Jupiter®
If Jupiter does not provide you with at least
an equivalent separation as compared to
a column of similar phase, particle size
and dimension, send in your comparative
data within 45 days and keep the Jupiter
column for FREE.
Reversed Phase Solutions for the Analysis of Proteins,
Peptides, and Oligonucleotides
TM
The Jupiter line, which includes Jupiter 300 and Jupiter Proteo, offers various reversed phase
solutions for biochromatography. With these two columns, one can identify, purify, and analyze
almost any protein or oligonucleotide.
Jupiter 300 – 300 Å column designed to analyze intact proteins and large biomolecules
Jupiter Proteo – 90 Å column designed to increase the peak capacity and resolution of peptide
maps as well as separate peptides and small oligonucleotides (<30mer)
Material Characteristics
Pore
Size
(Å)
Pore
Volume
(mL/g)
Surface
Area
(m2/g)
Carbon
Load
%
Calculated
Bonded Phase
Coverage
(µmole/m2)
End
Capping
C4
C5
C18
Proteo
Spher. 5, 10, 15
Spher. 5, 10, 15
Spher. 5, 10, 15
Spher. 4, 10
300
300
300
90
—
—
—
—
170
170
170
475
5.0
5.5
13.3
15.0
6.30
5.30
5.50
—
Yes
Yes
Yes
Yes
Jupiter
Particle
Shape/Size
(μm)
HPLC
Packing
Material
Jupiter® 300
For Large Protein, Polypeptide, and Large Oligonucleotide Analysis/
Purification
•Excellent 300 Å column for large proteins, polypeptides (MW>10,000) and oligonucleotides > 30mer
• Stable from pH 1.5 to 10 (3000 hours)
• 5, 10 and 15 μm bonded phases available for convenient scale-up
•Extensive batch traceability data provided with every column
Sharp Peaks and High Yields
Jupiter 300 is designed to analyze and purify intact proteins and macromolecules. Ultra-pure (99.99 % metal-free) silica and dense bonded
phase coverage provide sharp peaks for proteins. Dense bonded phase coverage combined with a relatively high silica surface area
yields a high capacity sorbent for high loadability. Jupiter 300 is an excellent column for biological purification.
Chromatographic Comparisons of 5 μm, C4, 300 Å 250 x 4.6 mm Columns*,**
Peptide Hormones**
Jupiter® 300
Vydac®
2
1000
1
1
800
500
4
600
4
2
400
3
3
300
12
14 min
App ID 5367
200
200
30
35 min
App ID 5368
App ID 5365
400
100
0
0
2
4
6
8
YMC-Pack™
10
12
2
14 min
6
8
10
SynChropak®
1
1
500
4
2
400
3
400
4
2
3
300
4
App ID 5366
300
200
200
100
100
0
0
2
4
6
8
10
12
14 min
5
10
15
*This comparative data may not be representative for all applications.
**Better results might be achieved by columns of different pore sizes.
Jupiter is a registered trademark of Phenomenex, Inc. Jupiter column manufactured by Phenomenex.
Phenomenex is a registered trademark of Phenomenex, Inc. Vydac is a registered trademark of Alltech
Associates, Inc. Vydac column purchased from Grace Vydac. Phenomenex is not associated with Grace
Vydac.
Phenomenex
20
25
Conditions for all columns:
Dimensions:
250 x 4.6 mm
Mobile Phase: A: 0.1 % TFA in Water
B: 0.1 % TFA in Acetonitrile/Water
(90:10)
Gradient:
a) A/B (90:10) to A/B (74:26) in 8
min (2 % B/min)
b) A/B (74:26) to A/B (70:30) in 6
min (0.57 % B/min)
Flow Rate:
1. 0 mL/min
Detection:
UV @ 214 nm
Sample:
1. [Arg8]-Vasotocin
(Cys-Tyr-Ile-Gln-Asn Cys-Pro-Arg-Gly-NH2)
2. [Arg8]-Vasopressin
(Cys-Tyr-Phe-Gln-Asn Cys-Pro-Arg-Gly-NH2)
3. Isotocin
(Cys-Tyr-Ile-Ser-Asn Cys-Pro-Ile-Gly-NH2)
4. Oxytocin
(Cys-Tyr-Ile-Gln-Asn Cys-Pro-Leu-Gly-NH2)
Zorbax is a registered trademark of Agilent Technologies. Zorbax column purchased from MAC-MOD.
Phenomenex is not associated with Agilent Technologies or MAC-MOD. YMC is a registered trademark
of YMC Co., Ltd. YMC column purchased from YMC. Phenomenex is not associated with YMC Co., Ltd.
SynChropak is a registered trademark of Eprogen, Inc. SynChropak column purchased from Eichrom
Technologies, Inc. Phenomenex is not associated with Eichrom Technologies, Inc. nor Eprogen, Inc.
