CELL CULTURE MEDIA
AND REAGENTS
Classical Media
Buffered Salt Solutions
Reagents & Supplements
CELL CULTURE MEDIA AND REAGENTS | MEDIA
DULBECCO’S MODIFIED EAGLE’S MEDIUM (DMEM)
A modification of Basal Medium Eagle (BME), Dulbecco’s Modified Eagle's Medium (DMEM), that contains approximately
four times as much vitamins and amino acids and two to four times as much glucose as the original formulation. DMEM is
suitable for most types of cells, including human, monkey, hamster, rat, and mouse.
DMEM is available in a variety of formulations including high or low glucose, with or without sodium pyruvate, and with or
without L-glutamine.
Description
DMEM 4.5 g/L
Glucose,
w/ Sodium
Pyruvate and
L-Glutamine
DMEM 4.5 g/L
Glucose,
w/ Sodium
Pyruvate, w/out
L-Glutamine
DMEM 4.5 g/L
Glucose,
w/out Sodium
Pyruvate,
w/ L-Glutamine
DMEM 4.5 g/L
Glucose,
w/out Sodium
Pyruvate and
L-Glutamine
DMEM 1.0 g/L
Glucose,
w/ Sodium
Pyruvate and
L-Glutamine
DMEM 1.0 g/L
Glucose,
w/ Sodium
Pyruvate, w/out
L-Glutamine
Cat. No.
VWRL0101-0500
VWRL0148-0500
VWRL0100-0500
VWRL0102-0500
VWRL0111-0500
VWRL0149-0500
mg/L
mg/L
mg/L
mg/L
mg/L
mg/L
Glycine
30.00
30.00
30.00
30.00
30.00
30.00
L-Arginine · HCl
84.00
84.00
84.00
84.00
84.00
84.00
L-Cystine · 2HCl
63.00
63.00
63.00
63.00
63.00
63.00
L-Glutamine
584.00
0.00
584.00
0.00
584.00
0.00
L-Histidine HCl · H2O
42.00
42.00
42.00
42.00
42.00
42.00
L-Isoleucine
105.00
105.00
105.00
105.00
105.00
105.00
L-Leucine
105.00
105.00
105.00
105.00
105.00
105.00
L-Lysine · HCl
146.00
146.00
146.00
146.00
146.00
146.00
L-Methionine
30.00
30.00
30.00
30.00
30.00
30.00
L-Phenylalanine
66.00
66.00
66.00
66.00
66.00
66.00
L-Serine
42.00
42.00
42.00
42.00
42.00
42.00
L-Threonine
95.00
95.00
95.00
95.00
95.00
95.00
L-Tryptophan
16.00
16.00
16.00
16.00
16.00
16.00
L-Tyrosine · 2Na · 2H2O
104.00
104.00
104.00
104.00
104.00
104.00
L-Valine
94.00
94.00
94.00
94.00
94.00
94.00
CaCl2 (Anhydrous)
200.00
200.00
200.00
200.00
200.00
200.00
Fe(NO3) · 9H20
0.10
0.10
0.10
0.10
0.10
0.10
KCl
400.00
400.00
400.00
400.00
400.00
400.00
MgSO4 · 7H2O
199.99
199.99
199.99
199.99
199.99
199.99
NaHCO3
3700.00
3700.00
3700.00
3700.00
3700.00
3700.00
NaCl
6400.00
6400.00
6400.00
6400.00
6400.00
6400.00
NaH2PO4
125.00
125.00
125.00
125.00
125.00
125.00
Choline Chloride
4.00
4.00
4.00
4.00
4.00
4.00
D-Calcium Pantothenate
4.00
4.00
4.00
4.00
4.00
4.00
Folic Acid
4.00
4.00
4.00
4.00
4.00
4.00
i-Inositol
7.20
7.20
7.20
7.20
7.20
7.20
Niacinamide
4.00
4.00
4.00
4.00
4.00
4.00
Pyridoxine · HCl
4.00
4.00
4.00
4.00
4.00
4.00
Riboflavin
0.40
0.40
0.40
0.40
0.40
0.40
Thiamine · HCl
4.00
4.00
4.00
4.00
4.00
4.00
D-Glucose (Dextrose)
4500.00
4500.00
4500.00
4500.00
1000.00
1000.00
Phenol Red
15.00
15.00
15.00
15.00
15.00
15.00
Sodium Pyruvate
110.00
110.00
0.00
0.00
110.00
110.00
Components
AMINO
ACIDS
INORGANIC
SALTS
VITAMINS
OTHER
Description
DMEM, 4.5 g/L Glucose
w/ Sodium Pyruvate and L-Glutamine
w/ Sodium Pyruvate, w/out L-Glutamine
w/out Sodium Pyruvate, w/ L-Glutamine
w/out Sodium Pyruvate and L-Glutamine
DMEM, 1.0 g/L Glucose
w/ Sodium Pyruvate and L-Glutamine
w/ Sodium Pyruvate, w/out L-Glutamine
Size, mL
Cat. No.
