DIN EN ISO 9001:2008 CERTIFIED NTP Specifications Nomenclature ATP; 100mM Sodium Salt Solution Adenosine 5’-triphosphate CTP, 100mM Sodium Salt Solution Cytidine 5’-triphosphate GTP, 100mM Sodium Salt Solution Guanosine 5’-triphosphate UTP, 100mM Sodium Salt Solution Uridine 5’-triphosphate CAS Number 987-65-5 36051-68-0 36051-31-7 19817-92-6 ATP_100ML CTP_100ML GTP_100ML UTP_100ML ATP_1000ML CTP_1000ML GTP_1000ML UTP_1000ML C10H14N5Na2O13P3 x 3 H2O C9H14N3Na2O14P3 x 3 H2O C10H14N5Na2O14P3 x 3 H2O C9H12N2Na3O15P3 x 3 H2O Formula Weight (g x mol ) 504.16 480.16 520.15 481.11 Storage at -20 °C -20 °C -20 °C -20 °C Stability 24 months 24 months 24 months 24 months clear colorless solution clear colorless solution clear colorless solution clear colorless solution 100 mM ± 2% (A259nm,ɛ=15,1 l x 100 mM ± 2% (A271nm,ɛ=8,9 l x 100 mM ± 2% (A252nm,ɛ=14,2 l x 100 mM ± 2% (A262nm,ɛ=9,8 l x Catalog Number Formula (Anion) -1 Appearance Concentration (1) (22°C, pH 7) -1 -1 -1 -1 -1 -1 -1 -1 mmol x cm ) mmol x cm ) mmol x cm ) mmol x cm ) A250 nm / A260 nm (22 °C, pH 7.0) 0.78 ± 0.02 0.82 ± 0.02 1.15 ± 0.03 0.74 ± 0.02 A280 nm / A260 nm (22 °C, pH 7.0) 0.15 ± 0.01 0.97 ± 0.02 0.67 ± 0.02 0.38 ± 0.02 A290 nm / A260 nm (22 °C, pH 7.0) NA 0.30 ± 0.02 0.28 ± 0.02 0.04 ± 0.01 pH (4°C) 8,0±0.2 8,0±0.2 8,0±0.2 8,0±0.2 NTP (HPLC area) ≥ 99.0% ≥ 99.0% ≥ 99.0% ≥ 99.0% NDP (HPLC area) ≤ 1.0% ≤ 1.0% ≤ 1.0% ≤ 1.0% in vitro Transcription (T7 RNA visible RNA fragment after 5 min visible RNA fragment after 5 min visible RNA fragment after 5 min visible RNA fragment after 5 min polymerase, 1423 bp template) incubation incubation incubation incubation DNases, RNases, Nicking Activity not detectable not detectable not detectable not detectable Proteases not detectable not detectable not detectable not detectable (1) Extinction coefficient: Cavaluzzi & Borer (2004) Nucleic Acids Res. 32(1):e13
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