143
Jupiter®
Jupiter 300 (cont'd)
TM
pH 1.5 - 10 Stability
High bonded phase surface coverage and uniformity is achieved
using our bonding process. Dense bonding provides pH stability from
pH 1.5 to 10 for more than 3000 hours of use. This exceptional pH
stability allows for easy column cleaning as well as the use of mobile
phases that maintain protein biologic activity.
Jupiter
HPLC
Low TFA Conditions
TFA can actually be a drawback when electrospray (ESI) interfaces
are used to introduce samples into the mass spectrometer. At
typical working concentrations (0.1 %), TFA has been shown
to greatly reduce ion generation in ESI-MS, presumably due to
a combination of its ion-pairing capacity as well as its role as
a competitive ion. The use of TFA does provide excellent peak
shapes, but at the cost of reduced sensitivity in LC/ESI-MS.
Jupiter, however, provides excellent peak shapes for proteins even
at reduced concentrations (0.01 % TFA) without compromising
peak symmetry.
Rapid LC/MS Analysis
•Reduce analysis time, solvent usage and
column equilibration time by as much as 70 %
for some applications
• Increase sample throughput by as much as 70 %
while maintaining excellent sensitivity, resolution
and the accuracy of your Mass Spec data
Below are MS results for protein and peptide samples comparing
Jupiter 300 5 μm 300 Å LC/MS 50 x 2.0 mm columns to more
conventional Jupiter 300 5 μm 300 Å 250 x 2.0 mm columns.
LC/MS (C18)
Sample:
MS:
Scan Range:
Myoglobin Tryptic Digest
HP5989
130-1000 in 1 sec
70 % reduction in
analysis time
Total Ion Chromatograms
App ID 14184
App ID 5375
A
Proteins Run Under 0.1 % TFA
Column:
Dimensions:
Part No.:
Flow Rate: Mobile Phase:
Gradient:
Detection:
Temperature:
Sample:
Jupiter 300 5 μm C4 300 Å
250 x 4.6 mm
00G-4167-E0
1 mL/min
A: 0.1 % TFA in Water
B: 0.1 % TFA in Acetonitrile
A/B (75:25) to A/B (5:95) in 20 min
UV @ 214 nm
35 °C
1. Insulin
2. Trypsinogen
3. Lactalbumin
4. Myoglobin
5. Carbonic anhydrase
250 x 2.0 mm
B
50 x 2.0 mm
10
20
30
40
Mass Spectra of Selected Myoglobin Peptide
App ID 5376
50
min
Near identical
results
A
App ID 14186
250 x 2.0 mm
144
Proteins Run Under 0.01 % TFA
Column:
Dimensions:
Part No.:
Flow Rate: Mobile Phase:
Gradient:
Detection:
Temperature:
Sample:
Jupiter 300 5 μm C4 300 Å
250 x 4.6 mm
00G-4167-E0
1 mL/min
A: 0.01 % TFA in Water
B: 0.01 % TFA in Acetonitrile
A/B (75:25) to A/B (5:95) in 20 min
UV @ 214 nm
35 °C
1. Insulin
2. Trypsinogen
3. Lactalbumin
4. Myoglobin
5. Carbonic anhydrase
B
50 x 2.0 mm
200
300
400
500
600
700
800
A
Column:
Dimensions:
Part No.:
Flow Rate: Mobile Phase:
Jupiter 300 5 μm C18 300 Å
250 x 2.0 mm
00G-4053-B0
0.2 mL/min
A: 0.1 % TFA in Water
B: 0.1 % TFA in Acetonitrile
A/B (95:5) to A/B (5:95) in 60 min
B
Column:
Dimensions:
Part No.:
Flow Rate:
Mobile Phase:
Jupiter 300 5 μm C18 300 Å
50 x 2.0 mm
00B-4053-B0
0.2 mL/min
A: 0.1 % TFA in Water
B: 0.1 % TFA in Acetonitrile
A/B (95:5) to A/B (5:95) in 45 min
900 m/z
Phenomenex
Jupiter®
Jupiter 300 (cont'd)
Large Oligonucleotides - Separation and Purification
• Reduce solvent usage and column cost
• Maintain excellent resolution by utilizing
steeper gradients
• Excellent as method development or
screening columns
Jupiter 300 5 μm C4 300 Å
1.0 mL/min
UV @ 220 nm
5 µL
1. Alkaline phosphatase
2. Cyanocobalamin
3. RNase
4. Insulin
5. Transferrin
6. Trypsin Inhibitor
A
4
1
5
0
6
250 x 4.6 mm
2
34
Rt=5 min
5
2
200
1
B
Jupiter 300 5 μm C18 300 Å
150 x 4.6 mm
00F-4053-E0
A) 100 mM TEAA in Water, pH 7.0
B) 100 mM TEAA in Acetonitrile, pH 7.0
A/B (96:4) to A/B (85:15) in 30 min
1 mL/min
50 °C
UV @ 260 nm
1. Failed sequence contaminant
2. 40mer Oligonucleotide
400
2
3
Rt=15 min
Column: Dimension:
Part No.:
Mobile Phase:
Gradient: Flow Rate:
Temperature:
Detection:
Sample: 600
70 % reduction
in analysis time and
savings
0
App ID 14627
App ID 5378
Column:
Flow Rate:
Detection:
Injection:
Sample:
40mer Oligonucleotide Separation
Jupiter
Proteins on Jupiter C4
TM
HPLC
The mechanism of retention and separation of large molecules
like polypeptides by reversed-phase HPLC is primarily adsorption/
desorption, although partitioning appears to play a minor role.