Unit
500
500
500
500
VWRL0101-0500
VWRL0148-0500
VWRL0100-0500
VWRL0102-0500
Each
Each
Each
Each
500
500
VWRL0111-0500
VWRL0149-0500
Each
Each
2 VWR® CELL CULTURE MEDIA AND REAGENTS | PLACE YOUR ORDER TODAY AT VWR.COM, OR CALL 1.800.932.5000
RPMI-1640
RPMI-1640 was developed by Moore et. al. at Roswell Park Memorial Institute, hence the acronym RPMI. The
formulation is based on the RPMI-1630 series of media utilizing a bicarbonate buffering system and alterations
in the amounts of amino acids and vitamins. RPMI-1640, when properly supplemented, has demonstrated wide
applicability for supporting growth in many types of cultured cells.
Description
RPMI 1640 w/ L-Glutamine
RPMI 1640 w/out L-Glutamine
VWRL0105-0500
VWRL0106-0500
Components
mg/L
mg/L
Glycine
10.00
10.00
L-Arginine
200.00
200.00
L-Asparagine
50.00
50.00
L-Aspartic Acid
20.00
20.00
L-Cystine · 2HCl
65.00
65.00
L-Glutamic Acid
20.00
20.00
L-Glutamine
300.00
0.00
L-Histidine
15.00
15.00
L-Hydroxyproline
20.00
20.00
L-Isoleucine
50.00
50.00
L-Leucine
50.00
50.00
L-Lysine · HCl
40.00
40.00
L-Methionine
15.00
15.00
L-Phenylalanine
15.00
15.00
L-Proline
20.00
20.00
L-Serine
30.00
30.00
L-Threonine
20.00
20.00
L-Tryptophan
5.00
5.00
L-Tyrosine · 2Na · 2H2O
29.00
29.00
L-Valine
20.00
20.00
Ca(NO3)2 · 4H2O
100.00
100.00
MgSO4 · 7H2O
100.00
100.00
KCl
400.00
400.00
NaHCO3
2000.00
2000.00
NaCl
6000.00
6000.00
Na2HPO4 · 7H2O
1512.00
1512.00
Biotin
0.20
0.20
Choline Chloride
3.00
3.00
D-Calcium Pantothenate
0.25
0.25
Folic Acid
1.00
1.00
i-Inositol
35.00
35.00
Niacinamide
1.00
1.00
Para-Aminobenzoic Acid
1.00
1.00
Pyridoxine · HCl
1.00
1.00
Riboflavin
0.20
0.20
Thiamine · HCl
1.00
1.00
Vitamin B12
0.005
0.005
D-Glucose (Dextrose)
2000.00
2000.00
Glutathione (Reduced)
1.00
1.00
Phenol Red
5.00
5.00
Cat. No.
AMINO ACIDS
INORGANIC SALTS
VITAMINS
OTHER
Description
RPMI 1640 w/ L-Glutamine
RPMI 1640 w/out L-Glutamine
Size, mL
500
500
Cat. No.
VWRL0105-0500
VWRL0106-0500
Unit
Each
Each
PLACE YOUR ORDER TODAY AT VWR.COM, OR CALL 1.800.932.5000 | VWR® CELL CULTURE MEDIA AND REAGENTS 3
CELL CULTURE MEDIA AND REAGENTS | BUFFERED SALT SOLUTIONS
DULBECCO’S PHOSPHATE
BUFFERED SALINE (DPBS)
Dulbecco’s Phosphate Buffered Saline (DPBS) is a balanced salt solution used for the handling and culturing of
mammalian cells. Phosphate buffering maintains the pH in the physiological range. Calcium and magnesium
facilitate cell binding and clumping. DPBS without these ions can be used to wash and rinse suspended cells.
