Datasheet BAC029-1. Pre-Filled Culture Vessel
Modified Half Fraser Broth - Dehydrated
For the Detection of Listeria spp.
Culture Vessel
The Speedy Breedy culture vessel is the disposable sterile unit that is required for each Speedy Breedy test.
Speedy Breedy provides excellent culture conditions for rapid microbial growth. The culture vessel couples
to the instrument so that internal pressure transients within (generated by microbial activity) can be
measured by Speedy Breedy over the course of a programmed time and temperature culture protocol.
Speedy Breedy can be used for a range of applications including contamination testing or microbial growth
monitoring.
Single use Speedy Breedy culture vessels are supplied sterile, either unfilled or filled with dehydrated
culture medium.
This Modified Half Fraser Broth filled vessel incorporates a ready to use culture medium in the form of
encapsulated dehydrated powder that readily dissolves in an aqueous sample (c.a. 10 minutes). Each vessel
contains sufficient medium for a working volume of 50 ml. This means that samples can be a full 50 ml
volume or smaller samples can be used and topped up to 50 ml with sterile water making this product
presentation highly versatile across a wide range of applications.
General Culture Vessel Description
The single use culture vessel totally encloses the microbial culture through the course of a timed test
protocol. The vessel provides a suitable culture enclosure for media and organisms, and is sealed,
separating it from the external environment, sensors and the operator, for safety and instrumentation
protection. Gas usage or generation due to microbial respiration is contained so that linked pressure
sensors in Speedy Breedy which houses and maintains the vessel during the test can monitor pressure
events that indicate growth. The sealed vessel avoids contamination from external sources during a culture
run.
Features

Speedy Breedy vessels manufactured from inert, impermeable plastic and rubber.

Normal working range of 5OC below ambient to 45OC.

High efficiency, non-invasive mixing system that performs three functions:
1. Homogenisation of essential nutrients and gases for optimal growth.
2. Rapid equilibration and accurate temperature maintenance.
3. Efficient gas transfer between headspace and culture medium for rapid conversion
to detectable pressure change.

Vessel pressure monitored indirectly by a sensor housed in the Speedy Breedy instrument.

Protective barrier between the vessel contents, sensor and operator.

Sample port with a removable cap including a self-sealing rubber septum with a tamper
evident seal.

Sample port allowing two distinct methods of inoculation, reagent addition or removal:
1. By removal of the cap allowing access to the vessel lumen via a 7 mm access port.
2. By piercing the cap septum with a hypodermic needle
Modified Half Fraser Broth
(Product Code BAC0029)
Modified Demi-Fraser Broth is a selective medium which is used for the detection of Listeria spp. with the
Speedy Breedy microbial respirometer, with applications in industries such as food production.
A blend of peptone, tryptone, Lab Lemco powder and yeast extract provide a highly nutritious growth
environment whilst sodium chloride maintains osmotic equilibrium and a phosphate buffer system controls
pH. Nalidixic acid, acriflavine hydrochloride and a Bactest Selectivity Mix enhance the selectivity of the
medium, improving growth of Listeria spp. organisms and suppressing Gram negative growth as well as the
growth of non-Listeria spp. Gram positive organisms such as staphylococci and enterococci.
The Modified Demi-Fraser Broth culture vessel should be used in conjunction with the Speedy Breedy
Listeria test protocol to ensure optimum growth conditions and performance.
A significant pressure event indicated by Speedy Breedy should be considered as presumptive for the
presence of Listeria spp. In the event of any positive result, confirmation by appropriate culture, serological
or molecular techniques is recommended.
Specification
Typical Formulation
Gram / Litre
Proteose Peptone
5.00
Tryptone
5.00
Lab Lemco Powder
5.00
Yeast Extract
5.00
Sodium Chloride
20.00
Disodium Hydrogen Phosphate
12.00
Potassium Dihydrogen Phosphate
1.35
Aesculin
1.00
Lithium Chloride
3.00
Nalidixic Acid
0.01
Acriflavine Hydrochloride
0.0125
Selectivity Mix
0.7185
pH 7.2 ± 0.2 @ 25°C
Typical Growth Promotion / Suppression at 36°C
Organism
Growth
Result in Speedy Breedy
Listeria monocytogenes
Turbid growth, blackening of medium
Strong pressure transient
Escherichia coli
No growth
No pressure transient
Pseudomonas aeruginosa
No growth
No pressure transient
Proteus mirabilis
No growth
No pressure transient
Staphylococcus aureus
No growth
No pressure transient
Enterococcus faecalis
No growth
No pressure transient
Typical Growth Curve – Listeria monocytogenes
Storage Conditions & Shelf Life
Store culture vessels containing dehydrated medium capsules at 10-30°C and use before the expiry date on
the label.
Precautions
Following a positive result, Speedy Breedy culture vessels will contain a rich, live culture of Listeria spp.
Speedy Breedy culture vessel design ensures operator safety. Culture vessel use, following Good Laboratory
Practice, the recommended operating procedures and the recommended disposal procedures will ensure
that operators are not exposed to infectious material.
Disposal
Used culture vessels are considered hazardous material and need to be treated as such. Health and Safety
hazard assessments should be made prior to all microbiology work including waste disposal.
For the purposes of disposal, culture vessel materials are compatible with standard procedures such as:

Autoclave 121°C @15 psi for 15 minutes.

Incineration – usually post autoclave using very high temperature incinerators.
BACTEST.......... turning microbial activity into data.
BACTEST designs,develops and markets microbial respirometry products based on its proprietary patent pending technology
platform. BACTEST’s products are proudly manufactured in England.
Speedy Breedy is a registered trademark of BACTEST Limited, St. John’s Innovation Centre, Cowley Road, Cambridge, CB4 0WS. UK.
Tel: +44 (0)1223 422313 Web: www.bactest.com E-mail: [email protected]