Product Information
Revised: 11–Aug–2006
Monoclonal Antibodies Specific for Oxidative Phosphorylation
Quick Facts
Storage upon receipt:
All products, except A21296, A21297
• ≤–20°C
• Desiccate
Products A21296, A21297
• 2–6°C
• Protect from light
Introduction
Oxidative phosphorylation (OxPhos) occurs in mitochondria
and, in mammals, is catalyzed by five membrane-bound
protein complexes, namely NADH-ubiquinol oxidoreductase
(Complex I), succinate-ubiquinol oxidoreductase (Complex II),
ubiquinol–cytochrome c oxidoreductase (Complex III), cytochrome c oxidase (COX) (Complex IV), and ATP synthase
(Complex V) (Figure 1). The complexes are composed of multiple subunits, some of which are encoded in the mitochondria
and others in the nucleus. Assembly of each complex involves a
coordinated association of prosthetic groups with the polypeptides. This complicated process requires assembly factors specific
for each complex. Defects in assembly of one or more of these
complexes contribute to several described mitochondrial diseases
and possibly Alzheimer’s and Parkinson’s diseases.1–6
Molecular Probes offers a range of subunit-specific antibodies
for the study of mitochondrial function (Tables 1–3 and Figure 1),
including monoclonal antibodies specific for subunits of human
COX (Complex IV) and for representative subunits of human
Complexes I, II, III, and V. These monoclonal antibodies serve
as important tools for investigating mitochondrial biogenesis and
OxPhos-related diseases. Cell lines can be screened for subunit
expression levels for each of the OxPhos complexes by simple
Western blotting.7,8 These results can be combined with native
gel electrophoresis or sucrose gradient centrifugation to gather
additional information regarding the assembly state of the OxPhos
complexes.9 Many of our antibodies against OxPhos subunits
may also be used for immunocytochemical analysis. Image
analysis of an antibody’s staining pattern can reveal the relative
expression and localization of a subunit. This approach has been
particularly useful for studying OxPhos subunit expression in
diseased muscle fibers 10 and for screening Complex IV–deficient
patients.11
For detection of these monoclonal antibodies, Molecular
Probes offers anti–mouse IgG secondary antibodies labeled with
fluorophores, biotin, enzymes such as horseradish peroxidase or
alkaline phosphatase, NANOGOLD ® or Alexa Fluor ® FluoroNanogold™ 1.4 nm gold clusters, or Captivate™ ferrofluid. For
labeling combinations of mitochondrial targets, the antibodies
in this group can be complexed with the reagents in our Zenon®
Mouse IgG Labeling Kits or isotype-specific secondary antibodies. See our website (probes.invitrogen.com) for more information about these products.
Figure 1. The mammalian oxidative phosphorylation system, and specific monoclonal antibodies available from Molecular Probes.
MP 06401
Monoclonal Antibodies Specific for Oxidative Phosphorylation
Table 1. Specifications for Molecular Probes monoclonal antibodies specific for components of the oxidative phosphorylation system.
Catalog
Number of
Antibody
Oxidative Complex
A21344
A21343
A31857
A21359
I (NADH-ubiquinol
oxidoreductase)
A31856
A11142
A21345
II (succinateubiquinol
oxidoreductase)
A21362
A11143
A21346
III (ubiquinol–
cytochrome c
oxidoreductase)
A21361
Species Specificity *
Suggested Conc.
Suggested Conc.
