Principal Investigator/Program Director (Last, First, Middle):
BIOGRAPHICAL SKETCH
Provide the following information for the key personnel and other significant contributors in the order listed on Form Page 2.
Follow this format for each person. DO NOT EXCEED FOUR PAGES.
NAME
POSITION TITLE
Rakasz, Eva Gizella
Associate Scientist
eRA COMMONS USER NAME
ERAKASZ
EDUCATION/TRAINING (Begin with baccalaureate or other initial professional education, such as nursing, and include postdoctoral training.)
INSTITUTION AND LOCATION
DEGREE
(if applicable)
YEAR(s)
FIELD OF STUDY
Eotvos Lorand University of Sciences, Budapest, HU
Eotvos Lorand University of Sciences, Budapest, HU
National Institute of Rheumatology, Budapest, HU
Research Associate, University of Iowa, Iowa City, IA
B.S.
Ph.D.
Postdoc.
Postdoc.
06/1983
09/1987
01/1991.
04/1994.
Biology
Biology
Immunology
Mucosal Immunology
A. Personal Statement
Briefly describe why your experience and qualifications make you particularly well-suited for your role
(e.g., PD/PI, mentor, participating faculty) in the project that is the subject of the application.
I have been the leader of the Immunology Core Laboratory at the Wisconsin National Primate Research Center
for more than 12 years. I have ample experience conducting biomedical research in Indian Rhesus macaques and
supporting the research endeavors of multiple academic laboratories at the same time. I have more than 27 years
experience in sterile in vitro assays ranging from cell line establishment to viral suppression assays, and more
than 15 years using flow cytometric analysis.
B. Positions and Honors:
Positions
1983-1990.
1991-1994.
1994-1998.
1998-2004.
2004-
Research Assistant, National Institute of Oncology, Budapest, Hungary
Research Associate, National Institute of Rheumatology, Budapest Hungary
Research Associate, University of Iowa, Iowa City, IA
Assist. Scientist, WNPRC-University of Wisconsin-Madison, WI
Assoc. Scientist, WNPRC-University of Wisconsin-Madison, WI
Honors
1993-1996
Executive committee member of the Hungarian Inflammatory Research Foundation
2002. GLR-CFAR Developmental Award, 2006. AmfAR Research Award, 2007. University of WisconsinMadison Academic Staff Professional Development Award
2004. Sexually Transmitted Infections and Topical Microbicide Clinical Res. Center Special Emphasis Panel
Member; 2007. Scientific Committee member of the 26th Annual Symposium for Non-human Primate Models of
AIDS; 2009. Reviewer for the University of Madison ICTR pilot project proposals.
C. Selected peer-reviewed publications in chronological order (out of 48)
de Andres, B., Rakasz, E., Hagen, M., McCormik, M. L., Mueller, A. L., Elliot, D., Metwali, A., Sandor, M.,
Britigan, B.E., Weinstock, J.V., Lynch, R.G. Lack of Fc-e receptors on murine eosinophils: Implications for the
functional significance of elevated IgE and eosinophils in parasitic infections. Blood 1997. 89 (10): 3826-3836.
Rakasz, E., Blum, A.M., Metwali, A., Elliott, D., Jie, L., Qadir, K., Ballas, Z., Lynch, R.G., Weinstock, J.V.
PHS 398/2590 (Rev. 09/04, Reissued 4/2006)
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Biographical Sketch Format Page
Principal Investigator/Program Director (Last, First, Middle):
Origin and regulation of IFN-g production within granulomas of IL-4 deficient and control mice. J. Immunol.
1998. 160: 4994-4999.
MacDougall, A. V., Enders, P., Hatfield, G., Pauza, C. D., and Rakasz, E. Vg2 T cell receptor repertoire overlap
in different anatomical compartments of healthy, unrelated rhesus macaques. J. Immunol. 2001. (166): 22962302.
Reynolds, M., Rakasz, E., Skinner, P. J., Swenson-White, C., Abel, K., Ma, M., Compton, L., Napoe, G.,
Wilson, N., Miller, C.J., Haase, A.T., Watkins, D.I. The CD8+T-Lymphocyte Response to Major
Immunodominant Epitopes after Vaginal Exposure to SIV: Too late and Too Little. J. Virol. (selected for JVI
Spotlight) 2005. 79 (14): 9228-9735
Loffredo, J.T., Rakasz, E.G., Giraldo, J.P., Spencer, S.P., Grafton, K.K., Martin, S. R., Napoe, S. G., Yant, L.
J., Wilson, N.A., Watkins, D. I. Tat28-35SL8 specific CD8+ T lymphocytes are more efficacious than Gag181189CM9-specific CD8+T lymphocytes at suppressing SIV replication in a functional in vitro assay. J. Virol.
