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Brief
Report
The
Electrophoretic
with
By Junmi
NADELL
With
T
HE
the
technical
AND
and
assistance
Amendola
of washed
are
or patients
study
in
of
P. CREGER
of Kathryn
lymphoma3
of normal
persons
results
of a similar
Erythrocytes
Tumors
WILL1Ai’f
mobilities
carcinoma”2
red cells
ports
the
of
Malignant
ELECTROPHORETIC
with
such
Mobility
Patients
erythrocytes
reported
with
which
to be
of patients
less
than
benign
tumors.
we were
unable
those
of
This
paper
to demontrate
re-
differences.
MATERIALS
Blood
samples
were
were
determined
Mobilities
collected
in
within
AND
ethylene
24
METHODS
and
diaminetetraacetate
hours
of
collection.
determination,
1 volume
of blood
was washed
three
NaCl,
and a 0.2 per cent suspension
of red cells was
buffer
(pH 9, ionic strength
0.172).4
refrigerated
Immediately
prior
times with 10 volumes
prepared
in Michaelis
at
to
4 C.
mobility
of 1 per cent
acetate-barbital
Apparatus
The
ber
microelectrophoresis
and
Ag-AgCl
description
several
and
repair
of
chamber
from
ends
of
female
joints
male
halves
are
apparatus
electrodes.
minor
the
halves
of
of these
sealed
technical
apparatus.
electrode
the
with
size of the sintered
electrodes
are stored
and a small
amount
the general
cleaning
This
of
been
changes
Apiezon#{174}
sealed
grease
been
which
to the
and
held
detail
made
in
rectangular
previously.5
order
to
the
arms
electrode
of the
together
compartments.
steel
that
cleaning
the observation
are fused
to the
observation
with
discs
is 0.9-1.4
(instead
of 4-5.5
in situ in the electrode
compartments
of crystalline
KC1. The KC1 is changed
of the apparatus.
chamSince
facilitate
which
separate
oriented
and
join
side
oriented
in
glass
discs
are vertically
Pyrex#{174} joints
are
a laterally
described
have
The
sintered
compartments
10/30
joints
consists
has
The
chamber.
springs.
The
The
pore
The
silver-silver-chloride
which
contain
saturated
KCI
every
1-2 weeks
as part
of
).
Calculatious
The electrophoretic
is the electrophoretic
the cross-sectional
mobility
mobility,
area of the
was computed
from
the
d is the distance
traveled
chambers,
K is the specific
equation
by the
U
red
dAK/tI
where
U
in time t, A is
and
I is the current.
=
cell
conductance
Reproducibility
ble
Multiple
mobility
±5 per cent.
determinations
on
a single
red
From
the Division
of Hematology,
Department
of
of Medicine,
Palo Alto, Calif.
This invastigation
was supported
by USPHS
Grant
Submitted
Feb. 28, 1964; accepted
for publication
cell
sample
Medicine,
usually
were
University
Stanford
reproduci-
School
HE 06756-02
(HEM).
Apr. 17, 1964.
757
Bioon,
VOL.
24,
No.
6
(DECEMBER),
1964
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NADELL
758
Table
1.-Red
Cell
Mobilities
and Benign
of Patients
with
Surgical
Disorders
Number
Carcinoma
Lymphoma
Benign
surgical
Tumorous
Nontumorous
#{176}/sec/vo1t/cm.
-
disorders
in Michaelis
Mean
Carcinoma,
AND
Lymphoma
Standard
Mobility
Deviation
32
-1.11
±0.08
23
123
43
80
-1.13
-1.13
-1.13
-1.13
±0.06
±0.07
±0.06
±0.08
Buffer,
pH
9, ionic
strength
0.172
CRECER
at 25
±
0.1
C.
Procedure
and
resistance
determinations
were performed
0.1 C. Mobilities
were calculated
from an
for each
specimen.
Each
day specimens
from
patients
orders
were
included.
The
diagnoses
were
not known
mobilities.