Isotype
for Westerns
for ICC † (μg/ml)
Hum
Bov
Rat
Mou
(μg/ml)
ICC Fixation
Fixation
Method ‡
Antigen
Retrieval
Method §
NA
NA
NR
NA
NA
5
F, (M)
NA
+
5–10
M, (F)
U
–
1–10
F
NA
+
+
0.1–0.5
M
NA
+
+
+
5 –10
M
NA
+
+
ND
+
1– 5
M
T
0.4
+
+
+
+
NR
NA
NA
0.5
+
+
ND
+
NR
NA
NA
0.2
–
+
ND
+
NR
NA
NA
Subunit
Clone
39 kDa
20C11
IgG1,k
0.5
+
+
+
+
NR
30 kDa
3F9
IgG1,k
0.5
+
+
ND
+
20 kDa
20E9
IgG1,k
0.5–1
+
+
ND
+
17 kDa
21C11
IgG2b,k
0.5
+
+
ND
15 kDa
17G3
IgG1,k
0.1
–
+
±
70 kDa
2E3
IgG1,k
0.1
+
+
30 kDa
21A11
IgG2a,k
5
+
Core I
16D10
IgG1,k
0.1
Core II
13G12
IgG1,k
FeS
5A5
IgG2b,k
10 kDa
1H9
IgG2a,k
A6403
I
1D6
IgG2a,k
2
+
+
+
+
2
(M), (F)
NA
A6404
II
12C4
IgG2a,k
2
+
–
±
±
5
(M), (F)
NA
A21347
IV
10G8
IgG2a,k
0.5
+
+
–
–
5 –10
M
NA
A21348
IV
20E8
IgG2a,k
0.5
+
+
ND
ND
NR
NA
NA
A21363
Va
6E9
IgG2a,k
2
+
+
±
+
5
F
T or U
A21349
IV (cytochrome c
oxidase; COX)
Vb
16H12
IgG2b,k
2
+
+
+
+
NR
NA
NA
VIa-L **
14A3
IgG1,k
5
+
–
±
+
NR
NA
NA
VIb
3F9
IgG1,k
1
+
+
+
+
NR
NA
NA
A6401
VIc
3G5
IgG2b,k
2
+
+
+
–
NR
NA
NA
A21367
VIIa-H/L ††
6D7
IgG2a
10
–
+
+
+
NR
NA
NA
A21368
VIIb
2G7
IgG1,k
25
+
+
–
–
NR
NA
NA
A21350
α
7H10
IgG2b,k
0.5
+
+
+
+
1
M
NA
A21351
β
3D5
IgG1,k
0.2
+
+
ND
+
1
F, (M)
NA
A21365
A21366
A21353
A21354
A21355
V (ATP synthase,
F1F0)
d
7F9
IgG2b,k
5
+
+
ND
±
5
F
T or U
OSCP ‡‡
4C11
IgG1,k
0.1
+
+
ND
–
5 –10
F, (M)
NA
Inhibitor
protein ***
5E2
IgG1,k
0.5 †††
+
+
+
+
5 –10 †††
F
NA
* Species specificity: Hum, human; Bov, bovine; Mou, mouse; (+), strong reactivity; (±), weak reactivity; (–), no reactivity. † ICC, immunocytochemistry. ‡ Fixation method:
M, methanol; F, formaldehyde; ( ) = secondary fixation method. § Antigen retrieval method: T, Tris buffer without urea; U, buffer with urea. ** Antibody recognizes subunit
from bovine liver. †† Antibody recognizes subunit from both heart and liver. ‡‡ Oligomycin sensitivity–conferring protein. NR = not recommended, ND = not determined,
NA = not applicable. *** Although not considered a member of Complex V, the ATPase inhibitor protein is associated with this complex. ††† A second protein band (~18 kDa)
has been observed with this antibody in Western blots of Complex V preparations from human heart tissue. It has been suggested by the supplier that the identity of this
protein is Complex IV, subunit IV, and that the reactivity could be tissue specific. We recommend caution when using the antibody for ICC and IHC applications since the
antibody could interact with this protein.
Monoclonal Antibodies Specific for OxPhos Complex IV (COX)
COX catalyzes the transfer of electrons from reduced cytochrome c to molecular oxygen, with a concomitant translocation
of protons across the mitochondrial inner membrane.12,13 This
inner mitochondrial membrane–bound enzyme is composed of
subunits that are encoded in both the mitochondria (subunits I,
II, and III) and the nucleus (ten others). To facilitate the study of
COX structure and mitochondrial biogenesis, Molecular Probes
offers subunit-specific mouse anti–OxPhos Complex IV monoclonal antibodies (Tables 1 and 2). The binding specificity of
our anti–OxPhos Complex IV monoclonal antibody preparations
allows researchers to investigate the regulation, assembly, and
orientation of COX subunits in a variety of organisms.14–18
The antibodies have also proven valuable for analyzing human
mitochondrial myopathies and related disorders as well as
mitochondrial DNA depletion due to drug toxicity.7,10,19,20,21
Alexa Fluor ® 488 and Alexa Fluor ® 594 conjugates of anti–
OxPhos Complex IV subunit I are also available for direct
staining of these COX proteins (Table 3).
