AP.PNP #
AP.PCP #
Journal of
ATP Analogues at a Glance
Cell Science
TEMPO-N6-ATP
Clive R. Bagshaw
AP.PCCl2P
Cy3-N6-ATP
Amino extensions
Base modified
APCP.P
ACP.PP
O Substitution
Biotin-17-ATP #
NH2
N
AT P γF
OH
Phosphate modified
ATPαS
HO
OH
OH
P
O P
O P
O
O
O
N
N
O
CH2
H
N
H
O
Formycin TP
Key
ATPβS
H
H
HO
ATPγS # •
OH
Cy3-EDA-ATP
NPE-ATP #
2-SH-ATP •
2-MeS-ATP #
2-Cl-ATP #
Ring substitutions
Cou-EDA-ATP
DMNPE-ATP #
2-Amino purine TP
Flourescent
Luminescent
NMR probe
EPR probe
Electron microscopy
Non- or slowly hydrolysable
Transition-state analogue
Photoaffinity label *
Photoactivatable
Precurser •
Commercially available #
2-F-ATP
Caged ATP
8-Br-ATP #
Cy5-EDA-ATP
Desyl-ATP #
2′(3′) Ribose modified
8-Azido-ATP *
Alexa532-EDA-ATP
8-APAS-ATP *
amino-ATP •
EDA-ATP •
γAmNS-ATP #
MABA-ATP
FEDA-ATP
γ-AEDANS-ATP
Probes
mant-ATP #
Bz2-ATP *#
Bodipy FL-EDA-ATP #
2′-DNS-ATP
λ-Thio-Bodipy-ATP #
Ring extensions
ant-ATP
ε-ATP •#
Caged FEDA-ATP
ε-aza-ATP
Bodipy TR-EDA-ATP #
ADP.SO4
TNP-ATP #
TEMPO-Bz2-ATP *
ADP.Vi
Pi analogues
REDA-ATP
ADP.BeF3
Tb-chelate-EDA-ATP
ADP.AlF4
DEDA-ATP
Co.ATP
Metal substitutes
Cr.ATP
© Journal of Cell Science 2001 (114, pp. 459-460)
Application
Spectroscopic Probes
Reaction kinetics
Fluorescence microscopy
LRET
NMR
EPR
Electron microscopy
Site labelling
Perturbants
Non-hydrolysable
Slowly hydrolysable
Transition-state analogue
Stereochemistry
Photoactivatable
Caged ATP
Caged fluorophore
Photoaffinity label
Example
mant-ATP
Cy3-EDA-ATP
Tb-chelate-EDA-ATP
ATPγF, 2F-ATP
TEMPO-ATP
2′,3′-TEMPO-ATP
Biotin 17-N6 ATP
AP.PNP
ATPγS
ADP.Vi
ATPαS
NPE-ATP
Caged FEDA-ATP
Bz 2 -ATP
549
564
650
666
218
0.14
0.7
0.2
1