When using shorter columns (50 mm), reduced analysis times
require steeper gradients to reach the same critical organic
concentration. This is necessary for desorption of polypeptides
in a shorter time compared to those used on longer length
columns.
App ID 14625
Jupiter 300 provides excellent separation for oligonucleotides in
excess of 30 residues. Such separation allows one to quickly purify
large oligonucleotides from failed sequence contaminants and
other oligonucleotides. The following applications demonstrate the
resolving power of Jupiter 300 in separating large oligonucleotides
from contaminants generated during synthesis.
Cut Analysis Time by as Much as 70 %
6
1
10
20
30 min
60mer Oligonucleotide Separation
Column: Dimension:
Part No.:
Mobile Phase:
Gradient: Flow Rate:
Temperature:
Detection:
Sample: Jupiter 300 5 μm C18 300 Å
150 x 4.6 mm
00F-4053-E0
A) 100 mM TEAA in Water, pH 7.0
B) 100 mM TEAA in Acetonitrile, pH 7.0
A/B (96:4) to A/B (85:15) in 30 min
1 mL/min
50 °C
UV @ 260 nm
1. Failed sequence contaminant
2. 60mer Oligonucleotide
50 x 4.6 mm
600
2.5
A
B
Phenomenex
5.0
7.5
10.0
12.5
15.0
2
17.5 min
Dimensions:
Part No.:
Mobile Phase:
Gradient:
250 x 4.6 mm
00G-4167-E0
A: 0.1 % TFA in Water
B: 0.1 % TFA in Acetonitrile
a) A/B (95:5) to A/B (74:26) in 7 min (3 % B/min)
b) A/B (74:26) to A/B (66:34) in 3 min (2.67 % B/min)
c) A/B (66:34) to A/B (46:54) in 10 min (2 % B/min)
Dimensions:
Part No.:
Mobile Phase:
Gradient:
50 x 4.6 mm
00B-4167-E0
A: 0.1 % TFA in Water
B: 0.1 % TFA in Acetonitrile
a) A/B (100:0) to A/B (80:20) in 1 min (20 % B/min)
b) A/B (80:20) to A/B (65:35) in 1.5 min (10 % B/min)
c) A/B (65:35) to A/B (53.5:46.5) in 1.5 min (7.67 % B/min)
d) A/B (53.5:46.5) to A/B (53.5:46.5) in 2 min (constant B)
400
200
1
0
0
10
20
30 min
145
Jupiter®
Jupiter 300 (cont'd)
TM
Quality Proven
Jupiter
HPLC
Quality is carefully maintained and traceability is assured
throughout the manufacturing process. Each column is shipped
with extensive batch traceability data verifying batch quality, as
well as with its own individual test chromatogram.
A Materials Validation Document (MVD) accompanies every Jupiter
column. Each certificate contains information on the rigorous
testing procedures performed on each batch of Jupiter material
to ensure column-to-column and batch-to-batch reproducibility.
The information below is provided with each column.
• Particle Analysis
- Particle Size
- Pore Diameter
- Particle Size Distribution
- Surface Area
• Total Metal Content
• Bonded Phase Coverage
- Total Carbon
- Surface Coverage
• Diagnostic Chromatography Test
- Inertness
- Metal Sensitivity
- Hydrophobic Index
• Performance Chromatography Test
- Protein Standards
- Longevity Tests (pH Stability)
- Scanning Electron Microscopy
(measures surface smoothness
and particle shape)
Reproducibility Assured
To demonstrate the reproducibility of the results obtained on
different batches of Jupiter material, Cytochrome c variants were
used for their similar chemical structures. Consistency of resolution
factors, capacity factors, retention times and peak asymmetries
for these difficult-to-resolve variants were put to the test for you
to judge.
