University of Tabuk
Faculty of Applied Medical Science
Department of Medical Laboratory Technology
2012/2013
Fourth Year Clinical Rotations Booklet
Table of Contents
OVERALL OUTCOMES GOALS ..............................................................................................................................................3
HEMATOLOGY CLINICAL ROTATION....................................................................................................................................5
MICROBIOLOGY CLINICAL ROTATION .................................................................................................................................6
IMMUNOLOGY CLINICAL ROTATION ....................................................................................................................................8
BLOOD BANK CLINICAL ROTATION ..........................................................................................................................................9
CLINICAL CHEMISTRY (BIOCHEMISTRY) CLINICAL ROTATION ........................................................................................10
Overall Outcomes Goals
At the conclusion of the laboratory rotation, the student shall be able to:

List the minimum requirement for an acceptable laboratory request form.

Identify incorrect or inappropriate specimens.

Knowing the appropriate means of informing the nursing station or physician of the inappropriate specimen.

Identify the preferred specimen to be used for the selected test in each department.

Identify the storage requirements for specimens for selected procedures in each department.


Correctly process laboratory specimens received in the department, including centrifugation, serum or plasma
separation, and storage containers.
Explain the principle of the selected laboratory procedures performed in the laboratory.

Explain patient collection procedures for random urine, clean catch urine, and 24 hour urine.

Prepare a plan of appropriate safety procedures for each department in the lab.

Demonstrate proper techniques including manual and/or automated techniques

Demonstrate the proper procedure for disposing of contaminated materials; including plates, pipettes, pipette
tips; specimen tubes or containers glass slides, needles and syringes.

Differentiate between control, standard, and calibrator.

Understand the quality control program for each laboratory department.

Properly record daily freezer, Water-bath, refrigerator temperature readings.

State the normal ranges of the selected tests in each department, including age and sex variations.

Interpret patients test results as normal or abnormal, identify possible disease states.

List interfering substances and source of errors for the selected test within each department.

Differentiate between false positive and false negative results.

Identify causes for false negative and false positive results for the selected tests in each department.

Suggest possible solutions to technical problems that the student encounters during the clinical rotation.

