Re-DerMAX
GFC Co., Ltd.
01 Introduction
Re-DerMAX
GFC Co., Ltd.
Introduction
01
Structure & Function of the Dermis
Thickness : 0.5~4mm, Accounts for 90% of the skin
Protects the body against external environment and
adds resilience to skin
Determines the thickness and wrinkling of skin
The basal layer, the innermost layer of the epidermis,
is nourished by diffusion from the dermis
Injury to connective tissues of the dermis is difficult to repair
Helps blood vessels to regulate body temperature
Skin cell regeneration
Introduction
Re-DerMAX
Re-Dermis + MAX = Re-DerMAX
An anti-aging ingredient that restores UV-induced DNA
damage and improves dermal density and elasticity.
01
Introduction
01
UV-induced DNA Damage
UV radiation disturbs the normal process of cell division
by inducing cells’ DNA damage
Re-DerMAX restores UV-induced DNA damage.
01
Introduction
DNA Repair System
The proteins called RecO and RecN help
quickly repair gene damages and
destructions caused by external factors such
as UV and stresses
K and L are the enzymes that influence the
expression of RecN
Re-DerMAX
RecO
RecN
The amount of K and L rapidly increases
when heat is imposed
Etc
.
Global transcriptome analysis of the heat
shock response of Bifidobacterium longum,
FEMS Microbiol Lett 271 (2007) 136~145
Increase in K and L helps produce more
RecN protein and repair DNA damage
Re-DerMAX helps produce more RecN and
RecO and reapire DNA damage
02 in vitro DNA Repair
Re-DerMAX
GFC Co., Ltd.
In vitro
DNA Repair Activity Test (in vitro)
Test Method
1.
Re-DerMAX 4.0% was treated to one
set of Fibroblasts and the other set was
untreated.
2.
Fibroblasts are exposed to UV (40mJ/
㎠)
3.
Observed after 72 hours for cell
proliferation
4.
Observed DNA damage was measured
with fluorescence microscope, after cell
staining with a nucleus dying reagent
02
02
In vitro
DNA Repair Activity Test (in vitro)
Control group
DAPI
Re-DerMAX 4.0%
DAPI
02
In vitro
DNA Repair Activity Test (in vitro)
When fibroblast cells are exposed to UV
radiation, UV ray triggers micronucleus
formation which is a sign of DNA damage
As for fibroblast cells with Re-DerMAX treated,
lesser number of micronucleus production is
shown thanks to enhanced DNA repair activity.
02 in vitro Elastase Inhibition
Re-DerMAX
GFC Co., Ltd.
02
In vitro
Elastase Inhibition
Test Method
<%>
Use the buffer solution containing Re-DerMAX
Elastase (0.4U/ml) was added to the buffer
solution and let the reaction occurs for 2-5
minutes at room temperature
Measure the level of absorbance at 410nm
Result
Does-dependent Elastase inhibition activity
Low concentration (0.25%) shows a strong
elastase inhibitory activity
<Concentration (%)>
03 in vivo Elasticity
Re-DerMAX
GFC Co., Ltd.
In vivo
03
in vivo Elasticity Improvement Test Method
Testing Agency: Korea Dermatology Research Institute
Test: Effect of Re-DerMAX 5% cream on skin elasticity
Test period: Jul. 17- Aug. 14, 2013
Test subjects: 11 (6 women in 30s and 5 women in 40s)
Testing method
The cream was applied to the forearm of subjects, 2 times a day, in morning and evening
Assessment using cutometer MPA-580
Skin elasticity was measured before subjects started testing and after 2 weeks and 4 weeks of use
03
In vivo
in vivo Elasticity Improvement
Re-DerMAX 5% cream improved skin elasticity
Re-DerMAX 5% cream improved skin elasticity
by 30% after 4 weeks of use, compared to the
starting point
03 in vivo Elasticity
Re-DerMAX
GFC Co., Ltd.