Batch-to-Batch Reproducibility
Column:
Dimensions:
Part No.:
Mobile Phase:
Gradient:
Flow Rate:
Detection:
Sample:
Jupiter 300 5 μm C18, 300 Å
250 x 4.6 mm
00G-4053-E0
A: 0.1 % TFA in Water
B: 0.1 % TFA in Acetonitrile
A/B (75:25) to A/B (45:55) in 15 min (2 % B/min)
1.0 mL/min
UV @ 220 nm
1. Equine Cytochrome C
2. Bovine Cytochrome C
3. Canine Cytochrome C
1 2
3
Batch
5243-4
Batch
5243-1
1 2
3
Batch
5243-5
Batch
5243-2
3
1 2
Batch
5243-6
Batch
5243-3
0
App ID 5357
146
2
4
6
8
10
12
14 min
App ID 5358
Phenomenex
Jupiter®
RNA Fragments
Column:
Dimensions: Part No.:
Mobile Phase: Gradient:
Flow Rate:
Detection: Sample:
App ID 14909
App ID 5359
Jupiter 300 (cont'd)
Jupiter 300 5 μm C4 300 Å
250 x 4.6 mm
00G-4167-E0
A: 0.1 M K3PO4 in 0.75 % Isopropanol, pH 6.8
B: 1.0 M (NH4) SO4 + 0.1 M K3PO4 in 0.75 %
Isopropanol, pH 6.8
100-30 % B for 20 minutes,
30-0 % B for 10 minutes,
0 % B for 40 minutes
1.0 mL/min
UV @ 260 nm
tRNA Fragments
Reduced Dog IgG
Column:
Dimensions: Part No.:
Mobile Phase: Gradient:
Flow Rate:
Detection: Sample:
TM
Jupiter 300 5 μm C4 300 Å
150 x 2.0 mm
00F-4167-B0
A: 0.1 % TFA in Water
B: 0.085 % TFA in Water/ Acetonitrile/IPA (5:75:20)
A/B: 80:20 to A/B: 5:95 in 20 minutes
0.25 mL/min
UV @ 220 nm
IgG Dog Reduced
HPLC
Jupiter
20
40
60
80
Proteins
Column:
Dimensions:
Part No.:
Mobile Phase:
Gradient:
Flow Rate:
Detection:
Sample:
Jupiter 300 5 μm C4 300 Å
50 x 4.6 mm
00B-4167-E0
A: 0.1 % TFA in Water
B: 0.1 % TFA in Acetonitrile
a) A/B (100:0) to A/B (80:20) in 1 min (20 % B/min)
b) A/B (80:20) to A/B (65:35) in 1.5 min (10 % B/min)
c) A/B (65:35) to A/B (53.5:46.5) in 1.5 min (7.67 % B/min)
d) A/B (53.5:46.5) to A/B (53.5:46.5) for 2 min (constant B)
1.0 mL/min
UV @ 220 nm
1. Alkaline Phosphatase
2. Cyanocobalamin
3. RNase
4. Insulin
5. Transferrin
6. Trypsin Inhibitor
300
3
250
MIN
Insulin Genetic Variants
Column:
Dimensions:
Part No.:
Mobile Phase:
Gradient:
Flow Rate:
Detection:
Sample:
Jupiter 300 5 μm C18 300 Å
250 x 4.6 mm
00G-4053-E0
A: 0.1 % TFA in Water
B: 0.1 % TFA in Acetonitrile
A/B (70:30) to A/B (68:32) in 20 min
1.0 mL/min
UV @ 210 nm
1. Bovine Insulin
2. Human Insulin
3. Porcine Insulin
800
4
200
5
2
600
6
1
3
1
400
100
200
50
0
0
0
Phenomenex
1000
350
150
mAU
2
mAU
110 min
App ID 5372
App ID 5370
0
1
2
3
4
5
min
2.5
5.0
7.5
10.0
12.5
15.0
17.5 min
147
Jupiter®
Jupiter® Proteo
TM
Increase Peak Capacity by 40-50 %
Jupiter Proteo is designed to selectively separate and to
optimize information on peptide fragments obtained in a protein
digest, which is a standard method of protein characterization.
In Figure 1, a Jupiter Proteo column offers better selectivity
and resolving power in every aspect compared with several
commercial 300 Å, silica-based C18 reversed-phase columns
traditionally employed for the analysis of protein digests and
synthetic peptides. Further, special bonding technology permits
the use of less trifluoracetic acid or other ion-pair agents without
compromising peak symmetry and efficiency.
