ALLCELLS
We Sell Cells
Basophils • B Cells • Dendritic Cells • Eosinophils • Epithelial Cells • Erythroid
Progenitor Cells • Fibroblasts • HUVEC • Keratinocytes • Lymphoid Cells
Macrophages • Marrow Stromal Cells • Monocytes • Mononuclear Cells • Myeloid
Cells • Neutrophils • NK Cells • Plasma Cells • Platelets • Red Blood Cells •
Stem/Progenitor Cells • Diseased Cells • B Cell Lymphoma • Multiple Myeloma
Who is AllCells
AllCells is a global biotechnology company founded in 1998
to provide researchers a reliable supply of primary human
cells to advance important scientific research projects in
cell biology, oncology, virology, hematology, immunology,
infectious disease and stem cell research.
For over ten years AllCells has provided researchers the cell quality, purity and viability they
depend on for important research projects. We use innovative techniques to isolate and select
exact cell types based on research needs.
By partnering with AllCells investigators can dramatically reduce time needed to isolate cells
and provide guaranteed consistency, which improves the efficiency and accuracy of your study.
This not only provides better results, but it makes your work more profitable.
AllCells operates a privately owned, high capacity human cell collection facility managed by
highly trained clinicians to provide customers with a consistent, reliable source of blood and
bone marrow from a large, diverse donor pool. Researchers can review donor samples and
select specific donors based on age, race, treatment history, disease, and phenotypes.
AllCells is fully certified in accordance with local, state and federal requirements. Our products
are obtained from donors participating in an IRB (Institutional Review Board) certified facility.
All collections are performed under approved methods for screening HIV, Hepatitis B and
Hepatitis C before donation to ensure product safety standards.
Recent product additions include cancer cells and molecular products such as comprehensive
RNA (containing the microRNA) and cDNA. We can also provide custom research services to
meet the needs of innovative biotechnology and academic research projects.
It’s just that simple: We Sell Cells.
Normal Peripheral Blood
Mobilized Peripheral Blood Cord Blood
Fetal Liver
Primary Cultured Cells
Highly purified primary cells are cultured in cell-type serum-supplemented media. After
culture, the desired cells are harvested; if necessary, the cells are purified further, using
immunomagnetic MicroBead methods. FACscan analysis is used to verify cultured cell
properties. Cultured cells are available in OptiCell® plate or frozen formats.
Lysates
AllCells provides fresh primary cells that have been lysed and frozen in TRIZOL® at
1-5x106/mL or 50-100x106/5mL concentration ranges. The lysates are mixed thoroughly
and kept at -80°C. Lysate products are ideal for making RNA and cDNA but not for
protein purifications.
AllCells Comprehensive RNA (cRNA) includes a combination of Total RNA(>200nt)
small and micro RNA (19-200nt) from viable human cells. These cells are isolated from
human blood, bone marrow or umbilical cord blood, using immunomagnetic MicroBead
labeling procedures. The purity of the cells is analyzed by FACS assay and is higher
than 90%. AllCells uses an optimized phase extraction method to isolate the freshly
purified cells, which are lysed with TRIZOL® reagent.
AllCells have the ability to collect specific cell types from a variety of tissues:
AllCells provides a wide range of primary hematopoietic cells, which are available
from numerous sources including normal peripheral blood (NPB), G-CSF mobilized
peripheral blood (MPB), bone marrow (BM), cord blood (CB), and fetal liver (FL).
Human skin cells and non-human primate primary cells are available as well.
Comprehensive RNA
Products
Primary Cells
Bone Marrow
Through these tissue types, we can isolate MNC, HLA-A2+ MNC, Platelets, Basophils, Eosinophils, Neutrophils,
Monocytes, B Cells, T Cells, NK Cells, Early Progenitor Cells, Marrow Stromal Cells, and many more.
Positive selection process: cells of interest are magnetically labeled with MicroBeads and isolated as the magnetically
retained cell fraction. This is the most direct and specific way to isolate target cells from heterogeneous cell
suspensions. We guarantee that positively selected cells have a purity of ≥90%.
Negative selection process: also known as untouched cell isolation is the depletion of unwanted cells. Non-target cells
are magnetically labeled and eliminated from the cell mixture by immunomagnetic separation. The non-magnetic,
untouched cell fraction contains the target cells. Untouched cells are less likely to be activated by antibodies. We
guarantee that the cell purity is ≥85% in this case.
We partner with clinics and hospitals around the country to collect diseased cells from patients such as:
Leukemia (ALL, AML, CLL, CML)
Multiple Myeloma
Burkitt’s Lymphoma
Diffuse Large B Cell Lymphoma
Follicular Cell Lymphoma
Mantle Cell Lymphoma
Non-Hodgkin’s Lymphoma
Waldenström’s Macroglobulinemia
Polycythemia Vera
With our diseased cell collection process we can provide you with patient information prior to purchase. Depending on
the stage of the cancer, we can also provide cells in untreated and relapsed/refractory state in Frozen, RNA, or cDNA
formats.
