Medium
Item No.
Grade
Size
Agar-A
Agar-A is bacteriological grade agar of highest quality.
After autoclaving, a solution of 1.5% Agar-A is light
amber color completely free of insoluble particulate.
Agar-A solidifies into a haze-free, firm-surface gel.
Color: white or light cream. pH: 6.0-7.5 ( 1.5% of
solution). It is suitable for microbiological and tissue
media.
Agar-B
Agar-B is pharmaceutical grade, Agar-B solidify into a
haze-free, firm-surface gel. Color: white or light cream.
pH: 6.0-8.0 (1.5% solution). It is most useful in
applications where clarity is not strictly required. It is
suitable for microbiological and tissue media.
Biotech
100g
500g
Biotech
100g
500g
G1253
Store 18～25℃
AGAR-C
Agar-C is technical grade, 150 mesh powder. Agar-A
solidifies into a haze-free, firm-surface gel. Color:
white or light cream. pH 6.0-8.0 ( 1.5% solution). It is
suitable for inoculating, streaking or replica plating. It
is most useful in applications where clarity is not
required.
Biotech
100 g
500 g
G243
Store 18～25℃
Agar-D (Purified Agar)
Agar-D is a purified agar, is completely nutrient-free,
most often used in defined media formulations where
traces of nutrients can not be tolerated. Some of labs
use this purified agar as substitute of agarose.
Biotech
100g
500g
Biotech
100g
500g
Biotech
[9000-07-1]
500g
Biotech
100g
500g
Biotech
[9000-71-9]
500g
Biotech
[8002-48-0]
100g
500g
FB0010
Store 18～25℃
FB0012
Store 18～25℃
BB0114
Store 18～25℃
CN1138
Store 18～25℃
CB3060
Store 18～25℃
CN5204
Store 18～25℃
MJ873
Store 18～25℃
Name
Agarose see biochemical section P
Agarose low melting see biochemical section P
Beef Extract
A completely water soluble powder. Total nitrogen
approx: 12.5%
Carrageenan
Blended from various seaweeds to produce flexible
gels.
Casamino Acid
Acid hydrolyzed casein; rich in amino acids except
tryptophane. Excellent as a supplement for rich
media.
Total Nitrogen ≥8.7%. Amino Nitrogen ≥5.3%.
Casein
Gelatin see biochemical section P
Malt Extract
Used for the preparation of media for the detection of
yeasts and molds.
pH(6%, water)@25℃: 5.0-6.4 Total Nitrogen <2.0%
Standard Plate Count <5,000/g Coliform <10/g
Salmonella: None Detected
1
NJ853
Store 2～8℃
G213
Store 18～25℃
G214
Store 18～25℃
NZ-Amine A
Used for the preparation of media for the growth of
lambda phages.
pH: 6.4-7.0 Total Nitrogen >11.0% Standard Plate
Count: 10,000/g
Peptone-A (From Meat)
Raw material species: Bovine/Porcine
Total Nitrogen >10% Amino Nitrogen >2%
pH@25℃: 6.5-7.5 Standard Plate Count <5000
CFU/g
Peptone-B (Soy Protein Enzymatic Hydrolysate)
Total Nitrogen >8% pH@25℃: 6.5-7.5 Color
Biotech
100g
500g
Biotech
100g
500g
Biotech
100g
500g
(%Trans@450nm) >25%
G292
Store 18～25℃
Peptone-C (Peptonized Milk) Microbiological Grade
Total Nitrogen >6% Amino Nitrogen >1.9%
pH@25℃: 6.5-7.5 Color (%Trans@450nm) >60%
Biotech
100g
500g
G241
Store 18～25℃
Peptone-D (Protease peptone) Microbiological Grade
Total Nitrogen >10%
pH@25℃: 6.5-7.5
Biotech
100g
500g
Biotech
100g
500g
Biotech
100g
500g
Biotech
100g
500g
Tryptone Powder (Casein Peptone)
Pancreatic digest of casein. Raw Material Species:
Bovine
Total nitrogen >10.0% Amino Nitrogen >2.0% pH: 6.57.5.
