Published on Laboratory Journal – Business Web for Users in Science and Industry (
http://www.laboratory-journal.com)
Nov. 13, 2013
Autophagosomal Membrane Located
Autophagy: The location of the site where autophagosomal membranes are created
within the cell has been the subject of two competing models for many years. Now,
a group of researchers from Osaka University led by Prof. Maho Hamasaki has
located the origin of autophagosomes within the cell and shown that neither model
is correct.
Writing in Nature (link to the article), they show that autophagosomes form at the
contact site of the endoplasmic reticulum (ER) with mitochondria.
Live cell imaging studies, using an Andor Revolution XD spinning disk confocal
microscope equipped with three Andor iXon 3 EMCCD cameras, captured
previously hidden detail. The research revealed the re-localisation of the preautophagosome/autophagosome marker ATG14 to the ER-mitochondria contact site
after starvation, along with the complementary localisation of the autophagosomeformation marker ATG5 until formation is complete.
To view videos and figures of the original article, please click on the following links:
Videos:
www.nature.com/nature/journal/v495/n7441/full/nature11910.html?WT.ec_id=NATURE20130321#videos
Figures:
www.nature.com/nature/journal/v495/n7441/full/nature11910.html?WT.ec_id=NATURE20130321#figures
Original publication:
Maho Hamasaki, Nobumichi Furuta, Atsushi Matsuda, Akiko Nezu, Akitsugu
Yamamoto, Naonobu Fujita, Hiroko Oomori, Takeshi Noda, Tokuko Haraguchi,
Yasushi Hiraoka, Atsuo Amano & Tamotsu Yoshimori. "Autophagosomes form at ERmitochondria contact sites," Nature 495, 389-93 (2013) | doi: 10.1038/nature11910.
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