Mobility
bath
in a constant
temperature
water
average
of 20 red cell migrations
with
benign
and malignant
disby the person
determining
the
at 25 ±
Sub/ects
Preoperative
blood
samples
were
collected
from
32 patients
with
carcinoma
and
123
patients
undergoing
elective
surgery
for nonmalignant
disorders.
No patients
in the latter
group
had febrile
or systemic
illnesses.
Twenty-three
patients
with
lymphoma
were
also
studied.
All diagnoses
of malignant
and benign
tumors
were established
histologically,
as
were
diagnoses
of nontumorous
conditions
where
appropriate.
The types of carcinoma
and numbers
of patients
were as follows:
breast,
6; bladder,
4;
colon, 4; basal cell, 3; cervix
3; ovary,
3; prostate,
2; lung,
1; lympho-epithelioma,
1;
melanoma,
1; penis,
1; stomach,
1; thymus,
1; tongue,
1.
The
types
Hodgkin’s
The
with
of
lymphoma
disease,
and
10; reticulum
123 patients
nontumorous
without
conditions.
number
of
sarcoma,
2.
malignancies
The
patients
were
consisted
types
as
of
of benign
follows:
43
with
lymphosarcoma,
benign
11;
tumors
and
80
tumors
and numbers
of patients
were
as follows:
fibrocystic
disease
of breast,
9; leiomyoma,
5; fibroadenoma
of breast,
4; polyp
of colon,
4; prostatic
hypertrophy,
4; ganglion,
3; intraductal
papilloma
of breast,
3;
thyroid
adenoma,
3; Brenner
tumor
of ovary,
1; cervical
polyp,
1; cystadenoma
of ovary,
1; endometriosis,
1; meningioma
1; osteochondroma,
1; pilonidal
cyst, 1; synovial
cyst, 1.
The types
of nontumorous
conditions
and numbers
of patients
were
as follows:
cholelithiasis,
13; hernia,
10; opthalmologic
disorders,
10; lower
urinary
tract
infection
(cystoscopy),
7; normal
tissue
biopsy,
7; orthopedic
disorders,
7; otorhinolaryngologic
disorders,
6; plastic
procedures,
5; functional
uterine
bleeding,
4; hemorrhoids,
3; varicose
veins,
2; abscess,
1; diverticulosis,
1; duodenal
ulcer,
1; gastric
ulcer,
1; peripheral
vascular disease,
1; salivary
duct stone,
1.
Rssws
It
no
can
be
seen
appreciable
cinoma,
from
differences
lymphoma,
the
results
among
and
tumorous
presented
the
red
in
cell
table
mobilities
or nontumorous
I
that
there
were
of patients
with
surgical
conditions.
benign
car-
DIscussIoN
We
that
in
were
the
subjects
not
able
electrophoretic
without
to confirm
the
mobility
of red
malignancies.
The
finding
cells
mean
of
Rottino
is lower
mobilities
and
his
in malignant
of
red
co-workers1’3
disease
cells
than
reported
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ELECTROPHORETIC
by Ilottino
for patients
We
MOBILITY
and Angers’
for
with
carcinoma
obtained
mean
0.07, in carcinoma
±
same
slowly
than
values
those
for
subjects
preparation
as
bilities
are
without
by
our
use
Rottino
similar
to
et
those
The
strength,
electrophoretic
There
In
was
in the
Esseva
difference
maligne.
tate
determinate
le
conditiones
In
erythrocytos
IN
the mobility
tvo groups
and
technic
of sample
out
who
that
our
obtained
acetate-barbital
of the technic
apparatus
momo-
buffer.*
such
as
may
account
the
mobilities
with
benign
determined.
of the
untreated
two
groups.
erythrocytes
de
e ab
appreciabile
55
is not
erythrocytos
patientes
differentia
instrumento
de
in
con
inter
microelectrophorese
utile
patients
were
123
disorders
electrophoretic
manos,
es un
of
INTERLINCUA
chirurgic
nulle
nostre
non
the
pointed
same
aspects
Rottino
malignancies.
mobilitates
constatate
gruppos.
but
Ponder7
washed,
of human
con
Esseva
le duo
and
by
Not only did
migrated
more
CONCLUSIONS
between
of
diagnosis
benigne
1.13
the
between
buffer
be
of erythrocytes
with malignant
SUMMARIO
patientes
same
using
the
in other
microelectrophoresis
tool
Ponder
reported
report.
tumors
disorders
It might
AND
mobilities
55 patients
and
0.05.