Monoclonal Antibodies Specific for Oxidative Phosphorylation
Table 2. Molecular characteristics of components of the oxidative phosphorylation system for which Molecular Probes offers antibodies.
Catalog Number
Complex
Subunit
A21344
A21343
A31857
I
Molecular Weight *
Alternative Name
Protein Number †
Gene Number ‡
39 kDa
42.5
α subcomplex, 9
Q16795
NDUFA9
30 kDa
30.2
Iron-sulfur protein, 3
O75489
NDUFS3
20.0
ND6 protein
P03923
MTND6
20 kDa
A21359
17 kDa
15.5
β subcomplex, 6
O95139
NDUFB6
A31856
15 kDa
15.1
α subcomplex, 6
P56556
NDUFA6
70 kDa
72.7
Flavoprotein
P31040
SDHA
30 kDa
31.6
Iron-sulfur protein
P21912
SDHB
Core I
51.6
None
P31930
UQCRC1
Core II
48.5
None
P22695
UQCRC2
FeS
29.6
Rieske iron-sulfur protein
P47985
UQCRFS1
10 kDa
P13271 §
UCRQ
A6403
I
57
COX I
P00395
MTCO1
A6404
II
25.6
COX II
P00403
MTC02
A21347
IV
19.6
COX IV
P13073
COX4
A21348
IV
19.6
COX IV
P13073
COX4
A21363
Va
16.8
COX Va
P20674
COX5A
13.7
A11142
A21345
II
A21362
A11143
A21346
III
A21361
A21349
IV
9.6 §
Subunit VII
Vb
COX Vb
P10606
COX5B
A21365
VIa-L **
9.5 §
COX VIa
P13182 §
COX6A1
A21366
VIb
10.0 §
COX VIb
P00429 §
COX6B
A6401
VIc
8.8
COX VIc
P09669
COX6C
A21367
VIIa-H/L ††
9.4
COX VIIa
P14406
COX7A2
A21368
VIIb
9.2
COX VIIb
P24311
COX7B
A21350
α
59.8
F1 complex, α subunit
P25705
ATP5A1
β
56.6
F1 complex, β subunit
P06576
ATP5B
d
18.4
None
O75947
ATP5H
A21354
OSCP ‡‡
23.2
F1 complex, O subunit
P48047
ATP5O
A21355
Inhibitor protein (IP)
P01096 §
ATPI
A21351
A21353
V
12.3 §
IF1
* Molecular weight, in kilodaltons, based upon the human protein sequence unless otherwise noted; many of these proteins show aberrant molecular weights when analyzed
by SDS gel electrophoresis. † Protein reference numbers are from SWISS-PROT, an annotated protein sequence database, and refer to the human protein sequence, unless
otherwise noted. ‡ Gene reference numbers are from GenBank. § Protein molecular weight and protein number are for the bovine sequence. ** Antibody recognizes subunit
from bovine liver. †† Antibody recognizes subunit from both heart and liver. ‡‡ Oligomycin sensitivity–conferring protein.
Monoclonal Antibodies Specific for Complexes I, II, III, and V
Molecular Probes offers monoclonal antibodies specific for
individual subunits of Complexes I, II, III, and V (Tables 1 and 2).
When these monoclonal antibodies are used in combination with
the antibodies to Complex IV, the relative levels of all OxPhos
complexes in normal and diseased tissues can be evaluated. The
Table 3. Alexa Fluor® dye conjugates of anti-OxPhos Complex IV, subunit I antibody. *
Catalog Number
Fluorophore
Abs †
Em †
A21296
Alexa Fluor 488
495
519
A21297
Alexa Fluor 594
590
617
®
®
* For best results in immunocytochemistry, an antigen retrieval procedure is
required. † Approximate absorption (Abs) and fluorescence emission (Em)
maxima, in nm, for conjugates.