Standard Plate Count <5,000 CFU/g
Yeast Extract
May contain black specks. Cell culture tested.
Biotech
100g
500g
Biotech
[8013-01-2]
500g
S507
Store 18～25℃
Yeast Nitrogen Base, without Amino Acids
Used for yeast classification based on amino acid and
carbohydrate requirements.
Biotech
100g
500g
S505
Store 18～25℃
Yeast Nitrogen Base, without Amino Acids &
Ammonium Sulfate
Used for yeast classification based on carbon and
nitrogen requirements. pH(1:10)@25℃: 4.3-4.7
Biotech
100g
500g
Peptone Agar: Passes USP Peptone Test.
G212
Store 18～25℃
G256
Store 18～25℃
G258
Store 18～25℃
Peptone-E (Gelatin Peptone) Microbiological Grade
Total Nitrogen: >10% pH@25℃: 6.5-7.5 Color
(%Trans@450nm): >58%
Peptone F (Lactalbumin Hydrolysate)
Raw material species: Bovine Total Nitrogen >10%
Amino Nitrogen >4% pH(2% solution @25℃): 6.5-7.5
Standard Plate Count <5,000 CFU/g
Polypeptone
Total Nitrogen: >10%. pH@25℃: 6.5-7.5 Tryptophan:
slightly positive. Color (%Trans@450nm) >13%
TG217
Store 18～25℃
G0961
Store 18～25℃
2
Premixed Medium
A MEDIUM
A Medium Broth is a minimal medial used for cultivation and propagation of Escherichia coli. The addition of
glycerol after autoclaving provides the bacteria with a carbon and energy source upon which the bacteria can
grow. Salts supply nitrogen, phosphorus, and trace metals the bacteria would need during its growth cycle.
Magnesium sulfate supplies the magnesium ions required for enzymatic replication of DNA.
Item No.
Name
Component
Size
DJ1018
A Medium Broth
Formula (per Liter):
Ammonium sulfate: 1.0g
Potassium phosphate, dibasic: 10.5g
Potassium phosphate, monobasic: 4.5g
Citric acid, trisodium, dihydrate: 0.5g
pH(25oC): 8.0+0.5
Additional supplements to be added to broth
after autoclaving: 10ml of 20% glycerol
solution, 1ml of 1M MgSO4
100 g
500 g
H Medium Broth
H Medium Broth is moderately rich, general-purpose media that are used for growing E.coli. It contains a large
quantity of tryptone to supply the growing organisms with the basic nutrients and growth factors needed to
support growth. Sodium chloride is added to the mixtures in various concentrations to help maintain the proper
isotonic enviroment for the organisms during their growth phase.
Item No.
Name
Component
Size
DJ1019
H Medium Broth
Usage: 18.0g/L, pH@25℃: 6.8-7.2
100 g
Contents/L：Tryptone 10.0g，Sodium
Chloride 8.0g.
HYLB Broth
HYLB is a medium for obtaining rapid growth of E coli and high yields of plasmid DNA (generally 3-7 times higher
than regular LB medium).
Item No.
Name
Component
SD7001
HYLB Broth
Usage: 43.0g/L
Size
100 g
Lambda Broth, Biotechnology Grade
Used for propagation of lambda phage. The broth contains a large quantity of tryptone, which will provide the
growing organisms with the basic nutrients, and growth factors needed to support growth. Sodium chloride is
added to the mixture in various amounts to help maintain the proper isotonic environment for the bacteria
during growth phase.
Contents/L：Tryptone 10.0g; Sodium chloride 2.5g. Final pH: 7.0 + 0.2.
Item No.
Name
Component
Size
SD7029
Lambda Broth
Usage: 12.5g/L
100 g
LB Broth
Used for maintenance and propagation of E.coli. Microbiologicaly tested.
Contents/L：tryptone 10.0g; yeast extract 5.0g; NaCl 10.0g.
Item No.
Name
Component
Size
SD7002
(S518)
LB Broth
Usage: 25.0g/L.
250 g
LB Broth Agar
Used for maintenance and propagation of E.coli. Microbiologicaly tested.
Contents/L：tryptone 10.0g; yeast extract 5.0g; NaCl 10.0g, Agar 15.0g.