±
of
using
obtained.
no appreciable
hands
our
a useful
data
or microelectrophoresis
results
conditions
of the
of
SUMMARY
surgical
the
significantly
al.6
is/sec/volt/cm.
that
differences
field
contrasting
in
differ
tumors
1.09
disorders
± 0.06,
authors.
malignant
subjects
is so despite
method,
the
latter
differences
of
used
very
and nonsystemic
in lymphoma
-1.13
in the present
with
malignant
-
timing
the
,z/sec/volt/cm.,
-
and those
contained
red cells of patients
bilities
of -1.0
1.1
We can only suggest
for
for
without
This
of workers.
the
as
account
his co-workers”3
fail to find that
we
±
were
1.33 ± 0.05
and with
lymphoma
subjects
± 0.02,
in benign
0.08, and
buffer
to
759
ERYTHROCYTES
normal
-1.16
mobilities
-1.11
acetate-barbital
It is difficult
and
OF
ab
123
disordines
le mobilitates
lavate,
le diagnose
in
non-pretracde
malignitates
human.
REFERENCES
1. Rottino,
A., and Angers,
phoretic
mobility
of
carcinoma
cer
2. Crace,
blood
benign
0It
and
Res.
W.
should
21:1445,
J., and
cell
and
be
other
J.: The
erythrocytes
in
Can-
diseases.
1961.
Rottino, A.: The
electrophoretic
malignant
noted
electro-
that
mobility
disorders
the
red
red
gastrointestinal
tract.
Ann.
mt. Med.
58:414,
1963.
3. Rottino,
A., and Angers,
J. W.: Report
of a three-year
study
of red cell mohility
and slowing
factor
in serum
of
in
persons
of the
1962.
cell
reported
mobiities
with
lymphoma.
in the
present
comparable
to those previously
reported
from this laboratory.5
The phosphate
in the previous
study
gives mobilities
about
25 per cent faster
than those
the acetate-barbital
buffer
used in the present
study.
Blood
20:750,
paper
are
buffer
obtained
not
used
with
From www.bloodjournal.org by guest on June 18, 2017. For personal use only.
760
NADELL
4. Bull, H. B.: Physical
Biochemistry.
New
York, Wiley
and Sons, 1943, pp. 122123.
5. Nadell,
J., and Creger,
W. P.: Microelectrophoresis:
Refinement
of method
and analysis
of results
obtained
with
human
erythrocytes. Stanford
Med.
Bull. 20:142,
1962.
Judith
cine,
William
Head,
Nadell,
Stanford
P.
Division
6. Angers,
J.,
and
Rottino,
M.D.,
7.
Associate
of Hematology,
Medicine,
Stanford
Palo
A.: The
CRECER
electro-
phoretic
mobility
of red blood
cells
of normal
human
beings.
Blood 17:119,
1961.
Ponder,
E., and Ponder,
R. V.: Electrophoretic
mobility
of red cells and their
ghosts
as observed
with improved
apparatus.
J. Exper.
Biol. 32:175,
1955.
M.D.,
Research
Associate,
Department
University
School
of Medicine,
Pak
Creger,
AND
Alto,
Professor
University
Calif.
of MediAlto,
Calif.
of
Medicine,
School
of
From www.bloodjournal.org by guest on June 18, 2017. For personal use only.
1964 24: 757-760
Brief Report: The Electrophoretic Mobility of Erythrocytes of Patients with
Malignant Tumors
JUDITH NADELL, WILLIAM P. CREGER and Kathryn Amendola
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