In vivo
in vivo Dermal Density Improvement
Testing Agency: Korea Dermatology Research Institute
Test: Effect of Re-DerMAX 5% cream on dermal density
Test period: Jul. 17- Aug. 14, 2013
Test subjects: 11 (6 women in 30s and 5 women in 40s)
Testing method
The cream was applied to the forearm of subjects, 2 times a day, in morning and evening
Assessment using Ultrascan UC-22 cutis
Dermal density was measured before subjects started testing and after 2 weeks and 4 weeks of use
03
03
In vivo
<Placebo>
<After 2 weeks>
<After 4 weeks>
in vivo Dermal Density Improvement
Woman at age 35 (No. 734)
Dermal density has been improved by 44.4% after 2 weeks and 55.6% after 4 weeks of use
Density of subcutaneous tissues has also been improved, as well as dermal
03
In vivo
<Placebo>
<After 2 weeks>
<After 4 weeks>
in vivo Dermal Density Improvement
Woman at age 42 (No. 661)
Dermal density has been improved by 18.2% after 2 weeks and 36.4% after 4 weeks of use
Uneven surface of the epidermis has also been improved
03
In vivo
<Placebo>
<After 2 weeks>
<After 4 weeks>
in vivo Dermal Density Improvement
Woman at age 49 (No. 974)
Dermal density has been improved by 23.1% after 2 weeks and 46.2% after 4 weeks of use
Thickness of the dermis has also increased
03
In vivo
Placebo
After 2 weeks
After 4 weeks
in vivo Dermal Density Improvement
The test showed that dermal density has been gradually improved
Dermal density has been improved by 25.8% after 2 weeks and 37.4% after 4 weeks of use
For the subjects in 40s, they showed more improvement in dermal density by 35.6% after 4 weeks of use
04 Control Probiotics
Re-DerMAX
GFC Co., Ltd.
Control Probiotics
04
DGGE:
DGGE analysis using D-code system
Re-DerMAX 5% solution was applied to 5 volunteers
(normal group) and 7 volunteers (trouble group), 2 times
a day for 1 month to find out the change of skin flora
Control Probiotics
(Propionibacterium acnes)
Propionibacterium acnes
Acne vulgaris (or simply acne) is a
long-term skin disease that occurs
when hair follicles become clogged
with dead skin cells and oil from
the skin with bacteria called
Propionibacterium acnes.
Control Probiotics
(Propionibacterium acnes)
Skin Flora Test Method (Propionibacterium acnes)
Testing Agency: KDRI
Test: Effect of Re-DerMAX to check the change of skin flora
Test period: 2015. 06. 29 - 2015. 07. 31
Test subjects: 8 (1 woman in 20s, 2 women in 30s, and 5 men in 20s)
Testing method
Re-DerMAX was applied to the 8 of subjects, twice a day, in the morning and evening for three days
Assessment was performed using DGGE, and the content of bacteria was measured
Decrease in P.acnes after Treatment of Re-DerMAX
Subject No.
Re-DerMAX
P. acnes
1
-
+
2
-
+ -
3
+ -
4
+
-
5
+ -
6
7
+ - +
8
- +
Control Probiotics
(Staphylococcus aureus)
Staphylococcus aureus
Staphylococcus aureus is usually
found in the top layer of skin.
Although at the normal state, it
does not cause any harm, this
bacteria can provoke infection when
there is any wound or scar.
Control Probiotics
(Staphylococcus aureus)
Skin Flora Test Method (Staphylococcus aureus)
Testing Agency: GFC
Test: Effect of Re-DerMAX to check the change of skin flora
Test period: 2016. 03.28 – 2016.05.26
Test subjects: 2 (1 woman in 30s and 1 man in 30s)
Testing method
Re-DerMAX was applied to two subjects, twice a day, in the morning and evening for three days
Afterwards, quiescence period lasted for four days and retreatment was applied for three days
Assessment was performed using DGGE and the content of bacteria was measured
Subject No.
1
BE
S. aureus
AF
2
QS
RE
BE
AF
QS
RE
05 Conclusion
Re-DerMAX
GFC Co., Ltd.
05
Conclusion
Repairs UV-induced DNA damage
About 50% DNA of fibroblasts are repaired, compared to the control group
Provides a high elastase inhibitory activity at low concentration
Improves skin elasticity by 30% (after 4 weeks of use, in-vivo testing)
Improves dermal density by 34.7% (after 4 weeks of use, in-vivo testing)
Induces the change in skin flora using D-code DGGE systems within 1 month
Recommended Dosage: 5.0 ~ 10.0%
INCI EU cosing(PCPC): Bifida Ferment Lysate