Name___________________ Date_________ Period_______ Olson PTC-Lab GL:9-11 What Do You Taste? The ability to taste PTC is a genetically heritable trait that follows Mendelian patterns. To understand this lab you will use your knowledge of genes, alleles, genotypes, phenotypes, dominance and punnett squares: probability and ratios. PTC paper is used to detect the tastebuds that taste bitter. These tastebuds are located at the back of the tongue and in the throat. Unlike sweet, sour and salty taste buds, there are many different types of bitter taste buds. Because of this not everyone has all the same bitter tastebuds. Have you ever tasted something like medicine that leaves an after taste? It’s a bitter taste and you only taste it in the back of the tongue. The same can be done for sour also. You can taste it on the back and sides of your tongue. There is only one type of sour taste bud (detects H+), only one type of salt taste bud (detects Na+), and only one type of sweet taste bud (detects sugar), but there are many types of bitter taste buds. Do you think the ability to taste is likely to differ among people? Yes or No Why do you think different people will be able to taste different bitter tastes? _______________________________ ____________________________________________________________________________________________ Write a hypothesis about the difference in taste ability. Remember to use “If Then, Because” format. ___________________________________________________________________________________________ ___________________________________________________________________________________________ Materials PTC taste strips Control taste strips Mini Zip-lock bags Procedure You will follow these steps to find out which compounds you taste. 1. Take one control taste strip from the bag marked “control”. 2. Place the taste strip on your tongue. Be sure to test all the regions of your tongue. “You should not taste anything.” It should taste like paper. “Remember what it tastes like!” 3. Remove the taste strips from your mouth immediately throw it in the trash can. Do not place it on your lab table, stool or desk! Idea came from Spice by Deena Westbrook & Marbie Caruso. Annotations by: Charlene Olson 6/2010 1 4. Take one PTC taste test strip from the marked bag “PTC”. 5. Place the taste strip on your tongue. Be sure to test all the regions of your tongue. 6. Remove the test strip from your mouth, and immediately throw it in the trash can. Do not place it on your lab table, stool or desk! 7. Record whether or not you taste PTC in your chart. If you tasted nothing, or if it tasted the same as the control test, you are a non-taster. Otherwise, you are a taster. 8. Now you will be given 4 test strips to take home and ask that your parents take the test also. With the two left you may test either 2 family members or your grandparents. Record your finding on the chart and bring the lab handout back to school. Lab Group Members PTC Taster PTC Non-taster 9. Was there a lot of variety in your classmates’ ability to taste? Is this evidence for against your hypothesis? ____________________________________________________________________________________ ____________________________________________________________________________________ ____________________________________________________________________________________ 10. Do you think your family members could taste the same as you, or do you think their taste abilities will differ from yours? Why?______________________________________________________________________ _____________________________________________________________________________________ _____________________________________________________________________________________ Family Members Tested PTC Taster PTC Non-taster 11. What were your findings when testing family members with the PTC? ______________________________ ______________________________________________________________________________________ 12. Is there a relationship between the ability to taste one bitter substance and the ability to taste another? _________________________________________________________________________________ _________________________________________________________________________________ _________________________________________________________________________________ On the back of this handout you will now complete a punnett square of your families’ abilities to taste and see what the probability and ratios could be for a family member to taste the PTC. Idea came from Spice by Deena Westbrook & Marbie Caruso. Annotations by: Charlene Olson 6/2010 2
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