expert reviews
http://www-ermm.cbcu.cam.ac.uk
a
t = 4 weeks
Allogeneic skin graft
CBA-hCD2
(CBA mouse
transgenic for
human CD2)
t=0
Remove
thymus
hCD2
Skin graft survives
indefinitely
(animal is tolerant)
t = 4 weeks
Inject with
anti-CD4 and
anti-CD8 antibodies
(non-depleting)
b
CBA mouse
(naive)
Tolerant
CBA mouse
with long-term
skin graft
'Test tube' CBA mouse
(no T cells of own)
Spleen cells
Spleen cells
[including tolerant
(regulatory) hCD2+
T cells]
Tolerant
hCD2+ T cells
removed at
7 days
Skin graft is rejected
by co-injected naive T cells
(hCD2+ cells are removed by
giving anti-hCD2 antibody)
Tolerant
hCD2+ T cells
removed at
14 days
Tolerant status is
'challenged' with
new allogeneic
skin graft of donor strain
Skin graft is accepted
Co-injected naive T cells
are unable to reject graft
'Infectious' transplantation tolerance: naive T cells become tolerant by
prolonged exposure to tolerant T cells
‘Infectious’ transplantation tolerance: naive T cells become tolerant
by prolonged exposure to tolerant T cells
in molecular medicine
Expert Reviews in Molecular Medicine © 1999 Cambridge University Press
Figure 5. ‘Infectious’ transplantation tolerance: naive T cells become tolerant by prolonged exposure
to tolerant T cells (see next page for legend ) (fig005abo).
Accession information: (99)00122-2a.pdf (short code: fig005abo); 29 October 1999
ISSN 1462-3994 ©1999 Cambridge University Press
1
expert reviews
in molecular medicine
Figure 5. ‘Infectious’ transplantation tolerance: naive T cells become tolerant by prolonged exposure
to tolerant T cells. The same tolerance induction protocol (as in Fig. 4) was applied to transgenic mice that
expressed human CD2 (hCD2) on all of their T cells. (This model allowed the selective removal of the tolerant
T cells at different times after injection of naive recipient-strain cells.) When the tolerant and naive cells were
allowed to co-exist in the recipients for 7 days before removal of the tolerant hCD2+ cells, fresh skin grafts
were rejected. However, when the cells were allowed to co-exist for 14 days, removal of the tolerant T cells did
not lead to rejection. These experiments suggest that the naive T cells become tolerant as a result of prolonged
exposure to tolerant T cells (fig005abo).
Accession information: (99)00122-2a.pdf (short code: fig005abo); 29 October 1999
ISSN 1462-3994 ©1999 Cambridge University Press
‘Infectious’ transplantation tolerance: naive T cells become tolerant
by prolonged exposure to tolerant T cells
http://www-ermm.cbcu.cam.ac.uk
2