Report problems to the clinical instructor when they are detected.
Please read above checklist and tick on each items and sign
Student signature: ……………………….
Hematology Clinical Rotation
No Recommended Test Name by the university
1
2
3
4
5
Comments
Perform CBC using automated Hematology
analyzer.
Identify CBC parameters and Interprets
Histograms of the automated Hematology
analyzer
Manually prepare and stain blood films
7
Manually perform leukocyte differential count
on Normal & abnormal samples
10
Not Done
Daily maintenance, sample loading, reagents
loading and processing of controls in the
Hematology analyzer.
Examine stained blood films for RBCs
morphology and platelets estimates
9
Done
Specimens &anticoagulants in Hematology lab.
6
8
Name an alternative test if the
recommended test is not
available
Perform Prothrombin Time(PT) & Activated
Partial thromboplstin Time( APTT )tests
Perform a Erythrocyte Sedimentation Rate (ESR)
within 5% of the measured value.
Perform the sickle cell screening test
Perform Reticulocyte count with results
11 acceptable to the instructor.
Student signature: …………………………………………………………
Supervisor signature: ………………………………………………………
Microbiology Clinical Rotation
No Recommended Test Name by the university
1
2
3
4
5
Prepare a bacterial smear and stain the
processed clinical specimens:
• Gram stain
• Acid fast stain
Perform microscopic identification
(morphology, stain reaction)
Demonstrate the diagnostic tests and
interpret the results
•Catalase test
•Coagulase test
•Oxidase test
Prepare and identify the basic culture
media
•Blood Agar Plate (BAP)
•MacConkey Agar (MAC)
•Chocolate Agar Plate (CAP)
•Mueller Hinton Agar ( MHA)
•Blood Culture
•CLED agar
•Salmonella Shigella Agar (SSA)
Cultural & Microscopic Morphology and
Specific Identification tests or flow chart
tests for:
•Staphylococci
•Streptococci
•Non-spore-forming Gram Positive Rods
•Aerobic Gram Positive Bacilli
•Neisseria
•Fastidious gram negative rods
•Enterobacteriaceae (Escherichia,
Klebsiella, Salmonella, Proteus, etc.)
Name an alternative test if
the recommended test is
not available
Done
Not Done
Comments
6
7
8
9
10
11
12
Make bacterial suspension to be used for
identification or Antibiotic Sensitivity Test
(AST)
Perform Disk diffusion antibiotic Sensitivity
for isolated bacteria and measurement of
zone of inhibition
Describe the different hemolysis pattern in
Blood Agar Plate
Perform disc sensitivity and interpret test
•Optochin
•Novobiocin
Interpret Lactose fermentation test in
MacConkey agar
Use specific indicator for identification of
certain bacteria
•Pigment
•Colonies
•Swarming
•Odor
•Etc
Perform Other automated methods, like
Bactec, Vitek and Autoclave.
Student signature: …………………………………………………………
Supervisor signature: …………………………………………………
Immunology Clinical Rotation
No
1
Recommended Test Name by the university
Name an alternative test if
the recommended test is not
available
Done
Not Done
Comments
Latex agglutination:
A. Widal Test.
B.Brucella (Rose Bengal) Test.
C.Rhematoid Factor (RF).
D. C-Reactive Protein (CRP).
E. Anti-Streptolysin O (ASO).
2
3
4
Syphilis tests :
Rapid Plasma Reagin ( RPR)&Venereal Disease
Research Laboratories(VDRL)
Enzyme-Linked Immunosorbent Assay (ELISA)
applications
Titration, Dilutions, & Serial dilution
Student signature: …………………………………………………………
Supervisor signature: ………………………………………………
Blood Bank Clinical Rotation
No Recommended Test Name by the university
Name an alternative
test if the
recommended test is
not available
Done
Not Done
Comments
1 Donors selection
Questionnaire, physiological parameters, initial
blood grouping, pre-donation deferral.
2 Phlebotomy
Labeling, blood bags, tubes, blood collection.
3 Blood components
Labeling, Blood bags, centrifugation &
separation, storage, expiration, transport,
blood components.
4 Donors Screening Tests
Testing for infectious pathogens of transfusion
transmitted diseases
5 ABO/Rh
Slide method,Tube method(Forward , Reverse),
card method, weak-D test
6 Coombs test (AHGT)
Direct / Indirect.
7 Cross match
8 Antibody Screening
9 Products preparation
PRBCs, Fresh Frozen Plasma (FFP), Platelets,
Cryoprecipitate.
Student signature: …………………………………………………………
Supervisor signature: …………………………………………
Clinical Chemistry (Biochemistry) Clinical Rotation
No Recommended Test Name by the university
1
Identify and assay patient specimens and the
anticoagulants or preservatives used according to
established procedures.
2
Identify the reasons for rejecting patient's
samples in clinical chemistry lab.
3
Centrifugation, separation and processing of
whole blood samples to obtain serum and
plasma samples.
Know how to deal with the clinical Chemistry
analyzer in: data entry, specimen loading,
reagents loading and control preparation.
4
5
Perform serum electrolytes with results
acceptable to the instructor.
6
Know how to Perform Glucose ( Random, Fasting)
7
List and perform Liver Function Tests (LFT)
8
List and perform Kidney Function Tests (KFT)
9
10
List and perform Lipid profile tests
Know how to perform certain Enzymes ( Liver
enzymes, cardiac enzymes, Pancreatic
enzymes)
Name an alternative
test if the
recommended test is
not available
Done
Not Done
Comments
Student signature: ……………………………………………………… Supervisor signature: ………………………………………………………
Clinical Rotation
HEMATOLOGY CLINICAL ROTATION
MICROBIOLOGY CLINICAL ROTATION
IMMUNOLOGY CLINICAL ROTATION
BLOOD BANK CLINICAL ROTATION
CLINICAL CHEMISTRY (BIOCHEMISTRY) CLINICAL ROTATION
Hospital
Department
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