Davidson et al., Supplemental Figure 1
A
B
SKN1 domain
1
121
150
181
Transmembrane helix (150-172)
β-glucan synthesis associated
SKN1 domain (121-179)
C
Sp
Bm
Ca
Cg
An
Af
Cn
Sbg1p
XP_007685256
XP_446183
KGU30817
CBF69971
EDP54700
XP_012049585
0.16
0.063
0.111
0.27
0.149
0.144
0.1485
121
159
160
180
165
163
76
KEVDDILHNEDFYDGRD---------LNIFTLRGFVNILTLILLSCGLLMLFIGYPILSAVEVEKQRKKN
VEKDDYLHNPDPNDKDK-------RDCDVFTRRGIVNVGGLAILALGILMLFVGYPILTFVEKATAPERT
KEDDDYLHNPDPEEEARLDRKRFSNDFKYMDKRSAGGLAGVLFLFIGLGCLFILLPALTY-TGVTEHAKP
KEPDDYLHNPDPVQDAEYEKNRFMHDLKNMDKRSLGGLIGFIILFIAALAVFIILPALTY-SGATNPYHP
VEKDDYLHNPDPSDREG-------E-CDVWNKRGMINVGGLVLLTIGFLVLFIGYPVVTAMKGFEKTQSG
EEKDDYLHNPDPNDKEG-------Q-CDLWSRRGIMNVTGLVLLTLGFLALFIGYPVITAVKGM-RDKSG
PEEDDWMHNPDPIKNHNYDRGTI------FTPRGLINVGCLVMLILCLVTLFAGYPIITHFTDTQIKTNG
181
247
280
332
226
250
162
gi|191123292 S. pombe Sbg1 β-glucan synthesis associated protein
gi|891556976 S. octosporus β-glucan synthesis associated protein
gi|891602747 S. cryophilus β-glucan synthesis associated protein
gi|213410010 S. japonicus β-glucan synthesis associated protein
gi|961817042 Candida galbrata β-glucan synthesis associated protein KRE6
gi|768881745 S. cerevisiae KRE6p
gi|255726114 Candida tropicalis β-glucan synthesis associated protein SKN1
0.11 gi|723168469 Candida albicans β-glucan synthesis associated protein KRE6
0.154 gi|723181220 Candida albicans β-glucan synthesis associated protein SKN1
0.22 gi|58266800 Cryptocoocus neoformans β-glucan synthesis associated protein SKN1
0.26
gi|406695001 Trichosporon asahii var. asahii β-glucan synthesis associated protein SKN1
0.28
gi|406695001 Pnuemocystis carinii β-glucan synthesis associated protein SKN1
0.185
gi|627826051 Bipolaris oryzae β-glucan synthesis associated protein SKN1
0.197
gi|528296488 Blumeria graminis β-1,6-glucan synthetase
0.022 gi|952551258 Aspergillus lentulus β-glucan synthesis associated protein KRE6
0.021 gi|159129586 Aspergillus fumigatus β-1,6-glucan synthetase KRE6
0.07 gi|121715730 Aspergillus clavatus β-1,6-glucan synthetase KRE6
0.0007
0.0008
0.119
gi|914761099 Aspergillus flavus β-1,6-glucan synthetase KRE6
gi|169774877 Aspergillus oryzae β-glucan synthesis associated protein SKN1
gi|259479600 Aspergillus nidulans β-1,6-glucan synthetase KRE6
0.132 gi|816348981 Aspergillus rambellii β-1,6-glucan synthetase KRE6
0.114
gi|525581985 Penicilum oxalicum putative β-1,6-glucan active enzyme
0.047
gi|902274613 Penicilum camemberti putative β-glucan synthesis associated protein SKN1
0.05
gi|953383561 Penicilum digitatum putative β-1,6-glucan synthetase KRE6
0.107
gi|875280035 Coccidioides immitis β-glucan synthesis associated protein SKN1
0.073
gi|859411350 Coccidioides immitis β-glucan synthesis associated protein SKN1
0.22
gi|930439956 Phialophora attae β-glucan synthesis associated protein KRE6
S1 Fig. Sbg1 is a conserved protein involved in glucan synthesis. (A) Schematic of domain organization of Sbg1. (B)
Sequence alignment of the SKN1 domain in S. pombe Sbg1 with β-glucan synthesis associated proteins from other fungi. SpS. pombe; Bm-Bipolaris maydis; Ca-Candida albicans; Cg-Candida galbrata; An-Aspergillus nidulans; Af-Aspergillus
fumigatus; and Cn-Cryptococcus neoformans. Identical and similar (D/E, I/L/V, K/R, N/Q, and S/T) residues compared with
Sbg1 are in red and blue, respectively. (C) Cladogram of SKN1 domains from different fungal species. A phylogenetic
cladogram with branch length (indicated by the numbers) showing the relationship of the Sbg1 SKN1 domain with
homologous β-glucan synthesis associated proteins from other fungal species. Fungal pathogens are in bold. NCBI’s BLAST
and EMBL-EBI’s ClustalW2 were used to generate the cladogram.