Description
DPBS (1X) w/ Ca and Mg
Cat. No.
Components
INORGANIC
SALTS
DPBS (1X) w/out Ca and Mg
VWRL0118-0500,
VWRL0118-1000
VWRL0119-0500,
VWRL0119-1000
g/L
g/L
CaCl2 (Anhdyrous)
0.1
0
MgCl2 · 6H2O
0.1
0
NaCl
8
8
KCl
0.2
0.2
Na2HPO4 · 7H2O
2.16
2.16
KH2PO4
0.2
0.2
Description
DPBS (1X) w/ Ca and Mg
DPBS (1X) w/ Ca and Mg
DPBS (1X) w/out Ca and Mg
DPBS (1X) w/out Ca and Mg
Size, mL
500
1000
500
1000
Cat. No.
VWRL0118-0500
VWRL0118-1000
VWRL0119-0500
VWRL0119-1000
Unit
Each
Each
Each
Each
HANK’S BALANCED SALT
SOLUTION (HBSS)
Hank’s Balanced Salt Solution (HBSS) is a buffer used to maintain a physiological pH
for cells maintained in non-CO2 atmospheric conditions. HBSS can be used for washing cells or as a solution for
transporting cells and tissue culture.
HBSS (1X) w/out Ca, Mg, and Phenol Red
VWRL0121-0500
Components
INORGANIC SALTS
OTHER
g/L
% of Solution
NaCl
8
0.8%
KCl
0.4
0.04%
Na2HPO4 (Anhydrous)
0.048
0.005%
KH2PO4 (Monobasic)
0.06
0.01%
NaHCO3
0.35
0.04%
D-Glucose (Dextrose)
1
0.1%
Description
HBSS (1X) w/out Ca, Mg, and Phenol Red
Size, mL
500
Cat. No.
VWRL0121-0500
Unit
Each
4 VWR® CELL CULTURE MEDIA AND REAGENTS | PLACE YOUR ORDER TODAY AT VWR.COM, OR CALL 1.800.932.5000
WATER, STERILE, LOW ENDOTOXIN,
TISSUE CULTURE GRADE
Water, sterile, low endotoxin, cell culture grade is ideal for use in the preparation
of cell culture media and reagents.
Test
Other
pH
Check and Record
Sterility
Negative
Total Organic Carbon (Before Packaging)
NMT 500 ppb
Conductivity (Before Packaging)
NMT 1.3 µs/cm
Endotoxin
NMT 0.25 EU/mL
Storage Conditions
15°C to 30°C
For Further Manufacturing or Research Use. Not for Therapeutic Applications.
Size
500 mL
1L
Cat. No.
VWRL0200-0500
VWRL0200-1000
Unit
Each
Each
AMINO ACIDS
Amino acids supply nutrients to cells in culture supporting growth.
Description
Charged Amino Acids
L-Arginine
L-Arginine
L-Aspartic Acid
L-Aspartic Acid
L-Glutamic Acid
L-Glutamic Acid
L-Histidine
L-Histidine
L-Histidine
L-Histidine
L-Histidine
L-Histidine
L-Lysine
L-Lysine
Hydrophobic Amino Acids
L-Alanine
L-Alanine
L-Alanine
L-Glycine, Biotechnology Grade
L-Glycine, Proteomics Grade
L-Glycine, Biotechnology Grade
L-Glycine, Proteomics Grade
L-Glycine
Size
Cat. No.
Unit
100 g
500 g
500 g
1 kg
1 kg
2.5 kg
25 g
100 g
100 g
500 g
500 g
1 kg
100 g
500 g
97062-466
97062-468
97061-188
97061-186
97061-634
97061-636
97063-020
97063-018
97062-598
97062-602
97064-022
97062-600
97061-666
97061-668
Each
Each
Each
Each
Each
Each
Each
Each
Each
Each
Each
Each
Each
Each
100 g
250 g
500 g
1 kg
1 kg
5 kg
5 kg
10 kg
97061-024
97061-026
97061-028
97061-128
97063-736
97061-132
97063-738
97061-124
Each
Each
Each
Each
Each
Each
Each
Each
Description
Hydrophobic Amino Acids
L-Glycine
L-Glycine
L-Isoleucine
L-Isoleucine
L-Leucine
L-Leucine
L-Leucine
L-Methionine
L-Methionine
L-Methionine
L-Phenyalanine
L-Phenyalanine
L-Phenyalanine
L-Proline
L-Proline
L-Proline
L-Typtophan
L-Typtophan
L-Typtophan
L-Valine
L-Valine
L-Valine
Size
12 kg
50 kg
25 g
100 g
25 g
100 g
250 g
25 g
100 g
250 g
25 g
100 g
500 g
25 g
100 g
500 g
25 g
100 g
500 g
25 g
100 g
500 g
Cat. No.