For more information or to order: Phone: 1-888-535-3444 | Fax: 510-450-3903 | E-mail: [email protected]
Every qualified lot of AllCells’ Comprehensive RNA product has high purity and
integrity. Each lot has been validated by the Agilent 2100 Bioanalyzer. In addition, the
Comprehensive RNA product is shown to have intact tRNA, 5s, 5.8s, 18s, and 28s rRNA,
and an extremely minimal presence of genomic DNA.
Total RNA
AllCells human primary cell Total RNA is highly purified from viable human cells. These
cells are isolated from human blood, bone marrow, or umbilical cord blood, using
immunomagnetic MicroBead labeling procedures. The cell purity is analyzed by FACS
assay and is generally higher than 90%.
Freshly purified cells are lysed in RNase-free conditions. Total RNA is isolated with an
optimized RNA purification method. Residual genomic DNA is further removed with
RNase-free Dnase I.
cDNA
Human primary cell cDNA is synthesized from highly purified RNA, which is derived
from high purity, hematopoietic cells. These cells are isolated from human blood,
bone marrow, or umbilical cord blood, using immunomagnetic MicroBead labeling
procedures.
RNA is purified from the cells by using an optimized procedure to ensure that the
cDNA is of highest quality and free of genomic DNA contamination.
Cell
Formats
Delivery
Information
AllCells guarantees the cells are properly collected and shipped
on-time. The cells are shipped in specific shipping medias
formats for prompt delivery. Please allow 1-2 weeks lead time
to process the order before your experiment. With overnight
shipping service, you will get your order on time. Here are the
examples of cell types, fresh and frozen products:
Cell type
Fresh
Frozen/Lysates
Examples
Whole tissue,
Platelets
Transferred to a conical tube
containing DPBS buffer.
Shipped at room temperature
Lysates are packaged in dry ice
at shipment
Bone Marrow
Plasma
Shipped at room temperature
Snap Frozen. Packaged in dry
ice at shipment
NPB-Plasma
Mononuclear
Cells
Transferred to a 50mL conical
tube with shipping media
(1xDPBS + 0.5% BSA + 2mM
EDTA). Packaged with a cold
gel pack.
Frozen MNC are stored in a
solution of 50% IMDM, 40%
FBS, 10% DMSO (0.2µm filtered)
in liquid nitrogen storage until
ready for shipment.
Cord Blood, MNC,
BM-MNC, NPBMNC, MPB-MNC
Purified Cells
Transferred to a small
centrifuge tube containing
shipping media. Packaged
with a cold gel pack.
Frozen cells are stored in a
solution of 50% IMDM, 40%
FBS, 10% DMSO (0.2µm filtered)
in liquid nitrogen storage until
ready for shipment. Packaged in
dry ice at shipment
CD34+ cells,
Basophils, T Cells,
B Cells, NK Cells,
Monocytes
Cultured Cells
Cells are cultured and
transported in an OptiCell
plate that has a two 50cm2
gas permeable, optically clear
growth membranes.
Frozen cells are stored in a
solution of 50% IMDM, 40%
FBS, 10% DMSO (0.2µm filtered)
in liquid nitrogen storage until
ready for shipment. Packaged in
dry ice at shipment
HUVEC, CVEC,
MSC, Fetal Liver
Stromal Cells
RNA
N/A
Stored in a 500µL microtube
with 20 to 100µL RNase water.
Packaged in dry ice at shipment
ABM-CD34+
Comprehensive
RNA
Courier
Packages are
shipped using
FedEx Priority
Overnight service
with a delivery
commitment of
10:30am local
time. Same day
deliveries are
available upon
request.
AllCells Certificate of Analysis
Counts
With every product order AllCells will provide a
detailed Certificate of Analysis that contains:
•
•
•
•
•
•
•
Catalog number
Catalog description
Cell Count
Cell viability
Cell purity
Donor information (as provided)
Viral results (HIV, HBV, HCV)
100
50
Additionally, each purified cell product package
contains a FACScan Dot Plot such as shown to the
right. The figure is a depiction of a FACScan dot plot
for CD34+ Progenitor Cells. The dot plot depicts
a logarithmic measurement of cell surface marker
expression intensity by a fluorescent antibody versus
cell number. The left peak represents the control and
the right peak shows positive specificity.
For more information or to order:
Phone: 1-888-535-3444
Fax: 510-450-3903
E-mail: [email protected]
AllCells, LLC
5858 Horton St, #360
Emeryville, CA 94608
www.allcells.com
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ALLCELLS