anti–OxPhos Complex V subunit α (A21350) and a polyclonal
antibody comparable to anti–OxPhos Complex V subunit β
(A21351) have also been shown to mimic angiostatin, a potent
inhibitor of angiogenesis.22
Related Products: Mitochondrial Porin and Mitochondrial
Protein Extracts
Mitochondrial porin is an abundant transmembrane protein
that forms regulated channels (referred to as voltage-dependent
anionic channels, or VDACs) in the outer mitochondrial membrane.23 It forms a small pore (~3 nm diameter) in the membrane, allowing molecules smaller than ~10 kDa to pass.24 Due
to its abundance, porin is often used as a reference standard in
Western blots when assaying for other mitochondrial proteins 9,25
and is an effective marker of mitochondrial mass for immunocytochemistry.21,26 A monoclonal antibody against human porin is
available (A31855).
Monoclonal Antibodies Specific for Oxidative Phosphorylation
For researchers seeking a source of mitochondrial protein
standards, Molecular Probes offers human heart mitochondrial
proteins for SDS-polyacrylamide gel electrophoresis (M22430),
a complete mitochondrial lysate that has tested negative for
hepatitis B and C as well as HIV-1 and -2 in serology tests. This
mitochondrial protein extract is useful for evaluating new mitochondrial protein preparations in 1-D or 2-D gels and for testing
mitochondrial antibodies.
Materials
Contents
Molecular Probes’ unlabeled monoclonal antibodies specific for
subunits of human OxPhos complexes are supplied in unit sizes
of 50 μg and 100 μg. To prepare stock solutions of the lyophilized
antibodies, reconstitute them in 100–250 μL of 1X phosphatebuffered saline (PBS), pH 7.4, containing 1% bovine serum
albumin (BSA).
The Alexa Fluor ® dye–labeled anti–OxPhos IV subunit I
antibodies are supplied as 1 mg/mL solutions in PBS, 0.1%
BSA, 5 mM sodium azide, in unit sizes of 100 μL. The degree
of labeling for each conjugate is typically 2–8 molecules per
IgG molecule. At the time of preparation, the products are certified to be free of unconjugated dyes.
Storage
Upon receipt, store the lyophilized antibodies desiccated at
≤–20°C. When properly stored, these products are stable for at
least one year. Store antibody solutions for up to six months at
2–6°C with the addition of 2 mM sodium azide. For longer
storage, divide the solutions into single-use aliquots and freeze
them at ≤–20°C. AVOID REPEATED FREEZING AND
THAWING.
Upon receipt, store the Alexa Fluor ® dye–labeled anti–OxPhos
Complex IV subunit I antibodies at 2–6°C. When properly
stored, these products are stable for at least six months. For
longer storage, divide the solutions into single-use aliquots and
freeze them at ≤–20°C. AVOID REPEATED FREEZING AND
THAWING, AND PROTECT FROM LIGHT.
Applications
The OxPhos antibodies bind monospecifically to the corresponding proteins of the human oxidative phosphorylation system (Table 1 and Figure 1). These antibodies are versatile and
can be used in a variety of assay formats including solid-phase
binding of native proteins, Western blots of denatured proteins,
and immunocytochemistry of fixed cells.
Because staining protocols vary with application, the appropriate dilution of an antibody should be determined empirically
(see Table 1). For the fluorophore-labeled antibodies, a final
concentration of 1–10 μg/mL should be satisfactory for most
immunocytochemical applications.27 It is a good practice to
centrifuge antibodies briefly in a microcentrifuge to eliminate
any protein aggregates that may have formed during storage and
thus reduce nonspecific staining.
Specifications
The OxPhos monoclonal antibodies were prepared against
the appropriate OxPhos complexes isolated from bovine heart,
bovine liver, and human heart. The following stringent selection criteria were applied during the development of these
antibodies: 1) ability of the antibody to detect native protein by
particle-concentration fluorescence immunoassay (PCFIA) and
enzyme-linked immunosorbent assay (ELISA), 2) monospecificity
of the antibody for denatured protein in Western blots of wholecell extracts and isolated mitochondria, and 3) specific localization of the antibody to mitochondria in fixed cultured human
cells. The purity and yield of each preparation was assessed
with SDS-polyacrylamide gel electrophoresis and quantitative
immunoassay specific for mouse IgG of the appropriate isotype.