Unit
97061-126
97061-130
97064-016
97064-014
97064-034
97064-030
97063-032
97064-010
97063-008
97063-012
97062-558
97062-556
97062-560
97064-038
97064-036
97064-040
97064-004
97064-002
97064-006
97062-564
97062-562
97062-566
Each
Each
Each
Each
Each
Each
Each
Each
Each
Each
Each
Each
Each
Each
Each
Each
Each
Each
Each
Each
Each
Each
PLACE YOUR ORDER TODAY AT VWR.COM, OR CALL 1.800.932.5000 | VWR® CELL CULTURE MEDIA AND REAGENTS 5
CELL CULTURE MEDIA AND REAGENTS | REAGENTS AND SUPPLEMENTS
ANTIBIOTICS
L-GLUTAMINE
Antibiotics are used to prevent contamination in cell
culture. The use of antibiotics in cell culture is dependent
on the needs of the researcher.
L-glutamine is an essential amino acid. It is used as
an energy source for cells in cell culture. L-glutamine
is stable as a dry powder and as a frozen solution but degrades rapidly in liquid
media or stock solutions.
Description
Ampicillin, Gamma Irradiated, Tissue Culture
Grade
Ampicillin, Sodium Salt, USP Grade
Ampicillin, Sodium Salt, USP Grade
Ampicillin, Trihydrate, USP Grade
Ampicillin, Trihydrate, USP Grade
Ampicillin, Trihydrate, USP Grade
Carbenicillin, Disodium Salt, Ultra Pure Grade
Carbenicillin, Disodium Salt, Ultra Pure Grade
Penicillin G, Sodium Salt, USP Grade
Penicillin G, Sodium Salt, USP Grade
Penicillin G, Sodium Salt, Irradiated, Tissue
Culture Grade
Penicillin Streptomycin, Irradiated, Tissue
Culture Grade
Size
20 mL
Cat. No.
97062-796
Unit
Each
20 g
100 g
50 g
100 g
500 g
250 mg
1g
100 MU
1 BU
2 mL
97061-442
97061-440
97062-300
97062-296
97062-298
97063-146
97063-144
97061-280
97061-282
97062-798
Each
Each
Each
Each
Each
Each
Each
Each
Each
Each
20 mL
97062-806
Each
L-Glutamine, 200mM (100X)
VWRL0131-0100
Components
g/L
L-Glutamine
29.2
NaCl
8.5
Description
L-Glutamine, 200mM (100X)
Size
500 g
1 kg
2.5 kg
12 kg
50 kg
Cat. No.
97061-008
97061-004
97061-006
97061-002
97061-010
Unit
Each
Each
Each
Each
Each
DIMETHYL SULFOXIDE,
ULTRAPURE (DMSO)
•
•
•
Used in cell culture applications
>99.9% pure
Sterile tested
Description
Dimethyl Sulfoxide, Ultrapure
Size
5 x 10 mL
Cat. No.
97063-136
Unit
Each
Unit
Each
Cat. No.
97062-832
Unit
Each
Cryoprotectant used in cell freezing
Description
Glycerol, Sterile, Biotechnology Grade
A biological buffer used for cell culture, in vitro, enzyme assays and some electrophoretic applications at physiological pH.
Cat. No.
VWRL0131-0100
GLYCEROL, STERILE,
BIOTECHNOLOGY GRADE
•
CITRIC ACID, TRISODIUM
SALT, DIHYDRATE
Size, mL
100
Size
100 mL
SERAFREE™
CRYOPRESERVATION
MEDIA
Sterile and endotoxin tested
Reduces potential for transmission of infectious agents
• Eliminates regulatory compliance requirements associated with use of
animal products
• Cost effective
SeraFree™ Cryopreservation Media are ready-to-use freezing media for cryopreservation of adherent or suspension cultured cells. The animal-free RPMI- or DMEMbased media composition eliminates batch-to-batch variability and is optimized for
cell viability and cell growth after thawing.
