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Persoonia – Volume 36, 2016
Ganoderma ecuadoriense
441
Fungal Planet description sheets
Fungal Planet 461 – 4 July 2016
Ganoderma ecuadoriense W.A. Salazar, C.W. Barnes & Ordóñez, sp. nov.
Etymology. Name reflects the geographical origin from which the fungus
was collected.
Classification — Ganodermataceae, Polyporales, Agarico­
mycetes.
Basidiomata annual, flabelliform, pileate, pileus 21.6 × 21.8
mm, surface glabrous, woody, reddish brown, laccate, upper
surface covered by cinnamon coloured powder of deposited
basidiospores. Basidiospore surface smooth, white when fresh
and dark brown when dry, pores 5–7 per mm, round, thick walls.
Stipe missing from sample, but lateral. Hyphal system dimitic,
skeletal hyphae yellow to pale brown, 3.5 – 5.5 μm wide, end
in ramifications, generative hyphae thin-walled, hyaline to pale
yellow, 1–2.5 μm wide. Hyphae faintly amyloid when dispersed
and slightly dextrinoid when in masses with Melzer. Cuticule
cells club-like, slight amyloid reaction to 5 % KOH and Melzer.
Resin deposits between the trama and cuticle. Basidia not observed. Basidiospores hyaline to pale yellow, truncated, 8–10.5
× 4.5–7 μm, no reaction to 5 % KOH or Melzer.
Notes — Morphological identification using the Neotropical
Polyporaceae key (Ryvarden 2004), revealed G. ecuadoriense
to be very similar to G. perzonatum. However, based on a
Blastn ITS sequence comparison, after trimming the 18S and
28S sequences (Schoch et al. 2014), the highest similarities
were obtained with G. orbiforme from Brazil, and G. cupreum,
G. mastoporum and G. fornicatum from China. The sequence
of Ganoderma sp. VPB202 from Brazil is actually identical,
but has a low query score due to the discrepancy in sequence
length, missing roughly 25 bases at the 3’ end of ITS2. There
were seven consistent differences, three in ITS1 and four in
ITS2, between G. ecuadoriense, collected in the North-western
Amazon basin in Ecuador, and G. orbiforme, collected in the
South-eastern Amazon in Brazil. Twenty sequences, 16 for
G. ecuadoriense and four for G. orbiforme were used in the DNA
alignment analysis. The Ganoderma sp. VPB202 sequence
suggests G. ecuadoriense occurs throughout the Amazon
basin, but due to the missing bases of the ITS2 sequence, this
is somewhat speculative.
Typus. Ecuador, Orellana Province, Yasuní Research Station, on decaying
wood, Mar. 2013, A. Salazar (holotype QCAM3430, ITS sequence GenBank
KU128524, LSU sequence GenBank KX228350, TreeBASE Submission ID
18454, MycoBank MB816866).
G . perzonatum S P 445984 B R A
G . perzonatum S P 445985 B R A
G . cupreum G anoTK 7 C MR
83
92
100
G . orbiforme UR M83335 B R A
G . orbiforme UR M83336 B R A
G . orbiforme UR M83334 B R A
92
G anoderma sp. VP B 202 B R A
G . ecuadoriens e P oly-2-4 E C U
(type)
G . ecuadoriens e AS L 799 E C U (type)
G . ecuadoriens e P MC 126 E C U
79
G . fornicatum TNMF 0009926 C HN
G . cupreum HMAS 130804 C HN
Phylogenetic analysis was done using the Maximum Likelihood
plugin PHYML in Geneious v. 7.1 (http://www.geneious.com,
Kearse et al. 2012), and the substitution model HKY85 determined by jModelTest (Posada 2008) according to Corrected
Akaike Information Criterion (AICc). One hundred bootstrap
replicates were used. Included in the analysis were representative species found in the Blastn search, plus G. per­zonatum
sequences because of its morphological similarity. Sample
nomenclature: species name, isolate number, three letter United
Nations country code: AUS = Australia, BRA = Brazil, CHN =
China, CMR = Camaroon, ECU = Ecuador and MYS = Malaysia.
G . mastoporum TNMF 0018835 C HN
91
G . mastoporum TNMF 0018783 C HN
G . cupreum HMAS 99399 C HN
G . fornicatum B C R C 35374 C HN
69
G . cupreum DF P 4336 AUS
G . fornicatum TNMF 0010592 C HN
G . mastoporum F R IM98 MY S
G . cupreum DF P 3896 AUS
0.02
Colour illustrations. Ecuador, Yasuni National Park rain forest; basidio­
carps, skeletal and generative hyphae, basidiospores. Scale bars = 10 µm.
Washington A. Salazar, Maria E. Ordóñez, & Cristina Toapanta, Escuela de Ciencias Biológicas, Pontificia Universidad Católica del Ecuador,
Av. 12 de octubre 1076 y Roca, Quito, Ecuador;
e-mail: [email protected], [email protected] & [email protected]
Charles W. Barnes, Instituto Nacional Autónomo de Investigaciones Agropecuarias, Estación Experimental Santa Catalina,
Panamericana Sur Km 1, Sector Cutuglahua, Pichincha, Ecuador;
e-mail: [email protected]
Paul Gamboa, Universidad Central del Ecuador, Facultad de Medicina, Carrera de Ciencias Biológicas y Ambientales, Av. América, Quito, Ecuador;
e-mail: [email protected]
© 2016 Naturalis Biodiversity Center & Centraalbureau voor Schimmelcultures