QUOTATION (Form A -Cycle sequencing included)
Description
Quantity
Unit Price (S$)
Proteos’ Tenants (NTU/NRP/BII)
Samples in tube
1
7.00
Samples in 96 well plate
96*
600.00 (10% discount)
ADDITIONAL INFORMATION
Usage Policy
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PO must be created and submitted together with samples.
The purchase can be made in bulk for use over multiple sessions within the same Financial Year.
For bulk purchase, PO is only needed for the first submission.
The type of purchase must be indicated clearly beforehand.
Both bulk purchase stands on its own and no combination between the two types of purchases.
The customer and details should not be changed over this period.
Purchases can be shared by multiple staff within the same laboratory.
The DNA Sequencing Service reserves the right to reject any sample.
Reloading of samples may be carried out on a case-by-case basis.
All sequencing data are kept confidential and will be deleted after 3 months.
Terms and Conditions
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Sequences will be ready for download within 2-3 working days.
Collection and Delivery: No collection and delivery of samples will be provided.
Validity and GST: Purchases must be used by 31st March of each financial year. Outstanding samples will not be
carried over to the next financial year and are non-refundable.
All prices quoted above are subjected to 7% GST.
Payment Terms: 30 days
By creating a PO, you are deemed to have agreed to our terms and conditions stated on this quotation.
Please take note that terms and conditions are subject to changes.
This is a Computer Generated Quotation. No Signature is required.
Yours Faithfully,
Jasmine Lim
Updated as of 13th May 2011
Additional Instructions:
Service is currently offered only to tenants in Proteos on a trial basis.
Samples submitted must contain primer and DNA template topped up with water (if necessary) to 6 or 12µl
for 10 or 20µl reaction volume respectively.
Please kindly indicate ‘Require Cycle Sequencing’ in the remarks column upon submission online and
deposit the samples in the respective box as to avoid confusion with purified samples.
Cycle sequencing is only carried out overnight from Monday to Thursday and will be processed the next
working day. All reactions carried out are for normal plasmids (3-4kb insert) and PCR products (<4kb),
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with annealing temperature at 52.5 C for 35 cycles. Please carry out your own cycle sequencing for
Genomic/BAC/YAC samples as these require conditions to be optimized.
For samples in 96-well plates, cycle sequencing is only carried out when there is a block available on one
of our thermocyclers. Please use only half and full-skirted 96-well plates. If you intend to submit multiple
96-well plates, please inform us in advance so we can check if blocks on thermocyclers are available. It is
assumed that samples are filled on the plates column-wise (A1-H1, A2-H2…A12-H12) for 96-well plates.
If you intend to use 384-well plates, please check with us for further instructions.
Updated as of 13th May 2011
*Correct 96-well plates must be used as sample cleanup is carried out by a robot:
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384-well plates (maximum reaction volume 8 µl inclusive of Big Dye) can also be used:
Updated as of 13th May 2011