Section 1: Chemical Identification
Chemical name: 3,3’,5,5’-Tetramethylbenzidine, free base
Identity (As used on label and list): 3,3’,5,5’-Tetramethylbenzidine, free base
Synonyms: TMB
Catalog number: TMBF2.5, TMBF5, TMBF10, TMBF50
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Section 2: Composition/Information on Ingredients
CAS No.
[54827-17-7]
Chemical name
3,3’,5,5’-Tetramethylbenzidine
Percent
100
EINECS
259-364-6
Classification
Non-hazardous product
Section 3: Hazardous Ingredients/Identity Information
Emergency Overview
Caution! To the best of our knowledge the toxicological properties of this material have not been
thoroughly investigated. May be harmful if swallowed, inhaled or absorbed through skin.
Target Organs
none
Potential Health Effects
Eye
Causes eye irritation
Skin
Causes skin irritation
Ingestion
Causes digestive tract irritation
Inhalation
Causes respiratory tract irritation
Chronic
May cause possible mutagen effects
Section 4: First Aid Measures
Eye
Skin
Ingestion
Inhalation
Notes to Physician
In case of contact, immediately flush eyes with copious amounts of water for at
least 15 minutes. Assure adequate flushing by separating the eyelids with fingers.
Call a physician.
In case of contact, wash skin with soap and water for at least 15 minutes while
removing contaminated clothing and shoes. Call a physician. Wash
contaminated clothing before reuse.
If swallowed, do not induce vomiting. Allow the victim to wash out his mouth with
water and to drink 2-4 cups of water provided person is conscious. Call a
physician.
If inhaled, remove to fresh air, immediately. If not breathing give artificial
respiration. If breathing is difficult, give oxygen. Call a physician.
Treat symptomatically and supportively.
Section 5: Fire Fighting Measures
General Information
Extinguishing Media
Autoignition Temperature
Flash Point
NFPA Rating
As in any fire, wear self-contained breathing apparatus and protective
clothing to prevent contact with skin and eyes. During a fire, irritating and
highly toxic gases may be generated by thermal decomposition or
combustion.
In case of fire use water spray, carbon dioxide, dry chemical powder or
appropriate foam.
Not available
Not available
Not published. Explosion Limits, Lower: Not available. Upper: Not available.
Section 6: Accidental Release Measures
General Information
Spills/Leaks
Use proper personal protective equipment as indicated in Section 8.
Sweep up, place in a bag and hold for waste disposal; avoid raising dust;
ventilate area and wash spill site after material pickup is complete.
Section 7: Handling and Storage
Handling
Storage
Avoid ingestion and inhalation. Do not breathe dust. Avoid contact with skin, eyes and
clothing. Avoid prolonged or repeated exposure. Wash thoroughly after handling.
Remove contaminated clothing and wash before reuse.
Keep container tightly closed when not in use. Refrigerate (2-8°C). Store dry. Protect from
light. Air sensitive. Protect from humidity (material is hygroscopic). Handle and store under
nitrogen.
Section 8: Control Measures
Engineering Controls Use adequate general or local exhaust ventilation to keep airborne
concentrations below the permissible exposure limits.
Exposure Limit OEL-Australia; (Prohibited) Skin; Carcinogen OEL-Finland;
Personal Protective Equipment
Eyes
Wear chemical safety goggles or face shield if splashing is possible.
Skin
Wear compatible chemical-resistant gloves and clothing to prevent skin
exposure.
Hand
Wear compatible chemical-resistant gloves to prevent skin exposure.
Clothing
Wear appropriate protective clothing to prevent exposure.
Respirator
Wear a NIOSH/MSHA or European Standard EN 149 approved full-face airline
respirator in the positive pressure mode with emergency escape provisions.
Section 9: Physical And Chemical Properties
Appearance
Odor
pH
Boiling Point
Melting Point
Flash Point
Autoignition Temperature
Vapor Pressure
Vapor Density
Specific Gravity/Density
Coefficient octanol/water
Solubility in water
Solubility other
Other physical data
Molecular Formula
Molecular Weight
off-white to yellowish powder
Characteristic odor
Not available.
Not applicable
166 - 170° C
Not available
Not available
Not applicable
Not applicable
Not applicable
Not available
Hardly soluble in water
Soluble in acetone
Not available
C16H20N2
240.35 g/mol
Section 10: Stability And Reactivity Data
Stability
Conditions to Avoid
Incompatibilities
Hazardous Decomposition Products
Hazardous Polymerization
Stable
Incompatible materials, light, air, moisture
Strong oxidizing agents
Toxic fumes of: carbon monoxide, carbon dioxide, nitrogen oxides
Will not occur
Section 11: Toxicological Information
RTECS
CAS No.
LD50/LC50
Carcinogenity
DV 2300000
[54827-17-7]
IPR-MUS LD50: 135 mg/kg ORL-BIRD LD50: > 316 mg/kg
ACGIH: A1-confirmed human carcinogen; NIOSH: occupational carcinogen; NTP:
Known carcinogen; OSHA: Select carcinogen; IARC: Group1 carcinogen (all listed
as Benzidine)
Section 11: Toxicological Information (continued)
Epidemiology
Teratogen
Reproductive Effects
Neurotoxicity
Mutagen
Other Studies
Not available.
Not available.
Not available.
Not available.
May cause possible mutagen effects.
Listed as Benzidine at all carcinogenity regulations. Only selected Registry of toxic
effects of chemical substances (RTECS) data is presented here. See actual entry
in RTECS for complete information.
Section 12: Ecological Information
Ecotoxicity
Environmental Fate
Physical/Chemical
Other
Not available
Not available
Not available
Not available
Section 13: Disposal Considerations
Disposal should be handled by an approved waste disposal facility
Section 14: Transport Information
IATA
UN Number
Proper Shipping Name
Class
Packing Group
RID/ADR
UN Number
Proper Shipping Name
Class
Packing Group
Not available
Not regulated as a hazardous material. (IATA, RID/ADR)
Not available
Not available
Not available
Not regulated as a hazardous material. (IATA, RID/ADR)
Not available
Not available
Section 15: Regulatory Information
European / International Regulations
Hazard Symbols
Not available
R-Phrase No.
Not available
Risk Phrases
Not available
S-Phrase No.
S22/S24/S25
Safety Phrases
Do not breathe dust. Avoid contact with skin and eyes.
International Regulations
Carcinogen OEL-France: TWA 0.001 ppm (0.008 mg/m3); Carcinogen OEL-Germany; Skin; Carcinogen
OEL-The Netherlands; Skin OEL-Sweden; Carcinogen OEL-Switzerland; Carcinogen OEL in Bulgaria,
Colombia, Jordan, Korea check ACGIH TLV OEL in New Zealand, Singapore, Vietnam check ACGI TLV
Various
Exposure Limit
OEL-Australia; (Prohibited) Skin; Carcinogen OEL-Finland;
Section 16: Other Information
While Gold Biotechnology, Inc.™ believes the information contained herein to be true and accurate, it
has relied on information provided by others. Gold Biotechnology, Inc.™ makes no warranties, express
or implied, as to the accuracy or adequacy of the information contained herein or with respect to the
results to be obtained from the use of the product. Gold Biotechnology, Inc.™ disclaims all liability with
respect to the use of this product, including without limitation, liability for injury to the user or third-party
persons.
Last updated: 06/03/11