The subunit specificity and immunoglobulin isotype are shown
in Table 1. Note: Most of the proteins involved in the oxidative
phosphorylation system are integral membrane proteins, which
contain a large number of hydrophobic amino acids that are
likely to cause the proteins to run aberrantly with respect to
molecular weight in SDS-polyacrylamide gel electrophoresis.
Antigen Retrieval
In immunocytochemistry applications, methanol-fixed or
formaldehyde-fixed and permeabilized target cells may require
antigen retrieval methods before the target cells are blocked and
labeled with antibodies (see Table 1).
1. Perform antigen retrieval by submersing the slide in preheated
(95°C) buffer (10 mM Tris, pH 9.5, 5% urea, or 50 mM Tris,
pH 7.5, without urea) (see Table 1), in a preheated staining jar
and then placing this jar in a boiling-water bath for 20 minutes.
Add boiling chips to the boiling-water bath to prevent spattering.
2. Cool the slide to room temperature, then wash the specimen
three times for 5 minutes each in PBS.
3. Block the specimen in a blocking solution such as BlockAid™
blocking solution (B10710) and/or Image-iT™ FX signal
enhancer (I36933) before proceeding with antibody labeling.
For fluorescence imaging we also recommend ProLong ® Gold
antifade reagent (P36930, P36934) or ProLong® Gold antifade
reagent with DAPI (P36931, P36935).
Monoclonal Antibodies Specific for Oxidative Phosphorylation
References
1. Biochim Biophys Acta 1366, 199 (1998); 2. Biochim Biophys Acta 1366, 211 (1998); 3. Curr Opin Cardiol 13, 190 (1998); 4. Ann Neurol 44, S99
(1998); 5. J Neural Transm 105, 855 (1998); 6. Seminars in Liver Disease 18, 237 (1998); 7. Biochim Biophys Acta 1362, 145 (1997); 8. J Biol Chem
276, 8892 (2001); 9. J Biol Chem 276, 16296 (2001); 10. Biochim Biophys Acta 1315, 199 (1996); 11. Brain 123, 591 (2000); 12. Science 283, 1488
(1999); 13. Annu Rev Biochem 59, 569 (1990); 14. Biochemistry 30, 3674 (1991); 15. Biochim Biophys Acta 1225, 95 (1993); 16. Methods Enzymol
260, 117 (1995); 17. J Biol Chem 268, 18754 (1993); 18. J Biol Chem 266, 7688 (1991); 19. Pediatr Res 28, 529 (1990); 20. Hum Mol Genet 6, 935
(1997); 21. J Hist Cyto 52 in press (2004); 22. Proc Natl Acad Sci USA 98, 6656 (2001); 23. Biochim Biophys Acta 894, 109 (1987); 24. J Biol Chem
273, 24406 (1998); 25. Biochim Biophys Acta 1455, 35 (1999); 26. Mol Biol Cell 9, 917 (1998); 27. Short Protocols in Molecular Biology, 2nd Edition,
F.M. Ausubel et al., Eds., John Wiley and Sons (1992) pp. 14-24–14-30.
Product List
Cat #
A31856
A21359
A31857
A21343
A21344
A21345
A11142
A21361
A21362
A11143
A21346
A6403
A21296
A21297
A6404
A21347
A21348
A21363
A21349
A21365
A21366
A6401
A21367
A21368
A21355
A21351
A21350
A21353
A21354
A31855
B10710
C24430
I36933
P36930
P36931
P36935
P36934
Current prices may be obtained from our Web site or from our Customer Service Department.
Product Name
Unit Size
anti-OxPhos Complex I 15 kDa subunit, mouse IgG1, monoclonal 17G3.........................................................................................................
100 µg
anti-OxPhos Complex I 17 kDa subunit, mouse IgG2b, monoclonal 21C11 *human mitochondrial reactivity*...............................................
100 µg
anti-OxPhos Complex I 20 kDa subunit, mouse IgG1, monoclonal 20E9 *human mitochondrial reactivity*...................................................
