solution

DIN EN ISO 9001:2008 CERTIFIED
NTP Specifications
Nomenclature
ATP; 100mM Sodium Salt Solution
Adenosine 5’-triphosphate
CTP, 100mM Sodium Salt Solution
Cytidine 5’-triphosphate
GTP, 100mM Sodium Salt Solution
Guanosine 5’-triphosphate
UTP, 100mM Sodium Salt Solution
Uridine 5’-triphosphate
CAS Number
987-65-5
36051-68-0
36051-31-7
19817-92-6
ATP_100ML
CTP_100ML
GTP_100ML
UTP_100ML
ATP_1000ML
CTP_1000ML
GTP_1000ML
UTP_1000ML
C10H14N5Na2O13P3 x 3 H2O
C9H14N3Na2O14P3 x 3 H2O
C10H14N5Na2O14P3 x 3 H2O
C9H12N2Na3O15P3 x 3 H2O
Formula Weight (g x mol )
504.16
480.16
520.15
481.11
Storage at
-20 °C
-20 °C
-20 °C
-20 °C
Stability
24 months
24 months
24 months
24 months
clear colorless solution
clear colorless solution
clear colorless solution
clear colorless solution
100 mM ± 2% (A259nm,ɛ=15,1 l x
100 mM ± 2% (A271nm,ɛ=8,9 l x
100 mM ± 2% (A252nm,ɛ=14,2 l x
100 mM ± 2% (A262nm,ɛ=9,8 l x
Catalog Number
Formula (Anion)
-1
Appearance
Concentration
(1)
(22°C, pH 7)
-1
-1
-1
-1
-1
-1
-1
-1
mmol x cm )
mmol x cm )
mmol x cm )
mmol x cm )
A250 nm / A260 nm (22 °C, pH 7.0)
0.78 ± 0.02
0.82 ± 0.02
1.15 ± 0.03
0.74 ± 0.02
A280 nm / A260 nm (22 °C, pH 7.0)
0.15 ± 0.01
0.97 ± 0.02
0.67 ± 0.02
0.38 ± 0.02
A290 nm / A260 nm (22 °C, pH 7.0)
NA
0.30 ± 0.02
0.28 ± 0.02
0.04 ± 0.01
pH (4°C)
8,0±0.2
8,0±0.2
8,0±0.2
8,0±0.2
NTP (HPLC area)
≥ 99.0%
≥ 99.0%
≥ 99.0%
≥ 99.0%
NDP (HPLC area)
≤ 1.0%
≤ 1.0%
≤ 1.0%
≤ 1.0%
in vitro Transcription (T7 RNA
visible RNA fragment after 5 min
visible RNA fragment after 5 min
visible RNA fragment after 5 min
visible RNA fragment after 5 min
polymerase, 1423 bp template)
incubation
incubation
incubation
incubation
DNases, RNases, Nicking Activity
not detectable
not detectable
not detectable
not detectable
Proteases
not detectable
not detectable
not detectable
not detectable
(1) Extinction coefficient: Cavaluzzi & Borer (2004) Nucleic Acids Res. 32(1):e13