Arthropod Management Tests 2009, Vol. 34 doi: 10.4182/amt.2009.C13 (C13) GRAPE: Vitis spp. EFFICACY OF INSECTICIDES AGAINST GREEN JUNE BEETLE, 2008 Donn T. Johnson 320 AGRI BLDG, Department of Entomology University of Arkansas Fayetteville, AR 72701 Phone: 479-575-2501 Fax: 479-575-2452 E-mail: [email protected] Barbara A. Lewis E-mail: [email protected] Green June beetle (GJB): Cotinis nitida (L.) Green June beetle (GJB) adults cause feeding damage to ripening apples, blackberries, blueberries, grapes and peaches and larvae feed on green manure or compost applied to soil causing dislodging and desiccation of turf grass and field crop roots. Given that only carbaryl is registered against GJB, the objective of this laboratory test was to compare the efficacy of several synthetic insecticides and biopesticides (OMRI approved for organic farming) registered on fruit against GJB. On 24 Jul, GJBs were collected from fruit on apple trees and maintained on ripe fruit in a screened cage. Plastic window screen measuring 12″ × 6″ was folded on itself to form a 6″ × 6″ pocket screen cage, the two sides were stapled closed, then ten GJBs and a slice of ripe apple were placed inside the cage and the top edge stapled closed. Each treatment cage (replicate) with GJBs and an apple slice was dipped in one of the treatment solutions (5 replicates) and hung on a wire inside the insectary. At 24, 48 and 72 h after dipping, records were made of the proportion of GJB adults per screen cage that were live (able to walk), morbid (probing elicits slight leg movement, but no walking) or dead. Data of the proportion of dead GJB adults that feed on treated grapes were transformed by arcsine square root (proportion), analyzed with analysis of variance and mean separation used the Waller-Duncan k-ratio t-test (raw means reported in the Tables below). At 72 h after treatment on 24 Jul, the caged GJBs dipped in treatment solutions of NAI 2302 and Alverde killed > 87 % of the GJB adults which were similar to Ecotrol (71%) but significantly more mortality than by MOI 201, Botanigard, QRD416 or the water check (all < 41%). Efficacy of Alverde was similar to Ecotrol and Aza-Direct (Table 1). The treatments dipped on 25 July with Actara, Admire, Altacor, Battalion, Baythroid, Clutch, Danitol, and Sevin all killed 100% of the GJB adults which were significantly greater than the < 51% killed by Ecotrol, Botanigard, Delegate and the water check (Table 2). The last treatments of Aza-Direct or QRD-416 applied on 30 Jul did not differ from the water check (Table 3). Table 1 Treatment or Formulation Active ingredient(s) NAI 2302 15 EC Alverde Tolfenpyrad metaflumizone + 0.5% MSO v/v 10% rosemary oil + 2% peppermint oil azadirachtin 1:500 dilution extract of Chinese medicinal plants plant oil of Chenopodium ambrosioides water Beauveria bassiana Ecotrol EC Aza-Direct 1.2% MOI 201 QRD-416 25% Check Botanigard ES per 100 gpa % dead after 72 h 27 fl oz 16 fl oz 100.0a 87.3ab 96 fl oz (3 qts) 70.9a-c 32 fl oz (2 pts) 25.6 fl oz 50.8b-d 40.9c-e 96 fl oz (3 qts) 9.1de --64 fl oz (2 qts) 5.0e 0.0e Means not followed by the same letter are significantly different (Waller-Duncan k-ratio t-test, k-ratio = 100). Data transformed by arcsine square root (proportion). 1 Arthropod Management Tests 2009, Vol. 34 doi: 10.4182/amt.2009.C13 Table 2 Treatment or Formulation Active ingredient(s) Actara 25% Admire 2F Altacor 35% ai Battalion 0.2 EC Baythroid 12.7% Clutch 50 WDG Danitol 2.4 EC Sevin 4F Ecotrol EC thiamethoxam imidacloprid chlorantraniliprole deltamethrin cyfluthrin clothianidin fenpropathrin carbaryl 10% rosemary oil + 2% peppermint oil water Beauveria bassiana spinetoram Check Botanigard 22 WP Delegate 25 WG per 100 gpa % dead after 72 h 3.5 oz 32 fl oz 4.5 oz 14.1 fl oz 3.2 fl oz 3 oz 10.677 fl oz 64 fl oz (2 qts) 96 fl oz (3 qts) 100.0a 100.0a 100.0a 100.0a 100.0a 100.0a 100.0a 100.0a 50.0b --32 oz (2 lbs) 5 oz 12.0c 4.0d 2.0d Means not followed by the same letter are significantly different (Waller-Duncan k-ratio t-test, k-ratio = 100). Data transformed by arcsine square root (proportion). Table 3 Treatment or Formulation Active ingredient(s) QRD-416 25% plant oil of Chenopodium ambrosioides azadirachtin water Aza-Direct 1.2% Check per 100 gal sol./A 96 fl oz (3 qts) % dead after 72 hrs 30.0a 32 fl oz (2 pts) --- Means not followed by the same letter are significantly different (Waller-Duncan k-ratio t-test, k-ratio = 100). Data transformed by arcsine square root (proportion). 2 0.00a 0.00a
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