Item No.
Name
Component
Size
SD7003
(S519)
LB Agar
Usage: 40.0g/L.
250 g
3
LB Broth Top Agar
Contains top agar. Used for maintenance and propagation of E.coli. Microbiologicaly tested.
Contents/L： tryptone 10.0g; yeast extract 5.0g; NaCl 10.0g, Agar 7.0g.
Item No.
Name
Component
Size
SD7004
LB-Top Agar
Usage: 32.0g/L.
LB Lennox Broth
Used for maintenance and propagation of E.coli. Microbiologicaly tested.
Contents/L：tryptone 10.0g; yeast extract 5.0g; NaCl 5.0g.
250 g
Item No.
Name
Component
Size
SD7005
(S516)
LB Lennox
Usage: 20.0g/L.
250 g
LB Lennox Agar
Contents/L： tryptone 10.0g; yeast extract 5.0g; NaCl 5.0g, agar 15.0g.
Item No.
Name
Component
Size
SD7006
LB Lennox Agar
Usage: 35.0g/L.
250 g
M9 Medium Broth
Used for propagation of E.coli and plasimid amplification. Complete media require one or more additional salts
and carbon source.
Contents/L： Na2HPO4 6.8g; KH2PO4 3.0g; NaCl 0.5g; NH4Cl 1.0g.
Item No.
Name
Component
Size
SD7024
M9 Broth
Usage: 11.3g/L.
250 g
M9CA Medium Broth
M9CA Broth is a minimal medium, based on the M9 Minimal Salts, which supports the growth of recombinant
strains of E.coli.
Contents/L： Casamino Acid 2.0g; Na2HPO4 6.8g; KH2PO4 3.0g; NH4Cl 1.0g; NaCl 0.5g.
Item No.
Name
Component
Size
SD7025
M9CA Broth
Usage: 13.3g/L.
250 g
M63 Medium Broth
Application: Propagation of E.coli and plasimid amplification. A complete media require one or more additional salts and
corbon source.
Contents/L： (NH4)2SO4 2.0g; KH2PO4 13.6g; FeSO4•7H2O 0.5mg.
pH@25 : 6.8-7.2
Additional supplements to be added after autoclaving:
10ml of sterile 20% solution of glycerol;
1ml of sterile 1M MgSO4 solution.
The above solutions should be sterilized separately by filtration before addition to the broth.
Method of preparation:
1 Suspend 15.6g of M63 Medium broth in 1L of purified water.
2 Allow the powder to dissolve. Heat and stir gently if required. Adjust pH to 7.0 with KOH.
3 Autoclave at 121 for 15 minutes.
4 Allow the solution to cool to 50 to below before adding antibiotics or additional nutritional supplements.
Item No.
Name
Component
Size
SD7033
M63 Broth
Usage: 15.6g/L.
250 g
4
NZCYM Broth
NZCYM, NZYM and NZM medium formulations are used to propagate bacteriophage, lambda. (Blattner, F, et al
(1977) Science 196, 161)For optimal binding of phage to cells, add 0.2% maltose to medium to induce the
lambda receptor (LamB) on the host cell. Maltose induction of the lambda receptor is not recommended when
making liquid lysate phage stocks because liberated phage will bind to membrane fragments containing a high
concentration of phage receptors. SuperBroth is an extremely rich medium for obtaining high yields of lambda
bacteriaphage in liquid lysates. (Brotstein, D et al (1975) J. Mol. Biol. 91, 439)
Contents /L：NZ-Amine A 10.0g; Casamino Acid 1.0g; Yeast Extract 5.0g; MgSO4×7H2O 2.0g; NaCl 5.0g.
Item No.
Name
Component
Size
SD7010
NZCYM Broth
Usage: 23.0g/L.
100 g
NZCYM Broth Agar
Contents/L：NZ-Amine A 10.0g; Casamino Acid 1.0g; Yeast Extract 5.0g; MgSO4×7H2O 2.0g; NaCl 5.0g; Agar
15.0g
Item No.
Name
Component
Size
SD7011
NZCYM Agar
Usage: 38.0g/L.
