Protein A ELISA Kit, Revision 4
Material Safety Data Sheet
Section 1 – Product Identification
Supplier:
Repligen Corporation
41 Seyon Street, Building #1, Suite 100
Waltham, MA 02453
Phone: (781) 250-0111; Fax: (781) 250-0115
Product Name:
Synonyms
Catalog No(s):
Protein A ELISA kit
rProtein A ELISA kit
9000-1
Section 2 – Composition / Information on Ingredients
Component
Phosphate Buffered Saline (PBS)
Reagent A (Sodium Acetate)
Reagent A (Tween 20)
Reagent B (Protein A)
Reagent C (Biotinylated Rabbit antibody)
Reagent D (Streptavidin HRP Conjugate)
Reagent E (3,3',5,5'-Tetramethylbenzidine)
CAS
Not Applicable
127-09-3
9005-64-5
Not Applicable
Not Applicable
55965-84-9
54827-17-7
Weight %
100%
15%
0.1%
<1%
<1%
<1%
15%
Section 3 – Hazards Identification
Emergency Overview:
Phosphate Buffered Saline
Reagent A
Reagent B
Reagent C
Reagent D
Reagent E
HMIS:
NFPA:
EU Risk/Safety Phrases
Irritating to eyes and skin
CORROSIVE – Causes skin and eye burns
Non Hazardous
May be irritating to eyes and skin
May be irritating to eyes and skin
Not investigated
Health Hazard:
2
Temporary or minor injury may occur
Flammability:
0
Will not ignite at room temperature
Reactivity:
Health Hazard:
0
2
Stable
Intense or continued but not chronic exposure
could cause temporary incapacitation or possible
residual injury
Fire:
0
Flash point between 38°C/100°F and 93°C/200°F
Instability:
0
Normally stable. Not reactive with water
R22:
Harmful if swallowed
R36:
Irritating to eyes
R37:
Irritating to respiratory system
R38:
Irritating to skin
S36/37/39
Wear suitable protective clothing, gloves, face/eye
protection
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Protein A ELISA Kit, Revision 4
Material Safety Data Sheet
Section 4 – First Aid Measures
Phosphate Buffered Saline
EYES: In case of eye contact, flush eyes with copious amounts of water
SKIN: In case of contact, wash with soap and copious amounts of water
LUNGS: If inhaled, move to fresh air. If breathing is difficult, give oxygen
IF SWALLOWED: Wash out mouth and consult a physician.
Reagent A
EYES: Immediately flush eyes with plenty of water for at least 15
minutes remove contact lenses if possible, seek medical attention.
SKIN: Immediately flush skin with plenty of water, remove
contaminated clothing. Seek medical advice
LUNGS: If inhaled, move to fresh air. If breathing is difficult, give oxygen
IF SWALLOWED: Seed medical advice. Do not induce vomiting.
Reagent B
EYES: In case of eye contact, flush eyes with copious amounts of water
SKIN: In case of contact, wash with soap and copious amounts of water
LUNGS: If inhaled, move to fresh air. If breathing is difficult, give oxygen
Reagent C
EYES: In case of eye contact, flush eyes with copious amounts of water
SKIN: In case of contact, wash with soap and copious amounts of water
LUNGS: If inhaled, move to fresh air. If breathing is difficult, give oxygen
Reagent D
EYES: In case of eye contact, flush eyes with copious amounts of water
SKIN: In case of contact, wash with soap and copious amounts of water
LUNGS: If inhaled, move to fresh air. If breathing is difficult, give oxygen
Reagent E
EYES: In case of eye contact, flush eyes with copious amounts of water
SKIN: In case of contact, wash with soap and copious amounts of water
LUNGS: If inhaled, move to fresh air. If breathing is difficult, give oxygen
IF SWALLOWED: Induce vomiting and seek medical advice.
Section 5 – Fire Fighting Measures
Reagent A
WARNING: Containers may burst or explode if heated. Move containers
away from heat or fire (if this can be done without risk)
FIRE EXTINGUISHING MEDIA: Water, water spray, foam, dry powder,
CO2.
Phosphate Buffered Saline
Reagents B, C, D, E
Wear self contained breathing apparatus and protective suit.
FIRE EXTINGUISHING MEDIA: Water, water spray, foam, dry powder,
CO2.
Section 6 – Steps to be Taken in the Event of a Spill or Discharge:
Applies to all kit components: (Phosphate Buffered Saline, Reagents A, B, C, D, E)
Personal Protection
Wear lab coat, gloves and eye protection. Avoid contact with spilled
material. Avoid breathing vapor or mist/dust. Provide adequate
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Protein A ELISA Kit, Revision 4
Material Safety Data Sheet
Disposal procedure:
ventilation. Eliminate any sparks, flames or ignition sources from the
spill area.
Do not allow spilled material to enter soil, waterways or drains. Do not
allow spilled material to accumulate in confined areas.
Control spillage with non-flammable absorbent materials such as sand,
earth or vermiculite. Contact licensed waste disposal contractor for
proper disposal.
Do not dispose of in drains. Follow all applicable federal, state, and local
environmental regulations.
Section 7 – Handling and Storage
Applies to all kit components: (Phosphate Buffered Saline, Reagents A, B, C, D, E)
General Precautions
Ventilation:
Respiratory Protection:
Eye/skin Protection
Storage:
Use appropriate personal protective equipment, and observe good
laboratory hygiene (see section 8).
