Product Data
Product Data
For Research Use Only. Not For Use In Diagnostic Procedures
For Research Use Only. Not For Use In Diagnostic Procedures
Etoposide
Etoposide
Catalog #: 4886-400-01
Size: 400 μl
Catalog #: 4886-400-01
Size: 400 μl
Description: Etoposide complexes with topoisomerase II and DNA to enhance doublestrand and single-strand cleavage of DNA and reversibly inhibit religation. Etoposide also
blocks the cell cycle in S-phase and G2-phase; induces apoptosis in normal and tumor cell
lines; inhibits synthesis of the oncoprotein Mdm2 and induces apoptosis in tumor lines that
overexpress Mdm2.
Description: Etoposide complexes with topoisomerase II and DNA to enhance doublestrand and single-strand cleavage of DNA and reversibly inhibit religation. Etoposide also
blocks the cell cycle in S-phase and G2-phase; induces apoptosis in normal and tumor cell
lines; inhibits synthesis of the oncoprotein Mdm2 and induces apoptosis in tumor lines that
overexpress Mdm2.
Formula weight: 588.56
Formula weight: 588.56
Physical State: Etoposide is provided in DMSO. The final reagent concentration is 25 mM.
Physical State: Etoposide is provided in DMSO. The final reagent concentration is 25 mM.
Storage:
Storage:
Store at 4°C, shielded from light and with desiccant. For extended
storage, freeze in working aliquots in a manual defrost freezer to avoid
freeze-thaws.
Store at 4°C, shielded from light and with desiccant. For extended
storage, freeze in working aliquots in a manual defrost freezer to avoid
freeze-thaws.
Bring to room temperature before use.
Applications:
Bring to room temperature before use.
Induction of apoptosis in vitro. To induce apoptosis, dilute etoposide in
media to a final concentration ranging from 10 μM to 100 μM. The
concentration and length of treatment are cell type dependant and require
optimization. Please refer to MSDS before handling this product.
Applications:
Induction of apoptosis in vitro. To induce apoptosis, dilute etoposide in
media to a final concentration ranging from 10 μM to 100 μM. The
concentration and length of treatment are cell type dependant and require
optimization. Please refer to MSDS before handling this product.
References:
1. Chow, K.C., and Ross, W.E., 1987. Topoisomerase-specific drug sensitivity in relation to
cell cycle progression. Mol. Cell Biol., 7:3119-3123.
2. Fearnhead HO, M. Chwalinski , R.T. Snowden, M.G. Ormerod, GM.Cohen.1994. Dexamethasone and etoposide induce apoptosis in rat thymocytes from different phases of the
cell cycle. Biochem Pharmacol 48:1073-9.
3. Burden, D.A., et al., 1996. Topoisomerase II-etoposide interactions direct the formation
of drug-induced enzyme-DNA cleavage complexes. J. Biol. Chem., 271:29238-29244.
4. Hande, K.R. 1998. Etoposide: four decades of development of a topoisomerase II
inhibitor. Eur.J. Cancer, 34:1514-1521.
5. Kaufmann, S.H., 1998. Cell death induced by topoisomerase-targeted drugs: more
questions than answers. Biochim. Biophys. Acta, 1400:195-211.
References:
1. Chow, K.C., and Ross, W.E., 1987. Topoisomerase-specific drug sensitivity in relation to
cell cycle progression. Mol. Cell Biol., 7:3119-3123.
2. Fearnhead HO, M. Chwalinski , R.T. Snowden, M.G. Ormerod, GM.Cohen.1994. Dexamethasone and etoposide induce apoptosis in rat thymocytes from different phases of the
cell cycle. Biochem Pharmacol 48:1073-9.
3. Burden, D.A., et al., 1996. Topoisomerase II-etoposide interactions direct the formation
of drug-induced enzyme-DNA cleavage complexes. J. Biol. Chem., 271:29238-29244.
4. Hande, K.R. 1998. Etoposide: four decades of development of a topoisomerase II
inhibitor. Eur.J. Cancer, 34:1514-1521.
5. Kaufmann, S.H., 1998. Cell death induced by topoisomerase-targeted drugs: more
questions than answers. Biochim. Biophys. Acta, 1400:195-211.
© 2007 Trevigen, Inc. All Rights Reserved. Trevigen is a registered trademark of Trevigen, Inc.
© 2007 Trevigen, Inc. All Rights Reserved. Trevigen is a registered trademark of Trevigen, Inc.
8405 Helgerman Court, Gaithersburg, MD 20877 USA
Voice: 1-800-TREVIGEN (1-800-873-8443) • 301-216-2800
Fax: 301-560-4973 • e-mail: [email protected] • www.trevigen.com
P7/11/07v1
8405 Helgerman Court, Gaithersburg, MD 20877 USA
Voice: 1-800-TREVIGEN (1-800-873-8443) • 301-216-2800
Fax: 301-560-4973 • e-mail: [email protected] • www.trevigen.com
P7/11/07v1
Etoposide
Etoposide
Catalog #: 4886-400-01
Storage: 4 °C
Catalog #: 4886-400-01
Storage: 4 °C
1-800-873-8443
1-800-873-8443