Selection Chart RNA Purification
RNA purification
Whole blood
& buffy coat
Cultured cells
Standard-throughput
Tissue
High-throughput
Centrifugation
PerfectPure RNA
Blood Kit
PerfectPure RNA
Cell Kit
Fibrous tissue
Vacuum
PerfectPure RNA 96
Cell Kit
PerfectPure RNA 96
CellVac Kit
PerfectPure RNA
Tissue Kit
PerfectPure RNA
Fibrous Tissue Kit
Starting material
Whole blood and
buffy coat
Cultured cells
Cultured cells
Cultured cells
Spleen, liver, brain,
kidney, lung, small and
large intestine, etc.
Heart, skeletal muscle,
skin, esophagus, etc.
Starting amount
0.05 to 10 ml whole
blood
30 000 to
50 million cells
Up to 5 million cells
per well
Up to 5 million cells
per well
0.5 – 40 mg
0.5 – 40 mg
Yield
Up to 7 µg per ml
Up to 15 µg per
million cells
70 µg per well
70 µg per well
Up to 5 µg/mg tissue*
Up to 1 µg/mg tissue*
Processing time
Less than 35 minutes
Less than 25 minutes
96 samples in less
than 45 minutes
by centrifugation
96 samples in less
than 45 minutes
by vacuum
Less than 40 minutes
Less than 50 minutes
On-column
DNAse treatment
Yes
Yes
Yes
Yes
Yes
Key benefit
High yields of superior quality RNA from novel column-based technology without clogging or overloading
Applications
Microarray analysis;
Kit content
Real-time PCR;
cDNA synthesis;
Yes
Northern, dot and slot blotting; Ribonuclease protection assays;
In-vitro transcription; Poly-A RNA selection
Please see back page for more information on kit contents
* depending on the starting material
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Kit Content RNA Purification Kits
PerfectPure RNA Blood Kit
PerfectPure RNA 96 CellVac Kit
Sample size
0.05 – 3.0 ml
0.05 – 3.0 ml
3.0 – 10.0 ml
Sample size
Up to 5 x 106 cells / well
Preparations
10
50
50
Preparations
768
FP2302100
FP2302110
FP2302120
Ref. No.
FP2302540
PerfectPure RNA Cultured Cell Kit
Sample size
30 000 – 5 x 107 cells
Preparations
Ref. No.
PerfectPure RNA Tissue Kit
Sample size
10
50
250
250 (Base Kit)
FP2302330
FP2302340
FP2302350
FP2302360
PerfectPure RNA 96
Cell Kit 1
Preparations
Ref. No.
0.5 – 40 mg
10
50
250
FP2302400
FP2302410
FP2302420
PerfectPure RNA Fibrous Tissue Kit
PerfectPure RNA 96
Cell CS Kit 2
Sample size
Sample size
Up to 5 x 106 cells / well
Preparations
Ref. No.
1
2
Preparations
192
768
192
768
FP2302500
FP2302520
FP2302510
FP2302530
Plate Carrier Clearance >75 mm
Plate Carrier Clearance >50 mm
Ref. No.
0.5 – 40 mg
10
50
FP2302430
FP2302440
BN0529082 06/2008
Ref. No.
For customer service, call 1-800-766-7000.
To fax an order, use 1-800-926-166.
To order online: www.fishersci.com
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