MONDAY 30 MARCH
POSTERSRELATEDTOCARBOHYDRATES
Mon-C-36A
Mon-C-37A
GLYCOSAMZNOGLYCANS OF G U p E A - P I G OVARY. M.Clinton*,
W.F.Long ,F.B.Williamson ,J.S.M.H$cchinson+ and
B.Seddoq+, Depts. of Biochemistry and Developmental
Biology ,University of Aberdeen,Aberdeen AE39 lAS U.K.
GLUCANS FROM THE CELL WALLS OF A g a r i c u s b i s porus FRUITING BODIES.
C. G a r c i a Mendoza and M. Novaes-Ledieu.
I n s t i t u t o Vaime r e r r i i n " d e E l i c r o b i o l o g i a ,
C.S.I.C.,
J o a q u i n C o s t a 32, bladrid G ( S p a i n ) .
The i n c o r p o r a t i o n of&] s u l p h a t e i n t o f o l l i c l e s and
corpora l u t e a removed from o v a r i e s e x c i s e d a t
s p e c i f i c t i m e s during t h e guinea-pig oestrous c y c l e
w a s s t u d i e d . Glycosaminoglycans(GAGsfwerei s o l a t e d
from these f r a c t i o n s , and i n c o r p o r a t i o n of radioi s o t o p e i n t o GAG species q u a n t i f i e d by s e l e c t i v e
p r e c i p i t a t i o n and s p e c i f i c chemical and enzymic
degradations. Maximal i n c o r p o r a t i o n of r a d i o i s o tope, per u n i t mass p r o t e i n , i n t o l u t e a l and f o l l i c u l a r c h o n d r o i t i n , dermatan and heparan s u l p h a t e s
occurred between days 2 and 5 , and days 13 and 16
a f t e r o v u l a t i o n , during t h e t i m e s of maximal l u t e a l
and f o l l i c u l a r growth r e s p e c t i v e l y . A small i n c r e a s e
i n i n c o r p o r a t i o n during days 3 and 6 i n f o l l i c u l a r
t i s s u e suggests t h a t f o l l i c u l a r growth a l s o occurs
a t t h i s t i m e . This study r e p o r t s t h e f i r s t analyti c a l i n v e s t i g a t i o n of GAGS An s e p a r a t e d l u t e a l
and f o l l i c u l a r t i s s u e taken from a s p e c i e s w i t h a
spontaneous l u t e a l phase.
T h r e e d i f f e r e n t g l u c a n s have been i s o l a t e d
from p u r i f i e d w a l l s o f s a r i c u s b i s p o r u s frg
t i n g b o d i e s : a ) a n a l k a l i - s o l u b l e , water i n s o l u b l e g l u c a n , b) a n a l k a l i - s o l u b l e , water
s o l u b l e g l u c a n , and c ) a n a l k a l i - r e s i s t a n t
glucan.
The s t r u c t u r e o f g l u c a n s , major components
o f t h e s e c e l l w a l l s , was i n v e s t i g a t e d by c h e
mica1 and enzymic s t u d i e s , and i n f r a r e d s p e c
t r o p h o t o m e t r y . KOH-soluble g l u c a n f r a c t i o n
c o n s i s t s of a n 1,3 l i n k e d d - g l u c a n and a p
(1-6)(1-3) g l u c a n , w h e r e a s t h e KOH-resistant
.-'ucan is an (1-3)(1-6) l i n k e d (3-glucan.
The above r e s u l t s on t h e s t r u c t u r e of t h e s e
g l u c a n s p r o v i d e a more e x t e n s i v e i n f o r m a t i o n
on t h e c h e m i s t r y and a r c h i t e c t u r e of a g y e g c
t e d hyphal c e l l walls.
Mon-C-38A
Mon-C-39A
TAE ACTION OF ISOAMYLASE ON FUlRIDFJLN STARCH.
COMPARISON BETWEEN GLUCOSE AND FAlTY A C I D S AS
ENERGY PRODUCING SUBSTRATES FOR THE LUNG.
R. P a r r i l l a , J . A . Martinez Izquierdo and M.S.Ayuso
I n s t . G. Maranon, C.S.I.C.,
Madrid and School of
Medicine, University of Extremadura,Badajoz, Spain.