• For protein digest, peptide mapping,
synthetic peptides, and oligonucleotides < 30mer
•Excellent 90 Å pore column for separating
polypeptide fragments
• Stable from pH 1.5 to 10
•Excellent peak symmetry in other modifiers
besides TFA
Jupiter
HPLC
• 4 and 10 μm particle available from capillary to
preparative columns
•Extensive column-to-column and batch-to-batch
reproducibility data provided
Figure 1: Myoglobin Tryptic Digest Comparisons
App ID 14408
App ID 14411
Jupiter 4 μm
90 Å
Proteo
®
1
2
118 72
3
71
4
49
Avg.
78
Zorbax 5 μm
SB C18 300 Å
1
54
2
51
3
50
4
35
Avg.
48
peaks
Vydac® 5 μm
MS C18 300 Å
1
62
2
59
3
59
4
33
Avg.
53
peaks
®
78
peaks
48
Myoglobin Tryptic Digest
Conditions for all columns:
Dimensions:
250 x 4.6 mm
Mobile Phase: A) 0.012 % TFA in Water
B) 0.01 % TFA in Acetonitrile
Gradient:
A/B (95:5) for 5 min, then to
A/B (60:40) in 55 minutes
Flow Rate:
1 mL/min
Temperature: 22 °C
Detection:
UV @ 210 nm
Sample:
Myoglobin Tryptic Digest
53
App ID 14412
Determining peak counts - The large number of peaks in a given tryptic digest makes
counting peaks visually both inaccurate and subjective. For a more accurate approach,
peak counting was performed using Agilent Technologies (HP) ChemStation™ software.
Four different integration parameters at different sensitivity settings were used in
calculating the number of peaks and an average. The parameters changed within each
method were: minimum peak area, minimum peak height, peak width, and threshold.
The table below describes the parameters used for each calculation.
MethodThreshold
148
Peak Width
Min Area
1
1.0
0.1
10.0
Min Height
20.0
2
2.0
0.2
10.0
20.0
3
3.0
0.3
20.0
10.0
4
3.0
0.3
20.0
50.0
Phenomenex
Jupiter®
Jupiter Proteo (cont'd)
Figure 2: Comparison of Methylene Selectivity
TM
App ID 14426
Selectivity to Improve Resolution
Zorbax® 5 μm
SB-C18 300 Å
App ID 14428
App ID 14429
Vydac® 5 μm
TP54 300 Å
Easily Monitor Protein Degradation
The shelf life of a protein product is a major concern in the
biotechnology industry as it may be subject to inactivation from
deamination or oxidation. Oxidation is commonly seen with
methionine due to its readily oxidized sulfur group. A tryptic
digest of ß-Lactoglobulin reveals early eluting peaks of a more
polar peak representing the oxidation product. Deamidation of
asparagine forms aspartic acid which is less polar and elutes
slightly later in a chromatographic profile. Monitoring protein
oxidation and deamidation peaks within the jungle of a tryptic
digest can be difficult without adequate resolution, thus Jupiter
Proteo was engineered with one goal: high resolution.
Oxidation of β-Lactoglobulin
Methylene Selectivity
Conditions for all columns:
Dimensions:250 x 4.6 mm
Mobile Phase: A) 0.1 % TFA
B) 0.085 % TFA in Acetonitrile
Gradient:
A/B (95:5) to
A/B (55:45) in 20 minutes
Flow Rate:
1 mL/min
Temperature: 22 °C
Detection:
UV @ 214 nm
Sample:
1. NH2-Arg-Gly-Gly-Ala-Gly-Gly-Leu-Gly-Leu-Gly-Lys-Amide
2. Ac-Arg-Gly-Gly-Gly-Gly-Gly-Leu-Gly-Leu-Gly-Lys-Amide
3. Ac-Arg-Gly-Ala-Gly-Gly-Gly-Leu-Gly-Leu-Gly-Lys-Amide
4. Ac-Arg-Gly-Val-Gly-Gly-Gly-Leu-Gly-Leu-Gly-Lys-Amide
5. Ac-Arg-Gly-Val-Val-Gly-Gly-Leu-Gly-Leu-Gly-Lys-Amide
Columns: Dimensions:
Part No.:
Mobile Phase:
Gradient:
Flow Rate:
Temperature:
Detection:
Sample:
Jupiter
Vydac® 5 μm
MS54 300 Å
Jupiter Proteo can be used to resolve peptides of MW
≤10,000 Da, and often is able to separate peptides
of only 1-2 amino acid difference. Figure 2 compares
the resolution of five peptide standards with amino
acid sequences that differ in hydrophobicity by one
methyl group each. Comparing the differences with
respect to efficiency, selectivity, and resolution allows
us to monitor the overall column performance; Jupiter
Proteo fully resolves each peptide. Sharper peaks
with greater resolution are obtained with Jupiter
Proteo as compared to the competition.
