DSM 12127 - Geobacter sulfurreducens (Bacteria)
© by DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH, Braunschweig, Germany
VP (Bacteria) - see also Bacterial
Geobacter
sulfurreducens
Caccavo
et
al.
1995
Name
Nomenclature Up-to-Date
DSM No.
12127
= ATCC 51573
<- B. Schink <- D. Lovley <- F. Caccavo, PCA. Ditch surface sediment; USA (7198). Type
Information
strain. Taxonomy/description (6031, 7198). (Medium 826, 35°C, anaerobic)
Medium
826
Reference(s) 6031 , 7198
Medium 826: GEOBACTER MEDIUM
NH4Cl
Na2HPO4
KCl
Na-acetate
Trace element solution (see medium 141)
Vitamin solution (see medium 141)
Selenite-tungstate solution
(see medium 385)
Resazurin
NaHCO3
Distilled water
1.50
0.60
0.10
0.82
10.00
10.00
g
g
g
g
ml
ml
1.00
0.50
2.50
980.00
ml
mg
g
ml
Dissolve ingredients (except NaHCO3), bring to a boil, cool down to room temperature while gasing
the medium with 80% N2 + 20% CO2, and add the bicarbonate. Equilibrate the medium with the gas to
reach a pH of 6.8. Dispense the medium in anaerobe tubes under N2 + CO2. After autoclaving add
fumarate from an anaerobic filter sterilized stock solution (16% w/v Na2-fumarate; 0.5 ml/10 ml
medium). Check medium pH which should be around 6.8.
see also: medium 141, medium 385
Medium 141: METHANOGENIUM MEDIUM
KCl
MgCl2 x 6 H2O
MgSO4 x 7 H2O
NH4Cl
CaCl2 x 2 H2O
K2HPO4
NaCl
Trace elements (see below)
Vitamin solution (see below)
Fe(NH4)2(SO4)2 x 7 H2O
NaHCO3
Na-acetate
Yeast extract (Difco)
Trypticase (BBL)
Resazurin
Cysteine-HCl x H2O
Na2S x 9 H2O
Distilled water
0.335
4.000
3.450
0.250
0.140
0.140
18.000
10.000
10.000
2.000
5.000
1.000
2.000
2.000
1.000
0.500
0.500
1000.000
g
g
g
g
g
g
g
ml
ml
mg
g
g
g
g
mg
g
g
ml
Prepare the medium anaerobically under 80% H2 + 20% CO2 atmosphere. For incubation use the same gas
mixture at two atmospheres of pressure. If the medium is being used without gas mixture overpressure
then adjust pH with a little hydrochloric acid. Final pH should be 7.0 for strains DSM 1497, DSM
1537, DSM 2067, DSM 2279, and DSM 2373; 6.8 for strain DSM 2095, and 6.5 for strain DSM 1498. For
DSM 2373 increase the amount of trypticase to 6 g/l.
For DSM 14042 prepare the medium under 80% N2 + 20% CO2 gas atmosphere. After sterilization add
0.05% methanol from a sterile, anoxic stock solution prepared under N2. For incubation use the same
gas mixture at two atmospheres of pressure. The final pH should be 7.0.
Trace element solution:
Nitrilotriacetic acid
MgSO4 x 7 H2O
MnSO4 x 2 H2O
NaCl
1.500
3.000
0.500
1.000
g
g
g
g
FeSO4 x 7
CoSO4 x 7
CaCl2 x 2
ZnSO4 x 7
CuSO4 x 5
KAl(SO4)2
H3BO3
Na2MoO4 x
NiCl2 x 6
Na2SeO3 x
Distilled
H2O
H2O
H2O
H2O
H2O
x 12 H2O
2 H2O
H2O
5 H2O
water
0.100
0.180
0.100
0.180
0.010
0.020
0.010
0.010
0.025
0.300
1000.000
g
g
g
g
g
g
g
g
g
mg
ml
First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Final pH 7.0
(with KOH).
Vitamin solution:
Biotin
Folic acid
Pyridoxine-HCl
Thiamine-HCl x 2 H2O
Riboflavin
Nicotinic acid
D-Ca-pantothenate
Vitamin B12
p-Aminobenzoic acid
Lipoic acid
Distilled water
2.000
2.000
10.000
5.000
5.000
5.000
5.000
0.100
5.000
5.000
1000.000
mg
mg
mg
mg
mg
mg
mg
mg
mg
mg
ml
Medium 385: DESULFOBACTERIUM CATECHOLICUM MEDIUM
Use medium 193 but lower the amount of NaCl to 1 g/l and of MgCl2 x 6 H2O to 0.4 g/l. Sodium acetate
is replaced by filter-sterilized sodium benzoate (0.37 g/l) plus catechol (0.055 g/l). Use only 2.5
g/l of sodium bicarbonate!
Add 1 ml/l selenite- and tungstate solution (0.5 g NaOH, 3 mg Na2SeO3 x 5 H2O, 4 mg Na2WO4 x 2 H2O,
1 l distilled water). Final pH of the medium should be 6.9 - 7.1.