100 g
NZM Broth
Recommended for the replication and maintenance of lambda phage (NZCYM w/o casamino acids & yeast extract).
Contents/L：NZ-Amine 10.0g; MgSO4×7H2O 2.0g; NaCl 5.0g.
Item No.
Name
Component
Size
SD7014
NZM Broth
Usage: 17.0g/L.
100 g
NZM Broth Agar
Contents/L：NZ-Amine 10.0g; MgSO4×7H2O 2.0g; NaCl 5.0g; Agar 15.0g.
Item No.
Name
Component
Size
SD7015
NZM Agar
Usage: 32.0g/L.
100 g
NZYM Broth
Recommended for the replication and maintenance of lambda phage (NZCYM w/o casamino acids).
Contents/L：NZ-Amine A 10.0g; Yeast Extract 5.0g; MgSO4×7H2O 2.0g; NaCl 5.0g.
Item No.
Name
Component
Size
SD7012
NZYM Broth
Usage: 22.0g/L.
100 g
NZYM Broth Agar
Contents/L：NZ-Amine A 10.0g; Yeast Extract 5.0g; MgSO4×7H2O 2.0g; NaCl 5.0g; Agar 15.0g.
Item No.
Name
Component
Size
SD7013
NZYM Agar
Usage: 37.0g/L
100 g
SOB Broth
SOB Broth, as a rich medium, is used for the cultivation strains of E.coli. By placing the bacterium in this rich
medium, perforations are being created in the bacterial cell wall. Thus, they are being prepared for the
introduction of foreign DNA into the cell, and to help survive this process, they are placed into this rich, isotonic
environment.
Contents/L：Tryptone 20.0g; Yeast extract 5.0g; Sodium chloride 0.5g. MgSO4×7H2O 5.0g.
pH@25℃: 7.0-7.4.
Item No.
Name
Component
Size
SD7007
SOB Broth
Usage: 30.5g/L.
250 g
SOB Broth Agar
Contents/L：Tryptone 20.0g; Yeast extract 5.0g; Sodium chloride 0.5g; MgSO4×7H2O 5.0g; Agar 15.0g.
pH@25℃: 7.0-7.4.
Item No.
Name
Component
Size
SD7008
SOB Agar
Usage: 45.5g/L.
250 g
5
SOC Broth
SOC is a special medium for incubating competent cells immediately after transformation to allow expression of
transffered resistance genes before exposing cells to selective conditions.
Contents/L：Tryptone 20.0g; Yeast Extract 5.0g; NaCl 0.5g; MgSO4×7H2 O 5.0g; D-Glucose 3.6g.
Item No.
Name
Component
Size
SD7009
SOC Broth
Usage: 34.0g/L.
250 g
Super Broth
For high yields of lambda phage.
Contents/L：Tryptone-B 35.0g; Yeast Extract 20.0g; NaCl 5.0g.
Item No.
Name
Component
Size
SD7026
Super Broth
Usage: 60.0g/L.
250 g
Super Broth Agar
Contents/L：Tryptone-B 35.0g; Yeast Extract 20.0g; NaCl 5.0g; Agar 15.0g.
Item No.
Name
Component
Size
SD7027
Super Broth
Agar
Usage: 75.0 g/L.
250 g
Super Broth Top Agar
Contents/L：Tryptone-B 35.0g; Yeast Extract 20.0g; NaCl 5.0g; Agar 7.0g.
Item No.
Name
Component
Size
SD7028
Super Broth
Top Agar
Usage: 67.0g/L.
250 g
Terrific broth
Used for the preparation of molecular genetic strains of E.coli. Supports higher density of cells to increase plasmid yield.
Contents/L：Casein digest: 12.0g; Yeast extract: 24.0g; K2HPO4: 9.4g; KH2PO4: 2.2g.
pH(47.6g/1000ml water)@25℃: 7.0-7.4
Item No.
SD7035
(DJ980)
Name
Component
Size
Usage: 47.6g/L.
250 g
Terrific broth
Tryptone Broth
Tryptone broth is a moderately-rich, general purpose medium for growing E.coli.
Contents/L：Tryptone 10.0g; NaCl 8.0g.