The main substrates u t i l i z e d b y ' t h e lung t i s s u e
are glucose and f a t t y a c i d s . I n o r d e r t o determine
which of them i s t h e main energy producing s u b s t r a t e , w e have s t u d i e d their e f f e c t on the energy
l e v e l of the lung by using an isolated r a t lung
c e l l p r e p a r a t i o n as experimental model. W e have
found t h a t o l e a t e , when p r e s e n t i n t h e incubation
medium, although it i s s i g n i f i c a n t l y oxidized,
produces a d r a s t i c decrease i n the ATP l e v e l s .
This e f f e c t i s accompanied by a s u b s t a n t i a l i n h i b i t i o n of b i o s y n t h e t i c p r o c e s s e s , such as p r o t e i n
s y n t h e s i s . Glucose, when added t o g e t h e r with
oleate, w a s able t o restore both p r o t e i n s y n t h e s i s
rate and ATP l e v e l s t o normal v a l u e s , s u g g e s t i n g
that t h e g l u c o s e / f a t t y f a c t o r f o r the r e g u l a t i o n
of t h e b i o s y n t h e t i c processes and t h a t glucose is
t h e main energy producing s u b s t r a t e u t i l i z e d by
the lung t i s s u e .
Pamela Brunswick and D.J. Manners, Department of
Brewing and B i o l o g i c a l Sciences, Heriot-Watt
University, Edinburgh.
Floridean s t a r c h from D i l s e a e d u l i s is an aglucan which i s i n t e r m e d i a t e i n c e r t a i n p r o p e r t i e s
Incubation of
between glycogen and amylopectin.
Floridean s t a r c h with isoamylase from a Cytophap,a sp. caused complete debranching. This rea c t i o n gave information on t h e average chain
The r e s u l t s show a
length and chain p r o f i l e .
c l o s e r resemblance t o p l a n t , fungal and protozoal "amylopectin" than t o "animal glycogens".
The p u r i f i e d isoamylase had a molecular weight of
37,000
38,000 by SDS e l e c t r o p h o r e s i s and
gel-filtration;
t h i s value is about one-thirdof
t h a t reported i n the l i t er at u r e.
-
Mon-C-40A
Mon-C-41A
STRUCTURAL STUDIES OF AN ACIDIC POLY-
EFFECT OF EI-WKIL CELL WALL wLYsAcwmx SYNTWSIS I N W I E YBAST. T. 8irbBs and A. P a r r l l l a , Phy
s i o l o g y Department, School of Medicine, Universit y of E x t r m a d u m , b d a j o z , Spain. The effect of
e t h a n o l on p o l y s a c h a r i d e s y n t h e s i s by Sacchammyces c e r e v i s a e var. e l l i p s o i d e u s , i s o l a t e d fmm
Sp an i sh wines has been i n v e s t i g a t e d . E t y a o l (1s
v / v ) f u l l y i n h i b i t s t h e i n c o r p o r a t i o n of
CGlucg
se i n t o cell wall p o l y s a c h a r i d e s w h i l e i n co r pomt i o n i n t o bulk s o l u b l e compounds r em ai n s unaffectad. Et h an o l was found also t o i n h i b i t p r o t e i n
s y n t h e s i s , r e a s o n why t h e i n h i b i t i o n o f manan s y ~
t h e s i s co u l d b e caused by a d e c r e a s e a v a i l a b i l i t y
of manan p m t e i n moieties since m s i g n i f i c a n t
changes i n manan s y n t h e s i s have been d e t e c t e d . A t
e t h a n o l c o n c e n t m t i o n s o f I d . a t which o m t e i n
s y n t h e s i s is a l r e a d y i n h i b i t e d , o n l y menan and
not g l u c a n s y n t h e s i s were a f f e c t e d i n d i c a t i n g t h a n
b o t h affects am mediated t h m u g h d i f f e r e n t me
chanisms.
SACCHARIDE FROM DROSERA CAPENSIS
D.
C h a n n e Gowda, G. R e u t e r and R.
Schauer
Biochemisches I n s t i t u t , Universitat K i e l ,
O l s h a u s e n s t r . 40-60, D-2300 K i e l , FRG
An a c i d i c polysaccharide w a s i s o l a t e d
from t h e mucin of Drosera capensis. A s
s t u d i e d by m e t h a n o l y s i s , h y d r o l y s i s and
m e t h y l a t i o n a n a l y s i s it consistsof a g l u curonomannan backbone t o w h i c h L - a r a b i n o -
f u r a n o s y l , D - x y l o p y r a n o s y l and D - g a l a c t o pyranosyl r e s i d u e s are attached a s endgroups of d i f f e r e n t s i d e c h a i n s .
I.
-