Control of cell division in Treponema denticola
[short, interesting and accurate]
Alpha N. Author1, Beta C. Second2 and Delta E. Third3*
1 University of Bristol, Lower Maudlin Street, Bristol, Somerset BS25 1DU, United Kingdom
2 Institute, Postal address (street or PO Box), City, Postal Code or Zip, Country
3 Institute, Postal address (street or PO Box), City, Zip, Country
Running head: T. denticola cell division
Keywords: Spirochaetes, periodontal disease, ftsZ, promoter structure
[Select 4-6 keywords carefully, remembering that these will be used by search engines. Do not
repeat words in the title.]
*Correspondence: Dr Delta Third, Department of Cell Biology, University of Florida, PO Box
1824, S 18th Street, Gainesville FL23456, USA
Tel: (+1) 978-654-321; E-mail: [email protected]
Word count = 6876 (including all tables, legends and references)
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SUMMARY
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[use line numbering]
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The Summary (n.b. not Abstract) is the most read part of a paper, so it is vital that it conveys the
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research question, how it has been addressed (= methods), the main results and the
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implications of the results. Write this part of the paper last. It should not be more than 250
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words. Avoid statements such as "the results are discussed". Use English (UK) spell-checker.
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End the Summary with sentences that capture the implications of the paper. This will define why
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the paper should be published in Molecular Oral Microbiology and read by an international
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audience.
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INTRODUCTION
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The first paragraph under all headings is not indented. The Introduction should introduce the
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subject and put it into current perspective. The result should be a short description of the state-
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of-the-art, suitably referenced. Avoid multiple citations, if possible. At the first mention of a
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microbial species in the main text (not Summary or title), it should be given its full Latin name
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with the current attribution. An example would be Treponema denticola. Subsequently, the
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organism can be referred to as T. denticola except when starting a sentence, when it should be
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given in full.
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The second and subsequent paragraphs are indented. References are numbered in
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consecutive order in the text, tables, or figures.Use superscript arabic numerals to cite material,
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e.g.,1
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End the Introduction with a clear description of the research question, supported by a
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hypothesis as appropriate. The following is an example. In order to determine the impact of T.
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denticola on the development of periodontitis, and to evaluate effects of these bacteria on other
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species present within lesions, a novel in vivo model was developed in conjunction with
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molecular analysis of defined-species biofilms. The hypothesis tested was that T. denticola
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influenced development of periodontal disease through activation of virulence factors expressed
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by other bacteria. The results suggest that proteases from Prevotella intermedia are activated
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by T. denticola. However, additional factors to protease activation are involved in induction of
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increased levels of pathogenesis by mixed-species communities.
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The first reference used in a written document is listed as 1 in the reference list.
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METHODS
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Second order heading is sentence case and bold
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to be able to repeat the work. As the author, you will be very familiar with what has been done,
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but the challenge is to present information clearly for others.
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Abbreviations should be written in full at first mention. Spellings should conform to those used
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in the Concise Oxford Dictionary. SI units should be used throughout. Consult the journal Author
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Guidelines (http://www.wiley.com/bw/submit.asp?ref=0043-1737).
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For unusual chemicals (e.g. pendimethalin), the product name (Stomp 400 SC) its concentration
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(400 g a.i. l-1) and the supplier (BASF plc) should be stated.
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Analysis
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Data were analysed formally with analysis of variance as a split-split plot design. All analyses
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were performed using the Genstat7 program2. The journal is preparing new guidelines on the
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RESULTS
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Second order heading is sentence case and bold
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Results should be separated from Discussion. Present the key analyzed results objectively. Do
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not repeat data in both tables and figures. Analyses of coverage between the sites showed
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some differences associated with the field size and substratum types (Table 1). There were no
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significant differences in growth rate associated with cultures with or without glucose.
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Third order heading is sentence case and bold
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Analysis of the species present in the microbiota indicated significantly greater biodiversity in
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the boundary of the margins adjacent to strips (Fig. 1), but no statistical difference between
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biomass measurements.
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DISCUSSION
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methods employed.
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ACKNOWLEDGEMENTS
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potential conflict of interests, if appropriate.
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REFERENCES
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1. Hu P, Reuben DB. Effects of managed care on the length of time that elderly patients spend
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with physicians during ambulatory visits. Med Care. 2002;40(7):606-613.
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2. Geller AC, Venna S, Prout M, et al. Should the skin cancer examination be taught in medical
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school? Arch Dermatol. 2002;138(9):1201-1203.
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3. Guyton JL, Crockarell JR. Fractures of acetabulum and pelvis. In: Canale ST, ed. Campbell's
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Operative Orthopaedics. 10th ed. Philadelphia, PA: Mosby, Inc; 2003:2939-2984.
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4. McKenzie BC. Medicine and the Internet: Introducing Online Resources and Terminology.
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2nd ed. New York, NY: Oxford University Press; 1997.
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Supporting Information
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Additional Supporting Information may be found in the online version of this article:
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Figure S1. Cell images in JPG format
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Figure S2. Photograph of apparatus used in the experiments
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Table S1. Data set of experiment one in Microsoft Excel format.
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Video Clip S1. Clip in Quicktime of cells taking up dye.
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Appendix S1. Detailed methodology (Word document)
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[Figure legends]
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Fig. 1 Canonical Correspondence Analysis ordination of the flora from the boundaries (), 6 m
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margins (o), crop edges () and crop centres () of paired arable fields in southern England. Data
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are based on mean species cover from three 5 m2 quadrants per location. The first two axes
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explain 39% of the species-environment relations.
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[Tables]
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Table 1 Bacterial strains used in this study and their derivation.