YOUR DNA POLYMERASES
A G U A R A N T E E D Q U A L I T Y I N A N Y A P P L I C AT I O N
| D N A P O LY M E R A S E S
DNA polymerases brochure
p.2]
p.3]
PCR Enzyme Selection Guide
Introduction
p.4]
p.7]
p.8]
p.9]
The Diamond Taq® family
Diamond & Red Diamond Taq®
Hot Diamond Taq®
HGS Diamond Taq®
p.10]
SilverStar DNA polymerase
p.11]
Ready-to-use PCR mixes
GoldStar®/HGS
p.12]
Related products
PCR Enzyme Selection Guide
Free
Sample
Page
PCR qPCR Yield Sensitivity Fidelity Specificity
Visual
Difficult
Long
recognition
template
template
Low copy
(GC & AT rich)
template
3’ end
Regular Polymerase
7
Diamond Taq®
7
Red Diamond Taq
10
SilverStar
11
11
TAQ-I021-100
***
*
++
++
++
++
*
**
3’ A
TAQ-I041-100
***
*
++
++
++
++
*
**
3’ A
ME-0074-SA
***
*
+++
+++
+
+
*
**
3’ A
GoldStar Mix
PK-0064-02SA
***
*
++
++
++
++
*
**
3’ A
Red'y Gold Mix
PK-0064-02RSA
***
*
++
++
++
++
*
**
3’ A
TAQ-I033-100
***
**
+++
+++
++
+++
***
***
3’ A
®


Hotstart Polymerase
8
Hot Diamond Taq®
9
HGS Diamond Taq
TAQ-I011-100
***
***
+++
+++
++
+++
**
**
3’ A
11
Hot GoldStar Mix
PK-0073-02SA
***
***
+++
+++
++
+++
**
**
3’ A
11
Red'y Star Mix
PK-0073-02RSA
***
***
+++
+++
++
+++
**
**
3’ A
®
* Weakly recommended
** Recommended
*** Highly recommended
+ Low
++ Medium
+++ High
++++ Very high

93
Many applications
require DNA polymerases
with the highest quality
standards in purity and
Introduction
production processes.
Diamond Taq® family
enzymes are developed
and produced according
to cGMP compliant
Polymerase Chain Reaction (PCR ) is nowadays established
as a core technology in R&D and for diagnostic purpose.
In both fields, you need a product you can rely on durably
as well as flexible services ensuring optimal nucleic acid
amplification.
processes, ensuring the
highest reproducibility,
consistency and sensitivity
in every PCR applications.
High performance DNA polymerases are purified
according to multiple purification protocols minimizing
the amount of E. coli DNA trace present in the final Taq
polymerase preparation, allowing you to work with minimal
background. All our enzymes are tested extensively for the
absence of nicking and priming activities, exonucleases
and non-specific endonucleases. l
These enzymes have
been easily validated
by top laboratories and
several major diagnostic
companies. n
Classified Cleanrooms (ISO 7/8).
IVD Validated Methods, Qualified Equipments.
Batch Records, Traceability, ISO 13485.
Partnership for all needs
First Idea
Life
Science Taq
polymerases
Feasability
Design Control
Validation
Diamond Taq®
polymerases
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Commercialization
| D N A P O LY M E R A S E S
Additional information
Documentation] Enzymes are
provided with specification sheet &
Certificate of Analysis with the QC
data released by a QC authorized
The Diamond Taq®
family
person and based on review of the
complete batch record.
Order & Shipping conditions]
Diamond Taq® family enzymes can
be ordered & shipped following two
All the Diamond Taq® enzymes have been manufactured
under fully compliant cGMP conditions, purifications and
quality controls to offer you the maximum security in your
assay.
processes:
IVD label]
kShipping on dry ice.
k Full traceability from
Diamond Taq® family enzymes are highly thermostable
polymerases produced and purified from recombinant
Escherichia coli bacteria containing the Thermus
aquaticus DNA Polymerase gene.
production, storage to
shipment of the product.
k Tracking number sent to
The expressed enzyme shows very good fidelity and
catalyzes 5’>3’ synthesis of DNA with no detectable 3’>5’
exonuclease activity. The enzyme has the “extendase”
activity allowing TA cloning. l
customer the day of the
shipment.
