Anatrace–Microlytic MCSG2 Formulations
Anatrace Products, LLC
Condition #
1
2
Well
A1
A2
Salt
0.2 M Potassium Sodium Tartrate
0.15 M Potassium Bromide
3
4
5
6
7
8
9
A3
A4
A5
A6
A7
A8
A9
10
11
12
13
A10
A11
A12
B1
0.2 M Ammonium Nitrate, pH 6.3
0.2 M Lithium Chloride
14
15
B2
B3
0.2 M Potassium Acetate
0.2 M Ammonium Citrate Dibasic
0.2 M Sodium Chloride
0.2 M Lithium Sulfate
0.2 M Sodium Chloride
0.2 M Potassium Formate, pH 7.3
Buffer
0.1 M Na2HPO4: Citric Acid, pH 4.2
0.1 M Sodium Acetate: Acetic Acid, pH 4.5
0.1 M CAPS: NaOH, pH 10.5
0.1 M Sodium Cacodylate: HCl, pH 6.5
0.1 M Na2HPO4: Citric Acid, pH 4.2
Precipitant
20 % (w/v) PEG 3350
30 % (w/v) PEG 2000 MME
0.1 M Sodium Citrate: HCl, pH 5
20 % (w/v) PEG 8000
0.8 / 1.2 M NaH2PO4/K2HPO4
1.2 / 0.8 M NaH2PO4/K2HPO4
1.26 M Ammonium Sulfate
10 % (w/v) PEG 3000
20 % (w/v) PEG 3350
20 % (w/v) PEG 6000
0.1 M Sodium Citrate: Citric Acid, pH 5.5
0.1 M MES: NaOH, pH 6.5
20 % (w/v) PEG 3350
20 % (w/v) PEG 3350
40 % (v/v) PEG 600
12 % (w/v) PEG 20000
20 % (w/v) PEG 3350
20 % (w/v) PEG 3350
16
B4
17
B5
0.2 M Ammonium Sulfate
18
19
B6
B7
0.2 M Sodium Formate
20
21
B8
B9
1 M Ammonium Sulfate
0.1 M Bis-Tris: HCl, pH 5.5
20 % (w/v) PEG 3350
2.8 M Sodium Acetate, pH 7.0
60 % (v/v) Microlytic Mix(1), pH 7.0
1 % (w/v) PEG 3350
22
23
B10
B11
1.1 M Sodium Malonate, pH 7.0
0.2 M Lithium Sulfate
0.1 M HEPES: NaOH, pH 7
0.1 M Bis-Tris: HCl, pH 6.5
0.5 % (w/v) Jeffamine ED-2001, pH 7.0
25 % (w/v) PEG 3350
24
25
26
B12
C1
C2
3 M Sodium Chloride
0.8 M Succinic Acid, pH 7.0
0.1 M Bis-Tris: HCl, pH 5.5
27
C3
0.2 M Ammonium Acetate
0.1 M HEPES: NaOH, pH 7.5
28
29
C4
C5
0.2 M Ammonium Citrate Tribasic, pH 7.0
0.2 M Sodium Citrate
30
31
32
C6
C7
C8
33
34
C9
C10
35
36
37
C11
C12
D1
38
39
D2
D3
40
41
D4
D5
42
43
44
D6
D7
D8
1.1 M Ammonium Tartrate Dibasic, pH 7.0
0.2 M Potassium Nitrate, pH 6.9
45
46
D9
D10
1 M Lithium Chloride
0.2 M Lithium Citrate Tribasic
47
48
49
50
D11
D12
E1
E2
51
E3
52
53
54
55
56
E4
E5
E6
E7
E8
57
58
59
E9
E10
E11
60
E12
61
62
63
64
F1
F2
F3
F4
65
66
67
68
69
70
F10
71
72
73
74
F11
F12
G1
G2
0.2 M Sodium Tartrate Dibasic
0.2 M Lithium Nitrate
0.2 M Sodium Phosphate Dibasic
75
G3
0.2 M Sodium Chloride
0.1 M Imidazole: HCl, pH 8
0.4 / 1.6 M NaH2PO4/K2HPO4
76
77
78
79
80
G4
G5
G6
G7
