4bases Product Portfolio
Advanced diagnostic solutions
in pharmacogenetics and oncology
www.4bases.ch
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BRCA stands for BReast CAncer
susceptibility gene. There are two
BRCA genes - BRCA1 and BRCA2.
The BRCA genes are called “tumor suppressor” genes. When functioning normally, these
genes help the body prevent cancer. They help keep
breast, ovarian, and other types of cells from growing
and dividing too rapidly or in an uncontrolled way.
When certain changes or “mutations” in the BRCA genes
hich
occur, cells are more likely to divide and change rapidly, which
reast
can lead to developing several kinds of cancer, including breast
and ovarian cancers.
n cancer
Investigating BRCA mutational status in breast and ovarian
patients plays a double role. The first, is the identification of familiar
cancer predisposition; the second, is address therapeutic choices.
All products are
marked
Breast and Ovary
HR1 BRCA 1/2 kit
to identify BRCA somatic and germline mutations
4bases HR1 BRCA 1/2 is a clinically validated kit for the identification of
both somatic and germline mutations in BRCA 1 and BRCA 2 genes using
DNA from routinely available FFPE tissue or blood samples.
Cod.: 1-2000-1-16
Cod.: IL1-2000-1-16
4bases HR1 kit to identify BRCA somatic and germline mutations from FFPE or
frozen tissue samples. 4bases HR1 kit identifies patients with advanced ovarian
cancer who may be candidates for treatment with olaparib, a new targeted therapy
for women with advanced ovarian cancer associated with defective BRCA genes.
The analysis of both somatic and germline mutations from tissue samples (FFPE or
frozen) allows oncologist to identify and treat 8-10% more patients than the only
analysis for germline mutations.
4bases HR1 kit to identify BRCA germline mutations from blood samples. 4bases HR1
commercial kit assesses a person’s risk of developing hereditary breast or ovarian
cancer based on the detection of mutations in the BRCA1 and BRCA2 genes.
Knowing the results may help patients and their healthcare professionals either
prevent or delay the onset of cancer or detect it at an earlier, more treatable stage.
HR1 BRCA 1/2 kit works on the principal NGS instruments currently available, leaving
the choice of system to the user.
Key Features
• 267 amplicons in 3 pools • 99,6% bases covered more that 100x • DNA input required: 10 ng/reaction
• Mean amplicons lenght: 112 bp • Specificity: 100% • Sensitivity: 99,1% (based on the ClinVar-COSMIC variants)
“BRCA1 and BRCA2 are among the most frequently mutated genes in high-grade ovarian serous carcinoma, which is responsible for the vast majority
of ovarian cancer deaths. Indeed, germline and somatic mutations in HR genes occur in about 30% of patients with ovarian carcinoma, of which up
to 75% are in BRCA1 and BRCA2 genes. Patients carrying a germline or somatic BRCA1/BRCA2 mutation have been associated with a better
prognosis and a better response to platinum-based therapy.“
Source: Mafficini A et al. Oncotarget. 2016 Jan 12;7(2):1076-83
For ordering info please contact [email protected]
Breast and Ovary
Workflow
The Library generation with 4bases
Breast and Ovary Kits has the following workflow:
DNA extraction
and quantification
PCR Target enrichment
Library purification
Digestion and adapter ligation
Library purification
Quantification normalizations
and pooling
Emulsion PCR
Sequencing
Data analysis
For ordering info please contact [email protected]
Colon, Lung, Skin and Thyroid
In the last decade, the Next Generation
Sequencing (NGS) technology, allowing the
high-throughput sequencing of millions of
sequences at once, had a remarkable impact on all
re
biological sciences. It allowed the sequencing of entire
ially
genomes simultaneously within few days substantially
expanding our knowledge of biological systems.
Today, the NGS technology represent one of the
he most
uencing of
powerful and accurate tools for cost-effective sequencing
complex samples for molecular diagnostic applications.
nstruction of a
However, several NGS applications require the construction
es of enzymatic
dedicated library involving a coordinated series
NA fragments for
reactions to produce a collection of specific DNA
high-throughput sequencing. The library preparation is a key step of
NGS procedure and thus the development and improvements in the
preparation of the library can have profound impact on the final outcome of
the whole procedure.
4bases presents now the Dual Steps Targeting (D.S.T.), a new technology for
library preparation that expands the applications of NGS to the field of
pharmacogenetics and diagnostic oncology. 4bases D.S.T. technology allows the
preparation of a DNA library for Ion Torrent, Illumina Miseq.