100 µg
anti-OxPhos Complex I 30 kDa subunit, mouse IgG1, monoclonal 3F9 *human mitochondrial reactivity*.....................................................
100 µg
anti-OxPhos Complex I 39 kDa subunit, mouse IgG1, monoclonal 20C11 *human mitochondrial reactivity*.................................................
100 µg
anti-OxPhos Complex II 30 kDa subunit, mouse IgG2a, monoclonal 21A11 *human mitochondrial reactivity*..............................................
100 µg
anti-OxPhos Complex II 70 kDa subunit, mouse IgG1, monoclonal 2E3 *human mitochondrial reactivity*....................................................
50 µg
anti-OxPhos Complex III 10 kDa subunit, mouse IgG2a, monoclonal 1H9 ......................................................................................................
100 µg
anti-OxPhos Complex III core 1 subunit, mouse IgG1, monoclonal 16D10 *human mitochondrial reactivity*...............................................
100 µg
anti-OxPhos Complex III core 2 subunit, mouse IgG1, monoclonal 13G12 *human mitochondrial reactivity*...............................................
100 µg
anti-OxPhos Complex III subunit FeS, mouse IgG2b, monoclonal 5A5 *human mitochondrial reactivity*......................................................
100 µg
anti-OxPhos Complex IV subunit I, mouse IgG2a, monoclonal 1D6 (anti-cytochrome oxidase subunit I)
*human mitochondrial reactivity*....................................................................................................................................................................
100 µg
anti-OxPhos Complex IV subunit I, mouse IgG2a, monoclonal 1D6, Alexa Fluor® 488 conjugate
(anti-cytochrome oxidase subunit I, Alexa Fluor® 488 conjugate) *1 mg/mL* *human mitochondrial reactivity*........................................
100 µL
anti-OxPhos Complex IV subunit I, mouse IgG2a, monoclonal 1D6, Alexa Fluor® 594 conjugate
(anti-cytochrome oxidase subunit I, Alexa Fluor® 594 conjugate) *1 mg/mL* *human mitochondrial reactivity*........................................
100 µL
anti-OxPhos Complex IV subunit II, mouse IgG2a, monoclonal 12C4 (anti-cytochrome oxidase subunit II)
*human mitochondrial reactivity*....................................................................................................................................................................
100 µg
anti-OxPhos Complex IV subunit IV, mouse IgG2a, monoclonal 10G8 (anti-cytochrome oxidase subunit IV)
*human mitochondrial reactivity*....................................................................................................................................................................
100 µg
anti-OxPhos Complex IV subunit IV, mouse IgG2a, monoclonal 20E8 (anti-cytochrome oxidase subunit IV)
*human mitochondrial reactivity*....................................................................................................................................................................
100 µg
anti-OxPhos Complex IV subunit Va, mouse IgG2a, monoclonal 6E9 (anti-cytochrome oxidase subunit Va)
*human mitochondrial reactivity*....................................................................................................................................................................
100 µg
anti-OxPhos Complex IV subunit Vb, mouse IgG2b, monoclonal 16H12 (anti-cytochrome oxidase subunit Vb)
*human mitochondrial reactivity*....................................................................................................................................................................
100 µg
anti-OxPhos Complex IV subunit VIa-L, mouse IgG1, monoclonal 14A3 (anti-cytochrome oxidase subunit VIa-L)
*human mitochondrial reactivity*....................................................................................................................................................................
100 µg
anti-OxPhos Complex IV subunit VIb, mouse IgG1, monoclonal 3F9 (anti-cytochrome oxidase subunit VIb)
*human mitochondrial reactivity*....................................................................................................................................................................
100 µg
anti-OxPhos Complex IV subunit VIc, mouse IgG2b, monoclonal 3G5 (anti-cytochrome oxidase subunit VIc)
*human mitochondrial reactivity*....................................................................................................................................................................
100 µg
anti-OxPhos Complex IV subunit VIIa-H/L, mouse IgG2a, monoclonal 6D7 (anti-cytochrome oxidase subunit VIIa-H/L)................................
100 µg
anti-OxPhos Complex IV subunit VIIb, mouse IgG1, monoclonal 2G7 (anti-cytochrome oxidase subunit VIIb)
*human mitochondrial reactivity*....................................................................................................................................................................
