High Quality Taq DNA polymerase
MP Biomedicals (formerly Qbiogene) has manufactured thermostable polymerases for 15 years.
This collective expertise ensures:
• Lot-to-lot reproducibility
The strict quality control procedure guarantees the exact enzyme activity for each produced batch. It also
includes control of contaminants like nickases, endo/exonucleases, ribonucleases, and bacterial/plasmid DNA.
• Results reliability with ultra-pure polymerase
Taq DNA polymerase is highly purified in order to obtain the lowest possible contamination from E. coli DNA and
plasmid DNA. Results indicate that MP Biomedicals Taq DNA polymerase has the lowest amount of contaminating DNA.
• Flexibility for each application
The unique 10x reaction buffer has been optimized for maximum stability and efficiency in any PCR reaction. For
particular applications, other buffers systems are available with low detergent contents or ammonium sulphate
formulation, or densifying agent and dye for direct loading.
• High amplification yield
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Amplification of a 500bp fragment from phagique DNA with 0,5 U of each Taq DNA polymerase
lane 1 - 100 pg ; lane 2 - 1 ng ; lane 3 -10 ng ; lane 4 - 100 ng
“My experience shows that caution is required to the degree of Taq Polymerase purity when bacteria
antibiotic-resistance is tested by PCR. (1)
I have adopted MP Biomedicals (formerly Qbiogene) Taq-Core polymerase because its high-purity
ensures more reliable results.”
Rosa Mª del Campo Moreno.
Hospital Ramon y Cajal
MADRID - SPAIN
(1)
Journal of Antimicrobial Chemotherapy 2007 Sep ; 60(3):702-3
www.mpbio.com
MP Biomedicals Europe, Tel: 00800 7777 9999  email: [email protected]
Taq Core Kit
High Quality Taq DNA polymerase is also available as a complete set of reagents.
Taq Core Kit contains all the reagents (presented as separate items) required for PCR:
• High quality recombinant Taq DNA polymerase
• High purity dNTPs Mix (> 99%)
• Optimized buffers with and without MgCl2
• Separate MgCl2 solution
Each component has been tested in PCR quality control procedure to give the assurance of High Quality
products for this specific application.
Are you doing amplification set up at room temperature?
Or using primer set containing mismatched bases?
Or particularly short?
Do you need to increase yields by minimizing amplification of non-specific products?
You need a Hot Start system.
You need a SurePRIME DNA polymerase!
SurePRIME™ DNA polymerase is a highly purified form of recombinant Taq DNA polymerase that
has been chemically modified. In this inactivate state, the polymerase is incapable of extending primer-dimers or
mis-annealed primer-template associations prior the activation step at 95°C.
• Easy-to-use: set up your 96 well plates or mastermixes at room temperature
• Low background: introduce restriction site or mismatched bases primers
• Highly specific DNA amplification: multiplex or short primers application
PCR assays were carried out using a non-optimised primer pair, amplifying
a well-characterised 400 bp fragment on a human ß-globin gene. Primers are
shortened oligos, with additional non-matched bases at the 5’ end, to create a
restriction site at each extremity of the PCR fragment. SurePRIME™ DNA
polymerase (lane 1: 1 U; lane 2 : 2U; lane 3: 5 U) and classical Taq DNA Pol
(lane 4: 1 U; lane 5: 2 U; lane 6: 5 U) were comparatively tested.
Product
Cat #
Pack size
Pack Included
SurePRIME™
Hot-Start System
EPHSP025
250 U
5U/µl
EPHSP525
5x250 U
5U/µl
10x Incubation Buffer without Mg
25 mM MgCl2
www.mpbio.com
MP Biomedicals Europe, Tel: 00800 7777 9999  email: [email protected]