2005. 79(23): 14986-14991.
Kaizu, M., Weiler, A.M., Weisgrau, K.L., Vielhuber, K., May, G., Piaskowski, S.M., Furlottt, J., Maness, N.J.,
Friedrich, T.C., Loffredo, J.T., Usborne, A., E.G. Rakasz. Repeated intravaginal inoculation with cellassociated SIV results in persistent infection of non-human primates. J. Infect. Dis. 2006. 194(7): 912-916.
Loffredo, J.T., Burwitz, B.J., Rakasz, E.G., Spencer, S.P., Stephany, J.J., Bean, A.T., Giraldo, J.P., Sarah R.
Martin, Reed, J., Piaskowski, S.M., Furlott, J., Weisgrau, K., Rodrigues, D.S., Napoé, S.G., Wilson, N.A.
Kallas, E.G., and D.I. Watkins. SIV-specific CD8+ T cell antiviral efficacy is unrelated to epitope specificity
and abrogated by viral escape. J. Virol. 2007. 81(6):2624-2634.
Sacha, J.B., Chung, C., Rakasz, E.G., Loffredo, J.T., Bean, A.T., Spencer, S.P., Jonas, A.K., Lee, W., Burwitz,
B., Stephany, J., Napoe, G., Adnan, S., Hoji, A., Wilson, N., Friedrich, T.C., Liffson, J.L., Yang, O.O., and D.I.
Watkins. Gag-specific CD8+ T lymphocytes Recognize infected cells before AIDS virus integration and protein
expression. J. Immunol. 2007. 178: 2746-2754.
Maness, N.J., Valentine, L.E., May G.E., Reed, J., Piaskowski, S.M., Soma, T., Furlott, J., Rakasz, E.G.,
Friedrich, T.C., Hughes, A.L., Sidney, J., Sette, A., Wilson, N.A., D.I. Watkins. CD8+ T lymphocytes against an
immunodominant, cryptic epitope select for escape variants in AIDS virus infection. J. Exp. Med. 2007.
204(11): 2505-2512.
Weiler, A.M., Li, Q., Duan, L., Kaizu, M., Weisgrau, K.L., Friedrich, T.C., Reynolds, M.R., Haase, A.T., and
E.G. Rakasz . Genital ulcers facilitate rapid viral entry and dissemination following intravaginal inoculation
with cell-associated SIVmac239. J. Virol. 2008. 82(8): 4154-4158.
Reynolds, M.R., Weiler, A.M., Weisgrau, K.L., Piaskowski, S.M., Furlott, J.R., Weinfurter, J. T., Kaizu, M.,
Soma, T., Leon, E.J., Mac Nair, C., Gostick, E., Phung, P., Chappey C., Schweighardt, B., Wrin, T., Musani,
S.K., Price, D.A. , Friedrich, T.C., Rakasz, E.G., Wilson, N.A., McDermott, A.B., Boyle, R., Allison, D.B.,
Burton, D.R., Koff, W.C., Watkins, D.I. Macaques vaccinated with live-attenuated SIV control replication of
heterologous virus. J. Exp. Med. 2008. 205(11): 2537-2550.
Hessell, A.J., Rakasz, E.G., Poignard, P., Hangartner, L., Landucci, G., Forthal, D.N., Koff, W.C., Watkins,
D.I., and D. R. Burton. Broadly Neutralizing Human Anti-HIV Antibody 2G12 is Effective in Protection
Against Mucosal SHIV Challenge Even at Low Serum Neutralizing Titers. PloS Pathogen. 2009. 5(5):e1000433.
Hessell, A.J., Rakasz, E.G., Tehrani, D.M., Huber, M., Weisgrau, K.L., Landucci, G., Forthal, D.N., Koff,
W.C., Poignard, P., Watkins, D.I., and Burton, D.R. Broadly Neutralizing Monoclonal Antibodies 2F5 and
4E10, Directed Against the Human Immunodeficiency Virus Type 1 (HIV-1) gp41 Membrane Proximal
External Region (MPER), Protect Against SHIVBa-L Mucosal Challenge. J Virol. 2010. 84(3): 1302-1313.
Friedrich, T.C., Piaskowski, S.M., Furlott, J.R., S.M., Leon, E.J., Maness, N.J., Weisgrau, K.L., Weinfurter, J.,
Weiler, A.M., Mac Nair, C., Reynolds, M.R., Wilson, N.A., and Rakasz, E.G. High Viremia is associated with
high levels of in vivo MHC-I down-regulation in Rhesus macaques infected with SIVmac239. J. Virol. 2010.
84(10):5443-5447.
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Principal Investigator/Program Director (Last, First, Middle):
PHS 398/2590 (Rev. 09/04, Reissued 4/2006)
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