MISEP Chem 512 - Jacobs
POGIL: Energy of ATP hydrolysis
ATP can react with water (a hydrolysis reaction) to produce ADP and a phosphate group
Complete the figure below by filling in the boxes to illustrate this reaction. Be sure to
account for conservation of all atoms.
-O—
OH
OH
hydrolysis reaction
NH,
/V7<
\d«&»
OH
-0
OH
phosphate
ADP
Concept-building questions:
1. What bond(s) is/are broken during the ATP hydrolysis reaction?
2. Is energy(absorbecPor released during the bond-breaking step(s)? Explain why.
"
**Ms
D
CJ
X^r^-)cL^
fiWStST**J<<*r*
f t the
) ATP hydrolysis reaction?
3. What bond(s) is/are formed during
WvJfvxK .~r -A
0 o
T 6-
v
A
b
4. Is energy absorbed ofCfeleaseJ1 during the bond forming step(s)? Explain why.
*-
^
?ondsj?n either the reactants^^'the^products'of the hydrolysis reaction
drawn above that are either particularly strong/stable or particularly weak/unstable?
Explain.
^
^/WJ^ML-
H^JC^ *
V
\
^
N
S-
6. Describe how yettr answers to the previous questions (#1-5) can be used to explain
why ATP hydrolysis is an exothermic reaction overall.
SynthesisKsmd Application questions:
1. Draw a potential energy diagram representing this reaction. If this reaction happened
in solution in a test tube, would you expect the test tube to feel warm or cool? Why?
Link together the macroscopic, molecular and symbolic levels of the ATP hydrolysis
reaction in your answer.
8. Although ATP hydrolysis is energetically favorable (exothermic)rit occurs very
slowly under uncatalyzed conditions. How is this observation reflected in the potential
energy diagram for the hydrolysis reaction you drew in question 7? Redraw your
diagram if necessary to reflect this new information.
, j^
~~