Research label]
k
Shipping at room temperature
EOS
(except for Hot DiamondTaq®,
Eurogentec Ordering System
on dry ice).
k
k
k
Full traceability from
Higher Sensitivity
Higher lot-to-lot consistency
¡S
trickly monitored GMP and analytical processes
ensuring lot-to-lot consistency.
k Ultra-low bioburden
¡P
roduction in a GMP-Pharma facility leading to a
guaranteed bioburden between 0 and 10 CFU/ml.
k Ultra-low residual DNA content
¡Q
C-tested ensuring less than 1 fg (0.2 copy) of
genomic E. coli DNA / Taq unit.
k Lower risk of false positive results due to residual
DNA contamination (bacterial & fungal).
production to storage
of the product.
✔
EOS
Eurogentec Ordering System
GoldStar® ] becomes
RedGoldStar ] becomes Diamond Taq® qualities
Diamond Taq®
Red Diamond
HotGoldStar ] becomes HGS Diamond Taq®
From now on GoldStar®
enzymes are also perfectly
suited for diagnostic and
stringent PCR & qPCR
applications. Manufacturing
processes are fully compliant
to the ISO13485 Medical
Device standard.
95
Diamond Taq® family enzymes processes
Diamond Taq®
Chemical modification
Inert Red Dye
Hot modification Eurogentec patent
HGS Diamond Taq®
Red Diamond Taq®
Hot Diamond Taq®
A guarantee
of quality
in any
application
Core enzyme specifications
Parameters
Specifications
Appearance2
Clear, colorless solution1
Identity (SDS-PAGE)
MW approx. 95 kDa
Volume activity
5 U/µl
Purity (SDS-PAGE)
Over 98 %
Performance test: PCR -lDNA 2
Performance test: PCR - 18 S DNA
0.5 kb fragment positive down to 5 pg
0.1 kb fragment positive down to 10 pg
2
Ribonucleases (up to 10 U, 1 h, 37 °C)
Not detectable
Endonucleases (up to 30 U, 16 h, 65 °C)
Not detectable
Additional specifications:
Exonucleases (up to 30 U, 16 h, 65 °C)
Not detectable
Parameters
Nicking activity (up to 30 U, 16 h, 65 °C)
Not detectable
For HGS Diamond Taq
HotStart activity
Specifications
®
E. coli residual DNA
Over 1 fg / Taq Unit
Bioburden2
≤ 10 CFU/ml
Stability2
36 months (at -20 °C) from date of manufacture
For Hot Diamond Taq®
None
Performance test PCR-Numb DNA
Animal-derived additives2
Red Solution for Red Diamond Taq
1
2
®
Also for buffer & MgCl2
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No detectable amplification without 95 °C activation
0.3 kb fragment positive down to 10 pg
| D N A P O LY M E R A S E S
Diamond Taq® polymerases
manufacturing processes
Diamond Taq® polymerases
in (pre)commercial assays
are performed under
Choosing a manufacturing partner early in the
assay development process is an important step
towards successful assay validation and product
commercialization. Every aspect of the manufacturing
process is monitored and documented, providing our
partners with a high level of confidence in quality and
regulatory compliance. l
controlled conditions;
in class 10 000 cleanroom
for purification (ISO 7 with
< 100 cfu/m³) and in class
100 laminar flow hoods for
fill & finish (ISO 5 in with
< 1 cfu/m³).
Final product is released
Professional
Over 25 years experience in Oligonucleotide synthesis
by our Quality Assurance
Over 25 years experience in GMP protein manufacturing
Department with a formal
CMO for bulk reagents & GMP Oligonucleotides
Certificate of Analysis.