•
•
Description
Serafree™ Cryopreservation Media for DMEM
Serafree™ Cryopreservation Media for RPMI
Serafree™ Cryopreservation Media for RPMI
Size
50 mL
6 x 5 mL
50 mL
Cat. No.
97063-278
97064-278
97064-276
Unit
Each
Each
Each
SODIUM CHLORIDE
Sodium chloride is a common source of salts for media
propagation of many organisms.
Size
500 g
1 kg
2.5 kg
5 kg
10 kg
12 kg
50 kg
100 kg
6 VWR® CELL CULTURE MEDIA AND REAGENTS | PLACE YOUR ORDER TODAY AT VWR.COM, OR CALL 1.800.932.5000
Cat. No.
97061-274
97061-270
97061-272
97061-278
97061-266
97061-268
97061-276
97061-264
Unit
Each
Each
Each
Each
Each
Each
Each
Each
SUGARS
TRYPSIN (0.25%) EDTA
High purity sugars are used for media supplements and
are a primary source of energy for cells in culture.
Trypsin (0.25%) EDTA (1X) is used to release adherent
cells from tissue culture plasticware for harvesting and
passaging. Contains 0.001% phenol red, 0.04% Trypsin, 0.25% EDTA.
Description
D-Fructose
D-Fructose
D-Fructose
D-Fructose
D-(+)-Galactose
D-(+)-Galactose
D-(+)-Galactose
D-Glucose, Anhydrous
D-Glucose, Anhydrous
D-Glucose, Anhydrous
D-Glucose, Anhydrous
D-Glucose, Anhydrous
D-Glucose, Anhydrous
Glucose, 20% Solution, Sterile
Maltose Monohydrate
Maltose Monohydrate
Maltose Monohydrate
Size
1 kg
2.5 kg
5 kg
12 kg
100 g
250 g
500 g
500 g
1 kg
2.5 kg
5 kg
12 kg
50 kg
100 mL
100 g
500 g
1 kg
Cat. No.
97061-236
97061-238
97061-242
97061-234
97062-076
97062-078
97062-080
97061-168
97061-164
97061-166
97061-172
97061-162
97061-170
97062-880
97062-604
97062-608
97062-606
Unit
Each
Each
Each
Each
Each
Each
Each
Each
Each
Each
Each
Each
Each
Each
Each
Each
Each
Trypsin (0.25%) EDTA (1X)
VWRL0154-0100
Components
INORGANIC
SALTS
OTHER
g/L
% of Solution
NaCl
8
0.8%
KCl
0.4
0.04%
Na2HPO4 · 7H2O
0.091
0.009%
KH2PO4 (Monobasic)
0.06
0.01%
NaHCO3
0.35
0.04%
Phenol Red
0.01
0.0010%
EDTA · 4Na · 2H2O
0.38
0.04%
Trypsin (1:250)
2.5
0.25%
D-Glucose (Dextrose)
1
0.1%
Description
Trypsin (0.25%) EDTA (1X)
Size, mL
100
Cat. No.
VWRL0154-0100
Unit
Each
ALL YOU NEED
Solutions for Science
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of science throughout the world. We do this by
developing deep connections with our customers.
We have a commitment to provide the best-in-class
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TISSUE CULTURE PLASTICWARE
VWR Collection Cell Culture Volume and Area Guide
Description
VWR® Standard
Line Multiwell Cell
Culture Plates
6 Well
Size
Working
Volume, mL
Cell Growth
Area, cm2
Max Cell
Yield**
Cat. No.
