Surveillance 1985 Vol. 12 No. 2 Page 3 Technique of bleeding pigs from the jugular vein Introduction safe alternative to the techniques mentioned above. This method consists of bleeding the pig in a standing position from the jugular vein. Taking satisfactory blood samples from pigs has, in the past, been a very daunting exercise. Ear and tail bleeding are time consuming and often the final sample leaves a lot to be desired. The alternative has been to take a blood sample from the anterior vena cava. This last method produces a very good sample and the technique has been well documented. However the possibility of having the pig drop dead on the end of the needle has deterred many people from using the technique. ‘The materials and equipment needed for this technique are simple and readily available. The animal is restrained by means of a rope secured through the mouth and around the upper jaw and snout. ‘The other end of the rope is wrapped around a post or railing (Figure 1). The objective of this article is to outline a very practical, The materials needed for bleeding the pig vary Equipment Continued overleaf Page 4 Surveillance 1985 Vol. 1 2 No. 2 Figure 1: Position for bleeding according to personal preference. The most successful apparatus is the common 10 ml vacutainer tube and holder with a 1% inch needle". This combination will be sufficient for most adult pigs, except in a small percentage of cases where the vein is deeper than 1 % inches, or in cases where the neck is shortened and the muscles are bunched because the animal is not leaning back against the snout rope. In these cases a 10 ml syringe and a 2% inch 14g needle will be sufficient to overcome the problem. A vacutainer needle-hub adaptor is available which can be used in combination with the normal vacutainer and a larger, longer needle; however, with this combination there is more risk of losing the vacuum in the tube because of the possibility of leakage at the needle-hub joint. :? Venoject 18gauge x 1I,% inch (1.2 x 38 mm) needle, Terumo. Catalogue number VN"1838. Supplied by Ebos Dental and Surgical Supplies Ltd. Technique The technique itself is simple. Only minimal restraint of the animal is required; the snout rope is quite sufficient. It is very important that the animal leans back against the pull of the rope because this straightens out the neck and prevents the neck muscles from bunching. Fortunately the pig has a natural tendency to pull against the rope and usually stands quite still for the whole procedure. The pig can be bled from either side depending on the personal preference of the bleeder. The best position for the bleeder is up against the pig's shoulder, either facing the same direction as the pig or with the back of the bleeder's legs against the shoulder of the animal. The vacutainer is held in the most convenient hand, in an inverted position with the tip of the index finger on the bottom of the vacutainer tube, as shown in Figure 2. 'The manner in which the vacutainer is held is very important because the bleeder is bent over and working very close t o the ground. By holding the tube in this way, the bleeder is allowed free movement and good control of the needle. Surveillance 1985 Vol. 12 No. 2 Page 5 Figure 2: Correct position of vacutainer Figure 3: Cross section of neck Point of entry of needle Once the bleeder is in position against the shoulder of the restrained pig, and the vacutainer and needle are properly held in the hand of choice, the bleeder bends down and places hisher free hand in front of the pig’s shoulder, with the fingertips in the jugular groove (Figure 1).It is questionable as to whether this free hand can actually reduce the flow of blood sufficiently to cause the jugular vein to fill any more than it already has, but this position is necessary to locate the site for inserting the needle. The jugular lies against the medial surface of the bulk of the muscles in the neck (Figure 3 ) . Therefore, placing the fingertips of the hand in the jugular groove will give the bleeder the approximate position of that medial surface. The needle is inserted with an upward thrust perpendicular to the plane of the ground and about half-way along the length of the neck. If the pig is leaning back and the neck is fully extended then the needle should enter approximately perpendicular to the neck as well. ’The jugular vein will usually be at the full depth of the 1 % inch needle. \ Conclusion This bleeding technique is usually simple and the stress on the animal and the bleeding crew is minimal. The principal disadvantage is that occasionally the needle will become blocked with a plug of fat. In many instances when this occurs, the needle has penetrated the jugular vein and damaged it. This results in a haematoma and the vein seems to collapse. If a larger gauge needle and syringe are used subsequently, then a sample can be obtained but it would be of doubtful quality for a haemogram study. It does seem that the larger the gauge of the needle the greater the chance of getting a tissue plug in it. C.J . Boland Veterinary Staff Officer Head Office
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