Skilled & fully trained staff
Full batch records are
Expert chemists in synthesis & purification
maintained and can be
reviewed during customer
Quality Commitment
Organization-wide QMS implementation
Comprehensive risk analysis and mitigation
or regulatory audits. n
Classified cleanroom facility
Full compliance to
– ISO 13485
– FDA cGMP/QSR (21 CFR Part 820)
– EU IVD Directive 98/79 EC compliance
Flexibility
Custom Fill & Finish, labeling & packaging available
Assay dispensing service
Dedicated Project Manager and Technical Specialist
Proven successful and timely delivery of assay component
Eurogentec Headquarters
+32 4 372 74 00
+32 4 372 75 00
[email protected]
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97
Additional information
The red dye does not influence
the PCR reaction and has
no effect on automated or
Diamond &
Red Diamond Taq®
manual sequencing, restriction
digestions or other downstream
applications. Removing the dye
can be easily accomplished
using any standard purification
Originally
supplied
for
Research
applications,
GoldStar® & RedGoldstar Taq DNA Polymerases, are
now manufactured according to a GMP Process and have
been renamed Diamond Taq® and Red Diamond Taq®,
improving their suitability towards in vitro Diagnostic &
demanding Research applications. l
Benefits
method.
Package content
k
k
Same enzyme as the extensively validated GoldStar ®.
Customized Fill & Finish, on request Diamond Taq®
enzyme can be produced with:
¡ An activity from 5 to 200 U/µl
¡ A glycerol level from 1 to 50 %
k Visual confirmation of pipetting (inert red dye)
included in the storage buffer of Red Diamond Taq®.
kDiamond Taq® or
Red Diamond Taq® (5 U/µl)
k
10x reaction buffer
k25 mM MgCl2 solution
Diamond & Red Diamond Taq®
Process
Diamond Taq® Red Diamond Taq®
IVD process
TAQ-I020-XXX
TAQ-I040-XXX
Classical process
TAQ-I021-XXX
TAQ-I041-XXX
xxx refers to the unit amount (1000-5000-25000)
Performance tests
Smart
Ladder
Diamond
Taq®
Competitor
GMP Taq
Diamond Taq® performance is similar or higher than competitor GMP Taq®
Diamond Taq® was evaluated against competitor GMP Taq® for its ability to
amplify different amounts (from 5 to 1000 pg) of a 0.5 kb PCR template
(λ DNA). PCR products were analyzed by gel electrophoresis.
DNA (pg):
1000100 50 5 1000100 50 5
Diamond
Taq®
Eurogentec
GoldStar Taq®
DNA (pg):
1000100 50 5 1000100 50 5
Diamond Taq® and GoldStar® Taq have the same performance
Diamond Taq® was evaluated against Eurogentec’s GoldStar Taq® for its
ability to amplify different amounts (from 5 to 1000 pg) of a 0.5 kb PCR
template (λ DNA). PCR products were analyzed by gel electrophoresis.
| D N A P O LY M E R A S E S
Hot Diamond Taq®
HotStart
characteristics
preventing
non-specific
polymerisation are accomplished through a proprietary
agent, not via a chemical modification nor a blocking
antibody; this prevents primer-dimer formation and
increases the PCR yield of specific products. Hot Diamond
Taq® shows no amplification at room temperature and
gives very high yield of specific product. The enzyme
needs very short activation time (100 % activated during
the first PCR cycle) but is compatible with all existing
protocols (from 20 sec. to 15 min. at 95 °C). l
Benefits
k Universal, amplification of long & difficult templates.
kSpecific, prevents non specific polymerisation as
primer-dimer formation and increases the PCR yield
of specific products.
k Very short activation time (minimum 20 sec.)
Package content
Hot Diamond Taq®
k Hot Diamond Taq® (5 U/µl)
Process
k 10x reaction buffer
k 25 mM MgCl2 solution
Hot Diamond Taq®
IVD process
TAQ-I032-XXX
Classical process
TAQ-I033-XXX
xxx refers to the unit amount (1000-5000-25000).
Ideal for difficult and GC rich templates amplifications
HDT C1C1 C2
C2 C3
C3 C4
C4
HDT
HDT C1
C2
C3
C4
DT
DT
Performance test on a difficult template:
Hot Diamond Taq ® (HDT) & Diamond Taq ® (DT) were evaluated against
HotStart competitors (C1, 2 & 4 - Chemical modification ; C3 - Antibody)
for their ability to amplify a difficult PCR template (gDNA NUMB - 306b).
PCR products were analyzed by gel electrophoresis.
DT
Performance test on a 72 % GC rich template:
Hot Diamond Taq ® (HDT) & Diamond Taq ® (DT) were evaluated against
HotStart competitors (C1, 2 & 4 - Chemical modification ; C3 - Antibody)
for their ability to amplify a GC rich PCR template (SCO3449 - 152b).