17.0 mL
1.90 - 2.90
9.6
9.6 x 105
10062-892
3.85 x 105
10062-894
12 Well
6.80 mL
0.76 - 1.14
3.85
24 Well
3.50 mL
0.38 - 0.57
1.93
1.93 x 105
10062-896
48 Well
1.55 mL
0.19 - 0.29
0.84
8.4 x 104
10062-898
96 Well,
Flat Bottom
0.39 mL
0.075 - 0.20
0.33
3.3 x 104
10062-900
96 Well,
U Bottom
0.32 mL
0.075 - 0.20
0.32
3.2 x 104
10062-902
35 Dia. x 12 H, mm
1.7 - 4.25
8.5
8.5 x 105
10062-888
90 Dia. x 17 H, mm
11 - 27.5
55
5.5 x 106
10062-878
150 Dia. x 22 H, mm
28.6 - 71.5
143
1.43 x 107
10062-882
VWR® Standard Line
Cell Culture Dishes, Gripping Ring
for Improved Handling
60 Dia. x 18 H, mm
4.24 - 10.6
21.2
2.12 x 106
10062-890
70 Dia. x 15 H, mm
7.26 - 18.15
36.3
3.63 x 106
10062-876
100 Dia. x 22 H, mm
12.16 - 30.4
60.8
6.08 x 106
10062-880
VWR® Standard Line Cell Culture
T-Flasks, Non-vented Caps
12.5 cm2 (25 mL)
2.5 - 6.25
12.5
1.25 x 106
10062-870
25 cm (50 mL)
5 - 12.5
25
2.5 x 106
10062-874
75 cm (250 mL)
15 - 37.5
75
7.5 x 10
10062-862
182.5 cm2 (600 mL)
36.5 - 91.25
182.5
1.83 x 107
10062-866
300 cm2 (850 mL)
60 - 150
300
3 x 107
10062-886
2/Bag, 40/Case
1,000 mL
100 - 150
490
4.9 x 107
10769-794
2/Bag, 20/Case
2,000 mL
175 - 250
850
8.5 x 10
7
10769-796
5/Bag, 40/Case
2,000 mL
175 - 250
850
8.5 x 107
10769-798
VWR® Standard Line
Cell Culture Dishes
2
2
VWR® Roller
Bottles, Surface
Treated
•  Crystal clear virgin PS - sterile and
certified non-pyrogenic
•  Plasma treated for consistent cell
attachment and growth
•  Well volume uniformity ensures uniform
growth surface area exposure
•  Single position lid reduces misplacement
6
and the risk of contamination
•  Crystal clear virgin PS - sterile and
certified non-pyrogenic
•  Plasma treated for consistent cell
attachment and growth
•  Flat bottom and uniform wall thickness
ensure dishes are distortion-free
•  Optically clear, making them suitable for
microscopy
•  Angled neck design offers good pipette
and cell scraper access
•  Plasma treated for consistent cell
attachment and growth
•  Crystal clear virgin PS - sterile and
certified non-pyrogenic
•  Also available with vented caps
•  Sterilized, non-pyrogenic
•  Bottle surface is smooth and free from
striation to maximize usable growth area
•  Two different cap styles can be used in
2/Bag, 40/Case
2,000 mL
175 - 250
850
8.5 x 107
5/Bag, 40/Case
2,000 mL
380 - 570
(Ribbed Design)
1900
1.9 x 10
125 mL
21.3 - 50
–
–
89095-260
250 mL 62.5 - 100
–
–
89095-268
500 mL
125 - 200
–
–
89095-276
1L
250 - 400
–
–
89095-284
2L
500 - 800 –
–
10126-438
3 L 750 - 1200
–
–
10126-410
125 mL
21.3 - 50
–
–
89095-264
250 mL 62.5 - 100
–
–
89095-272
500 mL
125 - 200
–
–
89095-280
1L
250 - 400
–
–
89095-288
2L
500 - 800 –
–
10126-408
3 L 750 - 1200
–
–
10126-412
VWR® Erlenmeyer Flasks, Flat
Base, Non-vented Caps
VWR® Erlenmeyer Flasks, Baffled
Base*, Non-vented Caps
8
10769-800
10769-802
both open and closed systems
•  Cat. No. 10769-802 features a rib design
•  Virgin, medical-grade resin retains its
clarity after being exposed to gamma
radiation
•  Non-pyrogenic, inert, and nonleaching
•  Excellent for non-adhering/suspension
cultures
•  Also available with vented caps
•  2L and 3L flasks with convenient vented
duo-cap makes switching caps easy;
simply remove the dustguard cover to
allow sterile exchange, then replace the
cover
* Baffled base flasks may allow higher working volumes or low shaking speeds
** Based on cell diameter 10µm
0616 5M Lit. No. 93612