G8
0.2 M Calcium Acetate Hydrate
0.2 M Sodium Chloride
0.2 M Sodium Chloride
0.1 M MES: NaOH, pH 6
0.1 M CHES: NaOH, pH 9.5
0.1 M Sodium Citrate: Citric Acid, pH 5.5
0.1 M Tris: HCl, pH 7
0.1 M Tris: HCl, pH 7
10 % (w/v) Isopropanol
1.26 M Ammonium Sulfate
1 M Ammonium Phosphate Dibasic
15 % (w/v) Ethanol
81
G9
0.2 M Sodium Chloride
0.1 M Na2HPO4/KH2PO4, pH 6.2
82
83
G10
G11
0.2 M Sodium Chloride
0.1 M CAPS: NaOH, pH 10.5
0.1 M HEPES: NaOH, pH 7
20 % (w/v) PEG 1000
20 % (w/v) PEG 8000
30 % (v/v) Jeffamine ED-2001, pH 7.0
84
85
G12
H1
0.2 M Calcium Chloride
0.2 M Calcium Chloride
0.1 M Bis-Tris: HCl, pH 5.5
0.1 M Bis-Tris: HCl, pH 6.5
45 % (v/v) MPD
45 % (v/v) MPD
86
87
88
89
90
H2
H3
H4
H5
H6
0.2 M Potassium Chloride
0.2 M Trimethylamine N-Oxide
0.2 M Ammonium Acetate
0.1 M Succinic Acid, pH 7.0
0.05 M HEPES: NaOH, pH 7.5
0.1 M Tris: HCl, pH 8.5
0.1 M Tris: HCl, pH 8.5
35 % (v/v) Pentaerythritol Propoxylate 5/4 PO/OH
20 % (w/v) PEG 2000 MME
25 % (w/v) PEG 3350
15 % (w/v) PEG 3350
0.8 M Ammonium Sulfate
91
92
93
94
H7
H8
H9
H10
95
96
H11
H12
1.8 M NaH2PO4/K2HPO4, pH 8.2
0.1 M Sodium Cacodylate: HCl, pH 6.5
0.1 M Bis-Tris Propane: HCl, pH 7
30 % (w/v) PEG 8000
35 % (v/v) Microlytic Mix(1), pH 7.0
25 % (w/v) PEG 3350
20 % (w/v) PEG 3350
20 % (w/v) PEG 3350
0.1 M Bicine: NaOH, pH 9
0.1 M Tris: HCl, pH 8.5
0.1 M Bis-Tris Propane: HCl, pH 7
0.2 M Sodium Fluoride
0.2 M Sodium Nitrate
20 % (w/v) PEG 6000
1.5 M Ammonium Phosphate Dibasic
1.8 M Magnesium Sulfate
20 % (w/v) PEG 3350
20 % (w/v) PEG 3350
0.1 M Bis-Tris Propane: HCl, pH 7
0.1 M Tris: HCl, pH 8.5
0.1 M Tris: HCl, pH 8.5
1.3 M Ammonium Tartrate Dibasic
1.4 M Ammonium Tartrate Dibasic
1.5 M Ammonium Sulfate
0.2 M Lithium Sulfate
0.2 M Lithium Sulfate
0.1 M Tris: HCl, pH 8.5
0.1 M CAPS: NaOH, pH 10.5
1.26 M Ammonium Sulfate
2 M Ammonium Sulfate
0.2 M Calcium Acetate Hydrate
0.1 M Sodium Acetate: Acetic Acid, pH 4.5
0.1 M Tris: HCl, pH 8.5
30 % (v/v) PEG 400
3 M Sodium Chloride
2.8 M Sodium Acetate: HCl, pH 7
20 % (w/v) PEG 3350
0.1 M Sodium Citrate: HCl, pH 4
20 % (w/v) PEG 6000
20 % (w/v) PEG 3350
0.1 M Tris: HCl, pH 8.5
0.1 M Bis-Tris Propane: HCl, pH 7
0.1 M Bis-Tris Propane: HCl, pH 7
1.5 M Lithium Sulfate
20 % (w/v) PEG 3350
1.8 M Sodium Acetate, pH 7.0
3.2 M Sodium Chloride