HPLC
App ID 14427
Jupiter® 4 μm
Proteo 90 Å
Jupiter 4 μm Proteo 90 Å
250 x 4.6 mm
00G-4396-E0
A) 0.012 % TFA in Water
B) 0.01 % TFA in Acetonitrile
A/B (95:5) for 5 min, then to A/B (60:40) in 55 minutes
1 mL/min
22 °C
UV @ 210 nm
Top Chromatogram – β-Lactoglobulin tryptic digest Bottom Chromatogram – Oxidized β-Lactoglobulin tryptic digest
App ID 14394
Control
Oxidation
Product
App ID 14395
Phenomenex
149
Jupiter®
Capillary Columns
Jupiter Proteo (cont'd)
TM
• High efficiency 0.30 and 0.50 mm ID
glass-lined columns
Oligonucleotides-Purification and
Analysis on Jupiter Proteo
12-18mer Poly-dT Oligonucleotide Mix
App ID 14620
Jupiter
HPLC
Molecular biology techniques and high-throughput assays require
synthesized oligonucleotides that are highly purified. Jupiter Proteo
provides excellent separation of oligonucleotides such that single
base differences can be resolved. This excellent resolution of
oligonucleotides can be used to assess purity or can be used to
purify oligonucleotides from other failed sequence contaminants
generated during oligonucleotide synthesis.
Column: Dimension:
Part No.:
Mobile Phase:
Gradient: Flow Rate:
Temperature:
Detection:
14620
15
3
4
5
6
mAU
7
5
0
App ID 14621
0
5
10
15
20 min
20mer Synthetic Oligonucleotide Purification
Column: Dimension:
Part No.:
Mobile Phase:
Gradient: Flow Rate:
Temperature:
Detection:
• Consistent performance under LC/MS
compatible and universal gradient conditions
• 1/8 inch (3 mm) OD tube for robust columns
and durability
App ID 14336
2
1
• Packed with identical Jupiter materials as
analytical columns for easy method transfer
Capillary-LC columns packed with Jupiter 300 and Jupiter Proteo
materials provide the same characteristics as their respective
analytical sized columns, but with added sensitivity for microliter
sample volumes. Our high purity silica combined with proprietary
bonding techniques produce dense bonded phase coverage
to shield against silanol interactions. With little secondary
chromatographic effects Jupiter capillary-LC columns yield terrific
peak shape and are compatible with reduced TFA concentration
and other mass spec compatible mobile phase modifiers.
Jupiter 4 μm Proteo 90 Å
50 x 4.6 mm
00B-4396-E0
A) 0.1 M TEAA pH 7.0
B) Acetonitrile
A/B (97: 3) to A/B (85:15) in 20 min
1 mL/min
60 °C
UV @ 260 nm 10
• High sensitivity for low volume and
mass limited samples
Jupiter 4 μm Proteo 90 Å
50 x 4.6 mm
00B-4396-E0
A) 0.1 M TEAA pH 7.0
B) Acetonitrile
A/B (97: 3) to A/B (85:15) in 20 min
1 mL/min
60 °C
UV @ 260 nm α-Chymotrypsin Tryptic Digest on Jupiter Proteo
Column:
Dimensions:
Part No.:
Mobile Phase:
Gradient:
Flow Rate:
Temperature:
Detection:
Sample:
Jupiter 4 μm Proteo 90 Å
150 x 0.5 mm
00F-4396-AF
A: 0.01 % TFA in Water
B: 0.008 % TFA in Acetonitrile
A/B (95:5) for 5 min then
A/B (55:45) in 45 min
Switch to A/B (95:5) in 1 sec
then hold for 10 min
15 µL/min
40 °C
UV @ 210 nm
α - Chymotrypsin tryptic digest
14621
1
80
60
0
10
20
30
40 min
40
mAU
20
0
150
4
6
8
10
12
14 min
Phenomenex
Jupiter®
Prep/Process
Columns
• 10 and 15 μm material with identical technology
and base silica as 5 μm analytical products
• High mechanical strength with improved
surface smoothness and particle sphericity
• High loadability with uniform, consistent
pore structure
• High pH stability for easier column cleaning
Jupiter materials have extremely low particle densities; meaning
less material is required to pack a given volume. For large-scale
preparative system this can have an enormous impact on longterm operating costs.
Excellent Lifetime
The excellent silica and bonding technology at the heart of Jupiter
provides excellent lifetimes for improved overall economy. Jupiter is
stable over a wide pH range (1.5 to 10) allowing for easier column
cleaning for added column lifetime.
Since many biochromatographers work with a variety of proteins
and peptides, Phenomenex has made column selection easier
with the Jupiter Method Development Kit. The kit provides you
with one each 250 x 4.6 mm C4, C5 and C18 columns so you
may screen your sample on several phases and choose the
column with the best selectivity and resolution.
