BD Transduction Laboratories™
Technical Data Sheet
Purified Rabbit Anti-Stat1 (N-terminus)
Product Information
610119
50 µg
250 µg/ml
QC Testing: Human
Tested in Development: Chicken, Dog, Frog, Mouse, Rat
91/84 kDa
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium
azide.
Material Number:
Size:
Concentration:
Reactivity:
Target MW:
Storage Buffer:
Description
ISGF3 (interferon-stimulated gene factor 3) is the primary transcription activator induced by the binding of interferon to a specific cell surface
receptor. Functional ISGF3 is a complex of four polypeptides of 113 kDa (Stat2), 91 kDa (Stat1α), 84 kDa (Stat1β), and 48 kDa. The first
three polypeptides contain both SH2 and SH3 domains in response to the binding of IFNα, IFNγ, EGF, PDGF, or CSF-1 to their respective
receptors, the cytoplasmic ISGF3 subunits become tyrosine-phosphorylated and are translocated to the nucleus. This results in the formation
of an active complex with the 48 kDa protein. This complex is responsible for modulating the transcription of the interferon-stimulated genes
(ISGs). Thus, growth factors and cytokines appear to activate a common signal transduction pathway which leads to the phosphorylation and
subsequent nuclear translocation of a group of latent cytoplasmic transcription factors.
Western blot analysis of Stat1 on A431 cell lysate.
Lane 1: 1:1000, lane 2: 1:2000, lane 3: 1:4000 dilution of
anti-Stat1.
Immunofluorescent staining of human endothelial
cells.
Preparation and Storage
The polyclonal antibody was purified from antiserum by negative adsorption and affinity chromatography.
Store undiluted at -20°C.
Immunogen was from human Stat1 aa. 1-194.
Application Notes
Application
Western blot
Routinely Tested
Immunofluorescence
Tested During Development
Immunoprecipitation
Tested During Development
Immunohistochemistry
Not Recommended
Recommended Assay Procedure:
Western blot: Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml.
610119 Rev. 1
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Suggested Companion Products
Catalog Number
611447
554002
554001
Name
A431 Cell Lysate
HRP Goat Anti-Mouse Ig
FITC Goat Anti-Mouse Ig
Size
500 µg
1.0 ml
0.5 mg
Clone
(none)
(none)
Polyclonal
Product Notices
1.
2.
3.
4.
Since applications vary, each investigator should titrate the reagent to obtain optimal results.
Please refer to www.bdbiosciences.com/pharmingen/protocols for technical protocols.
Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before
discarding to avoid accumulation of potentially explosive deposits in plumbing.
Source of all serum proteins is from USDA inspected abattoirs located in the United States.
References
Chang TL, Peng X, Fu XY. Interleukin-4 mediates cell growth inhibition through activation of Stat1. J Biol Chem. 2000; 275(14):10212-10217.(Clone-specific: Gel
shift, Western blot)
Fu XY, Schindler C, Improta T, Aebersold R, Darnell JE Jr. The proteins of ISGF-3, the interferon alpha-induced transcriptional activator, define a gene family
involved in signal transduction. Proc Natl Acad Sci U S A. 1992; 89(16):7840-7843.(Biology)
Fu XY, Zhang JJ. Transcription factor p91 interacts with the epidermal growth factor receptor and mediates activation of the c-fos gene promoter. Cell. 1993;
74(6):1135-1145.(Biology)
Lemmink HH, Tuyt L, Knol G, Krikke E, Vellenga E. Identification of LIL-STAT in monocytic leukemia cells and monocytes after stimulation with interleukin-6 or
interferon gamma. Blood. 2001; 98(13):3849-3852.(Clone-specific: Immunoprecipitation, Western blot)
Su WC, Kitagawa M, Xue N, et al. Activation of Stat1 by mutant fibroblast growth-factor receptor in thanatophoric dysplasia type II dwarfism. Nature. 1997;
386(6622):288-292.(Clone-specific: Immunohistochemistry)
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