Item No.
Name
Component
Size
TYGPN Medium
TYGPN is a rich medium on which E coli will grow rapidly. This medium contains essential amino acids, vitamins,
and other metabolites that the cell would otherwise need to synthesize. The additional of glycerol provides a
carbon source and the sodium phosphate, dibasic and potassium nitrate help to buffer the system against
radical changes in pH due to metabolism. In this rich medium, a typical strain will double in number every 20 to
30 minutes.
Contents/L：Tryptone 20.0g; Yeast extract 10.0g; Na2HPO4 5.0g; KNO3 10.0g.
pH@25℃: 7.0-7.4.
Item No.
Name
Component
Size
SD7032
TYGPN
Usage: 45.0g/L.
250 g
6
YM Broth
YM medium is specifically formulated for the growth and expression of Agrobacterium. Use of YM Medium in
electroporation of Agrobacterium results in up to a 40-fold increase in transformation efficiency when compares
with the use of LB broth. No aggregation of Agrobacterium was observed when YM Medium was used in culturing
the bacteria for plant infection.
Contents/L：Yeast Extract 3.0g; Peptone 5.0g; D-glucose 10.0g; Malt Extract 3.0g.
Item No.
Name
Component
Size
SD7031
YM
Usage: 21.0g/L.
250 g
YM Broth Agar
Contents/L：Yeast Extract 3.0g; Peptone 5.0g; D-glucose 10.0g; Malt Extract 3.0g; Agar 15.0g.
Item No.
Name
Component
Size
SD7034
YM Agar
Usage: 36.0g/L.
250 g
YPD Broth
YPD Broth is excellent for the propagation and cultivation of yeast, such as Saccharomyces cerivisiae and other
yeast organisms. Peptone in the broth provides a rich source of carbon, nitrogen, vitamins and minerals for the
organisms. Yeast extract and glucose provide nucleotide precursors and energy sources additional vitamins.
Contents/L：Yeast Extract 10.0g; Peptone 20.0g; D-glucose 20.0g.
Item No.
Name
Component
Size
SD7022
YPD Broth
Usage: 50.0g/L.
250 g
YPD Broth Agar
Contents/L：Yeast Extract 10.0g; Peptone 20.0g; D-glucose 20.0g; Agar 15.0g.
Item No.
Name
Component
Size
SD7023
YPD Agar
Usage: 65.0g/L.
250 g
YT Broth
YT and 2×YT medium are optimized for the growth and maintance of M13 phage or other filamentous ssDNA
bacteriophages.
Contents/L：Yeast Extract 5.0g; Tryptone 8.0g; NaCl 5.0g.
Item No.
Name
Component
Size
SD7016
YT
Usage: 18.0g/L.
250 g
YT Broth Agar
Contents/L：Yeast Extract 5.0g; Tryptone 8.0g; NaCl 5.0g; Agar 15.0g.
Item No.
Name
Component
Size
SD7017
YT Agar
Usage: 33.0g/L.
250 g
YT Top Agar
Contents/L：Yeast Extract 5.0g; Tryptone 8.0g; NaCl 5.0g; Agar 7.0g.
Item No.
Name
Component
Size
SD7018
YT-Top Agar
Usage: 25.0g/L.
250 g
2xYT Medium Broth
2×YT Medium is suitable for the preparation of E.coli strain infected with M13 bacteriophage.
Contents/L：Yeast Extract 10.0g; Tryptone 16.0g; NaCl 5.0g.
Item No.
Name
Component
Size
SD7019
2´YT
Usage: 31.0g/L.
250 g
2xYT Broth Agar
Contents/L：Yeast Extract 10.0g; Tryptone 16.0g; NaCl 5.0g; Agar 15.0g.
Item No.
Name
Component
Size
SD7020
2´YT Agar
Usage: 46.0g/L.
250 g
7
2xYT Broth Top Agar
Contents/L：Yeast Extract 10.0g; Tryptone 16.0g; NaCl 5.0g; Agar 7.0g.
Item No.
Name
Component
Size
SD7021
2´YT Top Agar
Usage: 38.0g/L.
250 g
8