaris” in Dutch, see front page for current
BVF.
Waste categories
At the Golden Standard Laboratory four classes of waste can be distinguished:
1. Waste with a potential biohazard
Soil samples originating from countries outside the European Union which could contain traces of
hazardous micro-organisms. They can be distinguished from European soil samples, and from all feed
samples, by the differences in the generated identification codes at the Golden standard laboratory.
All containers containing biohazardous soil samples and sub-samples will also at all times be marked
with a biohazard symbol. When not in use these samples are stored separately in dedicated and
clearly marked spaces, and during all sample preparation and analytical steps when the samples can
still be considered hazardous, they are handled separately. Currently it is not foreseen that the
samples themselves will be disposed of as they will be stored as reference samples, but if in the
future they are disposed of, they will be treated as biohazardous waste. The following soil types are
classified as biohazardous:

All non-EU “Golden Standard” soil samples. After registration they will get a unique internal
identification code starting with SCR-SBC00001, SCR-SBC00002, etc. The abbreviation stands
for SoilCaresResearch-SoilBiohazardousCalibration-00001(sample number). They will be
analyzed using the Golden Standard methods and the results will be used to build the
prediction models for non-EU countries.

All other non-EU soil samples. They will get an identification code starting with SCRSBP00001 etc. In this case the abbreviation stands for SoilCaresResearch-
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SoilBiohazardousProject-00001 (sample number). These are project samples which will not
be used to build prediction models, but for other tests and development work.
All consumables used for handling and processing the samples above, including aluminium drying
trays, gloves, overshoes, overalls, and paper towel for wiping and cleaning, as well as dust and waste
resulting from the processing.
2. Hazardous chemical waste
Chemical waste such as hazardous organic and inorganic solutions or solids. Currently this type of
waste is produced in small volumes.
3. Non-hazardous waste
General waste that does not belong to the above mentioned categories, namely waste not classified
as (bio)hazardous, which is removed from the laboratory by personnel.
The following soil and feed types, soil extracts, feed digestions, and ash, are classified as general
waste:

All EU “Golden Standard” soil samples. After registration they will get a unique internal
identification code starting with SCR-SXC00001, etc. which stands for SoilCaresResearchSoil(non-biohazardous)Calibration-00001 (sample number). These samples will be
analyzed by the Golden standard methods and used to build prediction models.

All other EU soil samples will get an identification code starting with SCR-SXP00001 etc.,
where the abbreviation stands for SoilCaresResearch-Soil(non-biohazarous)Project00001 (sample number for soil). These are project samples which will not be used for
database purposes but for other testing and research purposes.
All “Golden Standard” feed samples, irrespective of origin. After registration they will get
a unique internal identification code starting with SCR-FXC00001, etc. which stands for
SoilCaresResearch-Feed(non-biohazardous)Calibration-00001 (sample number). These
samples will be analyzed by the Golden standard methods and used to build prediction
models.


All other feed samples will get an identification code starting with SCR-FXP00001 where
the abbreviation stands for SoilCaresResearch-Feed(non-biohazardous)Project-00001
(sample number for feed). These are project samples which will not be used for database
purposes but for other testing and research purposes.

All soil extracts are either very acidic (pH < 3), or chemically disinfecting (cobalt
concentration ~ 900mg/L; toxicity levels to various organisms summarized in [2]), and
therefore disposed of as non-biohazardous.
All samples which were combusted and ashed are considered non-biohazardous waste.
Containers which had been used during storage and sample preparation of bio-hazardous samples
are treated with 10% bleach solution (completely soaked), rinsed, and then discarded as general
waste.
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Note: All non-biohazardous waste that had been contaminated, or could have been contaminated,
with biohazardous waste, will itself be treated as biohazardous waste.
4. Glass waste
All non-hazardous glassware.
Collection and disposal
The different types of waste that is produced during laboratory activities are collected in separate
and appropriate waste disposal containers. The disposal of waste is outsourced and carried out by
third parties.
For each category a separate container is used:

Biohazardous soil waste, and consumables used for its processing, are collected in 200
liter metal waste drums with lids, supplied by the waste disposal company Dusseldorp
[3]. The lid will be opened prior to each waste disposal and closed afterwards. The drums
may not be filled more than 90% before disposal. When this maximum is reached the
drum must be closed and the waste company contacted to arrange removal of the full
drum, and delivery of an empty drum. An empty drum should always be kept in surplus,
to be available after closure of the full drum. The waste disposal company supplies
proper labelling, and is responsible for transport and destruction of the biohazardous
waste. The biohazard label must be clearly visible while the drums are in the lab, but
removed before being collected by the waste company.

Hazardous chemical waste:

Concentrated acids are collected in 10 liter leak-tight plastic jerry cans kept
in a fume cupboard. During usage a broad plastic funnel is placed in the neck
of the can to prevent spilling. The can remains open until it is filled at 80% of
its maximum capacity. If this maximum is reached the can is closed with a lid
and the waste company contacted to arrange removal of the can. The waste
disposal company will supply proper labelling, and is responsible for
transporting and destruction of the chemical waste.

Sodium dithionite (Na2S2O4) is discarded into 2.5L jerry cans in a fume
cupboard in the lab using a funnel, and when full to 80% maximum capacity
they are emptied in a 60L plastic drum, provided by the waste company
Dusseldorp. When the 60L drum is 80% full the drum is closed and the waste
company contacted to arrange removal of the drum. The waste disposal
company will supply proper labelling, and is responsible for transporting and
destruction of the chemical waste.
General waste is collected in marked garbage bins and bags inside the laboratory. When full these
are disposed into the waste containers placed outside the building by the rental organization
KADANS. These containers are for common usage by all tenants and subdivided into the following
categories:

Containers for general waste labelled “Rest afval”
CSR policy Dutch Sprouts, July 2016
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
A container for paper waste labelled “Papier”

A separate plastic container for glass waste labelled “Glas”
A laboratory waste storage room. Each tenant has a temporary storage compartment for chemical
waste. This compartment will not be used for biohazardous waste.
Biohazardous waste Registration
The biohazardous waste disposal registration form SCR-F013 (Appendix A01) will be used to record
the biohazardous waste disposal from the laboratory. The first column contains the classification
number to be given to each subsequent drum in chronological order (starting with number 001).
Two dates will be registered for each drum, the starting date or first date on which the drum is used,
and the closing date when the drum is 90% full and the lid is closed. All biological waste produced
during the intervening period can be traced back to a specific waste drum. The registration form will
be filled in and signed. After a form is completely filled both the VM and Biological Safety Officer
(BVF) sign off the completed form.
The resulting logbooks will be archived for at least one year. The relevance of archiving data from a
disposed drum after destruction by Dusseldorp becomes relatively low. It is not possible to construct
soil mass balances due to sample loss to extracts, XRF tablets and combustion, moisture loss during
initial drying, and the consumables discarded with the sample material which adds additional mass to
the waste. However the register would give an indication of the chain of custody of a biohazardous
soil sample.
CSR policy Dutch Sprouts, July 2016