0.1 M Bis-Tris Propane: HCl, pH 7
1.5 M Lithium Sulfate
0.1 M MES: NaOH, pH 6.5
0.1 M Sodium Acetate: Acetic Acid, pH 4.5
0.1 M Sodium Acetate: Acetic Acid, pH 4.5
0.1 M Na2HPO4: Citric Acid, pH 4.2
0.1 M CHES: NaOH, pH 9.5
10 % (v/v) Dioxane
30 % (w/v) PEG 8000
1 M Ammonium Phosphate Dibasic
20 % (w/v) PEG 1000
20 % (w/v) PEG 8000
0.2 M Magnesium Chloride
0.1 M CHES: NaOH, pH 9.5
0.1 M Na2HPO4: Citric Acid, pH 4.2
0.1 M Sodium Cacodylate: HCl, pH 6.5
1 M Sodium Citrate
1.6 / 0.4 M Na2HPO4/K2HPO4
10 % (w/v) PEG 3000
0.2 M Lithium Sulfate
0.1 M Sodium Cacodylate: HCl, pH 6.5
30 % (v/v) PEG 400
0.2 M Sodium Chloride
0.1 M Imidazole: HCl, pH 8
0.1 M Bis-Tris: HCl, pH 6.5
0.1 M HEPES: NaOH, pH 7.5
1 M Ammonium Phosphate Dibasic
3 M Sodium Chloride
3 M Sodium Chloride
1 M Na2HPO4/K2HPO4, pH 6.9
F5
0.02 M Magnesium Chloride
0.1 M HEPES: NaOH, pH 7.5
22 % (w/v) Polyacrylic Acid 5100
F6
F7
F8
F9
0.2 M Sodium Malonate, pH 7.0
0.15 M DL-Malic Acid, pH 7.0
0.2 M Ammonium Phosphate Monobasic
0.1 M Tris: HCl, pH 8.5
0.1 M HEPES: NaOH, pH 7
20 % (w/v) PEG 3350
20 % (w/v) PEG 3350
50 % (v/v) MPD
10 % (w/v) PEG 6000
0.2 M Ammonium Sulfate
1.6 M Ammonium Sulfate
0.2 M Lithium Sulfate
0.2 M Lithium Sulfate
24 % (w/v) PEG 1500, 20 % (v/v) Glycerol
0.1 M Tris: HCl, pH 8.5
0.1 M Sodium Citrate: HCl, pH 4
0.2 M Magnesium Chloride
20 % (w/v) PEG 1000
0.1 M Bicine: NaOH, pH 9
0.1 M HEPES: NaOH, pH 7.5
0.1 M Sodium Acetate: HCl, pH 4.6
10 % (w/v) PEG 20000, 2 % (v/v) Dioxane
30 % (v/v) PEG 400
2.5 M Ammonium Sulfate
20 % (w/v) PEG 3350
0.1 M Tris: HCl, pH 8.5
0.1 M Sodium Acetate: Acetic Acid, pH 4.5
2.5 M Ammonium Sulfate
10 % (w/v) PEG 3000
0.2 M Sodium Chloride
0.2 M Zinc Acetate
20 % (w/v) PEG 3350
20 % (w/v) PEG 3350
20 % (w/v) PEG 3350
2.4 M Ammonium Phosphate Dibasic
Microlytic Mix is comprised of 1.8305 M Malonic Acid, 0.25 M Ammonium Citrate Tribasic, 0.12 M Succinic Acid, 0.3 M DL-Malic Acid, 0.4 M Sodium Acetate Trihydrate, 0.5 M Sodium Formate, 0.16 M Ammonium Tartrate Dibasic.
[McPherson, A. and Cudney, B. (2006) J. Struct. Biol. 156(3), 387-406.] Microlytic Mix at pH 7.0 is titrated with HCl. This mix is available from Anatrace, Product Nos. OPTIMIZE-131 and OPTIMIZE-132.
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