PCR products were analyzed by gel electrophoresis.
99
HGS Diamond Taq®
Originally supplied for Research applications only,
HotGoldStar Taq DNA Polymerase is now manufactured
according to a GMP Process and has been renamed HGS
Diamond Taq®, improving its suitability towards in vitro
Diagnostic & demanding Research applications.
HGS Diamond Taq® is a chemically modified HotStart
DNA polymerase, which initially lacks all activity below
74 °C. It requires a thermal activation of 10 minutes at
95 °C to reach maximal activity. This avoids non-specific
priming at low temperature during the PCR set-up. HGS
Diamond Taq® is more heat-stable than commonly used
Taq® DNA polymerase.
DNA fragments as long as 2 kb can be efficiently amplified.
HGS Diamond Taq® DNA polymerase provides efficient
amplification of specific products without amplifying
non-specific products or primer dimers. l
HGS Diamond Taq®
HGS Diamond Taq®
Process
IVD process
TAQ-I010-XXX
Classical process
TAQ-I011-XXX
Benefits
kPrevents
non specific polymerisation
primer-dimer formation and increases the
PCR yield of specific products.
kProven performance in many applications,
same enzyme as extensively validated and
benchmarked HotGoldStar ®.
kCustomized Fill & Finish, on request HGS
Diamond Taq® enzyme can be produced
with:
¡ An activity from 5 to 50 U/µl
¡ A glycerol level from 1 to 50 %
Package content
kHGS Diamond Taq® (5 U/µl)
k10x reaction buffer
xxx refers to the unit amount (1000-5000-25000).
k25 mM MgCl2 solution
Performance tests
HGS Diamond Taq® is a chemically modified HotStart enzyme.
HGS Diamond Taq ® is a HotStart chemically modified enzyme that shows
no amplification at room temperature but gives very high yield of specific
product after a minimum of 10 minutes of enzyme activation at 95 °C.
HGS Diamond Taq®
Eurogentec
HotGoldStar
HGS Diamond Taq® and HotGoldStar® Taq performances are similar.
HGS Diamond Taq ® was evaluated against Eurogentec’s HotGoldStar (HGS)
for its ability to amplify in triplicate 5 pg of a 0.5 kb PCR template (λ DNA).
PCR products were analyzed by gel electrophoresis.
| D N A P O LY M E R A S E S
Additional information
Storage conditions]
Storage at - 20 °C is recommended.
Shipping conditions]
Shipped at - 20 °C.
Quality Control] It is specially
purified to remove traces of DNA
SilverStar
DNA polymerase
that could interfere with some
reactions and is free from all nonspecific endonucleases, nickases
and exonucleases.
Unit definition] One unit is defined
as the amount of enzyme that
incorporates 10 nmoles of dNTPs
into acid-insoluble form in
30 minutes at 72 °C.
SilverStar
is
a
high-performance
thermostable
DNA polymerase, isolated from Thermus
aquaticus. It is an extremely robust Taq DNA polymerase
that does not require optimisation and gives high yields
with minimal background consistently from one reaction
to another. It is a highly active enzyme that produces
excellent results across a wide range of applications
such as DNA Array.
The SilverStar has a 5’Ä3’ exonuclease activity and also
the extendase activity allowing TA cloning. The enzyme
lacks the 3’Ä5’ exonuclease activity. l
recombinant
Benefits
Package content
k SilverStar
DNA polymerase (5 U/µl)
k
10x reaction buffer
k
kHigh yields.
k Robust PCR (up to 10 kb).
kHighly purified Taq polymerase.
k No optimisation needed.
50 mM MgCl2
Robust amplification
SilverStar DNA polymerase
Description
Quantity
Reference
SilverStar DNA polymerase
500 Units
ME-0074-05
SilverStar dilution buffer
1 ml
ME-0000-DI
SilverStar reaction buffer
1.5 ml
ME-0000-RE
In DNA Array application, 50,000 ORFs from more than 10
different organisms have been successfully amplified.
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| D N A P O LY M E R A S E S
C u sto m Ser vic es
Enabling Kits & Reagents
C u sto m Ser vic es
Enabling Kits & Reagents
Red loading dye
Ready to use
PCR mixes
No loading buffers or
tracking dyes required.