100 µg
anti-OxPhos Complex V inhibitor protein, mouse IgG1, monoclonal 5E2 (anti-ATP synthase IP; anti-F1F0-ATPase IP)
*human mitochondrial reactivity*....................................................................................................................................................................
100 µg
anti-OxPhos Complex V subunit , mouse IgG1, monoclonal 3D5 (anti-ATP synthase subunit ; anti-F1F0-ATPase subunit )
*human mitochondrial reactivity*....................................................................................................................................................................
100 µg
anti-OxPhos Complex V subunit , mouse IgG2b, monoclonal 7H10 (anti-ATP synthase subunit ; anti-F1F0-ATPase subunit )
*human mitochondrial reactivity*....................................................................................................................................................................
100 µg
anti-OxPhos Complex V subunit d, mouse IgG2b, monoclonal 7F9 (anti-ATP synthase subunit d; anti-F1F0-ATPase subunit d)
*human mitochondrial reactivity*....................................................................................................................................................................
100 µg
anti-OxPhos Complex V subunit OSCP, mouse IgG1, monoclonal 4C11
(anti-ATP synthase subunit OSCP; anti-F1F0-ATPase subunit OSCP) *human mitochondrial reactivity*.........................................................
100 µg
anti-porin (human mitochondrial), mouse IgG2b, monoclonal 31HL *1 mg/mL*............................................................................................
100 µL
BlockAid™ blocking solution *for use with microspheres*.............................................................................................................................
50 mL
CoroNa™ Red chloride.....................................................................................................................................................................................
1 mg
Image-iT™ FX signal enhancer.........................................................................................................................................................................
10 mL
ProLong® Gold antifade reagent.......................................................................................................................................................................
10 mL
ProLong® Gold antifade reagent with DAPI......................................................................................................................................................
10 mL
ProLong® Gold antifade reagent with DAPI *special packaging*.................................................................................................................... 5 x 2 mL
ProLong® Gold antifade reagent *special packaging*..................................................................................................................................... 5 x 2 mL
Monoclonal Antibodies Specific for Oxidative Phosphorylation
Contact Information
Further information on Molecular Probes products, including product bibliographies, is available from your local distributor or directly from Molecular Probes. Customers in
Europe, Africa and the Middle East should contact our office in Paisley, United Kingdom. All others should contact our Technical Service Department in Eugene, Oregon.
Please visit our website — probes.invitrogen.com — for the most up-to-date information.
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Phone: (541) 465-8300 • Fax: (541) 335-0504
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Phone: (541) 335-0353 • Toll-Free (800) 438-2209
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super­vision of, a tech­nically qualified individual experienced in handling potentially hazardous chemicals. Please read the Material Safety Data Sheet provided for
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For research use only. Not intended for any animal or human therapeutic or diagnostic use. The purchase of this product conveys to the buyer the non-transferable
right to use the purchased amount of the product and components of the product in research conducted by the buyer (whether the buyer is an academic or for-profit
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otherwise use this product or its components or materials made using this product or its components for Commercial Purposes. The buyer may transfer information
or materials made through the use of this product to a scientific collaborator, provided that such transfer is not for any Commercial Purpose, and that such collaborator
agrees in writing (a) to not transfer such materials to any third party, and (b) to use such transferred materials and/or information solely for research and not for Commercial Purposes. Commercial Purposes means any activity by a party for consideration and may include, but is not limited to: (1) use of the product or its components
in manufacturing; (2) use of the product or its components to provide a service, information, or data; (3) use of the product or its components for therapeutic, diagnostic or prophylactic purposes; or (4) resale of the product or its components, whether or not such product or its components are resold for use in research. Invitrogen
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willing to accept return of the product with a full refund. For information on purchasing a license to this product for purposes other than research, contact Molecular
Probes, Inc., Business Development, 29851 Willow Creek Road, Eugene, OR 97402. Tel: (541) 465-8300. Fax: (541) 335-0504.
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Copyright 2005, Molecular Probes, Inc. All rights reserved. This information is subject to change without notice.
Monoclonal Antibodies Specific for Oxidative Phosphorylation