Method reproducibility is easily confirmed with the Jupiter
Method Validation Kit. These kits provide three columns from
three different material batches. Kits are available with 5 μm
C18 or C4 materials in a 250 x 4.6 mm column.
Which Jupiter® Phase is right for your application
Phase
Application
C4
For proteins and polypeptides >10,000 MW
For highly hydrophobic proteins
C5
For proteins and polypeptides >10,000 MW
For highly hydrophobic proteins
More retentive than C4, offering slightly alternative selectivity
C18
For proteins and polypeptides >10,000 MW
For small hydrophilic proteins
For separation of oligonucleotides >30mer
Proteo
(C12)
For peptides <10,000 MW, Insulin
For peptide mapping & protein digests
For separation of oligonucleotides <30mer
Jupiter
Less Material Required
TM
HPLC
Jupiter uses identical bonding and base silica technology in both
analytical and preparative materials. Accordingly, Jupiter 300 5
μm C18 material used in the analytical separation is available in a
10 μm or 15 μm version so you can easily scale-up with minimal
changes to the separation.
Method Validation
and Method
Development Kits
ORDERING INFORMATION
Method Development Kits
Part No. DescriptionUnit
Price
I Jupiter Bioseparations
I kit
I
KH0-3983
Method Development Kit
Contains one each (250 x 4.6 mm)
5 μm C4, C5, & C18 columns
KHO-7274
Jupiter Bioseparations kit
Method Development Kit
Contains one each (250 x 4.6 mm)
5 μm C5, C18, & 4 μm Proteo
Method Validation Kits
Part No. DescriptionUnit Price
I Jupiter 5 μm C4 Method Validation Kit I3/pk
I
KH0-4155
250 x 4.6 mm
KH0-4154 Jupiter 5 μm C5 Method Validation Kit 3/pk
250 x 4.6 mm
KH0-4153 Jupiter 5 μm C18 Method Validation Kit 3/pk
250 x 4.6 mm
See pp. 344-345 for Column Heaters.
Phenomenex
151
Jupiter®
If Jupiter does not provide you with at least
an equivalent separation as compared to
a column of similar phase, particle size
and dimension, send in your comparative
data within 45 days and keep the Jupiter
column for FREE.
ORDERING INFORMATION
4 μm & 5 μm Capillary Columns (mm)
TM
50 x 0.30
150 x 0.30
250 x 0.30
50 x 0.50
150 x 0.50
Phases
I 00B-4167-AC I 00F-4167-AC I 00G-4167-AC I 00B-4167-AF I 00F-4167-AF I
5μm C4 300 Å
5μm C18 300 Å 00B-4053-AC 00F-4053-AC 00G-4053-AC 00B-4053-AF 00F-4053-AF
4μm Proteo 90 Å 00B-4396-AC 00F-4396-AC 00G-4396-AC 00B-4396-AF 00F-4396-AF
250 x 0.50
00G-4167-AF
00G-4053-AF
00G-4396-AF
HPLC
SecurityGuard™ Analytical Cartridges require universal holder Part No.: KJO-4282
4 μm & 5 μm Microbore and Minibore Columns (mm)
50 x 1.0 150 x 1.0 250 x 1.0
Phases
5 μm C4 300 Å I 00B-4167-A0 I 00F-4167-A0 I 00G-4167-A0 I
5 μm C5 300 Å 00B-4052-A0 00F-4052-A0 00G-4052-A0
5 μm C18 300 Å 00B-4053-A0 00F-4053-A0 00G-4053-A0
4 μm Proteo 90 Å 00B-4396-A0 00F-4396-A0 00G-4396-A0
50 x 2.0
00B-4167-B0 I
00B-4052-B0
00B-4053-B0
00B-4396-B0
SecurityGuard™ Cartridges
150 x 2.0 250 x 2.0 4 x 2.0 mm*
/10pk
00F-4167-B0 I 00G-4167-B0 AJ0-4329
00F-4052-B0 00G-4052-B0 AJ0-4326
00F-4053-B0 00G-4053-B0 AJ0-4320
00F-4396-B0 00G-4396-B0 AJO-6073
Jupiter