GoldStar Mix / Red’y’Gold Mix
Samples may be added
®
directly to an agarose
2 x concentrated PCR mixes are convenient premixed solutions
that include in a single tube all common reagents needed
for PCR. Simply dilute twice the PCR mix with template,
primers and water to start your reaction. The elimination
of cumbersome preparation steps prevents contamination
risk by multiple pipetting. Mixes usage also increases
the reproducibility of PCR reactions. The Red’Y’Gold
Mix includes a colored loading buffer allowing the direct
loading of the amplicons on a gel. l
gel after PCR without the
addition of a loading buffer
or tracking dye. The red
dye migrates as a 400500 bp fragment in agarose
gel 1 % in TBE buffer. n
HotGoldStar Mix / Red’y’Star Mix
For amplifications requiring high specificity use mixes
containing the HotGoldStar Taq polymerase, which
completely lacks any activity before the activation step,
avoiding non-specific annealing at low temperature. The
Red’y’Star Mix includes a colored loading buffer allowing
the direct loading of the amplicons on a gel. l
Note: Mixes
prepared under
ISO 13485: 2003
compliant processes
(including enzymes
of the Diamond Taq®
family) are available
on request.
Please, contact
[email protected]
Benefits
kEasy-to-use, 2x concentrated, premixed MasterMixes.
kOptimized for a broad range of targets (< 3 Kb).
kHighest sensitivity and yield.
kRobust amplification of GC-rich sequences.
k Achieves the highest quality sequencing data.
kOptionally includes a red loading dye.
Ready-to-use PCR mixes
Product
Reference
Quantity
RXNs
GoldStar Mix 2x
PK-0064-02
5 x 1 ml
200
Red’y’Gold Mix 2x
PK-0064-02R
5 x 1 ml
200
HotGoldStar Mix 2x
PK-0073-02
5 x 1 ml
200
Red’y’Star Mix 2x
PK-0073-02R
5 x 1 ml
200
Kit Content
GoldStar ® Taq polymerase (1 U/25μl), dNTPs (400 μM), MgCl2 (3 mM), Buffer
HotGoldStar Taq polymerase (1 U/25μl), dNTPs (400 μM), MgCl2 (3 mM), Buffer
Red loading dye


License statments
Related products
EOS
Eurogentec Ordering System
All the reagents you may need for nucleic acid amplification are available online on:
www.eurogentec.com
via EOS (Eurogentec Ordering System)
✔
EOS
Eurogentec Ordering System
k Custom and Universal Primers and Probes
kdNTPs
k Extraction kits
k Reverse transcriptase
k Agarose and Mupid® electrophoresis apparatus
k Molecular Weight Markers
Trademarks labels
k Diamond Taq is a registered trademark of KANEKA Eurogentec S.A .
k GoldStar is a registered trademark of KANEKA Eurogentec S.A.
k Mupid is a registered trademark of Advance Co., Ltd.
k Chemically-Modified
Hot-Start Polymerase Reagents
and Kits
Use of this product is covered by applicable US
patents and corresponding patent claims outside
the US held by Roche. The purchase of this product
includes a limited, non-transferable immunity
from suit under such patent claims for using only
this amount of product for the purchaser’s own
internal research. No right under any other patent
claim, no right to perform any patented method,
and no right to perform commercial services of
any kind, including without limitation reporting the
results of purchaser's activities for a fee or other
commercial consideration, is conveyed expressly,
by implication, or by estoppel. This product is for
research use only. Additional rights for use in
non-diagnostic fields require a license from Life
Technologies. Diagnostic uses require a separate
license from Roche. Further information on
purchasing licenses may be obtained by contacting
Out-licensing at Thermo Fisher Scientific, 5791
Van Allen Way, Carlsbad California 92008;
[email protected]
®
®
®
Kaneka Eurogentec S.A.
rue Bois Saint-Jean, 5
4102 Seraing
Belgium
EGT GROUP
Toll free number: 00 800 666 00 123
Eurogentec 2017
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Kaneka Eurogentec S.A. is part of Kaneka
GRE-DNA-POLYMERASE-0617-V4
Contact:
For more information,
please contact your Sales
representative or contact us at:
[email protected]
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