All products are
marked
The Library generation with 4bases
Colon, Lung, Skin and Thyroid Kits is divided in two steps:
STEP 1 TARGET ENRICHMENT
Target DNA sequences are amplified and labelled using specific oligonucleotide sequences called TAILS necessary for the following
BARCODING step. Select the target enrichment kit more appropriate in the product list. Build your own panel with the Single Target Kit or
chose one of the panel we propose in the Multi Target Kits. Oligonucleotides sets in the Multi Target Kits are supplied in pre-multiplexed
tubes for an easy and rapid PCR preparation. High Fidelity Taq Polymerase for STEP 1 and STEP 2 is supplied with each target enrichment kit.
STEP 2 BARCODING
PCR products obtained from STEP 1 are used as template in a second PCR in which additional sequences such as Adaptors, Keys and
Identifiers are used for specific labelling of DNA fragments. 4Bases Adaptors, Keys and Identifiers are specifically designed for Ion
Torrent, Illumina Miseq or 454S Junior Roche platforms according to instrument characteristics. The DNA Libraries obtained are
suitable for the following steps of NGS procedures such as Emulsion PCR and Sequencing with the NGS platform selected.
PGM Barcode sets and MiSeq Index sets are part of the 4bases NGS workflow and must be used in combination with 4bases target
enrichment kits.
Set 1-8
Set 9-16
Set 17-24
Set 25-32
Cod.: 2001
Cod.: 2002
Cod.: 2003
Cod.: 2004
set A (6x4) Cod.: 3001
set B (6x4) Cod.: 3002
For ordering info please contact [email protected]
Colon, Lung, Skin and Thyroid
All products are
marked
BENKit
BENKit is a CE-IVD kit for multi cancer applications. It contains reagents for the
analysis of specific genes such as KRAS, NRAS, EGFR and BRAF in separate
tubes. Different genes can be simultaneously analysed in multiplex reactions
depending on specific purposes.
ARC-Net - Research Centre for Applied Research on Cancer - clinical
evaluation of BENKit™ Multi Cancer IVD panel: KRAS, NRAS, BRAF somatic
mutations detection in paraffin embedded colon cancers; EGFR somatic
mutations detection in paraffin embedded lung cancers.
Key Features
• Number of bases targeted: 2229
• Mean amplicon lenght (including tails): 202 bp
• Primer pools: 4
• Number of amplicons: 11
• Input DNA: 20ng/reaction
• Mean coverage (10 samples - 316 chip): 13.586x
• Bases covered at >2000x: 99,99%
• Specificity: 100%
• Sensitivity: 99,99%
Cod.: 1-0122-1-16
Colon
KRAS/NRAS
KRAS/NRAS/BRAF
CRC NGS IVD PANEL
CRC NGS IVD PANEL
Cod.: 1-1000-1-16
Cod.: 1-1100-1-16
For ordering info please contact [email protected]
Cod.: IL1-1100-1-16
Colon, Lung, Skin and Thyroid
All products are
Lung
Skin
EGFR
BRAF
NGS IVD KIT exons
18, 19, 20, 21
Cod.: 1-0030-1-16
NGS IVD KIT exon 15
Cod.: IL1-0030-1-16
Cod.: 1-0020-1-16
Cod.: IL1-0020-1-16
Thyroid
Key Features
THYRO - ID
NGS IVD KIT
• 13 genes: KRAS, NRAS, HRAS,
BRAF, PIK3CA, TP53, NOTCH1,
PTEN, CDKN2A, EGFR, AKT1,
CTNNB1, hTERT
• 33 amplicons with an average
length of 183bp
Cod.: ILPRG-THY-01
DNA
Extraction
SIMPLE
PREP FFPE
SIMPLE PREP
Total Recovery
DNA extraction
From FFPE samples
DNA extraction
From low quantity tissue samples
Cod.: SP-001-48
For ordering info please contact [email protected]
Cod.: SP-002-48
marked
Colon, Lung, Skin and Thyroid
Workflow
The Library generation with 4bases Colon, Lung, Skin and Thyroid Kits has the following workflow:
1
Dna extraction
life
2
Dna quantification
®
Oubit 3.0
life
3
PCR 1: target enrichment
ment
4
Quality control of PCR products
(optional)
5
PCR 2: adapters and barcoding library
brary
6
Purification of PCR products
7
Quality control of the generated library
ProFle
x
3.
00
3
For ordering info please contact [email protected]
Stabile Suglio, Via Cantonale 18
6928 Manno - Svizzera
VAT Number CHE-338.644.087
[email protected]
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www.4bases.ch
www.dlongwood.com