Introducing C-Fern
(Ceretopteris richardii)
Examination of
the young Sporophyte Stage!!
Haploid (n)
Diploid (2n)
Sperm (n)
Mitosis and
development
Female
gametangium (n)
Gametophyte (n)
Spores (n)
Egg (n)
Fertilization
Meiosis
Clusters of
sporangia
2
New sporophyte (2n)
Zygote (2n)
3 Mitosis and
development
Mature sporophyte
Fig. 17-5-1
Key
Haploid (n)
Diploid (2n)
1
Sperm (n)
Archegonium
(female
gametangium) (n)
Gametophyte (n)
Egg (n)
Fertilization
Fig. 17-5-2
Key
Haploid (n)
Diploid (2n)
1
Sperm (n)
Female
gametangium (n)
Gametophyte (n)
Egg (n)
Fertilization
2
Zygote (2n)
Fig. 17-5-3
Key
Haploid (n)
Diploid (2n)
1
Sperm (n)
Female
gametangium (n)
Gametophyte (n)
Egg (n)
Fertilization
4
Clusters of
sporangia
2
New sporophyte (2n)
Zygote (2n)
3 Mitosis and
development
Mature sporophyte
Fig. 17-5-4
Key
Haploid (n)
Diploid (2n)
1
Sperm (n)
5 Mitosis and
Female
gametangium (n)
development
Gametophyte (n)
Spores (n)
Egg (n)
Fertilization
Meiosis
4
Clusters of
sporangia
2
New sporophyte (2n)
Zygote (2n)
3 Mitosis and
development
Mature sporophyte
Young
Sporophyte
Product of a
fertilization event!
A = young sporophyte
B = gametophyte
Fertilization in C-fern
Fertilization in C-fern!
What are all the possible fertilization events?
Complete this table in your worksheet before
examining your plate.!
P!
P!
P!
p!
p!
P!
p!
p!
P = wild allele
p = mutant allele!
PP!
WILD!
"#$%!#&!
'%()*+,%!)-!!
%''!
WILD!
Fertilization
Events in
C-Fern
PP
P
p
PP
Pp
p
P
pp
Pp
p
P
p
Examine Phenotypes of Sporophytes
•! ./#$0(%!"#!$%&'&%()*+$!#(1!2%3)21!*4%!(5$6%2!)-!
7081!#(1!$5*#(*!&,)2),4+*%&!
•! 94%(:!1%*%2$0(%!*4%!,2),)2;)(!)-!7081!#(1!$5*#(*!
&,)2),4+*%&!
•! <)!+)52!)6&%2=%1!2%&58*&!$#*34!+)52!,2%103;)(&>!
•! ?%3)21!+)52!2%&58*&!)(!*4%!6)#21@!A)5!7088!(%%1!*4%!
0(10=015#8!#(1!,))8%1!1#*#!*)!3)$,8%*%!*4%!8#6!2%,)2*@!
Wild Sporophytes: What are the phenotypes
of the gametophytes on which they grow?
Count ten randomly selected wild sporophytes
indicating the type of gametophyte it is growing on.
Do your results support your predictions?
Mutant Sporophytes: What are the phenotypes of
the gametophytes on which they grow? !
Count ten randomly selected mutant sporophytes
indicating the type of gametophyte it is growing on.
Do your results support your predictions?
Take images of the following:
•! 7081!&,)2),4+*%!)(!7081!'#$%*),4+*%!
•! 7081!&,)2),4+*%!)(!$5*#(*!'#$%*),4+*%!
•! $5*#(*!&,)2),4+*%!)(!$5*#(*!'#$%*),4+*%!
!B-!+)5!C(1!#!$5*#(*!&,)2),4+*%!)(!#!7081!
'#$%*),4+*%!@!@!@!D4#*>>>!
If you did not take pictures of
gametophytes during the last lab…
A)5!3#(!&;88!*#E%!*4%$!*)1#+!
A)5!(%%1F!!
•! 7081!#(1!$5*#(*!$#8%!'#$%*),4+*%&!
•! 7081!#(1!$5*#(*!4%2$#,42)10;3!
'#$%*),4+*%&!
Due next week:
C-Fern Lab Report
G!
H!
I!