for ID: 2.0-3.0 mm
4 μm & 5 μm Analytical and Preparative Columns (mm)
Phases
5 μm C4 300 Å I
5 μm C5 300 Å
5 μm C18 300 Å
4 μm Proteo 90 Å
30 x 4.6
50 x 4.6
00A-4167-E0 I
00A-4052-E0
00A-4053-E0
00A-4396-E0
00B-4167-E0 I
00B-4052-E0
00B-4053-E0
00B-4396-E0
SecurityGuard™ Cartridges
150 x 4.6 250 x 4.6 250 x 10 250 x 15 250 x 21.2
00F-4167-E0 I 00G-4167-E0 I 00G-4167-N0 I 00G-4167-AK I 00G-4167-P0
00F-4052-E0 00G-4052-E0 00G-4052-N0
— 00G-4052-P0
00F-4053-E0 00G-4053-E0 00G-4053-N0 00G-4053-AK 00G-4053-P0
00F-4396-E0 00G-4396-E0 00G-4396-N0­
— 00G-4396-P0
4 x 3.0 mm*10 x 10 mm‡
/10pk
/3pk
AJO-4330 I AJO-7225
AJO-4327 AJO-7371
AJO-4321 AJO-7224
AJO-6074 AJO-7275
for ID: 3.2-8.0 mm 9-16 mm
10 μm Analytical and Preparative Columns (mm)
SecurityGuard™ Cartridges
250 x 4.6 250 x 10 250 x 15 250 x 21.2 250 x 30 250 x 50 4 x 3.0 mm* 10 x 10 mm‡15 x 21.2 mm**
Phases
/10pk
/3pk
/ea
I 00G-4168-E0 I 00G-4168-N0 I 00G-4168-AK I 00G-4168-P0 I00G-4168-U0 I00G-4168-V0 AJO-4330 I AJO-7225 I AJ0-7231
C4 300 Å
C5 300 Å 00G-4054-E0 00G-4054-N0
— 00G-4054-P0
— 00G-4054-V0 AJO-4327 AJO-7371
—
C18 300 Å 00G-4055-E0 00G-4055-N0
— 00G-4055-P000G-4055-U0 00G-4055-V0 AJO-4321 AJO-7224 AJ0-7230
Proteo 90 Å 00G-4397-E0 00G-4397-N0 00G-4397-AK 00G-4397-P000G-4397-U0 00G-4397-V0 AJO-6074 AJO-7275 AJ0-7842
for ID: 3.2-8.0 mm
15 μm Analytical and Preparative Columns (mm)
9-16 mm
18-30 mm
SecurityGuard™ Cartridges
250 x 4.6 250 x 10 250 x 15 250 x 21.2 250 x 30 250 x 50 4 x 3.0 mm* 10 x 10 mm‡15 x 21.2 mm**
Phases
/10pk
/3pk
/ea
I 00G-4169-E0 I 00G-4169-N0 I 00G-4169-AK I 00G-4169-P0 I 00G-4169-U0 I 00G-4169-V0 AJO-4330 I AJO-7225 I AJ0-7231
C4 300 Å
C5 300 Å 00G-4056-E0 00G-4056-N0
— 00G-4056-P0
— 00G-4056-V0 AJO-4327 AJO-7371
—
C18 300 Å 00G-4057-E0 00G-4057-N0 00G-4057-AK 00G-4057-P0 00G-4057-U0 00G-4057-V0 AJO-4321 AJO-7224 AJ0-7230
Other Dimensions available upon request.
for ID: 3.2-8.0 mm
Bulk Material
ORDERING INFORMATION
10 μm Bulk Packings
100 g
Phase
I 04G-4168 I
C4 300 Å
C5 300 Å 04G-4054
C18 300 Å 04G-4055
Proteo 90 Å 04G-4397
1 kg
04K-4168 I
04K-4054
04K-4055
04K-4397
15 μm Bulk Packings
100 g
1 kg
Phases
I 04G-4169 I 04K-4169 I
C4 300 Å
C5 300 Å 04G-4056 04K-4056
C18 300 Å 04G-4057 04K-4057
5 kg
04L-4168 I
04L-4054
04L-4055
04L-4397
5 kg
10 kg
04M-4168 I
04M-4054
04M-4055
04M-4397
10 kg
50 kg
04N-4168 I
04N-4054
04N-4055
04N-4397
50 kg
04L-4169 I 04M-4169 I 04N-4169 I
04L-4056 04M-4056 04N-4056
04L-4057 04M-4057 04N-4057
See p. 282 for Fused Silica Capillary Adapter and
Capillary Guard Columns.
Effectively desalt acidic, basic, and neutral peptides with
strata-X. See p. 9 for more information.
152
9-16 mm
18-30 mm
*SecurityGuard™ Analytical Cartridges require holder, Part No.: KJ0-4282
‡
Semi-prep SecurityGuard™ Cartridges require holder, Part No.: AJ0-7220
**PREP SecurityGuard™ Cartridges require holder, Part No.: AJ0-8223
100 kg
04P-4168
04P-4054
04P-4055
04P-4397
100 kg
04P-4169
04P-4056
04P-4057
See p. 345 for Column Chiller/Heater System
(8-70 °C).
See p. 219 for SecurityGuard Cartridge
Holders and Cartridges.
Phenomenex