Keep and use only in a well ventilated area
Not required if ventilation is adequate.
Standard safe laboratory practices recommended. Wear lab coat, gloves
and eye protection at all times while handling.
Keep containers closed. Keep away from flames, sparks or ignition
sources.
Store at 2-8°C/36-46°F for maximum shelf life (does not impact safety)
Section 8 – Exposure Controls/Personal Protection
Applies to all kit components: (Phosphate Buffered Saline, Reagents A, B, C, D, E)
General
Keep away from heat, sparks and flame. Avoid exposure.
Do not breathe vapor or mist. Avoid contact with eyes, skin and
clothing. Use only with adequate ventilation. Wash thoroughly after
handling
Personal protective equipment Standard laboratory practices recommended. Personal protective
equipment should be selected to provide adequate protection based
upon the procedures being performed.
Wear laboratory coat, gloves and safety glasses when handling.
Respiratory protection not required if ventilation is adequate.
Clean any exposed skin after handling, before leaving the working area,
and before eating, smoking or using the lavatory.
Clean any contaminated clothing before re-use.
Exposure limits:
Component
Phosphate Buffered Saline (PBS)
Reagent A (Tween 20)
Reagent A (Sodium Acetate)
Reagent B (Protein A)
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Exposure Limits
Not established
Not established
Not established
Not established
Protein A ELISA Kit, Revision 4
Material Safety Data Sheet
Reagent C (Biotinylated Rabbit antibody)
Reagent D (Streptavidin HRP Conjugate)
Reagent E (3,3',5,5'-Tetramethylbenzidine)
Not established
Not established
Not established
Section 9 – Physical and Chemical Properties
Phosphate Buffered Saline
Reagent A
Reagents B, C, D
Reagent E
Dry powder. Soluble in water. Neutral pH when prepared
Colorless liquid, acidic pH. Strong vinegar-like odor. Soluble in water
Colorless liquid. Neutral pH. Will not burn or ignite. Soluble in water.
Colorless liquid. Neutral pH. Will not burn or ignite. Soluble in water.
Colorless liquid. Neutral pH. Will not burn or ignite. Soluble in water.
Clear to light yellow liquid. Soluble in water.
Section 10 – Stability and Reactivity
Phosphate Buffered Saline (PBS)
Reagent A (Sodium Acetate/Tween 20)
Reagent B (Protein A)
Reagent C (Biotinylated Rabbit antibody)
Reagent D (Streptavidin HRP Conjugate)
Reagent E (3,3',5,5'-Tetramethylbenzidine)
Stable. Hygroscopic. Incompatible with strong oxidizing
agents and acids.
Stable under normal operating conditions. Avoid heat,
flames and sparks. Incompatible with strong oxidizing
agents, peroxides, acid, caustics, glycol. May be
corrosive to metals.
Stable under normal operating conditions
Stable. Incompatible with strong oxidizing agents.
Stable under normal operating conditions
Stable under normal operating conditions
Section 11 – Toxicological Information
The kit does not contain any known carcinogens
Phosphate Buffered Saline (PBS)
Reagent A (Tween 20)
Reagent A (Sodium Acetate)
Reagent B
Reagent C (Biotinylated Rabbit antibody)
Reagent D (Streptavidin HRP Conjugate)
Reagent E (3,3',5,5'-Tetramethylbenzidine)
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May be harmful by inhalation, ingestion, or skin
absorption. Causes eye and skin irritation. Irritating to
mucous membranes and upper respiratory tract.
Ingestion of large quantities can cause weakness, GI
irritation and circulatory disturbance.
LD50 oral (rat) 40,554 mg/kg
LD50 oral (rat) 3530 mg/kg
LC50 Inhalation (rat) >30gm/m3
No known significant effects. May be skin and/or eye
irritant.
May be harmful is inhaled, swallowed or absorbed
through skin. May cause irritation of eyes, skin and
respiratory tract.
May cause skin and eye irritation and skin sensitization
in susceptible persons.
Toxicological properties have not been investigated
Protein A ELISA Kit, Revision 4
Material Safety Data Sheet
Section 12 – Ecological Information
Phosphate Buffered Saline (PBS)
Reagent A
Reagent B
Reagent C
Reagent D
Reagent E
No data available
Hazardous to aquatic life. Harmful to aquatic organisms
No known hazards
No data available
No data available
No data available
Section 13 – Disposal Considerations
Dispose of in accordance with all applicable federal, state, and local environmental regulations. Do not
allow spilled material to enter soil, waterways or drains. See also sections 6, 7 and 8.
Section 14 – Transport Information
Not regulated.
Section 15 – Regulatory Information
EU Risk Phrases:
R36/37/38:
R22
S36/37/39:
Irritating to eyes, respiratory system and skin
Harmful if swallowed
Wear suitable protective clothing, gloves and face/eye
protection
Section 16 – Other Information
The material published in this Material Safety Data Sheet has been compiled from our experience and
data presented in various publicly available publications. It is the user's responsibility to determine the
suitability of this information for the adoption of necessary safety precautions.
Repligen makes no warranty or representation about the accuracy or completeness nor fitness for
purpose of the information contained herein.
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