Sec.: Name: Experiment: _ Dihybrid Crosses (B. Science 10-11-3a) rurpose: To diagram inheritance in dihybrid ~rosses. ~aterials: brain ~ethods: ~art A: Dihybrid Cross of Horses !. In horses, black coats and trotting gait are lominant while the recessive alleles are white Ind pacing gait. If the male is homozygous for ~oth dominant traits what is the genotype of the hale? What genes are present in the sperms of this hale? 3. If the female is Yecessive for both traits, what genes are present in the eggs? 4. What is the genotype of the F1 generation? 5. What is the phenotype of the F1 generation? 6. Assuming a male and female are produced in the F1, use these as parents to produce the F2 generation. Diagram the possible inheritance of the offspring in the Punnett square in results. Then list the ratio of the phenotypes. 7. Would a dihybrid cross of this type always produce the same phenotype ratio? Explain 8.. Summarize the genotype ratios of the F2 offspring in results. Part B: Dihybrid Crosses of Corn Plants 1. In corn plants, rough seed shape (R) is dominant over smooth seed shape (r) and y~3110w seeds (Y) are dominant over white seeds (y). Determine the probable seeds slnape and color of the F1 generation of offspring whose parents are heterozygous for the traits by completing the Pun nett square in part B of results. 2. After you have completed the Punnett square in part B, fill in the data table in part B. t Besults: Part A: Dihybrid Cross 1. genotype: 2. genes: 3. genes: 4. genotype of F1: 5. phenotype: 6. __ black - trotters black - pacers white - trotters white - pacers 7. __ , why 8. BBTT _ BbTT _ BBTt _ BbTt _ BBtt Bbtt of Horses _ Females_ Genes Male ~ _ bbTT bbTt bbtt Sec.: Part B: Dihybrid P, generation RrYy I Crosses of Corn . _Name: ._ Plants RrYy Female gametes A RY Ry rY ry Ry JAY 2:16 Phenotypl,: Genotypic Ratio Phenotype Ratio smooth. yellow seed 1:1€ Genotype rrYy - ..~~ l:1 RRyy I rrYV RrYV rryy Rryy RRYy RrYy I Conclusions: 1. In a cross of two individuals heterozygous for a single would be obtained? trait, what genotype ratio 2. What phenotype ratio would be obtained? 3. What phenotype ratio is obtained when individuals heterozygous for two traits are crossed? 4. Explain how Mendel came up with the Law of Independent Assortment. 5. Explain if the Law of Independent Assortment would apply if the genes for coat color and gait in horses were carried on the same chromosomes (meaning the genes would be physically connected) 6a. As we study inheritance of traits we must keep in mind that because of the fall, back in the Garden of Eden, we inherit a "sinful nature". Quote the first portion of Rom. 13: 14 to comment on what we should do to not "gratify the desires of the sinful nature". 6b. Quote a portion of Gal. 5: 24 to comment on what Jesus has done to our "sinful nature" when we belong to Christ Jesus. 6c. Quote a portion of 2 Cor. 5: 17 to comment on what happens to our old inherited sinful nature when we are "in" Christ. SELECTED TRAITS IN CATS Trait Dominant Allele Recessive Allele Coat length Tabby stripes Tabby (T) Colorpoint (n) Colclrpoint (markings on nose, ears, paws, and tail) ~how th~ re~ultsof the follow~ngc.rosses using Punnett squares ~nd t~e I ' mformatlOn In .the accoIlJPanymg hgyre. ( u r--e,.. (P"'e-.II~.r +p I f'"\ vluC;I /\-e..eJ' e.. D;, (~11' 1ev'\O+~plG. 41\d' p~eI10T:JP[c ~o.s) lvo:,'VfC/'l.esc.J>r ~ l. Heterozygous 'short-hair X h H peterozygous short-hair +~ 6e.s h H Genotypic ratio: Phenotypic ratio: 2. Heterozygous tabby X stripeless t t t Genotypic ratio: ' Phenotypic ratio: 3. Colorpoint X homozygous normal Genotypic ratio: , Phenotypic ratio: -"-,,.- Name _ -. 4. Homozygous short, homozygous . colorpoint ~... X homozygous long, homozygous normal hN '~" r -,...-, -.. , , -- -Hn Hn Hn --' , -. -"!. hN hNhN Genotypic ratio: Phenotypic ratio: - hn hN ... ' HN Hn ,. --, -.j .' '- 5. Heterozygous short, heterozygous normal X heterozygous short, heterozygous normal .. ' ..-J__ ~, -+. 1 , - Genotypic ratio: Phenotypic ratio: ' 6. Heterozygous tabby, heterozygous tn stripeless colorpoint normal X '-0- II i I I - I ; tn - -j I L1 ,Genotypic ratio: Phenotypic ratio: 7. Long-hair, heterozygous Long-hair, heterozygous ----_.\ normal X normal .. '. hN hN .. Genotypic ratio: Phenotypic ratio:
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